BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,778,820	G→A	88.0%	intergenic (‑450/+301)	ypjC ← / ← ileY	pseudogene/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,778,820	0	G	A	88.0%	828.6 / 87.8	284	intergenic (‑450/+301)	ypjC/ileY	pseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand): ref base G (12/22); new base A (115/135); total (127/157)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

GCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAAT  >  CP009273/2778674‑2778968

  ctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                                  <  1:662739/149‑1 (MQ=255)

  ctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAAt                                                                                                                                                  <  1:389016/149‑1 (MQ=255)

          ggTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGa                                                                                                                                          <  1:2710467/149‑1 (MQ=255)

            tgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGAtt                                                                                                                                        <  2:1857215/149‑1 (MQ=255)

            tgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGAtt                                                                                                                                        >  2:1929821/1‑149 (MQ=255)

                cTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAAt                                                                                                                                    <  1:3841766/149‑1 (MQ=255)

                cTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAAt                                                                                                                                    <  1:2521077/149‑1 (MQ=255)

                 tGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATg                                                                                                                                   <  2:3868863/149‑1 (MQ=255)

                  ggTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGa                                                                                                                                  >  1:913493/1‑149 (MQ=255)

                     cAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAgg                                                                                                                               <  2:4443491/149‑1 (MQ=255)

                      agtagtAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAggg                                                                                                                              >  2:678710/1‑149 (MQ=255)

                       gtagtaGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTCAATGAAgggg                                                                                                                             >  2:3302428/1‑149 (MQ=255)

                       gtagtaGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAgggg                                                                                                                             <  1:1186401/149‑1 (MQ=255)

                            agATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataa                                                                                                                        >  1:4237990/1‑149 (MQ=255)

                                 tttAAGGCGTGTCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAAt                                                                                                                   <  2:4047690/149‑1 (MQ=255)

                                   tAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGa                                                                                                                 >  1:4725690/1‑149 (MQ=255)

                                         gTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATAc                                                                                                           <  1:3039445/149‑1 (MQ=255)

                                          tGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATAct                                                                                                          >  2:585734/1‑149 (MQ=255)

                                             cAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTg                                                                                                       <  2:2623557/149‑1 (MQ=255)

                                              agagACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTgg                                                                                                      <  1:4176070/149‑1 (MQ=255)

                                                 gaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCtt                                                                                                   >  1:1009630/1‑149 (MQ=255)

                                                  aCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCttt                                                                                                  >  2:197405/1‑149 (MQ=255)

                                                    aTTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTAt                                                                                                >  1:2887649/1‑149 (MQ=255)

                                                        cATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGtt                                                                                            >  2:4569179/1‑149 (MQ=255)

                                                        cATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGtt                                                                                            >  1:3719919/1‑149 (MQ=255)

                                                         aTCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTa                                                                                           <  1:1847524/149‑1 (MQ=255)

                                                         aTCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTa                                                                                           >  2:364497/1‑149 (MQ=255)

                                                         aTCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTa                                                                                           <  1:3189997/149‑1 (MQ=255)

                                                             ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTa                                                                                       >  2:1304694/1‑149 (MQ=255)

                                                             ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTa                                                                                       >  1:1377364/1‑149 (MQ=255)

                                                             ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTa                                                                                       >  2:4355380/1‑149 (MQ=255)

                                                                ctctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACtt                                                                                    >  1:154822/1‑149 (MQ=255)

                                                                ctctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACtt                                                                                    >  1:2231401/1‑149 (MQ=255)

                                                                 tctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTa                                                                                   <  1:4515270/149‑1 (MQ=255)

                                                                    aCGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAAtt                                                                                <  2:3720712/149‑1 (MQ=255)

                                                                    aCGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAAtt                                                                                <  1:128327/149‑1 (MQ=255)

                                                                     cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTc                                                                               <  2:1487411/149‑1 (MQ=255)

                                                                      ggCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTcc                                                                              >  1:3753066/1‑149 (MQ=255)

                                                                      ggCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTcc                                                                              <  2:3548995/149‑1 (MQ=255)

                                                                            gTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGt                                                                        <  1:2124028/149‑1 (MQ=255)

                                                                            gTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGt                                                                        <  2:4415735/149‑1 (MQ=255)

                                                                            gTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGt                                                                        >  2:2154911/1‑149 (MQ=255)

                                                                                tacatacaTTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGc                                                                    <  2:1840589/149‑1 (MQ=255)

                                                                                tacatacaTTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGc                                                                    >  2:230706/1‑149 (MQ=255)

                                                                                tacatacaTTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGc                                                                    <  2:4228956/149‑1 (MQ=255)

                                                                                tacatacaTTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGc                                                                    >  2:2746291/1‑149 (MQ=255)

                                                                                     acatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAttt                                                               >  1:4412798/1‑149 (MQ=255)

                                                                                     acatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAttt                                                               <  2:1791145/149‑1 (MQ=255)

                                                                                        ttGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGcc                                                            >  2:4704328/1‑149 (MQ=255)

                                                                                         tGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGccc                                                           <  1:2246756/149‑1 (MQ=255)

                                                                                           gTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGc                                                         >  2:1422819/1‑149 (MQ=255)

                                                                                            ttGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCt                                                        >  2:3529981/1‑149 (MQ=255)

                                                                                            gtgtgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCt                                                        <  2:1043848/148‑1 (MQ=255)

                                                                                             tgtgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCtt                                                       <  1:857015/149‑1 (MQ=255)

                                                                                              gtgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAAGAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTg                                                      <  2:289153/149‑1 (MQ=255)

                                                                                               tgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTgg                                                     >  2:4193531/1‑149 (MQ=255)

                                                                                                ggTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGc                                                    <  1:197405/149‑1 (MQ=255)

                                                                                                ggTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGc                                                    >  1:2644430/1‑149 (MQ=255)

                                                                                                 gTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTACACATGTAAGCAATTTGCCCGCTTGGCa                                                   >  2:2938831/1‑149 (MQ=255)

                                                                                                  tACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCAt                                                  >  2:2865768/1‑149 (MQ=255)

                                                                                                  tACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCAt                                                  <  1:1694303/149‑1 (MQ=255)

                                                                                                      cACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCa                                              >  1:280786/1‑149 (MQ=255)

                                                                                                      cACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCa                                              >  1:4165715/1‑149 (MQ=255)

                                                                                                        cTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAgg                                            <  1:759269/149‑1 (MQ=255)

                                                                                                          tATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCa                                          >  1:4218050/1‑149 (MQ=255)

                                                                                                           atcatcAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAt                                         >  1:2453407/1‑149 (MQ=255)

                                                                                                             catcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAttt                                       >  2:1796746/1‑149 (MQ=255)

                                                                                                              atcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtttt                                      <  1:3094604/149‑1 (MQ=255)

                                                                                                              atcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtttt                                      <  2:1565958/149‑1 (MQ=255)

                                                                                                                caGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtttttt                                    <  2:3215130/149‑1 (MQ=255)

                                                                                                                  gTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTcc                                  >  1:1314926/1‑149 (MQ=255)

                                                                                                                  gTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTcc                                  <  2:931147/149‑1 (MQ=255)

                                                                                                                  gTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTcc                                  <  2:2281040/149‑1 (MQ=255)

                                                                                                                    gAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAg                                >  2:729714/1‑149 (MQ=255)

                                                                                                                     aGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAgg                               <  2:1263054/149‑1 (MQ=255)

                                                                                                                      gCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCCCATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGt                              >  2:3757030/1‑149 (MQ=255)

                                                                                                                      gCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGt                              >  1:4060055/1‑149 (MQ=255)

                                                                                                                      gCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGt                              <  1:4741688/149‑1 (MQ=255)

                                                                                                                         aaCAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTGTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACt                           >  2:2555589/1‑149 (MQ=255)

                                                                                                                           cAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACttt                         <  1:1494073/149‑1 (MQ=255)

                                                                                                                             gagaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTg                       >  1:3932816/1‑149 (MQ=255)

                                                                                                                             gagaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTg                       >  1:2017615/1‑149 (MQ=255)

                                                                                                                               gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGaa                     <  1:3373197/149‑1 (MQ=255)

                                                                                                                               gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGaa                     <  1:1388809/149‑1 (MQ=255)

                                                                                                                                 aTAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATg                   >  1:831362/1‑149 (MQ=255)

                                                                                                                                    gTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGt                <  2:405761/149‑1 (MQ=255)

                                                                                                                                     ttCAGTGATTTGAATAATTAACCTGATTAAATGAAGTGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTa               <  2:2013474/149‑1 (MQ=255)

                                                                                                                                     ttCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTa               <  1:180505/149‑1 (MQ=255)

                                                                                                                                      tCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATTTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTTAATGAGTAc              >  2:3554709/1‑149 (MQ=255)

                                                                                                                                      tCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTAc              <  1:979472/149‑1 (MQ=255)

                                                                                                                                      tCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTACCAGGTACTTTTGAATGAGTAc              >  2:4234479/1‑149 (MQ=255)

                                                                                                                                         gTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGa           >  2:2989868/1‑149 (MQ=255)

                                                                                                                                         gTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGa           <  2:4517502/149‑1 (MQ=255)

                                                                                                                                             tttGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGa       >  2:2392033/1‑149 (MQ=255)

                                                                                                                                             tttGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGa       <  2:548267/149‑1 (MQ=255)

                                                                                                                                              ttGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAt      >  1:3740918/1‑149 (MQ=255)

                                                                                                                                               tGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATa     <  1:3066062/149‑1 (MQ=255)

                                                                                                                                               tGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATa     <  1:3324470/149‑1 (MQ=255)

                                                                                                                                               tGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATa     >  2:1176650/1‑149 (MQ=255)

                                                                                                                                                  gTACTTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACAGGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAAt  >  1:501739/1‑149 (MQ=255)

GCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAAT  >  CP009273/2778674‑2778968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`