Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,828,952 C→A 100% T611T (ACG→ACT)  hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,828,9520CA99.0% 818.3 / ‑1.4 268T611T (ACG→ACT) hypFcarbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (115/146);  total (117/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAAT  >  CP009273/2828806‑2829097
                                                                                                                                                  |                                                                                                                                                 
ggTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGt                                                                                                                                                 <  2:3067780/149‑1 (MQ=255)
   gcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggc                                                                                                                                              <  1:1430509/149‑1 (MQ=255)
    cctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggct                                                                                                                                             <  1:3834097/149‑1 (MQ=255)
     ctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctg                                                                                                                                            <  2:367977/149‑1 (MQ=255)
      tgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                           <  2:566534/149‑1 (MQ=255)
      tgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                           >  2:3355252/1‑149 (MQ=255)
         cAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGgcgc                                                                                                                                        <  2:1149967/149‑1 (MQ=255)
           gTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                      >  1:2145119/1‑149 (MQ=255)
              cAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGc                                                                                                                                   <  1:3978231/149‑1 (MQ=255)
                gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGccc                                                                                                                                 >  1:1140177/1‑149 (MQ=255)
                gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGccc                                                                                                                                 >  2:2719941/1‑149 (MQ=255)
                 ccACTGCTGCCAGACAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCTCCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                                <  2:1463626/149‑1 (MQ=255)
                 ccACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                                <  1:881888/149‑1 (MQ=255)
                 ccACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                                <  1:882414/149‑1 (MQ=255)
                  cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAg                                                                                                                               <  1:607219/149‑1 (MQ=255)
                  cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAg                                                                                                                               <  2:4042955/149‑1 (MQ=255)
                   aCTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                              <  1:4367534/149‑1 (MQ=255)
                   aCTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                              >  2:1024517/1‑149 (MQ=255)
                   aCTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                              <  2:2486/149‑1 (MQ=255)
                        tgcCAGAAAGTGTCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGTCTGCGAGCGCCTCCATAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgc                                                                                                                         <  1:1076427/149‑1 (MQ=255)
                        tgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgc                                                                                                                         >  2:2127835/1‑149 (MQ=255)
                        tgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgc                                                                                                                         <  2:2467364/149‑1 (MQ=255)
                          ccAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgccg                                                                                                                       >  1:243854/1‑149 (MQ=255)
                          ccAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgccg                                                                                                                       >  1:1512151/1‑149 (MQ=255)
                           cAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgccgc                                                                                                                      >  2:3923688/1‑149 (MQ=255)
                             gAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCa                                                                                                                    >  2:450324/1‑149 (MQ=255)
                              aaaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCAc                                                                                                                   >  1:1481252/1‑149 (MQ=255)
                              aaaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCAc                                                                                                                   <  1:4580942/149‑1 (MQ=255)
                                  tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCa                                                                                                               <  1:2294716/149‑1 (MQ=255)
                                  tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCa                                                                                                               <  1:3964553/149‑1 (MQ=255)
                                    gCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATc                                                                                                             <  1:4297117/149‑1 (MQ=255)
                                      gagaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                           <  2:35926/149‑1 (MQ=255)
                                      gagaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                           >  2:1956014/1‑149 (MQ=255)
                                       agaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaa                                                                                                          >  1:3719601/1‑149 (MQ=255)
                                            cAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAAc                                                                                                     <  2:3006231/149‑1 (MQ=255)
                                            cAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAAc                                                                                                     >  2:1650351/1‑149 (MQ=255)
                                             aGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                    >  2:4679599/1‑149 (MQ=255)
                                             aGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                    >  2:2868619/1‑149 (MQ=255)
                                             aGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                    >  2:3709412/1‑149 (MQ=255)
                                             aGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCATCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                    >  2:4620912/1‑149 (MQ=255)
                                              gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                   <  1:3755426/149‑1 (MQ=255)
                                              gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                   >  1:132235/1‑149 (MQ=255)
                                              gTTGATTGTCCACCCGCGGCAGTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                   <  2:4653492/149‑1 (MQ=255)
                                                tGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGccc                                                                                                 <  2:868210/149‑1 (MQ=255)
                                                         aCCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                        <  2:1045568/149‑1 (MQ=255)
                                                         aCCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGa                                                                                        <  1:2758281/149‑1 (MQ=255)
                                                          cccGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGAc                                                                                       <  2:400502/149‑1 (MQ=255)
                                                                     gTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGgcgc                                                                            >  2:288406/1‑149 (MQ=255)
                                                                       cACCGGTTGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGt                                                                          <  1:3002880/149‑1 (MQ=255)
                                                                            gATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAAtt                                                                     >  2:145936/1‑149 (MQ=255)
                                                                            gATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAAtt                                                                     <  1:4368097/149‑1 (MQ=255)
                                                                               gCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCa                                                                  <  2:2031756/149‑1 (MQ=255)
                                                                                 gTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACg                                                                >  1:260104/1‑149 (MQ=255)
                                                                                    aCTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTc                                                             <  1:197897/149‑1 (MQ=255)
                                                                                    aCTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTc                                                             <  1:3489933/149‑1 (MQ=255)
                                                                                    aCTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTc                                                             >  2:2768083/1‑149 (MQ=255)
                                                                                    aCTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATACCACGCTc                                                             >  2:762389/1‑149 (MQ=255)
                                                                                        cGTGGCATGAGGCTGCGAGCGCCTCCAGATCACAAGCCGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACGGCATCGAAAACACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATg                                                         <  2:4410883/149‑1 (MQ=255)
                                                                                         gTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATgg                                                        >  1:3191683/1‑149 (MQ=255)
                                                                                           gtcATGAGGCTGCGAGCGCCTCCAGAGAAAAAGCCGATACACATTCATAACTTAATGTGGCTGGCGAACTGCCCTGTTCCGCCGCCACTTCTTCTAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGcc                                                      <  1:4116103/147‑1 (MQ=255)
                                                                                           ggCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGcc                                                      >  1:1591395/1‑149 (MQ=255)
                                                                                           ggCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGcc                                                      <  2:3180856/149‑1 (MQ=255)
                                                                                           ggCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGcc                                                      <  1:340209/149‑1 (MQ=255)
                                                                                                 aGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGcc                                                <  2:588851/149‑1 (MQ=255)
                                                                                                     tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCa                                            <  1:1860013/149‑1 (MQ=255)
                                                                                                     tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCa                                            <  2:2920159/149‑1 (MQ=255)
                                                                                                      gCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCAc                                           <  2:1747092/149‑1 (MQ=255)
                                                                                                        gAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGc                                         <  1:2333322/149‑1 (MQ=255)
                                                                                                        gAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGc                                         <  1:3081174/149‑1 (MQ=255)
                                                                                                         aGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCt                                        >  2:3091239/1‑149 (MQ=255)
                                                                                                         aGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCt                                        <  2:2183408/149‑1 (MQ=255)
                                                                                                          gcgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                       >  2:529471/1‑149 (MQ=255)
                                                                                                          gcgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                       >  2:3400608/1‑149 (MQ=255)
                                                                                                           cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTcc                                      <  2:2374912/149‑1 (MQ=255)
                                                                                                            gcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCa                                     <  1:74326/149‑1 (MQ=255)
                                                                                                              cTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGt                                   <  1:4749950/149‑1 (MQ=255)
                                                                                                                ccAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                 >  2:3459127/1‑149 (MQ=255)
                                                                                                                   gagCACACGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACCGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                              <  1:3600443/149‑1 (MQ=255)
                                                                                                                   gagCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGCTGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                              <  2:1598879/149‑1 (MQ=255)
                                                                                                                   gagCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                              >  2:4752307/1‑149 (MQ=255)
                                                                                                                     gCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGAAGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTg                            >  1:1497683/1‑149 (MQ=255)
                                                                                                                       acaAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGtt                          <  1:2034197/149‑1 (MQ=255)
                                                                                                                       acaAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGtt                          >  1:604381/1‑149 (MQ=255)
                                                                                                                        caAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTg                         >  2:4089704/1‑149 (MQ=255)
                                                                                                                         aaGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGc                        <  2:1366854/149‑1 (MQ=255)
                                                                                                                          aGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAAAGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGca                       >  2:3859067/1‑149 (MQ=255)
                                                                                                                            ccGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcaca                     <  1:2819476/149‑1 (MQ=255)
                                                                                                                             cGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCacac                    >  1:2411352/1‑149 (MQ=255)
                                                                                                                               cTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACtt                  >  2:2316546/1‑149 (MQ=255)
                                                                                                                                 tCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc                >  2:1242049/1‑149 (MQ=255)
                                                                                                                                 tCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc                >  2:1490149/1‑149 (MQ=255)
                                                                                                                                 tCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGCTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc                <  1:2481392/149‑1 (MQ=255)
                                                                                                                                    ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGt             <  2:3761124/149‑1 (MQ=255)
                                                                                                                                     cTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGtt            >  2:4444165/1‑149 (MQ=255)
                                                                                                                                        cATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCa         <  1:4620912/149‑1 (MQ=255)
                                                                                                                                         aTAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag        >  2:3628810/1‑149 (MQ=255)
                                                                                                                                           aaCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCagag      >  2:639968/1‑149 (MQ=255)
                                                                                                                                              ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa   <  1:3594827/149‑1 (MQ=255)
                                                                                                                                              ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa   >  2:2572435/1‑149 (MQ=255)
                                                                                                                                               tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCTAAAAAACGCCCACACGATGACGCCAGCGGCGAGTTAAGTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTTCACACTTGCTGTTTCAGAGTAAt  <  1:4451870/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAAT  >  CP009273/2828806‑2829097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑