Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,094 C→A 100% P249Q (CCG→CAG)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,0940CA97.6% 811.8 / 8.1 249P249Q (CCG→CAG) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (130/113);  total (135/114)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

AATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAA  >  CP009273/2834949‑2835239
                                                                                                                                                 |                                                                                                                                                 
 atcaggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGaa                                                                                                                                               >  1:3551971/1‑149 (MQ=255)
  tcaggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAg                                                                                                                                              <  2:2969412/149‑1 (MQ=255)
    aggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGAt                                                                                                                                            <  1:1561186/149‑1 (MQ=255)
     ggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATg                                                                                                                                           >  1:4365423/1‑149 (MQ=255)
     ggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATg                                                                                                                                           >  2:4580178/1‑149 (MQ=255)
     ggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATg                                                                                                                                           <  1:1419115/149‑1 (MQ=255)
       atcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTc                                                                                                                                         <  1:3322267/149‑1 (MQ=255)
          aggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTgg                                                                                                                                      >  1:2802481/1‑149 (MQ=255)
          aggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTgg                                                                                                                                      >  2:2240958/1‑149 (MQ=255)
          aggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTgg                                                                                                                                      >  2:4602523/1‑149 (MQ=255)
          aggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTgg                                                                                                                                      >  1:3359113/1‑149 (MQ=255)
            gTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGggc                                                                                                                                    <  1:1324326/149‑1 (MQ=255)
               aaaTATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGggcggc                                                                                                                                 <  2:336970/149‑1 (MQ=255)
                 atatCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCTGGTGGGCTGTATGCTGGCGGGCGGTAACGTCTTCCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGgcgc                                                                                                                               >  2:1984362/1‑149 (MQ=255)
                 atatCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGgcgc                                                                                                                               <  1:1021735/149‑1 (MQ=255)
                  tatCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCt                                                                                                                              <  2:2387792/149‑1 (MQ=255)
                    tCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTgg                                                                                                                            >  1:2631656/1‑149 (MQ=255)
                    tCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCGGTATGCTGGCGGGCGGTCACTGCTACCCTGACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTgg                                                                                                                            >  1:732465/1‑149 (MQ=255)
                     cAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGga                                                                                                                           >  2:2048323/1‑149 (MQ=255)
                       ggCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGgaga                                                                                                                         <  1:3604224/149‑1 (MQ=255)
                       ggCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGgaga                                                                                                                         >  2:175495/1‑149 (MQ=255)
                         ccGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGaaa                                                                                                                       <  1:4466719/149‑1 (MQ=255)
                          cgcgCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAg                                                                                                                      >  1:598351/1‑149 (MQ=255)
                               aTCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCgg                                                                                                                 <  2:785622/149‑1 (MQ=255)
                               aTCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCgg                                                                                                                 <  2:3177051/149‑1 (MQ=255)
                                    cAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGaaaa                                                                                                            <  1:4602523/149‑1 (MQ=255)
                                     aGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAc                                                                                                           >  1:4056498/1‑149 (MQ=255)
                                      gCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAcc                                                                                                          >  1:2516207/1‑149 (MQ=255)
                                         ggTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGt                                                                                                       >  1:2297082/1‑149 (MQ=255)
                                         ggTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGt                                                                                                       >  1:4766136/1‑149 (MQ=255)
                                          gTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtt                                                                                                      <  1:1274465/149‑1 (MQ=255)
                                             gCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGttttt                                                                                                   >  2:719120/1‑149 (MQ=255)
                                             gCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGttttt                                                                                                   >  2:1775682/1‑149 (MQ=255)
                                              ccAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtttttt                                                                                                  <  1:1204543/149‑1 (MQ=255)
                                              ccAGTGCGCTAGCCACCAAAATCGCCAATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCtttttttt                                                                                                  <  1:1774658/149‑1 (MQ=255)
                                                aGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTAt                                                                                                <  1:4581005/149‑1 (MQ=255)
                                                 gTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATc                                                                                               <  1:1156440/149‑1 (MQ=255)
                                                 gTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATc                                                                                               >  1:2051011/1‑149 (MQ=255)
                                                    cgcTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGAt                                                                                            <  2:4165528/149‑1 (MQ=255)
                                                        aGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGc                                                                                        >  2:2360531/1‑149 (MQ=255)
                                                         gCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCa                                                                                       <  2:3633974/149‑1 (MQ=255)
                                                            accaAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGgc                                                                                    <  1:3891513/149‑1 (MQ=255)
                                                            accaAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGgc                                                                                    >  1:1123204/1‑149 (MQ=255)
                                                             ccaAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGACAACCTGTTTTTTATCGATGTGCAGgcg                                                                                   >  2:571339/1‑149 (MQ=255)
                                                                aactCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                                <  2:3289460/146‑1 (MQ=255)
                                                                aaaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                                <  1:4642811/149‑1 (MQ=255)
                                                                 aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCggg                                                                               >  1:1961092/1‑149 (MQ=255)
                                                                 aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCggg                                                                               >  1:4616287/1‑149 (MQ=255)
                                                                  aTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgggg                                                                              <  2:2420655/149‑1 (MQ=255)
                                                                  aTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgggg                                                                              >  2:3560006/1‑149 (MQ=255)
                                                                   tCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGc                                                                             >  2:1119689/1‑149 (MQ=255)
                                                                    cGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                            >  1:4301369/1‑149 (MQ=255)
                                                                    cGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                            >  1:2437245/1‑149 (MQ=255)
                                                                    cGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                            >  1:1051254/1‑149 (MQ=255)
                                                                    cGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                            >  1:1050510/1‑149 (MQ=255)
                                                                              gTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGc                                                                  >  2:2796602/1‑149 (MQ=255)
                                                                               ttAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCt                                                                 >  2:4093683/1‑149 (MQ=255)
                                                                               ttAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCt                                                                 <  1:569229/149‑1 (MQ=255)
                                                                                 aaCCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTa                                                               >  1:583258/1‑149 (MQ=255)
                                                                                    ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActc                                                            <  2:4079879/149‑1 (MQ=255)
                                                                                    ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActc                                                            <  2:2838672/149‑1 (MQ=255)
                                                                                    ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActc                                                            <  2:2838557/149‑1 (MQ=255)
                                                                                     cGCAAACTCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActct                                                           <  2:544611/149‑1 (MQ=255)
                                                                                     cGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActct                                                           >  2:2608286/1‑149 (MQ=255)
                                                                                      gCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTg                                                          >  2:1280508/1‑149 (MQ=255)
                                                                                        aaaaTCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGcc                                                        <  1:2791594/149‑1 (MQ=255)
                                                                                           aTCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCcgc                                                     <  1:206378/149‑1 (MQ=255)
                                                                                               ggTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTAt                                                 >  2:1434717/1‑149 (MQ=255)
                                                                                                 tGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTc                                               <  1:3606123/149‑1 (MQ=255)
                                                                                                      tGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGa                                          >  2:4040673/1‑149 (MQ=255)
                                                                                                        tATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGaac                                        >  2:4344095/1‑148 (MQ=255)
                                                                                                              ggcgggcgGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                                  <  1:3589572/149‑1 (MQ=255)
                                                                                                               gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGt                                 <  2:633803/149‑1 (MQ=255)
                                                                                                                 gggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTaa                               >  1:3772668/1‑149 (MQ=255)
                                                                                                                 gggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTaa                               >  1:1382746/1‑149 (MQ=255)
                                                                                                                 gggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGAATTCCGCGAAAAAGGGGTaa                               >  2:1468031/1‑149 (MQ=255)
                                                                                                                   gcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAAcc                             <  1:3600623/149‑1 (MQ=255)
                                                                                                                      gTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATc                          >  2:2402018/1‑149 (MQ=255)
                                                                                                                         aCTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaac                       >  1:4042231/1‑149 (MQ=255)
                                                                                                                         aCTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaac                       >  1:3286816/1‑149 (MQ=255)
                                                                                                                         aCTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaac                       >  1:3091965/1‑149 (MQ=255)
                                                                                                                           ttCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGATAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCGGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                     <  2:1146826/149‑1 (MQ=255)
                                                                                                                           ttCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                     >  1:4731204/1‑149 (MQ=255)
                                                                                                                           ttCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                     >  1:1836030/1‑149 (MQ=255)
                                                                                                                           ttCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                     >  1:838756/1‑149 (MQ=255)
                                                                                                                            tCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACaaa                    <  1:1809692/149‑1 (MQ=255)
                                                                                                                            tCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACaaa                    <  2:3772976/149‑1 (MQ=255)
                                                                                                                              tACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGc                  >  2:3861072/1‑149 (MQ=255)
                                                                                                                                cccTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCAc                <  2:1100373/149‑1 (MQ=255)
                                                                                                                                   ttACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCgg             <  1:3033995/149‑1 (MQ=255)
                                                                                                                                   ttACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCgg             <  2:2132481/149‑1 (MQ=255)
                                                                                                                                      cAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCg          <  2:3191518/149‑1 (MQ=255)
                                                                                                                                       aGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCga         <  2:2123363/149‑1 (MQ=255)
                                                                                                                                        gTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgat        <  1:3389297/149‑1 (MQ=255)
                                                                                                                                        gTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCG‑AAAAGGGGTAACCATCAACAAAGCACCGGGCgatg       <  2:845282/149‑1 (MQ=255)
                                                                                                                                         ttGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatg       >  2:2503244/1‑149 (MQ=255)
                                                                                                                                         ttGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatg       <  1:1325895/149‑1 (MQ=255)
                                                                                                                                          tGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatga      <  1:1636883/149‑1 (MQ=255)
                                                                                                                                            cAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGatgatg    >  1:3197695/1‑149 (MQ=255)
                                                                                                                                            cAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGatgatg    >  1:855098/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAA  >  CP009273/2834949‑2835239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑