Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,837,298 G→A 7.8% A91V (GCA→GTA)  hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,837,2980GA 7.8% 944.0 / 43.9 271A91V (GCA→GTA) hycGhydrogenase 3 and formate hydrogenase complex, HycG subunit
Reads supporting (aligned to +/- strand):  ref base G (102/147);  new base A (13/8);  total (115/156)
Fisher's exact test for biased strand distribution p-value = 6.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

AACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCG  >  CP009273/2837150‑2837446
                                                                                                                                                    |                                                                                                                                                    
aaCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTg                                                                                                                                                      >  1:2600569/1‑149 (MQ=255)
  cATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCa                                                                                                                                                    >  1:1207615/1‑149 (MQ=255)
   aTCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCAc                                                                                                                                                   >  2:1803427/1‑149 (MQ=255)
   aTCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCAc                                                                                                                                                   <  2:922014/149‑1 (MQ=255)
      cacaGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACggg                                                                                                                                                <  2:1385319/149‑1 (MQ=255)
       acaGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGt                                                                                                                                               <  2:1599717/149‑1 (MQ=255)
        caGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTg                                                                                                                                              <  1:2111709/149‑1 (MQ=255)
            gACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACcg                                                                                                                                          >  2:4506042/1‑149 (MQ=255)
              cAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACcgcg                                                                                                                                        >  2:2033891/1‑149 (MQ=255)
                   ttATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGt                                                                                                                                   <  1:4484100/149‑1 (MQ=255)
                     aTCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaa                                                                                                                                 >  2:75738/1‑149 (MQ=255)
                      tCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaaa                                                                                                                                <  2:641515/149‑1 (MQ=255)
                      tCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTACACGGGTGACCGCGCCGGTaaa                                                                                                                                >  1:722592/1‑149 (MQ=255)
                        cGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACa                                                                                                                              >  2:1316112/1‑149 (MQ=255)
                         gTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAg                                                                                                                             <  1:1738515/149‑1 (MQ=255)
                            ccGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTaa                                                                                                                          >  1:1721953/1‑149 (MQ=255)
                            ccGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTaa                                                                                                                          <  2:4643617/149‑1 (MQ=255)
                              gCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTaaaa                                                                                                                        >  1:2048697/1‑149 (MQ=255)
                                cccACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAata                                                                                                                      >  2:3471702/1‑149 (MQ=255)
                                cccACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAata                                                                                                                      <  1:2295297/149‑1 (MQ=255)
                                cccACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCAGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAata                                                                                                                      <  2:1974821/149‑1 (MQ=255)
                                  cacaCGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATc                                                                                                                    <  2:3640927/149‑1 (MQ=255)
                                  cacaCGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATc                                                                                                                    <  1:1041262/149‑1 (MQ=255)
                                  cacaCGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATc                                                                                                                    <  1:1865654/149‑1 (MQ=255)
                                   acacGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATcc                                                                                                                   <  1:3935664/149‑1 (MQ=255)
                                     acGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGc                                                                                                                 <  1:1060240/149‑1 (MQ=255)
                                      cGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCa                                                                                                                <  1:3105498/149‑1 (MQ=255)
                                      cGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTACACGGGTGACCGCGCCGGTCAACAGTAAAATATCCGCa                                                                                                                >  1:2146315/1‑149 (MQ=255)
                                      cGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTACACGGGTGACCGCGCCGGTACACAGTAAAATATCCGCa                                                                                                                >  1:4544273/1‑149 (MQ=255)
                                       gCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCAt                                                                                                               <  2:3553656/149‑1 (MQ=255)
                                           tAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGAcg                                                                                                           >  2:1480326/1‑149 (MQ=255)
                                               gaTCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGt                                                                                                       <  1:1026284/149‑1 (MQ=255)
                                               gaTCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGt                                                                                                       <  1:2513572/149‑1 (MQ=255)
                                                aTCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTg                                                                                                      <  1:1281064/149‑1 (MQ=255)
                                                  cGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGaa                                                                                                    <  2:2362053/149‑1 (MQ=255)
                                                  cGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGaa                                                                                                    <  2:3003444/149‑1 (MQ=255)
                                                    tGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAgg                                                                                                  <  2:3559087/149‑1 (MQ=255)
                                                    tGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAgg                                                                                                  <  1:4031487/149‑1 (MQ=255)
                                                    tGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAgg                                                                                                  <  1:1157012/149‑1 (MQ=255)
                                                      gAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGaa                                                                                                >  2:3150636/1‑149 (MQ=255)
                                                       aaGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAAc                                                                                               <  2:4193449/149‑1 (MQ=255)
                                                          aTCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGAc                                                                                            <  2:4092044/149‑1 (MQ=255)
                                                           tCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACt                                                                                           >  1:3349675/1‑149 (MQ=255)
                                                           tCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACt                                                                                           <  2:4441948/149‑1 (MQ=255)
                                                            cccGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACtt                                                                                          <  1:4668755/149‑1 (MQ=255)
                                                             ccGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACttt                                                                                         <  1:2105093/149‑1 (MQ=255)
                                                                ccACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAAt                                                                                      <  1:3481812/149‑1 (MQ=255)
                                                                ccACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAAt                                                                                      >  2:2545834/1‑149 (MQ=255)
                                                                   cTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGcc                                                                                   <  2:4186824/149‑1 (MQ=255)
                                                                     gTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTACACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGa                                                                                 >  1:2873712/1‑149 (MQ=255)
                                                                       tACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGCCGCGGTGAAGGAACGACTTTAATGCCGAAt                                                                               <  2:1229335/149‑1 (MQ=255)
                                                                       tACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAt                                                                               >  2:4585717/1‑149 (MQ=255)
                                                                          cGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGt                                                                            <  2:3272882/149‑1 (MQ=255)
                                                                          cGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGt                                                                            <  2:883631/149‑1 (MQ=255)
                                                                           gCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGtt                                                                           <  1:99640/149‑1 (MQ=255)
                                                                           gCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGtt                                                                           >  2:2784279/1‑149 (MQ=255)
                                                                           gCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGtt                                                                           <  1:4334708/149‑1 (MQ=255)
                                                                            cAGGCCCCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGAATTGCACGGGTGACCGCGCCGTTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTc                                                                          <  1:3216444/149‑1 (MQ=255)
                                                                            cAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGACCGACTTTAATGCCGAATCGTTc                                                                          >  1:825058/1‑149 (MQ=255)
                                                                              ggCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTg                                                                        >  2:1874263/1‑149 (MQ=255)
                                                                                cACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                      <  2:965323/149‑1 (MQ=255)
                                                                                 aCCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCAt                                                                     >  1:3038662/1‑149 (MQ=255)
                                                                                 aCCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCAt                                                                     >  1:1495121/1‑149 (MQ=255)
                                                                                   cGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCa                                                                   >  2:4228408/1‑149 (MQ=255)
                                                                                      aGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                >  2:4005745/1‑149 (MQ=255)
                                                                                      aGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                <  2:2703873/149‑1 (MQ=255)
                                                                                       ggAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                               >  1:1891614/1‑149 (MQ=255)
                                                                                         agaTACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                             >  1:3433439/1‑149 (MQ=255)
                                                                                            tACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcggc                                                          <  1:3784086/149‑1 (MQ=255)
                                                                                             aCAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcggcg                                                         <  1:583779/149‑1 (MQ=255)
                                                                                              cAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGa                                                        <  2:3926837/149‑1 (MQ=255)
                                                                                                aaTTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                      <  2:692593/149‑1 (MQ=255)
                                                                                                aaTTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTACACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                      <  2:2837887/149‑1 (MQ=255)
                                                                                                 aTTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAg                                                     <  2:539587/149‑1 (MQ=255)
                                                                                                 aTTGTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAg                                                     <  2:539581/149‑1 (MQ=255)
                                                                                                  ttttCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                    >  1:2923958/1‑149 (MQ=255)
                                                                                                     tCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                 <  2:2174497/149‑1 (MQ=255)
                                                                                                      cGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGACCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                <  2:1588192/149‑1 (MQ=255)
                                                                                                      cGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                >  1:2906441/1‑149 (MQ=255)
                                                                                                      cGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTACACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                <  1:2578064/149‑1 (MQ=255)
                                                                                                           ccGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                           >  1:4088373/1‑149 (MQ=255)
                                                                                                             ggCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAat                                         <  1:4226897/149‑1 (MQ=255)
                                                                                                             ggCGCGGACTGCCACGCACGCAGCGCAGGGGAGCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAat                                         >  2:492482/1‑149 (MQ=255)
                                                                                                              gcgcGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatt                                        <  1:4653912/149‑1 (MQ=255)
                                                                                                              gcgcGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatt                                        <  1:2636159/149‑1 (MQ=255)
                                                                                                                   gACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgat                                   >  1:1526301/1‑149 (MQ=255)
                                                                                                                   gACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgat                                   <  1:4733136/149‑1 (MQ=255)
                                                                                                                            gcacgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAcc                          <  1:3625505/149‑1 (MQ=255)
                                                                                                                              acgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGt                        >  1:1349590/1‑149 (MQ=255)
                                                                                                                                gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                      >  1:5198/1‑149 (MQ=255)
                                                                                                                                  agcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCa                    <  1:952751/149‑1 (MQ=255)
                                                                                                                                     gcagGGGATCGCATTGCACGTGTGACCGCGCCGTTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                 <  2:1045971/149‑1 (MQ=255)
                                                                                                                                        ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgcc              <  2:1920736/149‑1 (MQ=255)
                                                                                                                                         gggATCGCATTACACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGccgccg             >  1:662704/1‑149 (MQ=255)
                                                                                                                                          ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc            >  1:3266569/1‑149 (MQ=255)
                                                                                                                                           gATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCa           >  2:153817/1‑149 (MQ=255)
                                                                                                                                            aTCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg          <  1:3953461/149‑1 (MQ=255)
                                                                                                                                               gCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc       >  2:1203460/1‑149 (MQ=255)
                                                                                                                                                  ttGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg    >  2:3846375/1‑149 (MQ=255)
                                                                                                                                                  ttGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg    >  2:3714759/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCG  >  CP009273/2837150‑2837446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑