Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,841,044 C→A 100% A519A (GCG→GCT)  hycC ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,841,0440CA97.4% 1027.6 / 13.3 313A519A (GCG→GCT) hycChydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (8/0);  new base A (161/144);  total (169/144)
Fisher's exact test for biased strand distribution p-value = 8.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 313 total aligned reads displayed.

TTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCA  >  CP009273/2840898‑2841188
                                                                                                                                                  |                                                                                                                                                
ttGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGc                                                                                                                                                <  1:1510627/149‑1 (MQ=255)
  gCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCa                                                                                                                                              <  1:3463909/149‑1 (MQ=255)
   cGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCAt                                                                                                                                             <  1:1751609/149‑1 (MQ=255)
   cGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCAt                                                                                                                                             <  1:217650/149‑1 (MQ=255)
    gTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATa                                                                                                                                            >  2:1152988/1‑149 (MQ=255)
      aGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAat                                                                                                                                          >  2:82015/1‑149 (MQ=255)
         ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                       <  2:4327244/149‑1 (MQ=255)
         ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                       <  2:3701527/149‑1 (MQ=255)
         ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                       <  1:2875592/149‑1 (MQ=255)
         ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                       <  2:237533/149‑1 (MQ=255)
           cAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAAtgatga                                                                                                                                     >  2:1025655/1‑149 (MQ=255)
            aGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGaa                                                                                                                                    >  2:1254359/1‑149 (MQ=255)
               aCCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATgg                                                                                                                                 >  1:1645486/1‑149 (MQ=255)
                 cGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgca                                                                                                                               <  2:2610628/149‑1 (MQ=255)
                  ggCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcag                                                                                                                              >  1:558689/1‑149 (MQ=255)
                    cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagca                                                                                                                            <  1:1988702/149‑1 (MQ=255)
                      cAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCagcagc                                                                                                                          >  1:1336109/1‑149 (MQ=255)
                       aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCg                                                                                                                         >  1:1775907/1‑149 (MQ=255)
                       aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCg                                                                                                                         <  2:2498216/149‑1 (MQ=255)
                        aaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                        >  2:3169767/1‑149 (MQ=255)
                         aCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTAGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCggg                                                                                                                       >  2:4770125/1‑149 (MQ=255)
                          cGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggc                                                                                                                      >  2:4300692/1‑149 (MQ=255)
                           gCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggca                                                                                                                     >  2:662203/1‑149 (MQ=255)
                             cTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcagg                                                                                                                   >  2:1805944/1‑149 (MQ=255)
                               gTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggca                                                                                                                 >  2:2237523/1‑149 (MQ=255)
                               gTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggca                                                                                                                 >  1:2359864/1‑149 (MQ=255)
                                 ttCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAAt                                                                                                               >  1:1870431/1‑149 (MQ=255)
                                  tCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTAGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATACCCATAATGATGAATGGCAGCAGCGGGCAGGCAATc                                                                                                              >  2:4588720/1‑149 (MQ=255)
                                    aCCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                            >  1:3478234/1‑149 (MQ=255)
                                     gcGGCATGGCAAAACCGTGAGCGGTAATCCCCTGTGATTTTTCGTGGTCGTACCCGCACCCCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagc                                                                                                           <  2:2476676/148‑1 (MQ=255)
                                     ccGGAATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGTAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagc                                                                                                           <  1:25472/149‑1 (MQ=255)
                                       ggCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagcag                                                                                                         <  1:82015/149‑1 (MQ=255)
                                         cATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAgcagca                                                                                                       <  2:4140779/149‑1 (MQ=255)
                                          aTGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCaa                                                                                                      >  1:3727764/1‑149 (MQ=255)
                                            ggCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACg                                                                                                    <  1:2958011/149‑1 (MQ=255)
                                             gCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGt                                                                                                   <  2:3431449/149‑1 (MQ=255)
                                             gCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGt                                                                                                   >  1:4280109/1‑149 (MQ=255)
                                                aaaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGat                                                                                                >  1:4086972/1‑149 (MQ=255)
                                                    cGTGAGCGGTAATCACCATTGTTTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGTTCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatcatc                                                                                            <  2:1589703/149‑1 (MQ=255)
                                                     gTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCg                                                                                           >  1:4312809/1‑149 (MQ=255)
                                                          cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTga                                                                                      <  1:4007719/149‑1 (MQ=255)
                                                            gTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgaga                                                                                    >  2:1958392/1‑149 (MQ=255)
                                                                 cACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACgg                                                                               >  1:2027736/1‑149 (MQ=255)
                                                                  aCCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggt                                                                              >  1:4762598/1‑149 (MQ=255)
                                                                   ccATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCCATCAGCAGCAACGTGATCATCGGTTGAGAAACggtg                                                                             >  2:3091378/1‑149 (MQ=255)
                                                                   ccATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtg                                                                             <  2:4197184/149‑1 (MQ=255)
                                                                    cATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtgg                                                                            <  1:4692753/149‑1 (MQ=255)
                                                                    cATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtgg                                                                            <  1:729501/149‑1 (MQ=255)
                                                                    cATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtgg                                                                            >  2:3948157/1‑149 (MQ=255)
                                                                     aTTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtggt                                                                           <  2:3478234/149‑1 (MQ=255)
                                                                     aTTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtggt                                                                           <  1:2877764/149‑1 (MQ=255)
                                                                      ttGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGgtggtg                                                                          >  2:3261139/1‑149 (MQ=255)
                                                                      ttGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGgtggtg                                                                          >  2:872246/1‑149 (MQ=255)
                                                                        gATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGtt                                                                        <  2:1884722/149‑1 (MQ=255)
                                                                          tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAg                                                                      >  1:598112/1‑149 (MQ=255)
                                                                             ttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAg                                                                   <  2:4053601/149‑1 (MQ=255)
                                                                               cGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGc                                                                 <  1:3582776/149‑1 (MQ=255)
                                                                                  ggTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTcc                                                              <  2:2190309/149‑1 (MQ=255)
                                                                                  ggTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTcc                                                              >  1:1237076/1‑149 (MQ=255)
                                                                                     cGTAACCGCACACCCAGGGCGCAGCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCATCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                           <  2:4502957/149‑1 (MQ=255)
                                                                                      gTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCg                                                          >  2:512364/1‑149 (MQ=255)
                                                                                       tAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                         >  2:2662640/1‑149 (MQ=255)
                                                                                         aCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCa                                                       >  2:4576318/1‑149 (MQ=255)
                                                                                           cGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCaga                                                     >  1:4226379/1‑149 (MQ=255)
                                                                                              acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGt                                                  <  2:768487/149‑1 (MQ=255)
                                                                                               cacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                 <  1:1366788/149‑1 (MQ=255)
                                                                                                acCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAc                                                >  1:3321314/1‑149 (MQ=255)
                                                                                                     ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                           >  2:3506019/1‑149 (MQ=255)
                                                                                                     ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                           >  2:1228466/1‑149 (MQ=255)
                                                                                                     ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                           >  2:2568472/1‑149 (MQ=255)
                                                                                                         gCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCaga                                       >  2:275609/1‑149 (MQ=255)
                                                                                                           aCCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagaga                                     <  2:584220/149‑1 (MQ=255)
                                                                                                            ccGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagagag                                    >  1:2555744/1‑149 (MQ=255)
                                                                                                             cgcgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGc                                   >  2:443420/1‑149 (MQ=255)
                                                                                                               cgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCAt                                 <  1:2800294/149‑1 (MQ=255)
                                                                                                               cgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCAt                                 <  1:1025655/149‑1 (MQ=255)
                                                                                                                gggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATc                                >  1:3762842/1‑149 (MQ=255)
                                                                                                                 ggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                               >  1:1176749/1‑149 (MQ=255)
                                                                                                                 ggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                               >  1:1460950/1‑149 (MQ=255)
                                                                                                                 ggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                               >  2:3835862/1‑149 (MQ=255)
                                                                                                                    aCGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                            >  1:4652277/1‑149 (MQ=255)
                                                                                                                    aCGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                            >  1:393960/1‑149 (MQ=255)
                                                                                                                        gATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAg                        >  1:1112208/1‑149 (MQ=255)
                                                                                                                         aTGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGc                       <  1:3169767/149‑1 (MQ=255)
                                                                                                                         aTGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGc                       <  2:1555702/149‑1 (MQ=255)
                                                                                                                           ggCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCa                     >  1:3332221/1‑149 (MQ=255)
                                                                                                                                aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGTAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcg                >  1:2448205/1‑149 (MQ=255)
                                                                                                                                aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcg                >  2:2133600/1‑149 (MQ=255)
                                                                                                                                aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcg                >  2:4131454/1‑149 (MQ=255)
                                                                                                                                aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcg                >  1:2625480/1‑149 (MQ=255)
                                                                                                                                aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcg                >  2:1851639/1‑149 (MQ=255)
                                                                                                                                aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCACCGGCAGTAGCCACGgcg                >  2:4240934/1‑149 (MQ=255)
                                                                                                                                     cGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagca           <  1:3520422/149‑1 (MQ=255)
                                                                                                                                     cGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagca           >  2:1402402/1‑149 (MQ=255)
                                                                                                                                       ccTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac         >  2:28038/1‑149 (MQ=255)
                                                                                                                                       ccTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac         >  2:559939/1‑149 (MQ=255)
                                                                                                                                         tttGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAacac       <  2:2032658/149‑1 (MQ=255)
                                                                                                                                            gCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc    <  1:2755203/149‑1 (MQ=255)
                                                                                                                                            gCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc    >  2:3825439/1‑149 (MQ=255)
                                                                                                                                              aaaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCa  >  1:908913/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCA  >  CP009273/2840898‑2841188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑