Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,876,760 C→T 100% G246G (GGG→GGA)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,876,7600CT98.6% 973.4 / 2.0 281G246G (GGG→GGA) casACRISP RNA (crRNA) containing Cascade antiviral complex protein
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (131/146);  total (132/149)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 282 total aligned reads displayed.

TCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAATT  >  CP009273/2876614‑2876905
                                                                                                                                                  |                                                                                                                                                 
  cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGACTGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGGATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCaa                                                                                                                                               >  2:3159865/1‑149 (MQ=255)
  cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCaa                                                                                                                                               >  2:663693/1‑149 (MQ=255)
  cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCaa                                                                                                                                               <  1:2560801/149‑1 (MQ=255)
     aaCCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATgg                                                                                                                                            <  1:783123/149‑1 (MQ=255)
      aCCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATggg                                                                                                                                           >  1:1049379/1‑149 (MQ=255)
       ccTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGa                                                                                                                                          >  2:4574977/1‑149 (MQ=255)
        cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGAt                                                                                                                                         <  1:2891963/149‑1 (MQ=255)
           cccTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGc                                                                                                                                      >  2:3560554/1‑149 (MQ=255)
            ccTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCa                                                                                                                                     <  2:3872570/149‑1 (MQ=255)
            ccTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCa                                                                                                                                     >  1:4232770/1‑149 (MQ=255)
               ttCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATaa                                                                                                                                  >  1:1921225/1‑149 (MQ=255)
                tCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAt                                                                                                                                 <  2:4455383/149‑1 (MQ=255)
                tCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAt                                                                                                                                 >  2:1958071/1‑149 (MQ=255)
                 cTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAtt                                                                                                                                >  1:1336391/1‑149 (MQ=255)
                 cTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAtt                                                                                                                                <  2:2865374/149‑1 (MQ=255)
                 cTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAtt                                                                                                                                >  1:2689345/1‑149 (MQ=255)
                  ttGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTc                                                                                                                               >  2:3200318/1‑149 (MQ=255)
                    gACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCaa                                                                                                                             <  2:3950942/149‑1 (MQ=255)
                     aCTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAat                                                                                                                            >  1:1857969/1‑149 (MQ=255)
                      cTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAata                                                                                                                           >  1:2989261/1‑149 (MQ=255)
                       tGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAatat                                                                                                                          <  1:2113384/149‑1 (MQ=255)
                        gTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATg                                                                                                                         >  1:2237659/1‑149 (MQ=255)
                        gTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATg                                                                                                                         >  1:3853928/1‑149 (MQ=255)
                           aCCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                      <  1:926526/149‑1 (MQ=255)
                           aCCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                      <  1:4589550/149‑1 (MQ=255)
                                 cAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTg                                                                                                                >  1:3428934/1‑149 (MQ=255)
                                    ggggAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCa                                                                                                             >  2:4292794/1‑149 (MQ=255)
                                        aaTGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAAt                                                                                                         <  1:63040/149‑1 (MQ=255)
                                         aTGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATa                                                                                                        <  1:1774139/149‑1 (MQ=255)
                                         aTGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATa                                                                                                        <  1:4164602/149‑1 (MQ=255)
                                          tGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAg                                                                                                       >  1:1342239/1‑149 (MQ=255)
                                           gCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGa                                                                                                      <  1:1269586/149‑1 (MQ=255)
                                              gATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCa                                                                                                   <  1:3279483/149‑1 (MQ=255)
                                               aTGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCAc                                                                                                  <  1:4023396/149‑1 (MQ=255)
                                                 ggggCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACgg                                                                                                >  2:1173169/1‑149 (MQ=255)
                                                 ggggCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACgg                                                                                                <  1:1548858/149‑1 (MQ=255)
                                                    gCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACa                                                                                             >  2:3851186/1‑149 (MQ=255)
                                                      cATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACaaa                                                                                           >  2:2446985/1‑149 (MQ=255)
                                                      cATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACaaa                                                                                           >  1:4607135/1‑149 (MQ=255)
                                                      cATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACaaa                                                                                           >  1:1385090/1‑149 (MQ=255)
                                                        tAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAcc                                                                                         <  2:3608537/149‑1 (MQ=255)
                                                            ccATTAACTGTACAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAt                                                                                     <  2:2644888/149‑1 (MQ=255)
                                                            ccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAt                                                                                     <  2:598754/149‑1 (MQ=255)
                                                            ccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACAACGGACAAACCCAAt                                                                                     >  1:1652994/1‑149 (MQ=255)
                                                              aTTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTg                                                                                   >  2:4505670/1‑149 (MQ=255)
                                                               ttAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGa                                                                                  >  1:2194028/1‑149 (MQ=255)
                                                                tAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGAc                                                                                 <  1:4354822/149‑1 (MQ=255)
                                                                tAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGAc                                                                                 >  2:4382029/1‑149 (MQ=255)
                                                                  aCTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGa                                                                               <  2:2378980/149‑1 (MQ=255)
                                                                   cTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGaa                                                                              >  2:2571773/1‑149 (MQ=255)
                                                                      tAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCa                                                                           <  1:2449380/149‑1 (MQ=255)
                                                                       aaaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAg                                                                          <  2:3449202/149‑1 (MQ=255)
                                                                          ggTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGta                                                                       >  2:894020/1‑149 (MQ=255)
                                                                            tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGtata                                                                     <  1:1139525/149‑1 (MQ=255)
                                                                            tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGtata                                                                     <  1:31139/149‑1 (MQ=255)
                                                                            tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGtata                                                                     <  1:415160/149‑1 (MQ=255)
                                                                              aaTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATACTTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGa                                                                   >  2:2104816/1‑149 (MQ=255)
                                                                              aaTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGa                                                                   >  1:3924237/1‑149 (MQ=255)
                                                                                  taaccTTAAGAAAACCGGTATAACGCAAATTGCTATCCTGTCCACAGCAAGAACAATTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATACACAACGGACAAACCCAATTGACGAAGCAGGTCTAGACTCa                                                               >  2:641230/4‑149 (MQ=255)
                                                                                          aGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACtt                                                       >  1:1507242/1‑149 (MQ=255)
                                                                                           gAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTg                                                      >  1:437094/1‑149 (MQ=255)
                                                                                             aaaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGAt                                                    >  2:3999275/1‑149 (MQ=255)
                                                                                              aaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATa                                                   >  2:2244773/1‑149 (MQ=255)
                                                                                              aaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATa                                                   >  2:1825478/1‑149 (MQ=255)
                                                                                               aCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAg                                                  <  2:4091358/149‑1 (MQ=255)
                                                                                                     ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            >  1:4706532/1‑149 (MQ=255)
                                                                                                     ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            >  2:280818/1‑149 (MQ=255)
                                                                                                     ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            <  2:180458/149‑1 (MQ=255)
                                                                                                      taACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAAt                                           >  2:561943/1‑149 (MQ=255)
                                                                                                      taACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAAt                                           >  1:1001792/1‑149 (MQ=255)
                                                                                                      taACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAAt                                           <  1:63742/149‑1 (MQ=255)
                                                                                                       aaCGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATc                                          >  2:1627022/1‑149 (MQ=255)
                                                                                                        aCGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATcc                                         >  2:4748866/1‑149 (MQ=255)
                                                                                                          gCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCag                                       <  2:1921225/149‑1 (MQ=255)
                                                                                                               ttGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtagg                                  >  2:964636/1‑149 (MQ=255)
                                                                                                               ttGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtagg                                  >  2:3352853/1‑149 (MQ=255)
                                                                                                                 gCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCACTGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggtt                                <  2:2914626/149‑1 (MQ=255)
                                                                                                                 gCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggtt                                >  1:2952401/1‑149 (MQ=255)
                                                                                                                   tttCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggttgg                              >  1:3736686/1‑149 (MQ=255)
                                                                                                                      ccTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           <  2:156724/149‑1 (MQ=255)
                                                                                                                      ccTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           <  1:585363/149‑1 (MQ=255)
                                                                                                                         gTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTcc                        >  1:508673/1‑149 (MQ=255)
                                                                                                                          tCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCg                       <  1:2353478/149‑1 (MQ=255)
                                                                                                                            cacaGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTa                     >  1:4616299/1‑149 (MQ=255)
                                                                                                                            cacaGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTa                     <  1:3192360/149‑1 (MQ=255)
                                                                                                                               aGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgt                  <  1:4538743/149‑1 (MQ=255)
                                                                                                                                gCAAGAACATTTACCAATTCCCATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgtg                 <  2:770142/149‑1 (MQ=255)
                                                                                                                                  aaGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGAt               >  1:3525773/1‑149 (MQ=255)
                                                                                                                                    gAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc             >  1:3097438/1‑149 (MQ=255)
                                                                                                                                    gAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc             >  1:3834523/1‑149 (MQ=255)
                                                                                                                                        aTTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTa         >  2:4354448/1‑149 (MQ=255)
                                                                                                                                        aTTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTa         >  2:3027939/1‑149 (MQ=255)
                                                                                                                                         tttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAg        <  2:4405283/149‑1 (MQ=255)
                                                                                                                                         tttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAg        <  2:456054/149‑1 (MQ=255)
                                                                                                                                           tACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGa      >  1:4309902/1‑149 (MQ=255)
                                                                                                                                            aCCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGaa     <  2:825740/149‑1 (MQ=255)
                                                                                                                                             ccAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGaaa    <  1:2606525/149‑1 (MQ=255)
                                                                                                                                               aaTTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAtt  <  1:3047881/149‑1 (MQ=255)
                                                                                                                                               aaTTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAtt  <  1:3851195/149‑1 (MQ=255)
                                                                                                                                               aaTTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAtt  <  2:2097122/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAATT  >  CP009273/2876614‑2876905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑