Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,884,385 G→T 84.9% V291V (GTC→GTA)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,884,3850GT84.9% 600.5 / 120.7 263V291V (GTC→GTA) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (8/32);  new base T (95/128);  total (103/160)
Fisher's exact test for biased strand distribution p-value = 7.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTA  >  CP009273/2884239‑2884529
                                                                                                                                                  |                                                                                                                                                
    cACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGt                                                                                                                                            >  1:799156/1‑149 (MQ=255)
      cTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTc                                                                                                                                          <  1:1009930/149‑1 (MQ=255)
         cGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCttt                                                                                                                                       <  2:3835223/149‑1 (MQ=255)
         cGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCttt                                                                                                                                       <  1:3447336/149‑1 (MQ=255)
         cGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCttt                                                                                                                                       <  1:3447306/149‑1 (MQ=255)
          gggTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTc                                                                                                                                      >  1:676120/1‑149 (MQ=255)
          gggTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCCTTCAGCCACAGCAGTTCTACAAGTTCTTTc                                                                                                                                      <  2:2895297/149‑1 (MQ=255)
           ggTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCa                                                                                                                                     >  1:2879658/1‑149 (MQ=255)
                  gTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGc                                                                                                                              <  2:3591859/149‑1 (MQ=255)
                  gTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGc                                                                                                                              <  2:2821643/149‑1 (MQ=255)
                   tGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGcc                                                                                                                             <  1:1735409/149‑1 (MQ=255)
                   tGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGcc                                                                                                                             <  1:620645/149‑1 (MQ=255)
                   tGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGcc                                                                                                                             <  1:422230/149‑1 (MQ=255)
                    ggCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCg                                                                                                                            >  1:4184710/1‑149 (MQ=255)
                       gTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGAt                                                                                                                         <  2:1149448/149‑1 (MQ=255)
                       gTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGAt                                                                                                                         <  2:3673928/149‑1 (MQ=255)
                           ttCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGtt                                                                                                                     <  2:311050/149‑1 (MQ=255)
                           ttCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGtt                                                                                                                     <  1:2893089/149‑1 (MQ=255)
                             ctcAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCt                                                                                                                   <  2:1837012/149‑1 (MQ=255)
                             ctcAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCt                                                                                                                   <  1:4064657/149‑1 (MQ=255)
                                aacaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGAt                                                                                                                <  2:1116878/149‑1 (MQ=255)
                                  caaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATAc                                                                                                              >  1:2666427/1‑149 (MQ=255)
                                  caaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATAc                                                                                                              >  1:2458945/1‑149 (MQ=255)
                                    aTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCa                                                                                                            >  1:2597667/1‑149 (MQ=255)
                                    aTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCa                                                                                                            <  1:311723/149‑1 (MQ=255)
                                      gTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGa                                                                                                          <  2:2682239/149‑1 (MQ=255)
                                       ttGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGAc                                                                                                         <  2:4716741/149‑1 (MQ=255)
                                          gCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGcc                                                                                                      <  1:4317191/149‑1 (MQ=255)
                                           cGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTGCAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGccc                                                                                                     <  1:387692/149‑1 (MQ=255)
                                           cGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGccc                                                                                                     <  1:1293742/149‑1 (MQ=255)
                                            ggTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCa                                                                                                    >  2:2796587/1‑149 (MQ=255)
                                            ggTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCa                                                                                                    <  1:3706877/149‑1 (MQ=255)
                                                  gACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGt                                                                                              >  2:686762/1‑149 (MQ=255)
                                                  gACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGt                                                                                              >  1:367365/1‑149 (MQ=255)
                                                    cGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCa                                                                                            <  2:382683/149‑1 (MQ=255)
                                                    cGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCa                                                                                            >  1:411966/1‑149 (MQ=255)
                                                     ggTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAc                                                                                           <  1:3642366/149‑1 (MQ=255)
                                                     ggTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAc                                                                                           >  1:1363727/1‑149 (MQ=255)
                                                       tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAccc                                                                                         <  1:2722132/149‑1 (MQ=255)
                                                       tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAccc                                                                                         <  1:457091/149‑1 (MQ=255)
                                                              gAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGt                                                                                  >  1:2007669/1‑149 (MQ=255)
                                                               aaGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTa                                                                                 >  1:486954/1‑149 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTaa                                                                                >  1:2054942/1‑149 (MQ=255)
                                                                 gTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAAc                                                                               <  2:4256265/149‑1 (MQ=255)
                                                                    ccACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCa                                                                            >  1:2517447/1‑149 (MQ=255)
                                                                    ccACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCa                                                                            <  1:1790224/149‑1 (MQ=255)
                                                                     cACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCAt                                                                           >  1:196471/1‑149 (MQ=255)
                                                                     cACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCAt                                                                           <  1:4696986/149‑1 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                    >  2:2077220/1‑149 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                    <  1:4664732/149‑1 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                    >  2:4110781/1‑149 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                    <  1:3651813/149‑1 (MQ=255)
                                                                            gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                    >  1:907129/1‑149 (MQ=255)
                                                                               cTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCa                                                                 >  2:3469799/1‑149 (MQ=255)
                                                                               cTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCa                                                                 >  1:881957/1‑149 (MQ=255)
                                                                               cTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCa                                                                 >  2:762189/1‑149 (MQ=255)
                                                                                ttCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCAc                                                                >  2:2576535/1‑149 (MQ=255)
                                                                                 tCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCAcc                                                               <  1:2093780/149‑1 (MQ=255)
                                                                                 tCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCAcc                                                               <  1:4492774/149‑1 (MQ=255)
                                                                                 tCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCAcc                                                               <  2:1655832/149‑1 (MQ=255)
                                                                                   gTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTa                                                             <  1:394407/149‑1 (MQ=255)
                                                                                    ttCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTaa                                                            <  2:1667513/149‑1 (MQ=255)
                                                                                    ttCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTaa                                                            >  1:3547022/1‑149 (MQ=255)
                                                                                      cAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGt                                                          <  1:1316791/149‑1 (MQ=255)
                                                                                      cAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGt                                                          <  2:3287627/149‑1 (MQ=255)
                                                                                       agagGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTc                                                         >  2:2972104/1‑149 (MQ=255)
                                                                                         agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                       <  1:77389/149‑1 (MQ=255)
                                                                                         agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                       <  1:125409/149‑1 (MQ=255)
                                                                                              aCGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCg                                                  >  1:730240/1‑149 (MQ=255)
                                                                                               cGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGa                                                 <  1:2343150/149‑1 (MQ=255)
                                                                                                gTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGat                                                >  2:836392/1‑149 (MQ=255)
                                                                                                 ttttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                               <  1:1726714/149‑1 (MQ=255)
                                                                                                 ttttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                               <  2:3659477/149‑1 (MQ=255)
                                                                                                   ttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATg                                             >  2:4146873/1‑149 (MQ=255)
                                                                                                     gCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGc                                           <  2:1479542/149‑1 (MQ=255)
                                                                                                     gCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGc                                           <  2:1479172/149‑1 (MQ=255)
                                                                                                      cccTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCg                                          >  1:2252439/1‑149 (MQ=255)
                                                                                                         tCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAAc                                       >  1:3738783/1‑149 (MQ=255)
                                                                                                                  aCAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTc                              <  2:2298353/149‑1 (MQ=255)
                                                                                                                    aGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCag                            >  2:1504325/1‑149 (MQ=255)
                                                                                                                     ggTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCaga                           >  2:2767933/1‑149 (MQ=255)
                                                                                                                      gTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGACACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCagag                          >  1:3706080/1‑149 (MQ=255)
                                                                                                                       ttCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                         <  1:1111226/149‑1 (MQ=255)
                                                                                                                       ttCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                         <  1:1851762/149‑1 (MQ=255)
                                                                                                                       ttCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                         <  2:2486064/149‑1 (MQ=255)
                                                                                                                          aTCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTAc                      <  1:4440588/149‑1 (MQ=255)
                                                                                                                           tCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTaag                     >  1:2991266/1‑147 (MQ=255)
                                                                                                                             gCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGcc                   <  1:435371/149‑1 (MQ=255)
                                                                                                                               cTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCg                 >  1:4090902/1‑149 (MQ=255)
                                                                                                                                tttCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCgg                >  2:3514984/1‑149 (MQ=255)
                                                                                                                                    aGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAt            <  2:633159/149‑1 (MQ=255)
                                                                                                                                     gCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGATTTACGCCCGGTaaat           >  2:503904/1‑147 (MQ=255)
                                                                                                                                     gCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtt           >  1:2329431/1‑149 (MQ=255)
                                                                                                                                     gCCACAGCAGTTCTACAAGTTATTTCACCATTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACACGGCTGGTAACGCATGCCCGAGTCTCATAAGTCAATTTCGATATGGAGAAAGTCTTTTTCAGAGTTACGACCGGTAAtt           >  1:1504208/1‑149 (MQ=255)
                                                                                                                                       cacaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         >  2:3624557/1‑149 (MQ=255)
                                                                                                                                       cacaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         >  2:1234796/1‑149 (MQ=255)
                                                                                                                                       cacaGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         >  1:3336784/1‑149 (MQ=255)
                                                                                                                                            cagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGt    <  2:1017648/149‑1 (MQ=255)
                                                                                                                                             atttCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGtt   <  1:32341/147‑1 (MQ=255)
                                                                                                                                              gTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTa  >  2:3525980/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTA  >  CP009273/2884239‑2884529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑