BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,889,129	C→A	88.4%	A4S (GCA→TCA)	ygcQ ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,889,129	0	C	A	88.4%	737.3 / 95.7	284	A4S (GCA→TCA)	ygcQ	putative flavoprotein
Reads supporting (aligned to +/- strand): ref base C (14/19); new base A (108/143); total (122/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 287 total aligned reads displayed.

CCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGG  >  CP009273/2888983‑2889274

  aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGata                                                                                                                                               >  1:1138206/1‑149 (MQ=255)

   cTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGatat                                                                                                                                              <  1:945840/149‑1 (MQ=255)

   cTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGatat                                                                                                                                              <  1:3622815/149‑1 (MQ=255)

   cTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGatat                                                                                                                                              <  1:1375210/149‑1 (MQ=255)

    tGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATAtt                                                                                                                                             <  2:2933375/149‑1 (MQ=255)

     gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTc                                                                                                                                            <  1:597735/149‑1 (MQ=255)

     gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTc                                                                                                                                            <  1:2923091/149‑1 (MQ=255)

       tCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCAt                                                                                                                                          <  2:1627768/149‑1 (MQ=255)

       tCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCAt                                                                                                                                          <  2:1372872/149‑1 (MQ=255)

         caccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATgg                                                                                                                                        <  2:1110135/149‑1 (MQ=255)

         caccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATgg                                                                                                                                        >  1:857698/1‑149 (MQ=255)

            caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAAGTGAGATATTCATGGCTg                                                                                                                                     >  1:2453565/1‑149 (MQ=255)

             aaCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGc                                                                                                                                    >  1:3474866/1‑149 (MQ=255)

               cGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCAt                                                                                                                                  >  2:1442438/1‑149 (MQ=255)

                 cATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATcc                                                                                                                                <  2:2611392/149‑1 (MQ=255)

                 cATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATcc                                                                                                                                <  1:1716914/149‑1 (MQ=255)

                 cATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATcc                                                                                                                                <  1:3452554/149‑1 (MQ=255)

                   tCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCtt                                                                                                                              <  1:4583733/149‑1 (MQ=255)

                     cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTg                                                                                                                            <  1:563258/149‑1 (MQ=255)

                         aCCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGt                                                                                                                        <  1:768139/149‑1 (MQ=255)

                              ttCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATa                                                                                                                   <  2:1847475/149‑1 (MQ=255)

                              ttCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATa                                                                                                                   >  2:3060369/1‑149 (MQ=255)

                               tCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAg                                                                                                                  <  1:314698/149‑1 (MQ=255)

                                ccGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGt                                                                                                                 <  2:10455/149‑1 (MQ=255)

                                   ccACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGTGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCt                                                                                                              <  1:722292/149‑1 (MQ=255)

                                   ccACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCt                                                                                                              <  1:927844/149‑1 (MQ=255)

                                    cACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTg                                                                                                             >  1:4211011/1‑149 (MQ=255)

                                        aCAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATATTCCTGCCAt                                                                                                         >  1:4001803/1‑149 (MQ=255)

                                        aCAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCAt                                                                                                         >  1:4751504/1‑149 (MQ=255)

                                             cTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtt                                                                                                    >  2:1139397/1‑149 (MQ=255)

                                             cTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtt                                                                                                    >  2:2234712/1‑149 (MQ=255)

                                               gAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtttt                                                                                                  >  1:1313059/1‑149 (MQ=255)

                                               gAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtttt                                                                                                  <  1:2741304/149‑1 (MQ=255)

                                               gAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCtttt                                                                                                  <  1:2311219/149‑1 (MQ=255)

                                                aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTg                                                                                                 >  1:1206676/1‑149 (MQ=255)

                                                  gTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAg                                                                                               <  2:2657969/149‑1 (MQ=255)

                                                  gTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCGTTTGAg                                                                                               >  2:49870/1‑149 (MQ=255)

                                                   ttCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGc                                                                                              >  1:2674985/1‑149 (MQ=255)

                                                      aaTCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCttt                                                                                           >  2:3994845/1‑149 (MQ=255)

                                                       aTCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCtttt                                                                                          <  1:4723890/149‑1 (MQ=255)

                                                         cTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTc                                                                                        <  2:1026277/149‑1 (MQ=255)

                                                         cTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTc                                                                                        <  1:1548764/149‑1 (MQ=255)

                                                          tGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCt                                                                                       <  2:474263/149‑1 (MQ=255)

                                                               gTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTAc                                                                                  >  1:840004/1‑149 (MQ=255)

                                                               gTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTAc                                                                                  >  1:2490123/1‑149 (MQ=255)

                                                                tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTAcc                                                                                 <  2:3940437/149‑1 (MQ=255)

                                                                      aGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGc                                                                           >  1:3416174/1‑149 (MQ=255)

                                                                         gTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCa                                                                        <  2:1394060/149‑1 (MQ=255)

                                                                         gTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCa                                                                        <  2:3212756/149‑1 (MQ=255)

                                                                          tACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCat                                                                       <  2:3568785/149‑1 (MQ=255)

                                                                           aCAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatc                                                                      <  2:594373/149‑1 (MQ=255)

                                                                           aCAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatc                                                                      <  2:3166522/149‑1 (MQ=255)

                                                                            cAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatca                                                                     >  1:713016/1‑149 (MQ=255)

                                                                            cAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatca                                                                     >  1:1948505/1‑149 (MQ=255)

                                                                             aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaa                                                                    <  2:1466943/149‑1 (MQ=255)

                                                                             aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaa                                                                    >  1:3793869/1‑149 (MQ=255)

                                                                               ccGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCatcaatc                                                                  <  2:4200155/149‑1 (MQ=255)

                                                                                   tGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCg                                                              <  2:2960546/149‑1 (MQ=255)

                                                                                   tGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCg                                                              >  1:425903/1‑149 (MQ=255)

                                                                                    gAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGt                                                             >  1:280213/1‑149 (MQ=255)

                                                                                       aTCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGc                                                          >  1:3292860/1‑149 (MQ=255)

                                                                                                 gccagccaGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTACATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGcgtcgt                                                <  1:2374764/149‑1 (MQ=255)

                                                                                                   cagccagcTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtg                                              >  1:4595749/1‑149 (MQ=255)

                                                                                                     gccagcTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct                                            >  1:1851021/1‑149 (MQ=255)

                                                                                                     gccagcTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct                                            >  1:503275/1‑149 (MQ=255)

                                                                                                       cagcTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctct                                          <  2:214440/149‑1 (MQ=255)

                                                                                                        agcTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctctg                                         >  1:1059426/1‑149 (MQ=255)

                                                                                                           ttGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctctgctc                                      >  2:4458133/1‑149 (MQ=255)

                                                                                                                aTTGCGGCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGcc                                 <  2:977143/149‑1 (MQ=255)

                                                                                                                     ggCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCATTTGGCGTAAATATTCCTGCCATAGATTTTGAGCTTTTTATGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTg                            >  2:2520082/1‑149 (MQ=255)

                                                                                                                      gCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGc                           <  2:3049330/149‑1 (MQ=255)

                                                                                                                      gCATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTACTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGATGCTCTGCTCTTGCCAGATGc                           >  2:2909012/1‑149 (MQ=255)

                                                                                                                       cATTCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCa                          <  1:3848353/149‑1 (MQ=255)

                                                                                                                         ttCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGa                        <  2:2037226/149‑1 (MQ=255)

                                                                                                                         ttCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGa                        >  1:4419551/1‑149 (MQ=255)

                                                                                                                         ttCTCCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGa                        >  2:3123524/1‑149 (MQ=255)

                                                                                                                          tctcCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGac                       <  1:3020366/149‑1 (MQ=255)

                                                                                                                          tctcCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGac                       <  1:629836/149‑1 (MQ=255)

                                                                                                                            tcCTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGacac                     <  2:232699/149‑1 (MQ=255)

                                                                                                                              cTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTg                   >  2:4299627/1‑149 (MQ=255)

                                                                                                                              cTGATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTg                   >  2:3401754/1‑149 (MQ=255)

                                                                                                                                gATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCa                 <  1:4060637/149‑1 (MQ=255)

                                                                                                                                gATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCa                 <  1:1772566/149‑1 (MQ=255)

                                                                                                                                gATTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTCCa                 >  2:4100703/1‑149 (MQ=255)

                                                                                                                                gATTTATTGTCACAAGTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCa                 <  2:4640552/149‑1 (MQ=255)

                                                                                                                                 aTTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCAt                <  1:2097801/149‑1 (MQ=255)

                                                                                                                                 aTTTATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCAt                <  1:1442438/149‑1 (MQ=255)

                                                                                                                                  tttATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATc               >  2:729575/1‑149 (MQ=255)

                                                                                                                                  tttATTGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATc               <  1:4307383/149‑1 (MQ=255)

                                                                                                                                      ttGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTg           >  1:1730085/1‑149 (MQ=255)

                                                                                                                                      ttGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTg           >  2:2725450/1‑149 (MQ=255)

                                                                                                                                      ttGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTg           <  1:4143521/149‑1 (MQ=255)

                                                                                                                                      ttGTCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTg           >  2:4474817/1‑149 (MQ=255)

                                                                                                                                         tCACAATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCCGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCa        <  2:1775746/149‑1 (MQ=255)

                                                                                                                                          cacaATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCAt       <  2:2414059/149‑1 (MQ=255)

                                                                                                                                          cacaATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCAt       <  2:1281561/149‑1 (MQ=255)

                                                                                                                                            caATTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCAttt     >  1:3337250/1‑149 (MQ=255)

                                                                                                                                              aTTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCg   >  2:4387495/1‑149 (MQ=255)

                                                                                                                                              aTTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCg   >  1:4490160/1‑149 (MQ=255)

                                                                                                                                              aTTGAGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCg   <  1:3768398/149‑1 (MQ=255)

CCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGG  >  CP009273/2888983‑2889274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`