Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,893,446 T→A 100% K63* (AAA→TAA)  ygcW ← putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,893,4460TA99.4% 1054.0 / ‑2.8 307K63* (AAA→TAA) ygcWputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (144/161);  total (144/163)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 314 total aligned reads displayed.

CCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAG  >  CP009273/2893299‑2893591
                                                                                                                                                   |                                                                                                                                                 
 cAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGttatt                                                                                                                                                 <  1:814717/149‑1 (MQ=255)
  aGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTc                                                                                                                                                <  2:162576/149‑1 (MQ=255)
  aGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTc                                                                                                                                                <  2:4276136/149‑1 (MQ=255)
    cACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCaa                                                                                                                                              <  1:1811530/149‑1 (MQ=255)
     aCCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAAt                                                                                                                                             >  1:4494316/1‑149 (MQ=255)
        ttATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAt                                                                                                                                          <  1:4270754/149‑1 (MQ=255)
        ttATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAt                                                                                                                                          <  2:1794228/149‑1 (MQ=255)
         tATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAtt                                                                                                                                         <  1:1028671/149‑1 (MQ=255)
          aTTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAttt                                                                                                                                        <  2:3693621/149‑1 (MQ=255)
           ttCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGTAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTATGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTc                                                                                                                                       <  2:1686395/149‑1 (MQ=255)
            tCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTcc                                                                                                                                      <  1:3866554/149‑1 (MQ=255)
             cAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCt                                                                                                                                     <  1:603747/149‑1 (MQ=255)
              aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCtt                                                                                                                                    >  1:4067061/1‑149 (MQ=255)
              aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCtt                                                                                                                                    >  2:3851578/1‑149 (MQ=255)
                cTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTg                                                                                                                                  <  2:64384/149‑1 (MQ=255)
                     aaaTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCg                                                                                                                             >  1:2145051/1‑149 (MQ=255)
                       aTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGcc                                                                                                                           <  2:1128899/149‑1 (MQ=255)
                        tACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCg                                                                                                                          >  2:1765977/1‑149 (MQ=255)
                         aCCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGt                                                                                                                         <  1:3929385/149‑1 (MQ=255)
                          ccGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGtt                                                                                                                        <  1:1957469/149‑1 (MQ=255)
                            ggCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGttatt                                                                                                                                                 >  1:2981564/1‑122 (MQ=255)
                            ggCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGttatt                                                                                                                                                 <  2:2981564/122‑1 (MQ=255)
                              cATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCt                                                                                                                    >  1:4724031/1‑149 (MQ=255)
                              cATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCt                                                                                                                    <  1:959637/149‑1 (MQ=255)
                               aTTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCtt                                                                                                                   <  2:3538435/149‑1 (MQ=255)
                                ttGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                                                  <  1:2613073/149‑1 (MQ=255)
                                ttGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                                                  >  1:4127755/1‑149 (MQ=255)
                                 tGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTg                                                                                                                 <  1:76610/149‑1 (MQ=255)
                                  gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGa                                                                                                                >  2:4430490/1‑149 (MQ=255)
                                  gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGa                                                                                                                <  2:284499/149‑1 (MQ=255)
                                      aCCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaa                                                                                                            >  1:1247942/1‑149 (MQ=255)
                                       ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCACTCATTTCCTTTGTTTCGCCGTTATCTTTGACGaaa                                                                                                           >  2:4152643/1‑149 (MQ=255)
                                       ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaaa                                                                                                           >  2:1339009/1‑149 (MQ=255)
                                          gAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTa                                                                                                        <  2:4352687/149‑1 (MQ=255)
                                            atatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAgg                                                                                                      <  2:4783653/149‑1 (MQ=255)
                                            atatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAgg                                                                                                      <  1:4050395/149‑1 (MQ=255)
                                               tCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAAt                                                                                                   <  2:3097185/149‑1 (MQ=255)
                                                  aCTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATaaa                                                                                                <  1:144454/149‑1 (MQ=255)
                                                   cTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAg                                                                                               <  2:2820972/149‑1 (MQ=255)
                                                    tGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGa                                                                                              >  2:1723183/1‑149 (MQ=255)
                                                       aCCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGatat                                                                                           <  1:450653/149‑1 (MQ=255)
                                                        ccGAAACGCTCACCGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATAtt                                                                                          <  2:2689184/149‑1 (MQ=255)
                                                          gAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                                        >  1:1022042/1‑149 (MQ=255)
                                                              cGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGcc                                                                                    <  2:1919155/149‑1 (MQ=255)
                                                               gCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCa                                                                                   <  2:3193151/149‑1 (MQ=255)
                                                               gCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCa                                                                                   >  2:2997520/1‑149 (MQ=255)
                                                                  cacaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGACTAAAGATATTTGCGCCAGCt                                                                                >  1:1440030/1‑149 (MQ=255)
                                                                  cacaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCt                                                                                <  1:1665514/149‑1 (MQ=255)
                                                                  cacaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCt                                                                                >  2:1210092/1‑149 (MQ=255)
                                                                   acaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCtt                                                                               <  1:1403644/149‑1 (MQ=255)
                                                                   acaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCtt                                                                               <  1:1767570/149‑1 (MQ=255)
                                                                     agcaagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTg                                                                             <  1:2485202/149‑1 (MQ=255)
                                                                        aagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGcc                                                                          <  1:1733916/149‑1 (MQ=255)
                                                                         agcagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCa                                                                         >  1:659306/1‑149 (MQ=255)
                                                                         agcagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCa                                                                         >  1:1489078/1‑149 (MQ=255)
                                                                          gcagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCaa                                                                        <  2:3280023/149‑1 (MQ=255)
                                                                                aTAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCAt                                                                  >  2:4060396/1‑149 (MQ=255)
                                                                                   aTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGc                                                               >  2:70507/1‑149 (MQ=255)
                                                                                   aTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGc                                                               <  1:2586645/149‑1 (MQ=255)
                                                                                   aTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGc                                                               >  2:3504559/1‑149 (MQ=255)
                                                                                    tcttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCa                                                              >  1:702726/1‑149 (MQ=255)
                                                                                    tcttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCa                                                              >  1:4551333/1‑149 (MQ=255)
                                                                                            ggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTg                                                      <  1:2551191/149‑1 (MQ=255)
                                                                                            ggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTg                                                      >  2:3334352/1‑149 (MQ=255)
                                                                                               gcgcCTTCTGCGGTGATACCCACCTGCATGAAGTACACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGcc                                                   <  1:2125969/149‑1 (MQ=255)
                                                                                                cgcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCt                                                  <  1:1673577/149‑1 (MQ=255)
                                                                                                    ttCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAAc                                              >  2:1528853/1‑149 (MQ=255)
                                                                                                        gCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCt                                          <  1:1157745/149‑1 (MQ=255)
                                                                                                           gTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTAtt                                       >  2:3978685/1‑149 (MQ=255)
                                                                                                             gATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTcc                                     >  1:2735571/1‑149 (MQ=255)
                                                                                                              aTACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTccc                                    >  1:3282145/1‑149 (MQ=255)
                                                                                                                   cACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCgg                               <  2:2210510/149‑1 (MQ=255)
                                                                                                                     ccTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTa                             <  2:2358040/149‑1 (MQ=255)
                                                                                                                        gCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACa                          >  1:1534033/1‑149 (MQ=255)
                                                                                                                         cATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACaa                         <  2:2511607/149‑1 (MQ=255)
                                                                                                                         cATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACaa                         <  1:3737462/149‑1 (MQ=255)
                                                                                                                          aTGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAt                        <  1:2687209/149‑1 (MQ=255)
                                                                                                                            gAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTg                      <  2:3904757/149‑1 (MQ=255)
                                                                                                                            gAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTg                      <  1:4739103/149‑1 (MQ=255)
                                                                                                                               gTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCgg                   >  1:2182945/1‑149 (MQ=255)
                                                                                                                               gTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAACATATATGCGCCAGCTATGGCCAACGCCATGTCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCgg                   >  1:3531507/1‑149 (MQ=255)
                                                                                                                                tCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGt                  <  1:2213713/149‑1 (MQ=255)
                                                                                                                                tCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGt                  <  1:4705547/149‑1 (MQ=255)
                                                                                                                                 ccACCTCAACCCCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGtt                 <  2:2797731/149‑1 (MQ=255)
                                                                                                                                 ccACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGtt                 <  1:3069141/149‑1 (MQ=255)
                                                                                                                                  cACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt                <  2:485631/149‑1 (MQ=255)
                                                                                                                                  cACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt                >  1:2325056/1‑149 (MQ=255)
                                                                                                                                     cTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc             >  2:3123074/1‑149 (MQ=255)
                                                                                                                                       cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCt           <  2:2400740/149‑1 (MQ=255)
                                                                                                                                       cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCt           >  2:4182941/1‑149 (MQ=255)
                                                                                                                                       cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCt           <  1:2771717/149‑1 (MQ=255)
                                                                                                                                        aaCACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCtt          >  2:4533801/1‑149 (MQ=255)
                                                                                                                                         acacCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCttt         >  2:2441826/1‑149 (MQ=255)
                                                                                                                                         acacCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCttt         <  1:3227535/149‑1 (MQ=255)
                                                                                                                                            cccTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAg      >  1:3835288/1‑149 (MQ=255)
                                                                                                                                             ccTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAgg     >  1:3413737/1‑149 (MQ=255)
                                                                                                                                             ccTGTTATTCAATCATTTCCCTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAgg     >  2:1867719/1‑149 (MQ=255)
                                                                                                                                              cTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAggg    >  1:3544244/1‑149 (MQ=255)
                                                                                                                                              cTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAggg    >  2:88909/1‑149 (MQ=255)
                                                                                                                                                gTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGgag  >  1:1851956/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAG  >  CP009273/2893299‑2893591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑