Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,908,287 C→A 37.9% D25Y (GAC→TAC)  rlmD ← 23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,908,2870CA37.9% 374.1 / 284.5 277D25Y (GAC→TAC) rlmD23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (85/87);  new base A (49/56);  total (134/143)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 278 total aligned reads displayed.

GGCTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGC  >  CP009273/2908140‑2908434
                                                                                                                                                   |                                                                                                                                                   
ggCTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCg                                                                                                                                                    >  1:4654623/1‑149 (MQ=255)
ggCTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCg                                                                                                                                                    >  2:4687818/1‑149 (MQ=255)
ggCTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCg                                                                                                                                                    <  2:2011755/149‑1 (MQ=255)
 gCTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGa                                                                                                                                                   >  2:1427239/1‑149 (MQ=255)
  cTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAg                                                                                                                                                  <  1:758088/149‑1 (MQ=255)
    aTCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGt                                                                                                                                                <  2:3032324/149‑1 (MQ=255)
     tCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTc                                                                                                                                               >  2:2074404/1‑149 (MQ=255)
     tCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTc                                                                                                                                               <  1:422222/149‑1 (MQ=255)
      cGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCg                                                                                                                                              <  1:3643479/149‑1 (MQ=255)
       gCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGt                                                                                                                                             <  1:3702191/149‑1 (MQ=255)
       gCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGt                                                                                                                                             >  2:3504319/1‑149 (MQ=255)
        cTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGtt                                                                                                                                            <  2:1131347/149‑1 (MQ=255)
         ttAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTg                                                                                                                                           <  1:138100/149‑1 (MQ=255)
         ttAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTg                                                                                                                                           >  1:381650/1‑149 (MQ=255)
           aaCCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGAc                                                                                                                                         <  2:4040115/149‑1 (MQ=255)
                   tACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACgg                                                                                                                                 >  2:572514/1‑149 (MQ=255)
                   tACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACgg                                                                                                                                 >  2:830081/1‑149 (MQ=255)
                     cgtcTTTAGCGCGGGCATAATGTTTTTTATATTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGtt                                                                                                                               <  2:4734808/146‑1 (MQ=255)
                        cTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATg                                                                                                                            >  1:2714235/1‑149 (MQ=255)
                        cTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATg                                                                                                                            <  1:3158817/149‑1 (MQ=255)
                          ttAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGAt                                                                                                                          <  2:4379277/149‑1 (MQ=255)
                          ttAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGAt                                                                                                                          <  2:4379312/149‑1 (MQ=255)
                               gcgGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGAc                                                                                                                     <  1:4342016/149‑1 (MQ=255)
                                 gggCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGAcgc                                                                                                                   >  2:733707/1‑149 (MQ=255)
                                 gggCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGAcgc                                                                                                                   <  1:969393/149‑1 (MQ=255)
                                  ggCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGAcgcg                                                                                                                  >  1:1871638/1‑149 (MQ=255)
                                      tACTGATTTTTATATTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGcgtcgt                                                                                                              <  1:2494255/149‑1 (MQ=255)
                                        cTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGcgtcgtcg                                                                                                            >  2:3188871/1‑149 (MQ=255)
                                         tGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGcgtcgtcgt                                                                                                           >  2:297509/1‑149 (MQ=255)
                                         tGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGcgtcgtcgt                                                                                                           <  1:781308/149‑1 (MQ=255)
                                         tGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGcgtcgtcgt                                                                                                           >  2:2410663/1‑149 (MQ=255)
                                           ttttttATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGc                                                                                                         >  1:1029224/1‑149 (MQ=255)
                                              tttATATTAAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAATAGAGTCGATGTCGTTGACTGAAACTGTTATGATCTGACGCGTCGTCGTTcgtc                                                                                                      <  1:4172676/149‑1 (MQ=255)
                                                tATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGcgtcgt                                                                                                    <  2:1869428/149‑1 (MQ=255)
                                                  tCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGttt                                                                                                  <  2:4202620/149‑1 (MQ=255)
                                                   cTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGtttt                                                                                                 >  2:3430390/1‑149 (MQ=255)
                                                   cTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGtttt                                                                                                 <  1:4394244/149‑1 (MQ=255)
                                                   cTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGtttt                                                                                                 <  1:1486228/149‑1 (MQ=255)
                                                    ttCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTg                                                                                                >  1:1724784/1‑149 (MQ=255)
                                                     tCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGc                                                                                               <  1:525517/149‑1 (MQ=255)
                                                       agtaacagtaacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCag                                                                                             >  2:3273022/1‑149 (MQ=255)
                                                       agtaacagtaacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCag                                                                                             >  2:3840912/1‑149 (MQ=255)
                                                        gtaacagtaacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCaga                                                                                            <  2:2834104/149‑1 (MQ=255)
                                                          aacagtaacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTGGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGt                                                                                          >  1:3362207/1‑149 (MQ=255)
                                                          aacagtaacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGt                                                                                          <  1:3472614/149‑1 (MQ=255)
                                                              gtaacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGaa                                                                                      >  2:1207134/1‑149 (MQ=255)
                                                               taacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAAt                                                                                     >  1:1136676/1‑149 (MQ=255)
                                                                aacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAAtt                                                                                    <  1:93820/149‑1 (MQ=255)
                                                                aacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAAtt                                                                                    <  1:2657987/149‑1 (MQ=255)
                                                                aacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAAtt                                                                                    >  1:1314828/1‑149 (MQ=255)
                                                                aacTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAAtt                                                                                    <  2:3547703/149‑1 (MQ=255)
                                                                  cTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTgc                                                                                  >  1:3402099/1‑149 (MQ=255)
                                                                     ccGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGcc                                                                               >  2:3915409/1‑149 (MQ=255)
                                                                      cgcgTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCa                                                                              <  2:780613/149‑1 (MQ=255)
                                                                         gTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTg                                                                           >  1:3213607/1‑149 (MQ=255)
                                                                          ttttCCTGCGGCTATAATCCGGTTATATATAGCGTTTTGCCGTGATGTCGCGCCACGCCCTTACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACTCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATggg                                                                          <  2:541882/149‑1 (MQ=255)
                                                                          ttttCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTgg                                                                          <  1:2303072/149‑1 (MQ=255)
                                                                           tttCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGc                                                                         <  2:2427362/149‑1 (MQ=255)
                                                                            ttCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCg                                                                        <  2:3323991/149‑1 (MQ=255)
                                                                             tCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCga                                                                       <  2:2643954/149‑1 (MQ=255)
                                                                             tCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCga                                                                       <  2:456361/149‑1 (MQ=255)
                                                                                tGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGAc                                                                    >  2:2208454/1‑149 (MQ=255)
                                                                                 gCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACt                                                                   >  2:4501460/1‑149 (MQ=255)
                                                                                  cGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACtt                                                                  <  2:4239579/149‑1 (MQ=255)
                                                                                      aataatCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTtctc                                                              >  1:2498548/1‑149 (MQ=255)
                                                                                       ataatCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCa                                                             >  1:4348727/1‑149 (MQ=255)
                                                                                        taatCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCaa                                                            >  1:3557992/1‑149 (MQ=255)
                                                                                         aatCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAAt                                                           >  2:918990/1‑149 (MQ=255)
                                                                                              ggggATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAAc                                                      <  2:2358671/149‑1 (MQ=255)
                                                                                               gggATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACa                                                     >  1:4524937/1‑149 (MQ=255)
                                                                                                ggATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAg                                                    <  1:2996369/149‑1 (MQ=255)
                                                                                                 gATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAgt                                                   <  1:1996049/149‑1 (MQ=255)
                                                                                                  aTAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAgtg                                                  >  2:3675017/1‑149 (MQ=255)
                                                                                                   tAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAgtgt                                                 >  2:2318420/1‑149 (MQ=255)
                                                                                                   tAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAgtgt                                                 >  2:4116397/1‑149 (MQ=255)
                                                                                                     aaTAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGa                                               >  2:713174/1‑149 (MQ=255)
                                                                                                       tAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGAcc                                             >  1:326565/1‑149 (MQ=255)
                                                                                                        aGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCt                                            <  1:4115913/149‑1 (MQ=255)
                                                                                                           gTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTaa                                         <  1:1615810/149‑1 (MQ=255)
                                                                                                           gTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTaa                                         >  1:4705229/1‑149 (MQ=255)
                                                                                                            ttttGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAAt                                        >  1:1346949/1‑149 (MQ=255)
                                                                                                               tGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGt                                     <  2:3788558/149‑1 (MQ=255)
                                                                                                                  cGTTATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTccc                                  >  2:1691370/1‑149 (MQ=255)
                                                                                                                   gTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCa                                 >  1:3140724/1‑149 (MQ=255)
                                                                                                                    ttATGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCAt                                >  1:283813/1‑149 (MQ=255)
                                                                                                                     tATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAATCGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATa                               <  1:1445340/149‑1 (MQ=255)
                                                                                                                     tATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATa                               <  1:3834788/149‑1 (MQ=255)
                                                                                                                      aTGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATaa                              >  2:2151166/1‑149 (MQ=255)
                                                                                                                      aTGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATaa                              <  1:3970790/149‑1 (MQ=255)
                                                                                                                      aTGTCGCGCCACGCCCTGACCAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATaa                              >  1:4736943/1‑149 (MQ=255)
                                                                                                                           gcgcCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGaa                         >  1:4015512/1‑149 (MQ=255)
                                                                                                                                   cccTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGa                 <  1:2487126/149‑1 (MQ=255)
                                                                                                                                   cccTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGa                 >  2:2750807/1‑149 (MQ=255)
                                                                                                                                       gACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCaa             <  2:4654623/149‑1 (MQ=255)
                                                                                                                                          cAAAAGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTa          >  2:50334/1‑149 (MQ=255)
                                                                                                                                           aaaaGAGTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTAc         <  1:4301086/149‑1 (MQ=255)
                                                                                                                                            aaaGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACa        <  1:237497/149‑1 (MQ=255)
                                                                                                                                              agagTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGc      <  1:2876919/149‑1 (MQ=255)
                                                                                                                                              agagTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGc      <  2:629529/149‑1 (MQ=255)
                                                                                                                                               gagTAGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGcc     <  1:479414/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCTATCGCTTAACCGGCGTACGACTTTAGCGCGGGCATACTGTTTTTTATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCCTGACCAAAAGAGTCGAGGTCGTTGACTGAAACGGTTATGATCTGACGCGTCGTCGTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGC  >  CP009273/2908140‑2908434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑