Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,917,972 C→T 10.6% intergenic (‑89/+122) csrB ← / ← syd CsrA‑binding sRNA, antagonizing CsrA regulation/SecY‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,917,9720CT10.6% 1095.9 / 85.1 351intergenic (‑89/+122)csrB/sydCsrA‑binding sRNA, antagonizing CsrA regulation/SecY‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (140/170);  new base T (16/21);  total (157/194)
Fisher's exact test for biased strand distribution p-value = 8.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 354 total aligned reads displayed.

TGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAG  >  CP009273/2917824‑2918120
                                                                                                                                                    |                                                                                                                                                    
   gtCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAAt                                                                                                                                                   <  1:4233978/149‑1 (MQ=255)
        gCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACa                                                                                                                                              <  2:1882138/149‑1 (MQ=255)
        gCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACa                                                                                                                                              <  1:1928563/149‑1 (MQ=255)
          aGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAtt                                                                                                                                            >  1:3769618/1‑149 (MQ=255)
          aGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAtt                                                                                                                                            <  1:2478598/149‑1 (MQ=255)
          aGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAtt                                                                                                                                            <  1:755125/149‑1 (MQ=255)
          aGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAtt                                                                                                                                            >  1:648693/1‑149 (MQ=255)
           gAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAttt                                                                                                                                           <  2:3329650/149‑1 (MQ=255)
            aaGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACAtttt                                                                                                                                          <  1:2621729/149‑1 (MQ=255)
             aGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTg                                                                                                                                         <  1:1140487/149‑1 (MQ=255)
             aGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTg                                                                                                                                         >  1:4193042/1‑149 (MQ=255)
              gtgtCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGa                                                                                                                                        <  1:1012715/149‑1 (MQ=255)
               tgtCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGAt                                                                                                                                       <  2:1243318/149‑1 (MQ=255)
                gtCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGAtt                                                                                                                                      <  2:1273728/149‑1 (MQ=255)
                 tcatcaTCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGAttt                                                                                                                                     >  1:28357/1‑149 (MQ=255)
                 tcatcaTCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGAttt                                                                                                                                     >  2:2301933/1‑149 (MQ=255)
                 tcatcaTCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGAttt                                                                                                                                     <  2:2886129/149‑1 (MQ=255)
                  catcatCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGAtttt                                                                                                                                    >  2:673544/1‑149 (MQ=255)
                    tcatcCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTaa                                                                                                                                  >  1:4390975/1‑149 (MQ=255)
                    tcatcCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTaa                                                                                                                                  >  2:2012257/1‑149 (MQ=255)
                       tcCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATg                                                                                                                               <  1:289127/149‑1 (MQ=255)
                        ccTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGa                                                                                                                              <  2:2657872/149‑1 (MQ=255)
                         cTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAAAAATCAACATTTTGATTTTAAATTaa                                                                                                                             >  2:4590995/1‑149 (MQ=255)
                           gATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGaaaa                                                                                                                           <  2:3994056/149‑1 (MQ=255)
                               ttCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAAc                                                                                                                       <  2:593699/149‑1 (MQ=255)
                                tctcTTCGTTGTCTGACTCCCTGTCGACGAAGAGAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACg                                                                                                                      <  2:4231243/146‑1 (MQ=255)
                                 cACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGa                                                                                                                     <  2:401919/149‑1 (MQ=255)
                                  aCTTCGGTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTATTTCGCGAAGTCTAACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGAt                                                                                                                    <  2:3679209/149‑1 (MQ=255)
                                   cTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAATGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGAtt                                                                                                                   >  2:1656995/1‑149 (MQ=255)
                                         tGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATc                                                                                                             <  1:3564994/149‑1 (MQ=255)
                                            cTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCttt                                                                                                          <  2:4691908/149‑1 (MQ=255)
                                                cTCCCTGTCGACGAATATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTaa                                                                                                      <  1:2498477/149‑1 (MQ=255)
                                                 tCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAg                                                                                                     >  1:1602136/1‑149 (MQ=255)
                                                    cTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTAt                                                                                                  <  2:4517405/149‑1 (MQ=255)
                                                    cTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTAt                                                                                                  >  2:2136888/1‑149 (MQ=255)
                                                     tGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTAtt                                                                                                 >  2:1477043/1‑149 (MQ=255)
                                                      gTCGACGAAGAGAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTc                                                                                                <  2:3850178/149‑1 (MQ=255)
                                                        cgacgaAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGc                                                                                              <  1:2080053/149‑1 (MQ=255)
                                                         gacgaAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCt                                                                                             <  1:364548/149‑1 (MQ=255)
                                                           cgaAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTa                                                                                           <  2:1165686/149‑1 (MQ=255)
                                                            gaAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTAt                                                                                          <  2:2495058/149‑1 (MQ=255)
                                                             aaGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAAGCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTAtt                                                                                         <  1:1259357/149‑1 (MQ=255)
                                                               gATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTc                                                                                       <  1:2656336/149‑1 (MQ=255)
                                                                 tAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCt                                                                                     <  2:3777254/149‑1 (MQ=255)
                                                                  aGACTCGTCTTTTTCGCGAAGTCTTACACGGCGCTTACAGACAAGGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTg                                                                                    <  1:3006426/149‑1 (MQ=255)
                                                                  aGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTg                                                                                    <  2:642288/149‑1 (MQ=255)
                                                                  aGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTg                                                                                    >  2:3871756/1‑149 (MQ=255)
                                                                   gAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTgg                                                                                   <  2:2282637/149‑1 (MQ=255)
                                                                     aTCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCg                                                                                 >  2:4147297/1‑149 (MQ=255)
                                                                      tCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGa                                                                                <  2:547748/149‑1 (MQ=255)
                                                                         tCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAATGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGAtct                                                                             <  1:1813843/149‑1 (MQ=255)
                                                                         tCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGAtct                                                                             >  1:110642/1‑149 (MQ=255)
                                                                                 gcgAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACaga                                                                     >  2:2437013/1‑149 (MQ=255)
                                                                                     aGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCt                                                                 >  1:4092952/1‑149 (MQ=255)
                                                                                     aGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCt                                                                 >  1:4012349/1‑149 (MQ=255)
                                                                                       tCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGt                                                               >  1:331539/1‑149 (MQ=255)
                                                                                         ttACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTaa                                                             >  1:1761507/1‑149 (MQ=255)
                                                                                           aCAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAaga                                                           >  1:3261421/1‑149 (MQ=255)
                                                                                             aaGGCGCTTACACAAAATGCCATAAGGAAAATTTCATATTAAATTTAATATTTAAAAAAAAACATTTTTATTTTAATTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCGACAGAGTCTGTAAgaga                                                         <  1:1017985/149‑1 (MQ=255)
                                                                                              aGGCGCTTACCGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGAt                                                        <  1:1501723/149‑1 (MQ=255)
                                                                                                   cTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGAtctctc                                                   <  2:74363/149‑1 (MQ=255)
                                                                                                   cTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGAtctctc                                                   >  2:2027996/1‑149 (MQ=255)
                                                                                                   cTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAAAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGAtctctc                                                   >  1:4299728/1‑149 (MQ=255)
                                                                                                   cTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAAAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTAGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGAtctctc                                                   >  1:981817/1‑149 (MQ=255)
                                                                                                    ttACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTCAGAGATCTCTca                                                  >  2:3080113/1‑149 (MQ=255)
                                                                                                    ttACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTca                                                  >  1:1177983/1‑149 (MQ=255)
                                                                                                        aGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTACGAGATCTCTCACAAc                                              >  1:2238612/1‑149 (MQ=255)
                                                                                                        aGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAAc                                              >  1:4103536/1‑149 (MQ=255)
                                                                                                          aCAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCa                                            >  2:95535/1‑149 (MQ=255)
                                                                                                          aCAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCa                                            <  2:1081876/149‑1 (MQ=255)
                                                                                                            aaTGCCTTAAGTAAAATTTCATATGAAATTTAAGAATTAACAATCAACATTTTTATTTTAATTGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGa                                          <  1:3755205/149‑1 (MQ=255)
                                                                                                             aTGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGAt                                         >  1:3407261/1‑149 (MQ=255)
                                                                                                              tGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATg                                        <  1:2368689/149‑1 (MQ=255)
                                                                                                                ccTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATggg                                      <  1:1309549/149‑1 (MQ=255)
                                                                                                                 attaattaaaaTTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATgggg                                     <  1:2981561/142‑1 (MQ=255)
                                                                                                                   tAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTa                                   <  1:1849376/149‑1 (MQ=255)
                                                                                                                   tAAGGAAAATTTCATATGAAAGTTAAGAATTAACAAACAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTa                                   <  2:501786/149‑1 (MQ=255)
                                                                                                                    aaGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTACGAGATCTCTCACAACCAGATGGGGTAt                                  >  2:3546699/1‑149 (MQ=255)
                                                                                                                     aGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTAtt                                 >  1:4571806/1‑149 (MQ=255)
                                                                                                                       gAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTAGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGAGGGGGTATTTg                               >  2:3345615/1‑149 (MQ=255)
                                                                                                                        aaaaTTTCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTgg                              >  2:2188102/1‑149 (MQ=255)
                                                                                                                          aaTTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGAt                            <  1:4718173/149‑1 (MQ=255)
                                                                                                                           aTTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGAtt                           <  1:1455603/149‑1 (MQ=255)
                                                                                                                             ttCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                         >  1:2309463/1‑149 (MQ=255)
                                                                                                                             ttCATATGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                         <  1:1073011/149‑1 (MQ=255)
                                                                                                                             ttCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTAc                         >  2:1860999/1‑149 (MQ=255)
                                                                                                                                atatGAAAGTTAAGAATTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACagt                      >  2:3234531/1‑148 (MQ=255)
                                                                                                                                atatGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACaga                      >  2:2737589/1‑149 (MQ=255)
                                                                                                                                 tatGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTCTTTGGATTACagac                     >  1:4487960/1‑148 (MQ=255)
                                                                                                                                   tGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACagaaga                   <  2:2039925/149‑1 (MQ=255)
                                                                                                                                      aaGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGc                <  1:3149781/149‑1 (MQ=255)
                                                                                                                                          tAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTaa            >  2:3030142/1‑149 (MQ=255)
                                                                                                                                           aaGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAg           <  1:3796147/149‑1 (MQ=255)
                                                                                                                                             gAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGtt         <  2:2951148/149‑1 (MQ=255)
                                                                                                                                              aaTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTg        >  1:4332393/1‑149 (MQ=255)
                                                                                                                                              aaTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTg        >  1:2189761/1‑149 (MQ=255)
                                                                                                                                               aTTAATAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTACTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGATTAAGTTGa       >  2:3517486/1‑149 (MQ=255)
                                                                                                                                               aTTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGa       >  1:1440270/1‑149 (MQ=255)
                                                                                                                                                 tAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGAtt     <  1:2783478/149‑1 (MQ=255)
                                                                                                                                                   aCAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTaa   >  1:600422/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTACAAGGCGCTTACAGACAATGCCTTAAGGAAAATTTCATATGAAAGTTAAGAATTAACAATCAACATTTTGATTTTAAATGAAAATAACGATTCTTATCTTTTTAAGTATTCGCTTATTTCCTGGCGATCTCCTACAGAGTCTGTAAGAGATCTCTCACAACCAGATGGGGTATTTGGATTACAGAAGAGGCTTAAGTTGATTAAG  >  CP009273/2917824‑2918120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑