BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,918,415	G→A	84.7%	P75P (CCC→CCT)	syd ←	SecY‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,918,415	0	G	A	84.7%	768.0 / 130.2	302	P75P (CCC→CCT)	syd	SecY‑interacting protein
Reads supporting (aligned to +/- strand): ref base G (23/23); new base A (122/130); total (146/156)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

Only 100 of 306 total aligned reads displayed.

TTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918269‑2918559

ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTagg                                                                                                                                                <  1:3983265/149‑1 (MQ=255)

ttCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTagg                                                                                                                                                <  2:3890194/149‑1 (MQ=255)

 tCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGt                                                                                                                                               <  2:1393314/149‑1 (MQ=255)

   tGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTg                                                                                                                                             <  1:3723176/149‑1 (MQ=255)

    gAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGt                                                                                                                                            <  2:509574/149‑1 (MQ=255)

     aGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTa                                                                                                                                           >  1:75743/1‑149 (MQ=255)

           cAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACaa                                                                                                                                     >  1:575967/1‑149 (MQ=255)

            aGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAat                                                                                                                                    >  1:4060949/1‑149 (MQ=255)

             gATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAata                                                                                                                                   <  2:452023/149‑1 (MQ=255)

             gATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAata                                                                                                                                   <  2:4103541/149‑1 (MQ=255)

             gATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAata                                                                                                                                   <  1:3345605/149‑1 (MQ=255)

             gATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAata                                                                                                                                   <  1:4188574/149‑1 (MQ=255)

               tGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATc                                                                                                                                 <  2:164594/149‑1 (MQ=255)

                 aCCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaa                                                                                                                               >  2:830892/1‑149 (MQ=255)

                  ccAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaa                                                                                                                              <  2:4335127/149‑1 (MQ=255)

                   cAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaaa                                                                                                                             <  2:1313346/149‑1 (MQ=255)

                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCACTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAgc                                                                                                                           >  2:533106/1‑149 (MQ=255)

                     aTCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAgc                                                                                                                           <  1:2957091/149‑1 (MQ=255)

                           ttttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTc                                                                                                                     >  2:297506/1‑149 (MQ=255)

                             ttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCaa                                                                                                                   <  1:1415385/149‑1 (MQ=255)

                              tCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAAc                                                                                                                  >  1:3516657/1‑149 (MQ=255)

                               ccTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAAcc                                                                                                                 >  2:3977080/1‑149 (MQ=255)

                                   cACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             <  1:1726542/149‑1 (MQ=255)

                                   cACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGt                                                                                                             <  2:325827/149‑1 (MQ=255)

                                    aCCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGtt                                                                                                            <  2:2052435/149‑1 (MQ=255)

                                     cccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           >  1:673036/1‑149 (MQ=255)

                                     cccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           >  1:2014848/1‑149 (MQ=255)

                                         aCGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACag                                                                                                       >  2:3069480/1‑148 (MQ=255)

                                         aCGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAt                                                                                                       >  2:1083860/1‑149 (MQ=255)

                                          cGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAtt                                                                                                      <  2:4463997/149‑1 (MQ=255)

                                           ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAttt                                                                                                     >  1:37894/1‑149 (MQ=255)

                                           ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAttt                                                                                                     >  1:4412646/1‑149 (MQ=255)

                                           ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAttt                                                                                                     >  1:2885629/1‑149 (MQ=255)

                                            gAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAtttt                                                                                                    <  2:2165559/149‑1 (MQ=255)

                                            gAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGGATAAAAGGTATGAATTGTAGGTTGTATCACAAGATCAAAAGCGCGTTCCACCGCGGTGACAtggt                                                                                                    >  2:3429511/1‑146 (MQ=255)

                                               gtcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGtt                                                                                                 <  2:2303331/149‑1 (MQ=255)

                                               gtcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGtt                                                                                                 <  1:2468080/149‑1 (MQ=255)

                                                tcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTc                                                                                                >  1:3004710/1‑149 (MQ=255)

                                                tcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTc                                                                                                <  1:531864/149‑1 (MQ=255)

                                                  gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTccc                                                                                              >  2:2859229/1‑149 (MQ=255)

                                                   tcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTcccc                                                                                             <  1:4332037/149‑1 (MQ=255)

                                                     ttCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCgg                                                                                           >  1:1029114/1‑149 (MQ=255)

                                                      tCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCACACTGAGTCGTATAAAAGGTCTGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAGTGTGTTCCCCGGt                                                                                          >  2:4300400/1‑149 (MQ=255)

                                                       cACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTa                                                                                         >  2:1270697/1‑149 (MQ=255)

                                                       cACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTa                                                                                         <  1:2622012/149‑1 (MQ=255)

                                                        aCTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTaa                                                                                        >  1:4389204/1‑149 (MQ=255)

                                                          tCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAAc                                                                                      <  1:2139999/149‑1 (MQ=255)

                                                           ccAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACg                                                                                     <  1:2556882/149‑1 (MQ=255)

                                                            cAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACgg                                                                                    <  2:4509158/149‑1 (MQ=255)

                                                             aGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGc                                                                                   >  2:664327/1‑149 (MQ=255)

                                                             aGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGc                                                                                   <  1:3294535/149‑1 (MQ=255)

                                                              ggTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCt                                                                                  >  1:1656464/1‑149 (MQ=255)

                                                               gTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTg                                                                                 <  1:1476344/149‑1 (MQ=255)

                                                               gTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTg                                                                                 <  2:4244550/149‑1 (MQ=255)

                                                                tttGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTgg                                                                                <  2:1163143/149‑1 (MQ=255)

                                                                  tGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTgggg                                                                              >  1:1927869/1‑149 (MQ=255)

                                                                      gcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGc                                                                          <  2:1304067/149‑1 (MQ=255)

                                                                           gTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGACTTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGta                                                                     <  1:3655166/149‑1 (MQ=255)

                                                                            tCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGtat                                                                    >  1:1857464/1‑149 (MQ=255)

                                                                              aGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATAc                                                                  <  2:3578781/149‑1 (MQ=255)

                                                                               gTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACg                                                                 >  1:2243109/1‑149 (MQ=255)

                                                                               gTTTGATATCGCCAAACTGCGCGTGCATATCCCCGACAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACg                                                                 >  1:3694741/1‑149 (MQ=255)

                                                                                     tatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCt                                                           >  1:1531882/1‑149 (MQ=255)

                                                                                      atCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCtt                                                          <  2:3323489/149‑1 (MQ=255)

                                                                                      atCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCtt                                                          >  2:4633719/1‑149 (MQ=255)

                                                                                       tCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTc                                                         >  1:1134132/1‑149 (MQ=255)

                                                                                         gCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg                                                       >  1:3214733/1‑149 (MQ=255)

                                                                                         gCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg                                                       <  2:493514/149‑1 (MQ=255)

                                                                                            aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAg                                                    <  2:253093/149‑1 (MQ=255)

                                                                                            aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAg                                                    >  2:3870250/1‑149 (MQ=255)

                                                                                               cTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCGGGGGTTGCCAGTATACGGCATCTTCGGTAGTTg                                                 >  2:1540328/1‑149 (MQ=255)

                                                                                                tGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGa                                                >  1:3386093/1‑149 (MQ=255)

                                                                                                  cgcgTGCATATCCCCGGCAAACTGAGTCTTATAAAAGGTATGATTTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGaaa                                              <  1:2820787/149‑1 (MQ=255)

                                                                                                   gcgTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaat                                             <  2:4186804/149‑1 (MQ=255)

                                                                                                        cATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGc                                        <  1:4557186/149‑1 (MQ=255)

                                                                                                         atatCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCa                                       <  2:1474608/149‑1 (MQ=255)

                                                                                                          tatCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCAc                                      <  2:3543836/149‑1 (MQ=255)

                                                                                                              cccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTg                                  <  1:1564662/149‑1 (MQ=255)

                                                                                                               ccGGCAAACTGAGTCTTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGa                                 <  1:1554077/149‑1 (MQ=255)

                                                                                                                  gCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAgg                              <  1:4340134/149‑1 (MQ=255)

                                                                                                                   cacaCTGAGGCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTACACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGa                             <  2:4084955/146‑1 (MQ=255)

                                                                                                                     aaCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAac                           >  2:1940578/1‑149 (MQ=255)

                                                                                                                      aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAaca                          >  1:1200130/1‑149 (MQ=255)

                                                                                                                      aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAaca                          <  1:4226961/149‑1 (MQ=255)

                                                                                                                         gAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCg                       >  1:2045125/1‑149 (MQ=255)

                                                                                                                          aGTCGTATAAAAGGTATGAATTGTCGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTACACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGt                      <  1:824234/149‑1 (MQ=255)

                                                                                                                            tCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTAc                    >  1:48010/1‑149 (MQ=255)

                                                                                                                            tCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTAc                    >  2:2994042/1‑149 (MQ=255)

                                                                                                                              gTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAg                  >  2:212759/1‑149 (MQ=255)

                                                                                                                                ataAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCGGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGtt                <  1:126641/149‑1 (MQ=255)

                                                                                                                                 taAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTc               >  2:1071973/1‑149 (MQ=255)

                                                                                                                                  aaaaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTcc              <  1:3898362/149‑1 (MQ=255)

                                                                                                                                   aaaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTTTTCCCCGTTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCt             <  1:3977080/149‑1 (MQ=255)

                                                                                                                                     aGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           <  1:4254387/149‑1 (MQ=255)

                                                                                                                                     aGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           <  2:3698506/149‑1 (MQ=255)

                                                                                                                                      ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAc          >  1:3477542/1‑149 (MQ=255)

                                                                                                                                      ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAc          <  1:72852/149‑1 (MQ=255)

                                                                                                                                      ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCAc          >  2:4158409/1‑149 (MQ=255)

                                                                                                                                        tATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACtt        <  2:612530/149‑1 (MQ=255)

                                                                                                                                             tttGTGGGTTGTATAACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACgg   <  1:1613289/148‑1 (MQ=255)

TTCTGAGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918269‑2918559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`