Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,921,101 C→A 85.9% intergenic (+70/‑487) ygdH → / → sdaC UPF0717 family protein/putative serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,921,1010CA85.9% 554.1 / 98.3 238intergenic (+70/‑487)ygdH/sdaCUPF0717 family protein/putative serine transporter
Reads supporting (aligned to +/- strand):  ref base C (14/20);  new base A (95/109);  total (109/129)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 261 total aligned reads displayed.

CTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATT  >  CP009273/2920954‑2921248
                                                                                                                                                   |                                                                                                                                                   
 tACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTGCATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcatttaat                                                                                                                                                   >  1:1942311/1‑149 (MQ=255)
 tACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcatttaat                                                                                                                                                   >  1:3443359/1‑149 (MQ=255)
   cAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTcatttaattt                                                                                                                                                 >  2:2372358/1‑149 (MQ=255)
    aGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTg                                                                                                                                                >  1:1210915/1‑149 (MQ=255)
      ggTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGtt                                                                                                                                              <  1:4372859/149‑1 (MQ=255)
       gTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTta                                                                                                                                             >  1:2991410/1‑149 (MQ=255)
       gTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTta                                                                                                                                             >  1:2126200/1‑149 (MQ=255)
       gTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTta                                                                                                                                             <  2:210868/149‑1 (MQ=255)
        ttttGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTtat                                                                                                                                            >  2:4375243/1‑149 (MQ=255)
        ttttGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTtat                                                                                                                                            <  2:3836791/149‑1 (MQ=255)
             ttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGaa                                                                                                                                       <  2:3529563/149‑1 (MQ=255)
               gCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATg                                                                                                                                     <  2:1726851/149‑1 (MQ=255)
               gCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATg                                                                                                                                     <  1:3826089/149‑1 (MQ=255)
                 ccAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGtt                                                                                                                                   <  1:568886/149‑1 (MQ=255)
                   aGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTc                                                                                                                                 >  2:1925139/1‑149 (MQ=255)
                   aGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTc                                                                                                                                 <  1:3688906/149‑1 (MQ=255)
                      aTCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTa                                                                                                                              <  2:1272400/149‑1 (MQ=255)
                      aTCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTa                                                                                                                              >  1:1049241/1‑149 (MQ=255)
                           aTGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTaccacc                                                                                                                         >  1:2760516/1‑149 (MQ=255)
                            tGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCAccc                                                                                                                        >  1:2959139/1‑149 (MQ=255)
                            tGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCAccc                                                                                                                        >  1:1052109/1‑149 (MQ=255)
                            tGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGAACGTAATATCCTCTCCCCCGGTGACGTTTGTTGCCTGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTTTTATATGAATGTTTCTTACCAccc                                                                                                                        <  1:1680499/149‑1 (MQ=255)
                               aGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCa                                                                                                                     <  2:69604/149‑1 (MQ=255)
                                gTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCAc                                                                                                                    >  2:1226990/1‑149 (MQ=255)
                                      aGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACa                                                                                                              >  1:2446047/1‑149 (MQ=255)
                                      aGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACa                                                                                                              >  1:572887/1‑149 (MQ=255)
                                            aGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatc                                                                                                        >  1:86628/1‑149 (MQ=255)
                                             gCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatca                                                                                                       >  1:3164441/1‑149 (MQ=255)
                                              ccTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcat                                                                                                      >  2:1624159/1‑149 (MQ=255)
                                              ccTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcat                                                                                                      >  1:3091870/1‑149 (MQ=255)
                                                   aTCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAg                                                                                                 <  1:1259043/149‑1 (MQ=255)
                                                   aTCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAg                                                                                                 >  1:334318/1‑149 (MQ=255)
                                                      ccTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTa                                                                                              <  2:3746403/149‑1 (MQ=255)
                                                      ccTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTa                                                                                              <  2:4039247/149‑1 (MQ=255)
                                                        ttGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                                                            <  1:3498258/149‑1 (MQ=255)
                                                          gCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAatat                                                                                          <  1:3227801/149‑1 (MQ=255)
                                                          gCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCAGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAatat                                                                                          <  2:627439/149‑1 (MQ=255)
                                                              cGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCa                                                                                      <  1:385753/149‑1 (MQ=255)
                                                                aaaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATa                                                                                    >  1:1366528/1‑149 (MQ=255)
                                                                 aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATAc                                                                                   <  2:1531523/149‑1 (MQ=255)
                                                                     tGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAAtt                                                                               <  1:3982379/149‑1 (MQ=255)
                                                                           tAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGtt                                                                         <  1:2734278/149‑1 (MQ=255)
                                                                            aaTCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTg                                                                        <  1:1593635/149‑1 (MQ=255)
                                                                             aTCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGc                                                                       <  1:2661467/149‑1 (MQ=255)
                                                                              tctcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATAATCACAGTTAATATTTCATAAAATTTATTTTTCa                                                                      >  1:4094933/1‑149 (MQ=255)
                                                                              tctcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGGGTTTGGTCACGTACGCATCCACTCCGTTCAGTTGTTATATGACGGTTTCTTACCCCCCTCACGCGACCAATAGCAGCACAGTTACGATGTCATACAATTGATGTTGCa                                                                      >  2:283148/1‑149 (MQ=255)
                                                                               ctcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCaa                                                                     >  2:4273646/1‑149 (MQ=255)
                                                                                tcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAAc                                                                    >  2:349451/1‑149 (MQ=255)
                                                                                tcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAAc                                                                    >  2:3406712/1‑149 (MQ=255)
                                                                                tcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAAc                                                                    >  2:1195275/1‑149 (MQ=255)
                                                                                    cTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCaa                                                                <  1:4773813/149‑1 (MQ=255)
                                                                                     tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCaaa                                                               >  1:1820482/1‑149 (MQ=255)
                                                                                      cccccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAAc                                                              <  1:1090927/149‑1 (MQ=255)
                                                                                        cccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGt                                                            <  2:3719861/149‑1 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGtt                                                           <  1:2327711/149‑1 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGtt                                                           <  1:2923438/149‑1 (MQ=255)
                                                                                            gTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTcc                                                        >  2:3987240/1‑149 (MQ=255)
                                                                                            gTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTcc                                                        >  2:3901513/1‑149 (MQ=255)
                                                                                                  tttGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTAttt                                                  <  2:4089728/149‑1 (MQ=255)
                                                                                                  tttGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATGTGTTAGATGAATGTTTCTTACCACCCTCACGCGACACATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTAttt                                                  <  2:4502093/149‑1 (MQ=255)
                                                                                                          cGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAAc                                          <  2:289845/149‑1 (MQ=255)
                                                                                                          cGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAAc                                          >  1:2975994/1‑149 (MQ=255)
                                                                                                           gggTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAAcc                                         <  2:4438189/149‑1 (MQ=255)
                                                                                                           gggTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAAcc                                         <  1:990000/149‑1 (MQ=255)
                                                                                                             attttCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTTTGTTGCAACGAAAACGTTTCCCTATTTTCATAAACCGt                                       <  1:1798628/148‑1 (MQ=255)
                                                                                                              ttttCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTa                                      >  2:835489/1‑149 (MQ=255)
                                                                                                               tttCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAt                                     <  1:2852027/149‑1 (MQ=255)
                                                                                                                ttCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAtt                                    <  1:4422843/149‑1 (MQ=255)
                                                                                                                ttCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAtt                                    <  2:3091870/149‑1 (MQ=255)
                                                                                                                ttCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAtt                                    <  2:2991410/149‑1 (MQ=255)
                                                                                                                ttCTCGTTTTTGGTCAATTACTCATCAACTCATTTAATTTTTTATATGAATGTTTCTTACCACCCTCACGCTACAAATATAATCAAAGTTAATATGTCATACAATTTATGTTTCAACTCAAACGTTTCCCTATTTTCATAAACCGTAtt                                    <  1:219068/149‑1 (MQ=255)
                                                                                                                 tctcGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAttt                                   <  1:682344/149‑1 (MQ=255)
                                                                                                                  ctcGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAtttt                                  <  2:2373081/149‑1 (MQ=255)
                                                                                                                     gTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATc                               <  1:1629167/149‑1 (MQ=255)
                                                                                                                      tttttGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCg                              >  1:2415236/1‑149 (MQ=255)
                                                                                                                       ttttGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGc                             <  2:1049241/149‑1 (MQ=255)
                                                                                                                        tttGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCt                            >  2:1013691/1‑149 (MQ=255)
                                                                                                                          tGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTAt                          <  2:47927/149‑1 (MQ=255)
                                                                                                                            gTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGa                        >  2:3037923/1‑149 (MQ=255)
                                                                                                                             tCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGaa                       <  1:4734517/149‑1 (MQ=255)
                                                                                                                                  tACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGa                  <  2:1942311/149‑1 (MQ=255)
                                                                                                                                   aCTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGTTATGAAAAAGaa                 >  1:2481116/1‑149 (MQ=255)
                                                                                                                                   aCTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTCATCGTTATGAAAAAGaa                 >  1:490320/1‑149 (MQ=255)
                                                                                                                                     tcatcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAAt               >  1:3535481/1‑149 (MQ=255)
                                                                                                                                      catcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAAtt              >  2:1311055/1‑149 (MQ=255)
                                                                                                                                        tcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTAt            <  2:3858770/149‑1 (MQ=255)
                                                                                                                                        tcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTAt            <  2:960448/149‑1 (MQ=255)
                                                                                                                                        tcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTAt            >  1:2290357/1‑149 (MQ=255)
                                                                                                                                        tcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTAt            <  2:2519292/149‑1 (MQ=255)
                                                                                                                                        tcaACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGAAACTCAAACGTTTACCTATTTTCATAAACAGTATTTTATCTCTATTAAAAAGAAATTAt            >  1:1311622/1‑149 (MQ=255)
                                                                                                                                         caACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATc           <  1:1434813/149‑1 (MQ=255)
                                                                                                                                         caACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATc           <  1:1434749/149‑1 (MQ=255)
                                                                                                                                           aCTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGc         <  2:4766143/149‑1 (MQ=255)
                                                                                                                                            cTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGcc        >  1:3564383/1‑149 (MQ=255)
                                                                                                                                            cTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGcc        >  2:3244037/1‑149 (MQ=255)
                                                                                                                                             tcatttaattTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCa       >  1:3318605/1‑149 (MQ=255)
                                                                                                                                               atttcatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATg     >  1:3420710/1‑149 (MQ=255)
                                                                                                                                               atttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCAtt     >  2:4564576/1‑148 (MQ=255)
                                                                                                                                                tttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCAtta    >  1:3787172/1‑147 (MQ=255)
                                                                                                                                                  tcatttGTTATATGAATGTTTCTTACCACCCTCACGCGACACATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGAtt  <  2:1397849/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATT  >  CP009273/2920954‑2921248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑