Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,932,835 C→T 100% R37C (CGC→TGC)  fucR → l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,932,8350CT98.9% 972.0 / ‑0.6 281R37C (CGC→TGC) fucRl‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (120/158);  total (122/159)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 284 total aligned reads displayed.

CATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGC  >  CP009273/2932689‑2932979
                                                                                                                                                  |                                                                                                                                                
  tGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGa                                                                                                                                              <  2:3916888/149‑1 (MQ=255)
        gggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCaa                                                                                                                                        <  1:4420741/149‑1 (MQ=255)
          gcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATg                                                                                                                                      >  2:2073426/1‑149 (MQ=255)
            ggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGaa                                                                                                                                    >  1:1584870/1‑149 (MQ=255)
            ggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGaa                                                                                                                                    <  1:448151/149‑1 (MQ=255)
                  cGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAg                                                                                                                              >  2:4234424/1‑149 (MQ=255)
                     atgatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACg                                                                                                                           >  2:46708/1‑149 (MQ=255)
                     atgatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACg                                                                                                                           >  2:2440432/1‑149 (MQ=255)
                      tgatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGc                                                                                                                          <  2:3639303/149‑1 (MQ=255)
                       gatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCa                                                                                                                         <  1:2401002/149‑1 (MQ=255)
                       gatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCa                                                                                                                         >  1:4092428/1‑149 (MQ=255)
                        atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                        <  1:3820557/149‑1 (MQ=255)
                        atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                        <  2:1609970/149‑1 (MQ=255)
                        atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                        <  1:3003731/149‑1 (MQ=255)
                        atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                        <  1:3394462/149‑1 (MQ=255)
                         tgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAgg                                                                                                                       >  2:2841164/1‑149 (MQ=255)
                          gggtTGAAAATTTTGAATGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCGGTCCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACAATTCGTTGCGATCTCAATGAATTACAGACGCAggg                                                                                                                      <  1:753396/149‑1 (MQ=255)
                          ggggTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAggg                                                                                                                      >  2:1559806/1‑149 (MQ=255)
                           gggTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGt                                                                                                                     <  2:514798/149‑1 (MQ=255)
                             gTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGACAGAAAAGAAAACATTCGTTTCGATCTCAATGAATTACAGACGCAGGGTaa                                                                                                                   >  1:2598043/1‑149 (MQ=255)
                              tGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaaa                                                                                                                  >  1:3541420/1‑149 (MQ=255)
                               gAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaaaa                                                                                                                 <  1:2136721/149‑1 (MQ=255)
                               gAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaaaa                                                                                                                 >  1:3782996/1‑149 (MQ=255)
                                aaaaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAt                                                                                                                <  2:660920/149‑1 (MQ=255)
                                 aaaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAtt                                                                                                               <  1:1787681/149‑1 (MQ=255)
                                   aaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCt                                                                                                             <  2:4024904/149‑1 (MQ=255)
                                    atatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTg                                                                                                            >  2:4770450/1‑149 (MQ=255)
                                     tatGAAAGCGGCACGCCAGCAAGCGATATTCGACCTGCTGCTGAACCATACAAGCCTGACCACGGAAGCTCTATCTGAACAGCTAAAGGTCAGTAAAGAAACCTTTCGTTGCGTTCTCAATGAATTACAGACGCAGGGTAAAATTCTgc                                                                                                           <  2:383005/149‑1 (MQ=255)
                                      atGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTCAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgcg                                                                                                          >  2:2794300/1‑149 (MQ=255)
                                            gCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCa                                                                                                    <  1:748589/149‑1 (MQ=255)
                                            gCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCa                                                                                                    >  1:3964368/1‑149 (MQ=255)
                                                      aGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCt                                                                                          >  1:1949877/1‑149 (MQ=255)
                                                       gCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCtc                                                                                         >  1:578692/1‑148 (MQ=255)
                                                       gCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTa                                                                                         >  1:2708647/1‑149 (MQ=255)
                                                           gCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGATGCGCTAAata                                                                                     >  2:1036284/1‑149 (MQ=255)
                                                             gATAGTCGACCTGCTGCTGAACCAGACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAatata                                                                                   <  2:1436539/149‑1 (MQ=255)
                                                                aGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATcc                                                                                <  1:3059056/149‑1 (MQ=255)
                                                                 gTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCa                                                                               >  2:2232699/1‑149 (MQ=255)
                                                                   cGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCAcc                                                                             <  1:3387360/149‑1 (MQ=255)
                                                                    gACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCGAAATATATCCACCg                                                                            >  2:4065470/1‑149 (MQ=255)
                                                                     aCCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGt                                                                           <  2:1522384/149‑1 (MQ=255)
                                                                     aCCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGt                                                                           <  2:1522555/149‑1 (MQ=255)
                                                                      ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTc                                                                          <  1:942182/149‑1 (MQ=255)
                                                                      ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTc                                                                          <  1:1308885/149‑1 (MQ=255)
                                                                       ctgctgctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCa                                                                         <  1:1401734/149‑1 (MQ=255)
                                                                        tgctgctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCaa                                                                        >  1:3658449/1‑149 (MQ=255)
                                                                                aaCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGa                                                                <  1:3311460/149‑1 (MQ=255)
                                                                                 aCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGAc                                                               <  2:167072/149‑1 (MQ=255)
                                                                                  ccATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACa                                                              <  1:3226190/149‑1 (MQ=255)
                                                                                    aTACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGt                                                            <  2:3550948/149‑1 (MQ=255)
                                                                                       ccAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGc                                                         <  1:4143740/149‑1 (MQ=255)
                                                                                         aGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGa                                                       <  1:1938072/149‑1 (MQ=255)
                                                                                            cTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATcc                                                    >  1:1212251/1‑149 (MQ=255)
                                                                                              gACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCt                                                  >  1:109845/1‑149 (MQ=255)
                                                                                              gACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCt                                                  <  2:253664/149‑1 (MQ=255)
                                                                                                 cACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTc                                               <  2:1979595/149‑1 (MQ=255)
                                                                                                  aCGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTca                                              >  2:4619770/1‑149 (MQ=255)
                                                                                                  aCGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTca                                              <  1:4614016/149‑1 (MQ=255)
                                                                                                    ggAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTcaca                                            <  1:4270879/149‑1 (MQ=255)
                                                                                                     gAAGCTCTCTCTGAACAGCTAAAGGTGAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACAt                                           >  2:1185826/1‑149 (MQ=255)
                                                                                                     gAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACAt                                           >  1:2046141/1‑149 (MQ=255)
                                                                                                     gAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACAt                                           <  2:3174342/149‑1 (MQ=255)
                                                                                                     gAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACAt                                           <  1:2841164/149‑1 (MQ=255)
                                                                                                       aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCa                                         >  2:941284/1‑149 (MQ=255)
                                                                                                       aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCa                                         <  1:4186023/149‑1 (MQ=255)
                                                                                                            tctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                    <  2:715611/149‑1 (MQ=255)
                                                                                                            tctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                    <  1:150018/149‑1 (MQ=255)
                                                                                                            tctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                    >  1:4645188/1‑149 (MQ=255)
                                                                                                            tctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                    <  1:2778180/149‑1 (MQ=255)
                                                                                                            tctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                    <  2:376183/149‑1 (MQ=255)
                                                                                                             ctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGa                                   <  2:3390735/149‑1 (MQ=255)
                                                                                                              tctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATCTCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaa                                  >  2:3709166/1‑149 (MQ=255)
                                                                                                              tctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaa                                  >  2:1320037/1‑149 (MQ=255)
                                                                                                              tctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaa                                  >  2:2673503/1‑149 (MQ=255)
                                                                                                                tGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGaaaa                                >  1:1708046/1‑149 (MQ=255)
                                                                                                                    cAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCa                            <  1:3483266/149‑1 (MQ=255)
                                                                                                                     aGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAt                           <  2:311410/149‑1 (MQ=255)
                                                                                                                     aGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAt                           <  1:4760905/149‑1 (MQ=255)
                                                                                                                       cTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTa                         >  2:533748/1‑149 (MQ=255)
                                                                                                                        tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTAt                        <  1:2491320/149‑1 (MQ=255)
                                                                                                                        tAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTAt                        >  2:2333697/1‑149 (MQ=255)
                                                                                                                           aGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgcg                     >  2:4517887/1‑149 (MQ=255)
                                                                                                                           aGGTAAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgcg                     >  2:1841625/1‑149 (MQ=255)
                                                                                                                            ggTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgcgc                    <  1:2496561/149‑1 (MQ=255)
                                                                                                                             gTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCa                   >  2:4399515/1‑149 (MQ=255)
                                                                                                                               cAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATa                 <  1:100239/149‑1 (MQ=255)
                                                                                                                                   aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGc             >  2:4102395/1‑149 (MQ=255)
                                                                                                                                   aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGc             >  1:708243/1‑149 (MQ=255)
                                                                                                                                   aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAGTCTGCGCATAAAGc             >  1:2194872/1‑149 (MQ=255)
                                                                                                                                    aaGAAACCATTCGTTGCGATCTCCATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCa            <  1:1544711/149‑1 (MQ=255)
                                                                                                                                    aaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCa            <  2:3249510/149‑1 (MQ=255)
                                                                                                                                    aaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCa            >  1:1374818/1‑149 (MQ=255)
                                                                                                                                     aGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg           >  2:4334408/1‑149 (MQ=255)
                                                                                                                                      gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          <  2:3125273/149‑1 (MQ=255)
                                                                                                                                      gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa          >  1:2305695/1‑149 (MQ=255)
                                                                                                                                        aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        >  2:2703088/1‑149 (MQ=255)
                                                                                                                                        aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata        <  1:4591252/149‑1 (MQ=255)
                                                                                                                                          ccATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATc      <  1:2934806/149‑1 (MQ=255)
                                                                                                                                            aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgc    >  2:2923207/1‑149 (MQ=255)
                                                                                                                                            aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgc    <  2:622254/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGC  >  CP009273/2932689‑2932979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑