Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,934,843 G→T 100% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,934,8430GT100.0% 1044.5 / ‑6.7 305H50N (CAT→AAT) ygdDUPF0382 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (164/140);  minor base A (0/1);  total (164/141)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 305 total aligned reads displayed.

CACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTC  >  CP009273/2934698‑2934988
                                                                                                                                                 |                                                                                                                                                 
cacaAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTaaa                                                                                                                                                <  2:4388035/149‑1 (MQ=255)
 acaAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAAc                                                                                                                                               <  1:1105108/149‑1 (MQ=255)
   aaaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGc                                                                                                                                             >  2:4114847/1‑149 (MQ=255)
   aaaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGc                                                                                                                                             >  2:1725112/1‑149 (MQ=255)
      cGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTg                                                                                                                                          >  1:957102/1‑149 (MQ=255)
           aTGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTAtt                                                                                                                                     <  2:3862914/149‑1 (MQ=255)
               gACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAg                                                                                                                                 <  2:3347061/149‑1 (MQ=255)
                aCAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAgg                                                                                                                                <  2:2943184/149‑1 (MQ=255)
                 cAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAgtc                                                                                                                               >  1:105868/1‑147 (MQ=255)
                 cAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGc                                                                                                                               >  2:2284049/1‑149 (MQ=255)
                   gcgcCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCg                                                                                                                             >  1:901748/1‑149 (MQ=255)
                   gcgcCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCg                                                                                                                             >  1:2687727/1‑149 (MQ=255)
                     gcCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGt                                                                                                                           >  2:4079964/1‑149 (MQ=255)
                         ggCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTgg                                                                                                                       >  2:883399/1‑149 (MQ=255)
                            aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATc                                                                                                                    <  2:2704494/149‑1 (MQ=255)
                            aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATc                                                                                                                    <  2:2170323/149‑1 (MQ=255)
                             aTAACGGCTGCCGCTGAACAACACCGTGCCTACCGCGCGGAAAACGCTACGCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                   <  1:4495727/149‑1 (MQ=255)
                              tAACGGCTGCCGCTTAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCa                                                                                                                  <  1:824980/149‑1 (MQ=255)
                               aaaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAg                                                                                                                 >  2:1645276/1‑149 (MQ=255)
                               aaaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAg                                                                                                                 >  2:1278063/1‑149 (MQ=255)
                                aaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGTAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGc                                                                                                                <  1:2157364/149‑1 (MQ=255)
                                aaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGc                                                                                                                <  1:1057282/149‑1 (MQ=255)
                                aaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGc                                                                                                                <  1:2872766/149‑1 (MQ=255)
                                  ggCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGccc                                                                                                              >  2:4471996/1‑149 (MQ=255)
                                         gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCaa                                                                                                       <  1:1593596/149‑1 (MQ=255)
                                             aacaacACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGc                                                                                                   <  2:1993974/149‑1 (MQ=255)
                                                   acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCAt                                                                                             >  2:1155310/1‑149 (MQ=255)
                                                   acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCAt                                                                                             >  2:4319097/1‑149 (MQ=255)
                                                     cGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATgg                                                                                           <  2:3987543/149‑1 (MQ=255)
                                                        gCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGttt                                                                                        <  1:4223720/149‑1 (MQ=255)
                                                          cTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTa                                                                                      <  1:3889649/149‑1 (MQ=255)
                                                           tAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTAc                                                                                     >  1:2201481/1‑149 (MQ=255)
                                                            aaCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCTCCATGGTTTTACt                                                                                    >  1:3005733/1‑149 (MQ=255)
                                                            aaCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACt                                                                                    <  2:393418/149‑1 (MQ=255)
                                                            aaCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACt                                                                                    >  2:1370782/1‑149 (MQ=255)
                                                             aCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACtt                                                                                   <  1:4680058/149‑1 (MQ=255)
                                                               gcgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTaa                                                                                 >  1:2460200/1‑149 (MQ=255)
                                                                cgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAac                                                                                <  1:1422394/149‑1 (MQ=255)
                                                                 gAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAaca                                                                               <  2:3212276/149‑1 (MQ=255)
                                                                 gAGGAAAACGCTACTCCAGTAAAACCAGAGACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAaca                                                                               <  2:4247941/149‑1 (MQ=255)
                                                                  aGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAacac                                                                              >  1:3793679/1‑149 (MQ=255)
                                                                    gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACAt                                                                            >  1:130380/1‑149 (MQ=255)
                                                                     aaaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                           <  2:1996200/149‑1 (MQ=255)
                                                                      aaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgc                                                                          >  1:2260912/1‑149 (MQ=255)
                                                                       aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcg                                                                         <  2:3314258/149‑1 (MQ=255)
                                                                       aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcg                                                                         >  1:1711926/1‑149 (MQ=255)
                                                                        aCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                        >  1:254530/1‑149 (MQ=255)
                                                                        aCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                        >  2:2430416/1‑149 (MQ=255)
                                                                              cTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCaaa                                                                  >  2:30637/1‑149 (MQ=255)
                                                                               tCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCaaaa                                                                 >  1:4722588/1‑149 (MQ=255)
                                                                                ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAg                                                                <  1:4411650/149‑1 (MQ=255)
                                                                                 cAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCAGGGTTGTACTTAACACATGCGCGCCAAAAgc                                                               >  2:452555/1‑149 (MQ=255)
                                                                                  aGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgcg                                                              >  1:2625416/1‑149 (MQ=255)
                                                                                   gTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgcgc                                                             >  1:2829428/1‑149 (MQ=255)
                                                                                    tAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGcc                                                            >  1:333955/1‑149 (MQ=255)
                                                                                     aaaaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGccc                                                           <  1:180793/149‑1 (MQ=255)
                                                                                     aaaaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGccc                                                           >  2:2848685/1‑149 (MQ=255)
                                                                                        aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCC‑AAAGCGCCCagag                                                       >  2:2621907/1‑149 (MQ=255)
                                                                                         ccAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTATACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                       >  2:1168218/1‑149 (MQ=255)
                                                                                         ccAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCcgag                                                       >  2:1338686/1‑149 (MQ=255)
                                                                                         ccAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                       >  1:729635/1‑149 (MQ=255)
                                                                                            gATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCca                                                    >  2:1464910/1‑149 (MQ=255)
                                                                                              tACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                  >  1:3277599/1‑149 (MQ=255)
                                                                                              tACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                  >  1:4693251/1‑149 (MQ=255)
                                                                                                  gATGCGACGCGGCATGGCAACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaaaa                                              <  1:11952/149‑1 (MQ=255)
                                                                                                   aTGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAAt                                             <  2:4744209/149‑1 (MQ=255)
                                                                                                     gCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGa                                           >  2:2992966/1‑149 (MQ=255)
                                                                                                      cGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGaa                                          <  2:792542/149‑1 (MQ=255)
                                                                                                      cGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGaa                                          <  1:4598131/149‑1 (MQ=255)
                                                                                                       gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAg                                         <  2:3550462/149‑1 (MQ=255)
                                                                                                        aCGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                        <  1:3717393/149‑1 (MQ=255)
                                                                                                         cGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGcc                                       <  1:250131/149‑1 (MQ=255)
                                                                                                          gCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCg                                      >  1:3146725/1‑149 (MQ=255)
                                                                                                          gCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCg                                      >  2:2501222/1‑149 (MQ=255)
                                                                                                          gCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCg                                      >  2:153965/1‑149 (MQ=255)
                                                                                                           ctgatttGCCACCGCAAGACCTAAGATCGCCAGCGTTTTAAACGCCTGGTATTCGTGGCGGGTCTGTATCCAGCCCATCTCAACGGCCCCCTTGGTTTTACTTAACACATGCGCGCAAAAAGCGCCCAGAGCCACAAAAATGAAGCCGc                                     <  1:1652881/144‑1 (MQ=255)
                                                                                                           cTGAATTGCCACCGCCTGACCTAAGATCGCCTGCGTATTAAACGCCTGGTATTCGAGGCGGGTCTGTATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGc                                     <  1:1397056/149‑1 (MQ=255)
                                                                                                            tGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCt                                    <  2:3099977/149‑1 (MQ=255)
                                                                                                            tGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCt                                    >  2:422250/1‑149 (MQ=255)
                                                                                                              cATTGCCACCGCCAGACCTAAGATCGCCAGCTTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTaa                                  <  1:1358476/149‑1 (MQ=255)
                                                                                                                ttGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATg                                >  2:480979/1‑149 (MQ=255)
                                                                                                                 tGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTAAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATgg                               <  1:203278/149‑1 (MQ=255)
                                                                                                                  gCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggc                              <  2:2351046/149‑1 (MQ=255)
                                                                                                                  gCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggc                              >  2:3599278/1‑149 (MQ=255)
                                                                                                                          cAGACCTAAGATCGCCAGCGTATTAAACGCGTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGTTTTTACTTAACACATGCGCGCCACAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaaa                      <  2:2034679/149‑1 (MQ=255)
                                                                                                                           aGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAAt                     >  1:450984/1‑149 (MQ=255)
                                                                                                                            gACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATc                    >  1:4030068/1‑149 (MQ=255)
                                                                                                                            gACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATc                    <  2:1058116/149‑1 (MQ=255)
                                                                                                                               cTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGc                 >  1:1985706/1‑149 (MQ=255)
                                                                                                                               cTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGc                 <  2:3734192/149‑1 (MQ=255)
                                                                                                                                 aaGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCAt               >  1:807413/1‑149 (MQ=255)
                                                                                                                                  aGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa              >  1:270799/1‑149 (MQ=255)
                                                                                                                                  aGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa              >  2:2880483/1‑149 (MQ=255)
                                                                                                                                   gATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaa             >  2:2289917/1‑149 (MQ=255)
                                                                                                                                      cGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAAc          <  1:546672/149‑1 (MQ=255)
                                                                                                                                         cAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGc       >  1:1586756/1‑149 (MQ=255)
                                                                                                                                           gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTg     >  2:37824/1‑149 (MQ=255)
                                                                                                                                           gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTg     >  1:3456527/1‑149 (MQ=255)
                                                                                                                                             gTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGt   >  2:1903210/1‑149 (MQ=255)
                                                                                                                                              tATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTc  <  2:729635/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTC  >  CP009273/2934698‑2934988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑