BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,950,594	G→T	5.7%	V550V (GTC→GTA)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,950,594	0	G	T	5.7%	1147.5 / 27.1	295	V550V (GTC→GTA)	ptrA	protease III
Reads supporting (aligned to +/- strand): ref base G (128/150); new base T (5/12); total (133/162)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 300 total aligned reads displayed.

TGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTG  >  CP009273/2950446‑2950742

 gttAGCGTTGGTGGAAAAACTTCTGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGa                                                                                                                                                     <  2:2396277/149‑1 (MQ=255)

   tAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACa                                                                                                                                                   <  1:1774123/149‑1 (MQ=255)

     gCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATc                                                                                                                                                 <  2:3235532/149‑1 (MQ=255)

      cGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCa                                                                                                                                                <  1:3650137/149‑1 (MQ=255)

       gTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAg                                                                                                                                               >  1:1855383/1‑149 (MQ=255)

         tggtggAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCTGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCt                                                                                                                                             <  2:4712167/149‑1 (MQ=255)

               aaaaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGc                                                                                                                                       <  2:3127044/149‑1 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     <  1:4043003/149‑1 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     <  1:4100212/149‑1 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     <  1:4104824/149‑1 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     <  1:774148/149‑1 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     >  2:262630/1‑149 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     <  2:2810700/149‑1 (MQ=255)

                 aaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                     >  2:262890/1‑149 (MQ=255)

                     ttATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTg                                                                                                                                 >  1:3545087/1‑149 (MQ=255)

                     ttATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTg                                                                                                                                 >  2:4296200/1‑149 (MQ=255)

                       aTGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGc                                                                                                                               >  1:4729444/1‑149 (MQ=255)

                        tGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTGTGGGCTCGCTGGCa                                                                                                                              >  2:4645665/1‑149 (MQ=255)

                         gCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCaa                                                                                                                             >  1:396545/1‑149 (MQ=255)

                           caccaACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCaaaa                                                                                                                           <  2:979822/149‑1 (MQ=255)

                             ccaACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATa                                                                                                                         >  2:2044900/1‑149 (MQ=255)

                               aaCCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAAc                                                                                                                       <  1:2942503/149‑1 (MQ=255)

                                  cGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCCAAATAACGGc                                                                                                                    >  2:3838374/1‑149 (MQ=255)

                                       ccTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGgc                                                                                                               <  2:84992/149‑1 (MQ=255)

                                         tGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGgcgc                                                                                                             >  2:3332808/1‑149 (MQ=255)

                                         tGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGgcgc                                                                                                             >  2:3623225/1‑149 (MQ=255)

                                           gTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCAt                                                                                                           <  1:3644513/149‑1 (MQ=255)

                                               cTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATacac                                                                                                       >  2:1844348/1‑149 (MQ=255)

                                                      gATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACgcgc                                                                                                >  1:1244403/1‑149 (MQ=255)

                                                      gATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACgcgc                                                                                                >  1:2402366/1‑149 (MQ=255)

                                                        tCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAg                                                                                              >  2:2481465/1‑149 (MQ=255)

                                                         cAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGa                                                                                             <  2:1589649/149‑1 (MQ=255)

                                                         cAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGa                                                                                             >  2:2211454/1‑149 (MQ=255)

                                                           aGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGAtt                                                                                           >  1:229045/1‑149 (MQ=255)

                                                           aGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGAtt                                                                                           <  2:2518151/149‑1 (MQ=255)

                                                               ccAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGAc                                                                                       >  1:2504013/1‑149 (MQ=255)

                                                               ccAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGAc                                                                                       <  2:1690789/149‑1 (MQ=255)

                                                               ccAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGAc                                                                                       >  1:3087718/1‑149 (MQ=255)

                                                                cAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACt                                                                                      >  1:1114563/1‑149 (MQ=255)

                                                                 aGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtc                                                                                     >  1:2742380/1‑149 (MQ=255)

                                                                  gCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtca                                                                                    >  1:85614/1‑149 (MQ=255)

                                                                  gCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtca                                                                                    >  2:1918327/1‑149 (MQ=255)

                                                                     cTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtcatca                                                                                 >  1:90102/1‑149 (MQ=255)

                                                                      tGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATcaa                                                                                <  1:2653578/149‑1 (MQ=255)

                                                                            aTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCa                                                                          >  2:3095204/1‑149 (MQ=255)

                                                                            aTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCa                                                                          >  2:1465012/1‑149 (MQ=255)

                                                                             tAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAg                                                                         <  2:1707647/149‑1 (MQ=255)

                                                                              aaTCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGc                                                                        <  2:3487947/149‑1 (MQ=255)

                                                                                tCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGctc                                                                      <  1:983532/149‑1 (MQ=255)

                                                                                tCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGctc                                                                      <  2:3708658/149‑1 (MQ=255)

                                                                                    tGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGa                                                                  <  1:1945172/149‑1 (MQ=255)

                                                                                     gAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCTAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGAt                                                                 >  2:1086508/1‑149 (MQ=255)

                                                                                       gcgcAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATgg                                                               >  1:2461069/1‑149 (MQ=255)

                                                                                           aaaCATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGt                                                           <  1:288761/149‑1 (MQ=255)

                                                                                             aCATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTAt                                                         <  2:1401365/149‑1 (MQ=255)

                                                                                              cATCACCTGAGTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTAtt                                                        <  2:1893501/149‑1 (MQ=255)

                                                                                               aTCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTAttt                                                       >  1:1622616/1‑149 (MQ=255)

                                                                                                 cACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttct                                                     <  1:3204867/149‑1 (MQ=255)

                                                                                                 cACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttct                                                     <  1:3268998/149‑1 (MQ=255)

                                                                                                   ccTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttcttc                                                   >  1:2122374/1‑149 (MQ=255)

                                                                                                     tGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTtctc                                                 <  1:3935998/149‑1 (MQ=255)

                                                                                                     tGATTGCGGGCGCTGTCCATGGCCTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTtctc                                                 <  2:1208912/149‑1 (MQ=255)

                                                                                                       aTTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTg                                               <  1:262890/149‑1 (MQ=255)

                                                                                                        gtGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGa                                              <  2:2182834/148‑1 (MQ=255)

                                                                                                         tGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGAc                                             <  1:3186300/149‑1 (MQ=255)

                                                                                                           cGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACtt                                           <  2:1364606/149‑1 (MQ=255)

                                                                                                              gcgcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAAt                                        <  1:2065110/149‑1 (MQ=255)

                                                                                                                gcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCa                                      >  2:1664766/1‑149 (MQ=255)

                                                                                                                 cTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                     >  1:591179/1‑149 (MQ=255)

                                                                                                                 cTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                     >  2:2871223/1‑149 (MQ=255)

                                                                                                                     ccATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgag                                 >  1:2428874/1‑149 (MQ=255)

                                                                                                                     ccATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTAAATCAGCgag                                 <  1:3420988/149‑1 (MQ=255)

                                                                                                                      cATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgaga                                >  1:3804234/1‑149 (MQ=255)

                                                                                                                      cATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgaga                                <  2:2717236/149‑1 (MQ=255)

                                                                                                                       aTGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGaa                               <  1:3275644/149‑1 (MQ=255)

                                                                                                                        tGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGaaa                              <  2:824645/149‑1 (MQ=255)

                                                                                                                        tGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGaaa                              <  2:360885/149‑1 (MQ=255)

                                                                                                                         ggCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAat                             <  2:4766495/149‑1 (MQ=255)

                                                                                                                           cTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatca                           <  1:1685600/149‑1 (MQ=255)

                                                                                                                                 gATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGa                     >  1:3900184/1‑149 (MQ=255)

                                                                                                                                 gATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGa                     >  2:730883/1‑149 (MQ=255)

                                                                                                                                 gATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGa                     >  2:4477608/1‑149 (MQ=255)

                                                                                                                                  aTTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGaa                    <  2:923594/149‑1 (MQ=255)

                                                                                                                                   ttACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAat                   >  1:4596173/1‑149 (MQ=255)

                                                                                                                                        cAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAg              <  2:1677904/149‑1 (MQ=255)

                                                                                                                                         aaaaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAgg             >  2:3940082/1‑149 (MQ=255)

                                                                                                                                          aaaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAggg            <  2:3863975/149‑1 (MQ=255)

                                                                                                                                          aaaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAggg            >  1:935722/1‑149 (MQ=255)

                                                                                                                                           aaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGt           <  1:1358250/149‑1 (MQ=255)

                                                                                                                                           aaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCACGAATATAAGGGt           >  2:4090459/1‑149 (MQ=255)

                                                                                                                                            aTCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGtt          >  1:4758396/1‑149 (MQ=255)

                                                                                                                                            aTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGtt          <  2:522618/149‑1 (MQ=255)

                                                                                                                                            aTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGtt          >  2:3372562/1‑149 (MQ=255)

                                                                                                                                            aTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAAATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGtt          >  1:3363495/1‑149 (MQ=255)

                                                                                                                                                ggCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGc      <  2:4360624/149‑1 (MQ=255)

                                                                                                                                                  cTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGctc    >  1:485100/1‑149 (MQ=255)

                                                                                                                                                   tGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGctct   >  1:4302166/1‑149 (MQ=255)

                                                                                                                                                   tGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGctct   <  2:2899257/149‑1 (MQ=255)

                                                                                                                                                    gACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTg  >  1:1418349/1‑149 (MQ=255)

                                                                                                                                                    gACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTg  >  2:3055767/1‑149 (MQ=255)

TGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTG  >  CP009273/2950446‑2950742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`