Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,959,004 G→A 100% I131I (ATC→ATT)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,959,0040GA98.6% 957.6 / 2.1 280I131I (ATC→ATT) lgtphosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base A (134/142);  total (134/146)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

TTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCG  >  CP009273/2958858‑2959149
                                                                                                                                                  |                                                                                                                                                 
ttggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCACCGCGGCCCCACCATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCACGACCAAACGGAATGAGTGGTGCAAt                                                                                                                                                 >  2:4662540/1‑149 (MQ=255)
  ggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaa                                                                                                                                               <  1:3599792/149‑1 (MQ=255)
  ggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaa                                                                                                                                               <  1:3115283/149‑1 (MQ=255)
   gttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaa                                                                                                                                              >  2:2480053/1‑149 (MQ=255)
   gttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaa                                                                                                                                              >  1:2780084/1‑149 (MQ=255)
    tttGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaaa                                                                                                                                             <  1:2549690/149‑1 (MQ=255)
         agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGACTGAGTGGTGCAATAAAATCaga                                                                                                                                        >  1:88783/1‑149 (MQ=255)
         agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCaga                                                                                                                                        >  2:2771931/1‑149 (MQ=255)
         agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCaga                                                                                                                                        <  2:4706523/149‑1 (MQ=255)
         agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCaga                                                                                                                                        <  2:4743191/149‑1 (MQ=255)
          gcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCagag                                                                                                                                       <  1:2010163/149‑1 (MQ=255)
             gcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGAcc                                                                                                                                    <  2:1253795/149‑1 (MQ=255)
             gcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGAcc                                                                                                                                    >  1:4021978/1‑149 (MQ=255)
             gcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGAcc                                                                                                                                    >  1:692668/1‑149 (MQ=255)
             gcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGAcc                                                                                                                                    <  2:26591/149‑1 (MQ=255)
               aaaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTg                                                                                                                                  <  1:2882591/149‑1 (MQ=255)
               aaaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTg                                                                                                                                  >  1:116299/1‑149 (MQ=255)
               aaaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTg                                                                                                                                  <  1:2107502/149‑1 (MQ=255)
                aaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTgg                                                                                                                                 >  1:3975307/1‑149 (MQ=255)
                aaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTgg                                                                                                                                 >  1:4179258/1‑149 (MQ=255)
                aaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTgg                                                                                                                                 >  1:1585242/1‑149 (MQ=255)
                aaaTATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTgg                                                                                                                                 >  1:1531578/1‑149 (MQ=255)
                 aaTATCTTCTGTACGGGAGCCAGGGAGCAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGga                                                                                                                                >  1:2740852/1‑149 (MQ=255)
                  atatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaa                                                                                                                               >  1:4729423/1‑149 (MQ=255)
                  atatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCAGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaa                                                                                                                               <  1:3823658/149‑1 (MQ=255)
                   tatCTTCTGTACGGGAGCCAGGGAACAGCATGGAAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATATAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaag                                                                                                                              <  1:3438885/149‑1 (MQ=255)
                    atCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaaga                                                                                                                             >  1:4745162/1‑149 (MQ=255)
                     tcttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTTATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaagaa                                                                                                                            <  2:1055181/149‑1 (MQ=255)
                     tcttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaagaa                                                                                                                            >  1:4011403/1‑149 (MQ=255)
                       ttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAgg                                                                                                                          >  2:173139/1‑149 (MQ=255)
                       ttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAgg                                                                                                                          <  1:2235954/149‑1 (MQ=255)
                         ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGaa                                                                                                                        <  1:320956/149‑1 (MQ=255)
                         ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGaa                                                                                                                        <  2:373550/149‑1 (MQ=255)
                            tACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGt                                                                                                                     >  1:2439575/1‑149 (MQ=255)
                             aCGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGtt                                                                                                                    <  1:4432963/149‑1 (MQ=255)
                              cGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGttt                                                                                                                   <  1:3460750/149‑1 (MQ=255)
                               gggAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTa                                                                                                                  >  2:424392/1‑149 (MQ=255)
                                  aGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTa                                                                                                               <  2:2053638/149‑1 (MQ=255)
                                     cAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcgg                                                                                                            <  2:3219735/149‑1 (MQ=255)
                                       gggAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATACAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcg                                                                                                          >  2:1664097/1‑149 (MQ=255)
                                          aaCAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACggcgggc                                                                                                       <  1:597883/149‑1 (MQ=255)
                                          aaCAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACggcgggc                                                                                                       <  1:2772496/149‑1 (MQ=255)
                                          aaCAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGGTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACggcgggc                                                                                                       >  1:4288747/1‑149 (MQ=255)
                                             aGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGaa                                                                                                    <  1:426675/149‑1 (MQ=255)
                                              gCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAg                                                                                                   <  1:4543464/149‑1 (MQ=255)
                                              gCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAg                                                                                                   >  2:4142287/1‑149 (MQ=255)
                                                aTGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGAt                                                                                                 >  1:2670975/1‑149 (MQ=255)
                                                  ggCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATaa                                                                                               >  2:4283550/1‑149 (MQ=255)
                                                    cAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAatc                                                                                             <  2:3849307/149‑1 (MQ=255)
                                                           gAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATcacca                                                                                      >  1:4664568/1‑149 (MQ=255)
                                                              gTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGa                                                                                   <  1:295521/149‑1 (MQ=255)
                                                                 cGGGTCAACGCGGCCCCACAATTCACCGTTAATACAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATaa                                                                                <  2:2434729/149‑1 (MQ=255)
                                                                 cGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATaa                                                                                <  2:3078692/149‑1 (MQ=255)
                                                                 cGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATaa                                                                                <  2:3078558/149‑1 (MQ=255)
                                                                  gggTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAAc                                                                               >  2:313035/1‑149 (MQ=255)
                                                                     tCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGcc                                                                            <  1:2803407/149‑1 (MQ=255)
                                                                     tCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGcc                                                                            <  1:1296737/149‑1 (MQ=255)
                                                                          gcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCa                                                                       >  1:1666170/1‑149 (MQ=255)
                                                                          gcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCa                                                                       <  2:4418485/149‑1 (MQ=255)
                                                                            ggCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAgg                                                                     <  1:3877447/149‑1 (MQ=255)
                                                                               cccACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccg                                                                  >  1:861216/1‑149 (MQ=255)
                                                                                ccACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgc                                                                 <  1:4707146/149‑1 (MQ=255)
                                                                                ccACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgc                                                                 >  1:3034169/1‑149 (MQ=255)
                                                                                 cacaATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgcc                                                                <  2:1285754/149‑1 (MQ=255)
                                                                                 cacaATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgcc                                                                >  1:3340886/1‑149 (MQ=255)
                                                                                   caATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGt                                                              >  2:540080/1‑149 (MQ=255)
                                                                                   caATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGt                                                              >  2:578305/1‑149 (MQ=255)
                                                                                           cGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGAc                                                      >  2:1477234/1‑149 (MQ=255)
                                                                                            gTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACa                                                     <  1:1062003/149‑1 (MQ=255)
                                                                                            gTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACa                                                     <  2:3596439/149‑1 (MQ=255)
                                                                                             ttAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACAt                                                    <  2:1806000/149‑1 (MQ=255)
                                                                                               aataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgc                                                  >  2:1818427/1‑149 (MQ=255)
                                                                                               aataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgc                                                  <  1:4377158/149‑1 (MQ=255)
                                                                                                ataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgcc                                                 >  1:2156134/1‑149 (MQ=255)
                                                                                                     gTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGt                                            >  1:4480616/1‑149 (MQ=255)
                                                                                                       tGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTcc                                          <  1:490565/149‑1 (MQ=255)
                                                                                                          ccAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAg                                       >  1:1273043/1‑149 (MQ=255)
                                                                                                              aCGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGacac                                   >  2:393527/1‑149 (MQ=255)
                                                                                                                  ccGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGaa                               <  2:850944/149‑1 (MQ=255)
                                                                                                                  ccGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGaa                               <  2:3238985/149‑1 (MQ=255)
                                                                                                                   cGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAAc                              <  2:3840599/149‑1 (MQ=255)
                                                                                                                    ggCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACa                             >  2:2744569/1‑149 (MQ=255)
                                                                                                                      cACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGa                           <  2:2603083/149‑1 (MQ=255)
                                                                                                                      cACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGa                           <  2:279944/149‑1 (MQ=255)
                                                                                                                         cAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATAc                        <  1:378344/149‑1 (MQ=255)
                                                                                                                          aaGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACa                       >  1:1803008/1‑149 (MQ=255)
                                                                                                                            gACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGc                     <  1:2295002/149‑1 (MQ=255)
                                                                                                                              ccAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCgg                   <  1:2367506/149‑1 (MQ=255)
                                                                                                                               cAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGa                  >  2:1958658/1‑149 (MQ=255)
                                                                                                                                aaaCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGAt                 <  2:4021978/149‑1 (MQ=255)
                                                                                                                                 aaCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGacc                <  2:2309408/149‑3 (MQ=255)
                                                                                                                                 aaCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATc                <  2:2309572/149‑1 (MQ=255)
                                                                                                                                  aCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCg               >  1:1204545/1‑149 (MQ=255)
                                                                                                                                     gAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGcc            >  2:3942344/1‑149 (MQ=255)
                                                                                                                                      aaTGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCa           >  2:1267622/1‑149 (MQ=255)
                                                                                                                                         gAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATaa        >  1:1738028/1‑149 (MQ=255)
                                                                                                                                         gAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATaa        >  2:3492948/1‑149 (MQ=255)
                                                                                                                                          aGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa       <  2:4590779/149‑1 (MQ=255)
                                                                                                                                          aGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa       <  2:72531/149‑1 (MQ=255)
                                                                                                                                             ggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTg    <  2:1449504/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCG  >  CP009273/2958858‑2959149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑