Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 25,546 G→T 100% D114Y (GAC→TAC)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927325,5460GT98.5% 868.6 / 3.4 265D114Y (GAC→TAC) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (126/135);  total (127/138)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 265 total aligned reads displayed.

GCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGA  >  CP009273/25400‑25690
                                                                                                                                                  |                                                                                                                                                
gcggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTAc                                                                                                                                                <  1:412820/149‑1 (MQ=255)
     tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCt                                                                                                                                           >  1:2638865/1‑149 (MQ=255)
      ggCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtg                                                                                                                                          >  1:2777181/1‑149 (MQ=255)
       gCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtgt                                                                                                                                         >  2:791909/1‑149 (MQ=255)
        cAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtgtg                                                                                                                                        <  2:298286/149‑1 (MQ=255)
           cGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCa                                                                                                                                     <  2:2190515/149‑1 (MQ=255)
            gTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCAc                                                                                                                                    <  2:1294659/149‑1 (MQ=255)
             ttGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACg                                                                                                                                   >  2:3878296/1‑149 (MQ=255)
              tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACgg                                                                                                                                  >  1:4117996/1‑149 (MQ=255)
                    tttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGt                                                                                                                            <  2:2521801/149‑1 (MQ=255)
                    tttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGt                                                                                                                            >  1:3921688/1‑149 (MQ=255)
                    tttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGt                                                                                                                            <  1:242032/149‑1 (MQ=255)
                      tGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTg                                                                                                                          <  1:1797412/149‑1 (MQ=255)
                       gCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGt                                                                                                                         <  1:4646549/149‑1 (MQ=255)
                           gTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGat                                                                                                                     >  2:4654937/1‑149 (MQ=255)
                             aTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGatat                                                                                                                   <  2:4165467/149‑1 (MQ=255)
                                gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGAt                                                                                                                <  1:3136121/149‑1 (MQ=255)
                                gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGAt                                                                                                                <  1:3722884/149‑1 (MQ=255)
                                   aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                             >  2:173078/1‑149 (MQ=255)
                                    ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGAc                                                                                                            <  1:4719391/149‑1 (MQ=255)
                                     tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACt                                                                                                           <  1:335632/149‑1 (MQ=255)
                                      ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACtt                                                                                                          >  2:3407403/1‑149 (MQ=255)
                                      ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACtt                                                                                                          >  2:1018763/1‑149 (MQ=255)
                                       gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                         <  1:1438086/149‑1 (MQ=255)
                                        tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCt                                                                                                        >  1:744122/1‑149 (MQ=255)
                                          ttAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTAc                                                                                                      >  2:1145582/1‑149 (MQ=255)
                                            aGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGt                                                                                                    <  1:2397186/149‑1 (MQ=255)
                                             gCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTc                                                                                                   <  2:4252668/149‑1 (MQ=255)
                                               gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCgg                                                                                                 <  2:4410695/149‑1 (MQ=255)
                                                 gATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCg                                                                                               >  2:974840/1‑149 (MQ=255)
                                                  aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGa                                                                                              >  2:1660333/1‑149 (MQ=255)
                                                  aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGa                                                                                              >  2:4048647/1‑149 (MQ=255)
                                                   tCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGAc                                                                                             >  1:2228573/1‑149 (MQ=255)
                                                    ccTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                            <  1:4721749/149‑1 (MQ=255)
                                                     cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTg                                                                                           <  2:4264099/149‑1 (MQ=255)
                                                       ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGc                                                                                         >  2:4775316/1‑149 (MQ=255)
                                                        gCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCa                                                                                        <  1:2116792/149‑1 (MQ=255)
                                                         cAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCAc                                                                                       >  2:50644/1‑149 (MQ=255)
                                                           gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACtt                                                                                     <  1:70289/149‑1 (MQ=255)
                                                             gatgatGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                   >  1:4487178/1‑149 (MQ=255)
                                                                  tgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCAcc                                                                              >  2:1824701/1‑149 (MQ=255)
                                                                    tATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCtt                                                                            >  2:1464030/1‑149 (MQ=255)
                                                                     aTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTc                                                                           <  2:3699672/149‑1 (MQ=255)
                                                                     aTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTc                                                                           <  1:963638/149‑1 (MQ=255)
                                                                      tCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCa                                                                          >  1:3478416/1‑149 (MQ=255)
                                                                       cGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCaa                                                                         <  1:3115493/149‑1 (MQ=255)
                                                                          tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCt                                                                      <  2:2230037/149‑1 (MQ=255)
                                                                           cGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCtt                                                                     >  1:2962418/1‑149 (MQ=255)
                                                                            gAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTg                                                                    <  2:8695/149‑1 (MQ=255)
                                                                            gAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTg                                                                    >  2:4496933/1‑149 (MQ=255)
                                                                             aaGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGc                                                                   <  1:3854289/149‑1 (MQ=255)
                                                                               ggCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCg                                                                 >  1:4273762/1‑149 (MQ=255)
                                                                                   aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATAc                                                             <  2:3311229/149‑1 (MQ=255)
                                                                                   aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATAc                                                             >  2:1839496/1‑149 (MQ=255)
                                                                                    cGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACt                                                            <  1:1401354/149‑1 (MQ=255)
                                                                                     gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTg                                                           >  1:205477/1‑149 (MQ=255)
                                                                                      cAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGc                                                          >  2:4307897/1‑149 (MQ=255)
                                                                                       aGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                         >  2:3513864/1‑149 (MQ=255)
                                                                                        gAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                        >  2:678657/1‑149 (MQ=255)
                                                                                         aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCAt                                                       >  2:4035733/1‑149 (MQ=255)
                                                                                          aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATc                                                      <  2:630036/149‑1 (MQ=255)
                                                                                          aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATc                                                      >  2:586526/1‑149 (MQ=255)
                                                                                            cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                    <  2:2884698/149‑1 (MQ=255)
                                                                                              aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                  <  1:149484/149‑1 (MQ=255)
                                                                                               aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                 >  1:85208/1‑149 (MQ=255)
                                                                                                 cAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCg                                               >  1:2591640/1‑149 (MQ=255)
                                                                                                  aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCgg                                              <  2:1059659/149‑1 (MQ=255)
                                                                                                  aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCgg                                              <  1:1149260/149‑1 (MQ=255)
                                                                                                   atatCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGt                                             >  1:4053327/1‑149 (MQ=255)
                                                                                                   atatCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGt                                             <  1:4291651/149‑1 (MQ=255)
                                                                                                     atCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGc                                           >  2:2496286/1‑149 (MQ=255)
                                                                                                      tCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCg                                          >  2:1597847/1‑149 (MQ=255)
                                                                                                         cTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCa                                       >  1:4690415/1‑149 (MQ=255)
                                                                                                         cTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCa                                       >  2:167595/1‑149 (MQ=255)
                                                                                                          ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCAc                                      <  2:4189570/149‑1 (MQ=255)
                                                                                                          ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCAc                                      <  2:2544359/149‑1 (MQ=255)
                                                                                                          ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCAc                                      >  2:1139580/1‑149 (MQ=255)
                                                                                                                gcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtg                                <  1:3067029/149‑1 (MQ=255)
                                                                                                                 cTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                               >  1:3956841/1‑149 (MQ=255)
                                                                                                                  tGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                              >  1:1929332/1‑149 (MQ=255)
                                                                                                                   gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGc                             <  1:2540362/149‑1 (MQ=255)
                                                                                                                    attattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCt                            >  2:1500864/1‑149 (MQ=255)
                                                                                                                          ggcggcGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                      <  2:26721/149‑1 (MQ=255)
                                                                                                                          ggcggcGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                      <  2:26376/149‑1 (MQ=255)
                                                                                                                          ggcggcGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                      >  1:1625962/1‑149 (MQ=255)
                                                                                                                          ggcggcGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                      <  1:549845/149‑1 (MQ=255)
                                                                                                                             ggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttt                   >  1:3689230/1‑149 (MQ=255)
                                                                                                                             ggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttt                   <  1:577639/149‑1 (MQ=255)
                                                                                                                             ggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttt                   <  2:903069/149‑1 (MQ=255)
                                                                                                                              gcgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                  <  1:287986/149‑1 (MQ=255)
                                                                                                                                   cTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGcc             <  2:244075/149‑1 (MQ=255)
                                                                                                                                    tGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCt            <  1:442921/149‑1 (MQ=255)
                                                                                                                                    tGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCt            <  1:4352408/149‑1 (MQ=255)
                                                                                                                                     gggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCtt           >  2:4541901/1‑149 (MQ=255)
                                                                                                                                      ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTc          <  1:50644/149‑1 (MQ=255)
                                                                                                                                      ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTc          <  2:3901511/149‑1 (MQ=255)
                                                                                                                                      ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTc          <  1:1018763/149‑1 (MQ=255)
                                                                                                                                           ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTagag     >  2:3357377/1‑149 (MQ=255)
                                                                                                                                           ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTagag     >  1:3485524/1‑149 (MQ=255)
                                                                                                                                              gTTCTACCGCCTGTGGCACGGCTTCTATGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTACGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAGTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGa  >  2:3152271/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGA  >  CP009273/25400‑25690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑