BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,983,183	C→A	16.1%	D26Y (GAT→TAT)	yqeL ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,983,183	0	C	A	16.1%	771.2 / 91.3	255	D26Y (GAT→TAT)	yqeL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (118/96); new base A (29/12); total (147/108)
Fisher's exact test for biased strand distribution p-value = 8.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 257 total aligned reads displayed.

GATTTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCG  >  CP009273/2983035‑2983330

     ttATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATaaa                                                                                                                                                <  1:4502733/149‑1 (MQ=255)

      tATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAACTAGATAACAAAAAGATGGAACCTCGTTAATAATaaaa                                                                                                                                               <  2:1927049/149‑1 (MQ=255)

       aTGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAAc                                                                                                                                              <  2:2103051/149‑1 (MQ=255)

         gTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACaa                                                                                                                                            <  1:3447169/149‑1 (MQ=255)

             ttCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATaaa                                                                                                                                        <  2:900048/149‑1 (MQ=255)

              tCCGGAAAAAAGTGTAAGCTATAATAAACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAACGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATaaaa                                                                                                                                       >  1:2063706/1‑149 (MQ=255)

              tCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATaaaa                                                                                                                                       >  1:4402226/1‑149 (MQ=255)

               ccAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAAt                                                                                                                                      <  2:4270696/149‑1 (MQ=255)

               ccAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAAt                                                                                                                                      <  2:1673383/149‑1 (MQ=255)

               ccAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAAt                                                                                                                                      <  1:1993782/149‑1 (MQ=255)

                  gAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTaa                                                                                                                                   >  2:3394541/1‑149 (MQ=255)

                   aaaaaaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTaaa                                                                                                                                  >  1:1719161/1‑149 (MQ=255)

                    aaaaaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAat                                                                                                                                 >  1:56231/1‑149 (MQ=255)

                    aaaaaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAat                                                                                                                                 >  2:2260175/1‑149 (MQ=255)

                     aaaaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAata                                                                                                                                >  2:2732861/1‑149 (MQ=255)

                     aaaaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAata                                                                                                                                <  2:1021847/149‑1 (MQ=255)

                     aaaaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAata                                                                                                                                >  2:2732982/1‑149 (MQ=255)

                       aaGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATAtt                                                                                                                              <  2:40299/149‑1 (MQ=255)

                          tgtAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATtctc                                                                                                                           <  2:79584/149‑1 (MQ=255)

                          tgtAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATtctc                                                                                                                           <  2:4776288/149‑1 (MQ=255)

                              aGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAg                                                                                                                       >  1:1068219/1‑149 (MQ=255)

                              aGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAg                                                                                                                       >  1:3595324/1‑149 (MQ=255)

                              aGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAg                                                                                                                       >  2:1524309/1‑149 (MQ=255)

                               gCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGt                                                                                                                      >  1:2413168/1‑149 (MQ=255)

                               gCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGt                                                                                                                      >  1:654663/1‑149 (MQ=255)

                                   taataTACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTatat                                                                                                                  <  2:927457/149‑1 (MQ=255)

                                     atatACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATgg                                                                                                                >  2:3685697/1‑149 (MQ=255)

                                     atatACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATgg                                                                                                                >  1:3780339/1‑149 (MQ=255)

                                        taCTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAg                                                                                                             <  1:1357324/149‑1 (MQ=255)

                                        taCTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAg                                                                                                             <  1:3746176/149‑1 (MQ=255)

                                        taCTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAg                                                                                                             <  1:4499405/149‑1 (MQ=255)

                                         aCTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGt                                                                                                            >  2:2806457/1‑149 (MQ=255)

                                         aCTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGt                                                                                                            >  2:133287/1‑149 (MQ=255)

                                           tAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCt                                                                                                          >  1:3174905/1‑149 (MQ=255)

                                              aGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTaaa                                                                                                       <  1:1706201/149‑1 (MQ=255)

                                              aGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTaaa                                                                                                       >  2:4687423/1‑149 (MQ=255)

                                                cAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGc                                                                                                     <  1:4067747/149‑1 (MQ=255)

                                                   ggTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATc                                                                                                  >  2:3483747/1‑149 (MQ=255)

                                                   ggTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATc                                                                                                  <  2:4594285/149‑1 (MQ=255)

                                                       aGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAg                                                                                              >  2:3522415/1‑149 (MQ=255)

                                                       aGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAg                                                                                              >  2:4415258/1‑149 (MQ=255)

                                                        gCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGa                                                                                             <  2:2361724/149‑1 (MQ=255)

                                                        gCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGa                                                                                             >  1:3810537/1‑149 (MQ=255)

                                                          aTGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGAtt                                                                                           >  2:148891/1‑149 (MQ=255)

                                                           tGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGAttt                                                                                          <  2:1763625/149‑1 (MQ=255)

                                                             cTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGa                                                                                        >  2:1104494/1‑149 (MQ=255)

                                                             cTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGCTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGa                                                                                        >  2:601090/1‑149 (MQ=255)

                                                               tttATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATc                                                                                      <  2:1221030/149‑1 (MQ=255)

                                                               tttATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATc                                                                                      >  2:2994954/1‑149 (MQ=255)

                                                                 tATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCaa                                                                                    <  1:1141536/149‑1 (MQ=255)

                                                                  aTTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAAc                                                                                   >  1:370016/1‑149 (MQ=255)

                                                                  aTTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAAATTTGATCAAc                                                                                   >  1:1867921/1‑149 (MQ=255)

                                                                       gAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCtt                                                                              <  2:4388519/149‑1 (MQ=255)

                                                                       gAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCtt                                                                              >  2:2153310/1‑149 (MQ=255)

                                                                        aGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCttt                                                                             >  2:331172/1‑149 (MQ=255)

                                                                         gCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTc                                                                            >  2:2825411/1‑149 (MQ=255)

                                                                          cATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCa                                                                           <  2:4242557/149‑1 (MQ=255)

                                                                          cATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCa                                                                           <  2:3595711/149‑1 (MQ=255)

                                                                           aTTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCAt                                                                          <  1:2292463/149‑1 (MQ=255)

                                                                            ttGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCAtt                                                                         >  2:4351938/1‑149 (MQ=255)

                                                                              ggTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCAtttt                                                                       >  1:396858/1‑149 (MQ=255)

                                                                                tAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAg                                                                     >  1:2940440/1‑149 (MQ=255)

                                                                                 aaCAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAGAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGa                                                                    >  1:1190809/1‑149 (MQ=255)

                                                                                   cAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACa                                                                  >  1:1066944/1‑149 (MQ=255)

                                                                                       gCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACAtctc                                                              <  1:3390621/149‑1 (MQ=255)

                                                                                          ataatGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCtt                                                           >  2:3195402/1‑149 (MQ=255)

                                                                                           taatGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTg                                                          >  2:3875460/1‑149 (MQ=255)

                                                                                            aatGCATTGTGCAAGGTAAACTAAAAGAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGc                                                         <  1:4206770/149‑1 (MQ=255)

                                                                                               gCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAAt                                                      >  2:1140392/1‑149 (MQ=255)

                                                                                                  ttGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTa                                                   <  2:4102708/149‑1 (MQ=255)

                                                                                                   tgtgCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTaa                                                  <  1:1429430/149‑1 (MQ=255)

                                                                                                     tgCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTaaaa                                                >  1:2615652/1‑149 (MQ=255)

                                                                                                     tgCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTaaaa                                                <  1:3576383/149‑1 (MQ=255)

                                                                                                     tgCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTaaaa                                                >  1:435845/1‑149 (MQ=255)

                                                                                                      gCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAat                                               >  2:3048152/1‑149 (MQ=255)

                                                                                                        aaGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAatat                                             >  1:298762/1‑149 (MQ=255)

                                                                                                        aaGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAatat                                             >  2:2597367/1‑149 (MQ=255)

                                                                                                        aaGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAACTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAatat                                             >  1:1567026/1‑149 (MQ=255)

                                                                                                              aaCTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTa                                       <  2:3595324/149‑1 (MQ=255)

                                                                                                                cTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATc                                     <  2:1898979/149‑1 (MQ=255)

                                                                                                                 tAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATca                                    <  2:975101/149‑1 (MQ=255)

                                                                                                                  aaaaTAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATcaa                                   >  1:108654/1‑149 (MQ=255)

                                                                                                                     aTAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCACTGTAAAATATCATGTATcaacaa                                >  2:3815925/1‑149 (MQ=255)

                                                                                                                     aTAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATcaacaa                                >  1:4716148/1‑149 (MQ=255)

                                                                                                                     aTAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATcaacaa                                >  1:1804537/1‑149 (MQ=255)

                                                                                                                      tAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCaacaac                               >  1:52117/1‑149 (MQ=255)

                                                                                                                      tAGATAACAAAAAGATGGAACCTCGTTAATAATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCaacaac                               <  2:4182251/149‑1 (MQ=255)

                                                                                                                         aTAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATc                            <  2:1251152/149‑1 (MQ=255)

                                                                                                                          tAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCt                           >  1:858855/1‑149 (MQ=255)

                                                                                                                           aaCAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTg                          <  2:354781/149‑1 (MQ=255)

                                                                                                                               aaaaGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGc                      <  2:449383/149‑1 (MQ=255)

                                                                                                                                aaaGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCa                     >  2:527980/1‑149 (MQ=255)

                                                                                                                                      ggAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCa               >  1:1676513/1‑149 (MQ=255)

                                                                                                                                       gAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCAt              >  2:4533998/1‑149 (MQ=255)

                                                                                                                                            tCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTc         <  1:1287481/149‑1 (MQ=255)

                                                                                                                                              gTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGa       >  1:941637/1‑149 (MQ=255)

                                                                                                                                               ttAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGaa      >  2:2022551/1‑149 (MQ=255)

                                                                                                                                               ttAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGaa      >  2:3454716/1‑149 (MQ=255)

                                                                                                                                                  aTCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTc   >  1:2779766/1‑149 (MQ=255)

                                                                                                                                                   tCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCg  >  1:3617921/1‑149 (MQ=255)

GATTTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATGCTTTTATTTTGAGCATTGGTAACAGAGCAATAATGCATTGTGCAAGGTAAACTAAAATAGATAACAAAAAGATGGAACCTCGTTAATCATAAAACAATAAAATTAAATATTCTCGCAGTATATGGCAGTCTAAAGCATCAAAGATTTGATCAACATCTTTCATTTTAGACATCTCCTTGCAATGTAAAATATCATGTATCAACAACATCTGGTGCAAATCCATGAGTCGAACTCG  >  CP009273/2983035‑2983330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`