Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,017,285 G→T 100% D871Y (GAC→TAC)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,017,2850GT98.5% 907.7 / 3.0 270D871Y (GAC→TAC) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (147/119);  total (151/119)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

ACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATC  >  CP009273/3017139‑3017428
                                                                                                                                                  |                                                                                                                                               
acacGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTAc                                                                                                                                               <  2:3188994/149‑1 (MQ=255)
 cacGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACg                                                                                                                                              >  1:2741361/1‑149 (MQ=255)
  acGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGc                                                                                                                                             <  1:2831648/149‑1 (MQ=255)
  acGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGc                                                                                                                                             <  2:2302917/149‑1 (MQ=255)
   cgcgTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGcc                                                                                                                                            <  2:1781420/149‑1 (MQ=255)
      gTACGGGTGAAATCCGCCGGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAACTCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGaa                                                                                                                                         <  2:2612135/149‑1 (MQ=255)
        aCGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAgg                                                                                                                                       <  2:633392/149‑1 (MQ=255)
          gggTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTc                                                                                                                                     <  1:1008967/149‑1 (MQ=255)
          gggTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTc                                                                                                                                     <  2:568289/149‑1 (MQ=255)
            gTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCAc                                                                                                                                   >  1:2697237/1‑149 (MQ=255)
              gAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCAccc                                                                                                                                 >  2:1383744/1‑149 (MQ=255)
                aaTCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGt                                                                                                                               >  2:2101695/1‑149 (MQ=255)
                   ccGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTaa                                                                                                                            <  1:793115/149‑1 (MQ=255)
                   ccGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTaa                                                                                                                            <  2:2553407/149‑1 (MQ=255)
                   ccGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTaa                                                                                                                            <  2:774873/149‑1 (MQ=255)
                     gCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcg                                                                                                                          >  1:4544181/1‑149 (MQ=255)
                      ccTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcgc                                                                                                                         >  1:1745731/1‑149 (MQ=255)
                      ccTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAAcgc                                                                                                                         >  1:538555/1‑149 (MQ=255)
                         ggATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                      <  2:3766084/149‑1 (MQ=255)
                         ggATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTg                                                                                                                      >  1:3937926/1‑149 (MQ=255)
                          gATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGa                                                                                                                     <  2:4627712/149‑1 (MQ=255)
                          gATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGa                                                                                                                     <  1:1167785/149‑1 (MQ=255)
                             aaaTTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGAttt                                                                                                                  <  2:2573071/149‑1 (MQ=255)
                               aTTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTAc                                                                                                                <  1:3365437/149‑1 (MQ=255)
                                    aCGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGt                                                                                                           <  1:641644/149‑1 (MQ=255)
                                    aCGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGt                                                                                                           <  1:1063547/149‑1 (MQ=255)
                                    aCGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGt                                                                                                           >  2:3128775/1‑149 (MQ=255)
                                        cTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACg                                                                                                       >  1:3030980/1‑149 (MQ=255)
                                        cTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACg                                                                                                       >  2:4433571/1‑149 (MQ=255)
                                         ttGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACgg                                                                                                      <  2:919395/149‑1 (MQ=255)
                                               gACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCAcc                                                                                                >  1:3305577/1‑149 (MQ=255)
                                                 cTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGGTCCGGCGCACCGa                                                                                              >  2:3112314/1‑149 (MQ=255)
                                                     ggTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAAt                                                                                          <  2:644295/149‑1 (MQ=255)
                                                       tACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTg                                                                                        >  1:2350523/1‑149 (MQ=255)
                                                             ggTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACa                                                                                  <  2:957766/149‑1 (MQ=255)
                                                              gTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAt                                                                                 <  1:3109396/149‑1 (MQ=255)
                                                              gTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAt                                                                                 <  1:1571330/149‑1 (MQ=255)
                                                               tCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAtt                                                                                <  1:1383744/149‑1 (MQ=255)
                                                               tCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACAtt                                                                                <  1:3849554/149‑1 (MQ=255)
                                                                 aaTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTcc                                                                              >  2:3681722/1‑149 (MQ=255)
                                                                 aaTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTcc                                                                              >  1:2122620/1‑149 (MQ=255)
                                                                 aaTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTcc                                                                              >  1:2122686/1‑149 (MQ=255)
                                                                  aTCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcg                                                                             >  1:240425/1‑149 (MQ=255)
                                                                    ccAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgcg                                                                           >  1:3224677/1‑149 (MQ=255)
                                                                     cAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGt                                                                          >  2:2730445/1‑149 (MQ=255)
                                                                       gagTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGa                                                                        >  2:21232/1‑149 (MQ=255)
                                                                       gagTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGa                                                                        <  1:1833064/149‑1 (MQ=255)
                                                                        agTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAt                                                                       <  2:4069045/149‑1 (MQ=255)
                                                                         gTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGAtt                                                                      >  2:2926655/1‑149 (MQ=255)
                                                                            aGCGTTGGGACAACTCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTcc                                                                   <  2:3898658/149‑1 (MQ=255)
                                                                              cGTTGGGACAAATCTACGGTGTCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCcgc                                                                 >  1:2277664/1‑149 (MQ=255)
                                                                               gTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGACAATTGGTGACATTCCGCGTGATTTCcgcg                                                                >  1:4026007/1‑149 (MQ=255)
                                                                               gTTGGGACAAAACTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTAACGCAGTTACGGCGCACCGAACATTGGTGACATTCCGCGTGATTTCcgcg                                                                >  2:422799/1‑149 (MQ=255)
                                                                                  gggACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCtg                                                             >  2:2347496/1‑149 (MQ=255)
                                                                                   ggACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCtgt                                                            <  2:2516908/149‑1 (MQ=255)
                                                                                       aaaTCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTg                                                        <  1:1338296/149‑1 (MQ=255)
                                                                                       aaaTCTACGGTGCCACCCTGCGTGCGATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTg                                                        <  1:3783308/149‑1 (MQ=255)
                                                                                        aaTCTACGGAGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTgg                                                       >  2:4023844/1‑149 (MQ=255)
                                                                                          tCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTg                                                     <  1:847310/149‑1 (MQ=255)
                                                                                               ggTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAg                                                <  1:951456/149‑1 (MQ=255)
                                                                                                gTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGc                                               >  2:1021223/1‑149 (MQ=255)
                                                                                                gTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGc                                               >  1:1498584/1‑149 (MQ=255)
                                                                                                        cTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaa                                       >  2:4512040/1‑149 (MQ=255)
                                                                                                            gTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTc                                   <  2:1291648/149‑1 (MQ=255)
                                                                                                            gTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTc                                   >  2:1510713/1‑149 (MQ=255)
                                                                                                             tGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCg                                  >  2:170333/1‑149 (MQ=255)
                                                                                                              gCTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCgg                                 >  1:3703789/1‑149 (MQ=255)
                                                                                                               cTATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGc                                <  1:4317041/149‑1 (MQ=255)
                                                                                                                tATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                               <  1:722987/149‑1 (MQ=255)
                                                                                                                tATCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                               <  1:1689357/149‑1 (MQ=255)
                                                                                                                 aTCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATCAAGTCGGccc                              >  1:3892308/1‑149 (MQ=255)
                                                                                                                 aTCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGccc                              >  1:3619946/1‑149 (MQ=255)
                                                                                                                 aTCGGCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGccc                              <  2:3585489/149‑1 (MQ=255)
                                                                                                                   cGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGt                            >  2:3348739/1‑149 (MQ=255)
                                                                                                                     gCCACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTc                          >  1:3527340/1‑149 (MQ=255)
                                                                                                                      ccACAGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCg                         <  1:424636/149‑1 (MQ=255)
                                                                                                                        acaGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                       >  2:4392101/1‑149 (MQ=255)
                                                                                                                        acaGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                       >  2:1351071/1‑149 (MQ=255)
                                                                                                                        acaGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                       >  2:1500376/1‑149 (MQ=255)
                                                                                                                         caGTATGAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGTCATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgggg                      <  2:2817234/149‑1 (MQ=255)
                                                                                                                               gAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                >  1:4336484/1‑149 (MQ=255)
                                                                                                                               gAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                <  2:1073492/149‑1 (MQ=255)
                                                                                                                               gAGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAAt                >  2:1753971/1‑149 (MQ=255)
                                                                                                                                aGCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATc               >  1:2075994/1‑149 (MQ=255)
                                                                                                                                 gCGAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCg              >  2:3009847/1‑149 (MQ=255)
                                                                                                                                   gAAGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAt            >  1:2567538/1‑149 (MQ=255)
                                                                                                                                    aaGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc           >  1:4116257/1‑149 (MQ=255)
                                                                                                                                    aaGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc           <  1:3913289/149‑1 (MQ=255)
                                                                                                                                    aaGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc           <  1:4703346/149‑1 (MQ=255)
                                                                                                                                    aaGAGATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtc           >  1:1193837/1‑149 (MQ=255)
                                                                                                                                     agagATCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtct          >  2:187184/1‑149 (MQ=255)
                                                                                                                                        gaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCgg       <  1:1978017/149‑1 (MQ=255)
                                                                                                                                        gaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCgg       >  2:1542593/1‑149 (MQ=255)
                                                                                                                                        gaTCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCgg       >  2:1801686/1‑149 (MQ=255)
                                                                                                                                          tCATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGaa     >  1:1128230/1‑149 (MQ=255)
                                                                                                                                           cATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGaaa    >  1:2689889/1‑149 (MQ=255)
                                                                                                                                           cATTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGaaa    >  2:241008/1‑149 (MQ=255)
                                                                                                                                            aTTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATCAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAAt   >  2:4790071/1‑149 (MQ=255)
                                                                                                                                            aTTTATTACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAAt   >  2:1398171/1‑149 (MQ=255)
                                                                                                                                             gttattaCGCCGATGGTCACCCGTTAACGCGTGTTTGACGCAGTTACGGCGCACCGACAATTGGTGACAGTCCGCGTGATTTCCGCGCGGTGCTGGTGCCGAGCGACGCTAAAGTCGGCCCGTTCGGGGCGAATTCGACCTCGGAAATc  <  2:2467898/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATC  >  CP009273/3017139‑3017428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑