Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 238,195 G→T 100% V532V (GTC→GTA)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273238,1950GT98.9% 941.6 / 0.6 276V532V (GTC→GTA) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (116/157);  total (116/160)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

GTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCG  >  CP009273/238051‑238340
                                                                                                                                                |                                                                                                                                                 
    cggTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                           <  1:764816/148‑1 (MQ=255)
    aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                           <  1:4170239/149‑1 (MQ=255)
    aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                           >  2:3076776/1‑149 (MQ=255)
    aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                           >  2:4265094/1‑149 (MQ=255)
    aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                           <  2:4389374/149‑1 (MQ=255)
    aGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCAtt                                                                                                                                           >  2:685714/1‑149 (MQ=255)
     ggTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTg                                                                                                                                          <  2:308433/149‑1 (MQ=255)
       tGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGtt                                                                                                                                        <  2:1672625/149‑1 (MQ=255)
         cTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttct                                                                                                                                      <  2:2711664/149‑1 (MQ=255)
         cTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttct                                                                                                                                      <  2:3414172/149‑1 (MQ=255)
         cTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttct                                                                                                                                      <  2:319458/149‑1 (MQ=255)
          tGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttctt                                                                                                                                     <  2:2985033/149‑1 (MQ=255)
          tGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttctt                                                                                                                                     >  1:2608561/1‑149 (MQ=255)
           gATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGttcttc                                                                                                                                    <  2:716261/149‑1 (MQ=255)
            aTAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcg                                                                                                                                   >  1:3749019/1‑149 (MQ=255)
            aTAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcg                                                                                                                                   >  2:3711226/1‑149 (MQ=255)
             tagtagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGGTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcgc                                                                                                                                  <  2:1614497/149‑1 (MQ=255)
              agtagCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTcgcc                                                                                                                                 >  1:2251178/1‑149 (MQ=255)
                  gCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCtt                                                                                                                             >  2:2518095/1‑149 (MQ=255)
                      ttAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAt                                                                                                                         <  2:80770/149‑1 (MQ=255)
                      ttAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAt                                                                                                                         <  2:3962283/149‑1 (MQ=255)
                       tAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAtc                                                                                                                        >  2:4780803/1‑149 (MQ=255)
                        aGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAtct                                                                                                                       <  1:4160123/149‑1 (MQ=255)
                         gTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAtctc                                                                                                                      <  1:639028/149‑1 (MQ=255)
                         gTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCAtctc                                                                                                                      >  1:1568870/1‑149 (MQ=255)
                          tGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATctct                                                                                                                     <  2:1906005/149‑1 (MQ=255)
                           ggCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCTATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCtt                                                                                                                    <  1:3250856/149‑1 (MQ=255)
                            gCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTc                                                                                                                   <  1:3853296/149‑1 (MQ=255)
                            gCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTc                                                                                                                   <  1:4023554/149‑1 (MQ=255)
                             cATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTcc                                                                                                                  <  1:2481139/149‑1 (MQ=255)
                                 gCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCa                                                                                                              >  2:257317/1‑149 (MQ=255)
                                  ccGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCAc                                                                                                             >  1:4578311/1‑149 (MQ=255)
                                    ggTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGt                                                                                                           <  1:528100/149‑1 (MQ=255)
                                           gTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATg                                                                                                    <  1:1979808/149‑1 (MQ=255)
                                           gTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATg                                                                                                    <  1:2262355/149‑1 (MQ=255)
                                              ctgctgGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCa                                                                                                 <  2:2260300/149‑1 (MQ=255)
                                                  tgGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGa                                                                                             >  2:2443053/1‑149 (MQ=255)
                                                    gTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAAt                                                                                           >  2:1347831/1‑149 (MQ=255)
                                                     ttAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATc                                                                                          <  1:1322421/149‑1 (MQ=255)
                                                     ttAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATc                                                                                          <  2:2859509/149‑1 (MQ=255)
                                                           cgcgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCt                                                                                    <  2:1260420/149‑1 (MQ=255)
                                                            gcgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCtt                                                                                   <  1:2423753/149‑1 (MQ=255)
                                                            gcgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCtt                                                                                   <  2:4635738/149‑1 (MQ=255)
                                                             cgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTg                                                                                  <  2:280901/149‑1 (MQ=255)
                                                             cgcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTg                                                                                  <  2:2913129/149‑1 (MQ=255)
                                                              gcgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGt                                                                                 <  1:2941766/149‑1 (MQ=255)
                                                               cgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTc                                                                                <  2:3800853/149‑1 (MQ=255)
                                                               cgTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTc                                                                                <  1:945318/149‑1 (MQ=255)
                                                                 tCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCg                                                                              >  1:2093381/1‑149 (MQ=255)
                                                                 tCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCg                                                                              >  2:2143980/1‑149 (MQ=255)
                                                                   aGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGaa                                                                            >  1:2863852/1‑149 (MQ=255)
                                                                    ggCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAg                                                                           <  1:1895824/149‑1 (MQ=255)
                                                                     gCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGa                                                                          <  2:3528380/149‑1 (MQ=255)
                                                                       ccagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatc                                                                        <  1:3738385/149‑1 (MQ=255)
                                                                       ccagccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatc                                                                        <  1:3579762/149‑1 (MQ=255)
                                                                          gccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACACCAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcatc                                                                     >  2:4521915/1‑149 (MQ=255)
                                                                          gccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGatcatc                                                                     >  2:4449296/1‑149 (MQ=255)
                                                                           ccagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGAtcatca                                                                    <  2:1465489/149‑1 (MQ=255)
                                                                            cagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATcatcat                                                                   >  2:1310260/1‑149 (MQ=255)
                                                                            cagAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATcatcat                                                                   >  1:2061468/1‑149 (MQ=255)
                                                                              gAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgc                                                                 >  2:2266785/1‑149 (MQ=255)
                                                                              gAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgc                                                                 >  1:1671608/1‑149 (MQ=255)
                                                                               aaGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgct                                                                <  2:788651/149‑1 (MQ=255)
                                                                                aGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgctg                                                               >  1:4079275/1‑149 (MQ=255)
                                                                                aGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCAtgctg                                                               >  1:351087/1‑149 (MQ=255)
                                                                                   tGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCgg                                                            <  1:4363383/149‑1 (MQ=255)
                                                                                   tGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCgg                                                            >  1:2728072/1‑149 (MQ=255)
                                                                                   tGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCgg                                                            >  1:335332/1‑149 (MQ=255)
                                                                                     cGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGt                                                          <  1:2553199/149‑1 (MQ=255)
                                                                                      gAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTc                                                         >  1:235127/1‑149 (MQ=255)
                                                                                          tttGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGaa                                                     <  2:4766522/149‑1 (MQ=255)
                                                                                            tgttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATg                                                   <  2:3132033/149‑1 (MQ=255)
                                                                                            tgttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATg                                                   <  1:1185208/149‑1 (MQ=255)
                                                                                             gttgCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGt                                                  <  1:4747838/149‑1 (MQ=255)
                                                                                                     cGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                          <  1:3976183/149‑1 (MQ=255)
                                                                                                     cGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCt                                          >  1:3446023/1‑149 (MQ=255)
                                                                                                      gACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCtt                                         >  2:1921649/1‑149 (MQ=255)
                                                                                                       aCGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTc                                        >  1:4498218/1‑149 (MQ=255)
                                                                                                       aCGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTc                                        >  1:3950709/1‑149 (MQ=255)
                                                                                                       aCGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTc                                        <  1:2132741/149‑1 (MQ=255)
                                                                                                          tGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAAc                                     <  1:4312686/149‑1 (MQ=255)
                                                                                                           gACCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACg                                    >  2:3877523/1‑149 (MQ=255)
                                                                                                            aCCGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACgg                                   <  1:3544049/149‑1 (MQ=255)
                                                                                                             ccGATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGt                                  <  2:4005769/149‑1 (MQ=255)
                                                                                                               gATATGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGa                                <  2:2612010/149‑1 (MQ=255)
                                                                                                                  atGTTTGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATgg                             >  2:3898923/1‑149 (MQ=255)
                                                                                                                     tttGAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCaa                          <  1:3167984/149‑1 (MQ=255)
                                                                                                                        gAACAACAGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCg                       <  2:3744481/149‑1 (MQ=255)
                                                                                                                          acaacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCACTCGGt                     >  1:288626/1‑149 (MQ=255)
                                                                                                                           caacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTg                    <  2:2172585/149‑1 (MQ=255)
                                                                                                                           caacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTg                    <  1:2024926/149‑1 (MQ=255)
                                                                                                                            aacaGTTTATCGACCGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgc                   <  1:312914/149‑1 (MQ=255)
                                                                                                                            aacaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTgc                   >  1:1051867/1‑149 (MQ=255)
                                                                                                                             acaGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCAAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGACCCTTCAACGGTGATGGCAATCGGTgcg                  >  2:176681/1‑149 (MQ=255)
                                                                                                                               aGTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGcc                >  2:1058430/1‑149 (MQ=255)
                                                                                                                                gTTTATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGccc               <  2:3809524/149‑1 (MQ=255)
                                                                                                                                 tttATCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCt              <  2:1553477/149‑1 (MQ=255)
                                                                                                                                    aTCGAACGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGt           >  2:532331/1‑149 (MQ=255)
                                                                                                                                        aaCGCGTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGc       <  2:3355080/149‑1 (MQ=255)
                                                                                                                                            cgTTTACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCAcgc   >  1:854423/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GTTCAGGTGCTGATAGTAGCGTTTAGTGGCATCGCCGGTTGGCGTGCTGCTGGTTAAACCGCGCGTCAGGCCCAGCCAGAAGCTGCGAACTTTGTTGCTACCGACGTGACCGATATGTTTGAACAACAGTTTATCGAACGCGTTGACGTCATTGTTCTTCGCCGCTTCCATCTCTTCCAGCACGTACGGATGGCAACGAATCGCTCCTTGTCCGAAGATCATCATGCTGCGGGTCAGAATGTTAGCCCCTTCAACGGTGATGGCAATCGGTGCGCCCTGGTAAGCACGCG  >  CP009273/238051‑238340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑