Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,978 A→T 5.3% L163* (TTG→TAG)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,9780AT 5.3% 924.3 / 31.1 282L163* (TTG→TAG) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base A (132/135);  new base T (7/8);  total (139/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 293 total aligned reads displayed.

AACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACC  >  CP009273/3030831‑3031126
                                                                                                                                                   |                                                                                                                                                    
aaCCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTCATGTGTCGCCTGGCaa                                                                                                                                                     >  2:3322050/1‑149 (MQ=255)
  ccAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAACTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAAt                                                                                                                                                   <  1:3362925/149‑1 (MQ=255)
  ccAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCatat                                                                                                                                                   <  2:4066576/149‑1 (MQ=255)
  ccAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAAt                                                                                                                                                   <  2:2510100/149‑1 (MQ=255)
    aGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCATACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATgg                                                                                                                                                 >  1:3600305/1‑149 (MQ=255)
    aGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATgg                                                                                                                                                 <  1:1653886/149‑1 (MQ=255)
       ccTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGa                                                                                                                                              <  1:2644118/149‑1 (MQ=255)
       ccTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGa                                                                                                                                              <  1:154636/149‑1 (MQ=255)
       ccTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGa                                                                                                                                              >  2:2500234/1‑149 (MQ=255)
         tGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATc                                                                                                                                            >  2:2659901/1‑149 (MQ=255)
          gATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCg                                                                                                                                           >  1:1167816/1‑149 (MQ=255)
             cgcgCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTa                                                                                                                                        <  2:1083092/149‑1 (MQ=255)
              gcgcAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTaa                                                                                                                                       <  2:1474626/149‑1 (MQ=255)
              gcgcAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTaa                                                                                                                                       >  1:4789456/1‑149 (MQ=255)
               cgcAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAAc                                                                                                                                      <  2:3938165/149‑1 (MQ=255)
                  aaaaaGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAAt                                                                                                                                   <  2:3849992/149‑1 (MQ=255)
                  aaaaaGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAAt                                                                                                                                   >  1:202443/1‑149 (MQ=255)
                   aaaaGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATa                                                                                                                                  <  2:444136/149‑1 (MQ=255)
                     aaGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAAc                                                                                                                                <  1:1131891/149‑1 (MQ=255)
                           cgcAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGAt                                                                                                                          <  2:2167023/149‑1 (MQ=255)
                           cgcAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGAt                                                                                                                          <  1:3917827/149‑1 (MQ=255)
                            gcAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATg                                                                                                                         >  1:481639/1‑149 (MQ=255)
                                  ccAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAAc                                                                                                                   <  1:218480/149‑1 (MQ=255)
                                  ccAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAAc                                                                                                                   <  1:3727242/149‑1 (MQ=255)
                                    aGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCTAATGGTGATCGGTAACAATAACCGGGATGCCCAAcga                                                                                                                 >  2:1716599/1‑149 (MQ=255)
                                        tCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAAcgagcga                                                                                                             <  2:4459569/149‑1 (MQ=255)
                                           gATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGagcgagcg                                                                                                          <  1:312144/149‑1 (MQ=255)
                                           gATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGagcgagcg                                                                                                          >  2:4778241/1‑149 (MQ=255)
                                            aTAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGt                                                                                                         >  2:3853280/1‑149 (MQ=255)
                                               aaaCGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCt                                                                                                      <  1:3120773/149‑1 (MQ=255)
                                               aaaCGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCt                                                                                                      >  1:1679921/1‑149 (MQ=255)
                                                aaCGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTc                                                                                                     <  2:4333733/149‑1 (MQ=255)
                                                       cacCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAAccccc                                                                                              <  2:2866617/149‑1 (MQ=255)
                                                              gCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATggg                                                                                       <  1:1373746/149‑1 (MQ=255)
                                                               ccTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGgga                                                                                      <  1:582175/149‑1 (MQ=255)
                                                                 tGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGggagg                                                                                    >  2:805869/1‑149 (MQ=255)
                                                                 tGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGggagg                                                                                    >  2:1995365/1‑149 (MQ=255)
                                                                   ccAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCTAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGaa                                                                                  >  2:4011416/1‑149 (MQ=255)
                                                                      gTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATa                                                                               >  1:4111890/1‑149 (MQ=255)
                                                                      gTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATa                                                                               >  1:2696455/1‑149 (MQ=255)
                                                                      gTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATa                                                                               >  1:4469091/1‑149 (MQ=255)
                                                                        gATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCTACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATAcc                                                                             >  1:2361650/1‑149 (MQ=255)
                                                                        gATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATAcc                                                                             >  1:4581465/1‑149 (MQ=255)
                                                                         aTTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCg                                                                            >  1:3526930/1‑149 (MQ=255)
                                                                           ttCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGtt                                                                          <  2:3088694/149‑1 (MQ=255)
                                                                           ttCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGtt                                                                          <  1:3747009/149‑1 (MQ=255)
                                                                            tCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTa                                                                         <  1:702705/149‑1 (MQ=255)
                                                                             cGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCTAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTAt                                                                        <  1:3973745/149‑1 (MQ=255)
                                                                                cGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCa                                                                     <  2:4587454/149‑1 (MQ=255)
                                                                                   gAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCg                                                                  >  2:4662657/1‑149 (MQ=255)
                                                                                    aaaTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGt                                                                 >  1:2214663/1‑149 (MQ=255)
                                                                                     aaTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCTAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCgag                                                                >  2:421070/1‑147 (MQ=255)
                                                                                      aTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGa                                                               >  1:4144386/1‑149 (MQ=255)
                                                                                      aTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGa                                                               <  2:653394/149‑1 (MQ=255)
                                                                                       ttACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGAc                                                              <  2:70268/149‑1 (MQ=255)
                                                                                       ttACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGAc                                                              <  2:2420563/149‑1 (MQ=255)
                                                                                       ttACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGAc                                                              <  2:2507412/149‑1 (MQ=255)
                                                                                            gTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTa                                                         >  2:2670649/1‑149 (MQ=255)
                                                                                             tCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTaa                                                        <  1:819334/149‑1 (MQ=255)
                                                                                              cgcgCAAGTTAGGGTTACTGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCTACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAAc                                                       <  2:1169938/149‑1 (MQ=255)
                                                                                              cgcgCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAAc                                                       <  2:3245746/149‑1 (MQ=255)
                                                                                                 gcAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTACTGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgc                                                    >  2:3597201/1‑149 (MQ=255)
                                                                                                 gcAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgc                                                    >  1:4330928/1‑149 (MQ=255)
                                                                                                 gcAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgc                                                    <  1:3307767/149‑1 (MQ=255)
                                                                                                   aaGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCTAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgcgc                                                  >  1:3995520/1‑149 (MQ=255)
                                                                                                   aaGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgcgc                                                  <  2:3483638/149‑1 (MQ=255)
                                                                                                     gTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgcgcgc                                                >  2:232278/1‑149 (MQ=255)
                                                                                                        aGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCAc                                             >  2:1318077/1‑149 (MQ=255)
                                                                                                           gTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACggg                                          >  2:3375803/1‑149 (MQ=255)
                                                                                                             tAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCa                                        >  1:3440022/1‑149 (MQ=255)
                                                                                                              aaTGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCAt                                       <  2:5740/149‑1 (MQ=255)
                                                                                                              aaTGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCAt                                       <  1:997576/149‑1 (MQ=255)
                                                                                                                 gATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATggg                                    >  1:516645/1‑149 (MQ=255)
                                                                                                                  aTCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGc                                   >  1:2212471/1‑149 (MQ=255)
                                                                                                                   tCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATACCCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGcc                                  <  2:2938336/149‑1 (MQ=255)
                                                                                                                   tCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGcc                                  <  2:195863/149‑1 (MQ=255)
                                                                                                                   tCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGcc                                  <  2:1796672/149‑1 (MQ=255)
                                                                                                                   tCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGcc                                  <  1:2250415/149‑1 (MQ=255)
                                                                                                                   tCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGcc                                  <  1:1280474/149‑1 (MQ=255)
                                                                                                                    cGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCt                                 >  2:2988614/1‑149 (MQ=255)
                                                                                                                    cGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCt                                 >  1:688279/1‑149 (MQ=255)
                                                                                                                    cGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCt                                 <  2:3663722/149‑1 (MQ=255)
                                                                                                                    cGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCt                                 <  2:1578694/149‑1 (MQ=255)
                                                                                                                     gCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCAGGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTg                                >  2:2615994/1‑149 (MQ=255)
                                                                                                                      cTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGa                               >  2:3870496/1‑149 (MQ=255)
                                                                                                                       ttCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGAt                              >  1:298950/1‑149 (MQ=255)
                                                                                                                          cGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGa                           <  1:2268104/149‑1 (MQ=255)
                                                                                                                            cTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGAcc                         <  1:2500345/149‑1 (MQ=255)
                                                                                                                              gCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGTGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCAc                       >  2:4373942/1‑149 (MQ=255)
                                                                                                                                 ggTAATGTGTCGCCTGGCTAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTc                    >  1:1041805/1‑149 (MQ=255)
                                                                                                                                    aaTGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCgg                 <  1:3047297/149‑1 (MQ=255)
                                                                                                                                          tCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTaa           <  2:1220616/149‑1 (MQ=255)
                                                                                                                                           cGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAg          >  1:903888/1‑149 (MQ=255)
                                                                                                                                               tGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGt      >  2:4370699/1‑149 (MQ=255)
                                                                                                                                                ggCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTa     <  1:1742213/149‑1 (MQ=255)
                                                                                                                                                 gCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTaa    <  1:3580087/149‑1 (MQ=255)
                                                                                                                                                  cAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAAc   <  2:3154253/149‑1 (MQ=255)
                                                                                                                                                   aaaTGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAAcc  >  1:1089664/1‑149 (MQ=255)
                                                                                                                                                   aaaTGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAAcc  >  1:2471664/1‑149 (MQ=255)
                                                                                                                                                   aaaTGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAAcc  >  1:38228/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACC  >  CP009273/3030831‑3031126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑