Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,618 T→A 100% V185E (GTG→GAG)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,6180TA99.7% 1034.7 / ‑4.8 298V185E (GTG→GAG) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (147/150);  total (147/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 298 total aligned reads displayed.

AGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATC  >  CP009273/3080472‑3080763
                                                                                                                                                  |                                                                                                                                                 
aGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGc                                                                                                                                                 <  1:4206576/149‑1 (MQ=255)
aGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGc                                                                                                                                                 >  2:1553622/1‑149 (MQ=255)
 gCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCt                                                                                                                                                >  1:3235456/1‑149 (MQ=255)
 gCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCt                                                                                                                                                >  1:2552218/1‑149 (MQ=255)
 gCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCt                                                                                                                                                >  2:1134948/1‑149 (MQ=255)
  cacctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCtt                                                                                                                                               >  2:4711076/1‑149 (MQ=255)
   acctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCttt                                                                                                                                              <  2:4572819/149‑1 (MQ=255)
   acctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCttt                                                                                                                                              <  2:4130431/149‑1 (MQ=255)
    cctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTc                                                                                                                                             <  2:2761774/149‑1 (MQ=255)
       atcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCAc                                                                                                                                          >  2:2250472/1‑149 (MQ=255)
       atcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCAc                                                                                                                                          >  1:4400794/1‑149 (MQ=255)
        tcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACt                                                                                                                                         >  2:901404/1‑149 (MQ=255)
        tcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACt                                                                                                                                         <  1:681399/149‑1 (MQ=255)
            ctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGc                                                                                                                                     >  1:575462/1‑149 (MQ=255)
            ctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGc                                                                                                                                     >  2:1563774/1‑149 (MQ=255)
             tatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCa                                                                                                                                    >  2:1468877/1‑149 (MQ=255)
             tatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCa                                                                                                                                    >  1:4391726/1‑149 (MQ=255)
                  acacCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTg                                                                                                                               >  1:908704/1‑149 (MQ=255)
                  acacCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTg                                                                                                                               >  1:1172015/1‑149 (MQ=255)
                    acCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGaa                                                                                                                             >  2:1707216/1‑149 (MQ=255)
                    acCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGaa                                                                                                                             >  2:1937014/1‑149 (MQ=255)
                        tCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAgaga                                                                                                                         >  1:2696496/1‑149 (MQ=255)
                            gTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGa                                                                                                                     <  1:2137354/149‑1 (MQ=255)
                             tACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAc                                                                                                                    <  1:1306055/149‑1 (MQ=255)
                                   gTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGaaa                                                                                                              <  1:1653866/149‑1 (MQ=255)
                                     cAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATc                                                                                                            >  2:3220655/1‑149 (MQ=255)
                                        gCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCt                                                                                                         <  1:1039513/149‑1 (MQ=255)
                                        gCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCt                                                                                                         <  2:3717400/149‑1 (MQ=255)
                                          tGAAGTGCGTAAACACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGc                                                                                                       <  2:3945508/149‑1 (MQ=255)
                                            aaGTGCTTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaa                                                                                                     <  1:3332373/149‑1 (MQ=255)
                                            aaGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaa                                                                                                     <  1:3511303/149‑1 (MQ=255)
                                             aGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaag                                                                                                    <  2:2331990/149‑1 (MQ=255)
                                               tGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaagaa                                                                                                  <  1:1430997/149‑1 (MQ=255)
                                                   tAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                              <  1:3875970/149‑1 (MQ=255)
                                                   tAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                              >  2:1789034/1‑149 (MQ=255)
                                                   tAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                              <  2:769901/149‑1 (MQ=255)
                                                   tAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                              <  2:1345226/149‑1 (MQ=255)
                                                   tAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                              >  1:54104/1‑149 (MQ=255)
                                                   tAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGGTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                              >  2:445473/1‑149 (MQ=255)
                                                     aaaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaagaag                                                                                                 <  1:2004532/144‑1 (MQ=255)
                                                        aCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATg                                                                                         >  2:3643640/1‑149 (MQ=255)
                                                        aCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATg                                                                                         >  2:818963/1‑149 (MQ=255)
                                                        aCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATg                                                                                         >  2:1254945/1‑149 (MQ=255)
                                                          ggCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGa                                                                                       >  1:2938044/1‑149 (MQ=255)
                                                           gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGaa                                                                                      >  1:4348423/1‑149 (MQ=255)
                                                           gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGaa                                                                                      <  1:4661612/149‑1 (MQ=255)
                                                               tctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAgaga                                                                                  <  2:4321793/149‑1 (MQ=255)
                                                               tctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAgaga                                                                                  <  2:3433470/149‑1 (MQ=255)
                                                                ctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGat                                                                                 <  1:3729927/149‑1 (MQ=255)
                                                                   ccGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGatcat                                                                              <  2:2043169/149‑1 (MQ=255)
                                                                   ccGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGatcat                                                                              <  2:2794088/149‑1 (MQ=255)
                                                                    cGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGatcatc                                                                             >  1:176187/1‑149 (MQ=255)
                                                                     gTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGAtcatca                                                                            >  1:656324/1‑149 (MQ=255)
                                                                      tGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCaa                                                                           >  2:42049/1‑149 (MQ=255)
                                                                      tGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCaa                                                                           <  1:1922380/149‑1 (MQ=255)
                                                                          tGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCa                                                                       <  2:570635/149‑1 (MQ=255)
                                                                          tGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCa                                                                       >  2:2927397/1‑149 (MQ=255)
                                                                            cgcCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAAt                                                                     <  1:2609305/149‑1 (MQ=255)
                                                                               ccGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCt                                                                  <  2:1977149/149‑1 (MQ=255)
                                                                                 ggACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGc                                                                <  1:2842428/149‑1 (MQ=255)
                                                                                 ggACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGc                                                                >  2:2842364/1‑149 (MQ=255)
                                                                                 ggACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGc                                                                <  1:449498/149‑1 (MQ=255)
                                                                                       gAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTCATGGAAGAGATCATCAAGCCAATTCTGCCCGCTg                                                          >  2:1116186/1‑149 (MQ=255)
                                                                                       gAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTg                                                          <  1:4710605/149‑1 (MQ=255)
                                                                                        aaaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGa                                                         >  1:3555429/1‑149 (MQ=255)
                                                                                         aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGaa                                                        >  2:1204373/1‑149 (MQ=255)
                                                                                         aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGaa                                                        >  2:3594733/1‑149 (MQ=255)
                                                                                         aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGaa                                                        >  1:3721206/1‑149 (MQ=255)
                                                                                            gCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATgg                                                     <  2:3080527/149‑1 (MQ=255)
                                                                                            gCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATgg                                                     <  1:2408423/149‑1 (MQ=255)
                                                                                            gCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATgg                                                     >  1:3165136/1‑149 (MQ=255)
                                                                                               aGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTg                                                  <  1:4016002/149‑1 (MQ=255)
                                                                                                ggTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGa                                                 <  1:2778766/149‑1 (MQ=255)
                                                                                                 gTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGAc                                                >  2:694713/1‑149 (MQ=255)
                                                                                                  tGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACt                                               >  1:996585/1‑149 (MQ=255)
                                                                                                  tGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACt                                               <  1:4197535/149‑1 (MQ=255)
                                                                                                   gACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACtt                                              <  2:3064506/149‑1 (MQ=255)
                                                                                                    aCTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTc                                             >  1:1357490/1‑149 (MQ=255)
                                                                                                     cTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCt                                            <  1:1494609/149‑1 (MQ=255)
                                                                                                       tttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGc                                          >  1:594555/1‑149 (MQ=255)
                                                                                                           aGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGccacc                                      >  1:1657933/1‑149 (MQ=255)
                                                                                                            gTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGATATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGccacca                                     >  2:3239906/1‑149 (MQ=255)
                                                                                                            gTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGccacca                                     >  2:2803833/1‑149 (MQ=255)
                                                                                                            gTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGccacca                                     >  1:3304497/1‑149 (MQ=255)
                                                                                                              aTGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCCATTCTGCCCGCTGAATGGCTGACTTCTGCCCCCaaa                                   >  2:3786376/1‑149 (MQ=255)
                                                                                                              aTGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCaaa                                   >  1:1594122/1‑149 (MQ=255)
                                                                                                               tGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAt                                  >  2:3839610/1‑149 (MQ=255)
                                                                                                                gacgacGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAtt                                 <  2:2673697/149‑1 (MQ=255)
                                                                                                                  cgacgGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAttct                               <  1:3649465/149‑1 (MQ=255)
                                                                                                                     cgGCATAATCGTTGGTATCGATGCTGTCGATCTGTACACTAATCACTCTGAAGTGTTCGACCAGAAATCGCTGCTAGAAGCGGTAATGGAAGAGATCATCTAGCAAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtca                            <  1:737224/149‑1 (MQ=255)
                                                                                                                       gCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtcatc                          <  2:1172015/149‑1 (MQ=255)
                                                                                                                          aaaTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAc                       <  1:3414326/149‑1 (MQ=255)
                                                                                                                            aTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAccc                     <  2:2466425/149‑1 (MQ=255)
                                                                                                                                ttGGTATCGATGCAGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGAcc                 <  2:1619734/149‑1 (MQ=255)
                                                                                                                                  ggTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCgg               >  1:624549/1‑149 (MQ=255)
                                                                                                                                    tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtc             <  2:2164453/149‑1 (MQ=255)
                                                                                                                                    tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtc             <  1:2252032/149‑1 (MQ=255)
                                                                                                                                       cGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt          <  2:3967491/149‑1 (MQ=255)
                                                                                                                                              gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt   >  1:3507121/1‑149 (MQ=255)
                                                                                                                                               tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc  <  1:953456/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATC  >  CP009273/3080472‑3080763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑