BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,082,252	T→A	5.5%	F204I (TTT→ATT)	galP →	D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,082,252	0	T	A	5.5%	854.1 / 29.7	272	F204I (TTT→ATT)	galP	D‑galactose transporter
Reads supporting (aligned to +/- strand): ref base T (114/142); new base A (8/7); total (123/149)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 282 total aligned reads displayed.

CCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTT  >  CP009273/3082104‑3082400

ccTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGtt                                                                                                                                                      <  1:1872344/149‑1 (MQ=255)

    ggTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTttgt                                                                                                                                                  <  2:2157268/149‑1 (MQ=255)

     gTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTttgtt                                                                                                                                                 <  2:4102640/149‑1 (MQ=255)

     gTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTttgtt                                                                                                                                                 >  1:2553133/1‑149 (MQ=255)

     gTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTttgtt                                                                                                                                                 >  1:4774805/1‑149 (MQ=255)

      tGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTttgttg                                                                                                                                                >  2:2800669/1‑149 (MQ=255)

       gCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGa                                                                                                                                               >  1:4530493/1‑149 (MQ=255)

         ttATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATg                                                                                                                                             >  1:3322215/1‑149 (MQ=255)

           aTCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGcc                                                                                                                                           <  1:3934269/149‑1 (MQ=255)

           aTCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGcc                                                                                                                                           <  1:3058500/149‑1 (MQ=255)

            tCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCg                                                                                                                                          >  1:1678995/1‑149 (MQ=255)

            tCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCg                                                                                                                                          <  1:1749101/149‑1 (MQ=255)

            tCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCg                                                                                                                                          >  1:4686548/1‑149 (MQ=255)

            tCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCg                                                                                                                                          >  1:2289111/1‑149 (MQ=255)

              tttCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGaa                                                                                                                                        <  2:2036005/149‑1 (MQ=255)

              tttCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGaa                                                                                                                                        >  1:2496890/1‑149 (MQ=255)

               ttCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAAc                                                                                                                                       >  1:3191940/1‑149 (MQ=255)

               ttCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAAc                                                                                                                                       >  2:1187246/1‑149 (MQ=255)

                  tGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAAcgcg                                                                                                                                    >  1:2118471/1‑149 (MQ=255)

                      aCCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGtgct                                                                                                                                <  2:3747188/149‑1 (MQ=255)

                       ccGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGtgctg                                                                                                                               >  1:219207/1‑149 (MQ=255)

                          ccTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTa                                                                                                                            >  1:932710/1‑149 (MQ=255)

                          ccTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTa                                                                                                                            >  2:1776982/1‑149 (MQ=255)

                           cTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTAc                                                                                                                           <  1:2266253/149‑1 (MQ=255)

                            ttCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACg                                                                                                                          >  2:4307006/1‑149 (MQ=255)

                               aGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCt                                                                                                                       <  2:1868719/149‑1 (MQ=255)

                                gCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTg                                                                                                                      >  1:4080984/1‑149 (MQ=255)

                                  tACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCg                                                                                                                    >  2:4297852/1‑149 (MQ=255)

                                  tACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCg                                                                                                                    >  2:4613952/1‑149 (MQ=255)

                                      ccGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGac                                                                                                                <  1:2292242/149‑1 (MQ=255)

                                        ggTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGacac                                                                                                              <  1:4000627/149‑1 (MQ=255)

                                           gCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAg                                                                                                           <  2:769456/149‑1 (MQ=255)

                                           gCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAg                                                                                                           <  1:4490092/149‑1 (MQ=255)

                                           gCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAg                                                                                                           <  1:3658405/149‑1 (MQ=255)

                                            cATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCGTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAgc                                                                                                          <  2:3441573/149‑1 (MQ=255)

                                             aTGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAgcg                                                                                                         >  2:46557/1‑149 (MQ=255)

                                              tGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAgcgc                                                                                                        >  1:1605708/1‑149 (MQ=255)

                                                 cgcTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGa                                                                                                     <  1:3553390/149‑1 (MQ=255)

                                                      gctGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCGGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGCTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGa                                                                                                <  2:674474/147‑1 (MQ=255)

                                                      gATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGa                                                                                                <  2:3496517/149‑1 (MQ=255)

                                                      gATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGa                                                                                                <  2:232201/149‑1 (MQ=255)

                                                       aTGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGaa                                                                                               <  2:1556600/149‑1 (MQ=255)

                                                        tGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGaaa                                                                                              <  1:1964472/149‑1 (MQ=255)

                                                        tGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGaaa                                                                                              <  1:4353873/149‑1 (MQ=255)

                                                           tGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAAcgc                                                                                           <  2:3446532/149‑1 (MQ=255)

                                                           tGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAAcgc                                                                                           <  2:1417048/149‑1 (MQ=255)

                                                              gtgtGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGaa                                                                                        <  1:88833/149‑1 (MQ=255)

                                                                gtgATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACt                                                                                      >  2:1325304/1‑149 (MQ=255)

                                                                 tgATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTg                                                                                     <  1:963676/149‑1 (MQ=255)

                                                                  gATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTgg                                                                                    <  2:3985741/149‑1 (MQ=255)

                                                                  gATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTgg                                                                                    <  1:1007425/149‑1 (MQ=255)

                                                                   aTTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGa                                                                                   >  2:2742497/1‑149 (MQ=255)

                                                                    ttATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGAt                                                                                  <  2:4244131/149‑1 (MQ=255)

                                                                        catcCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGaaa                                                                              >  2:1715758/1‑149 (MQ=255)

                                                                        catcCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGaaa                                                                              <  1:424791/149‑1 (MQ=255)

                                                                         atcCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAAt                                                                             >  2:2430099/1‑149 (MQ=255)

                                                                          tcCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATc                                                                            <  1:2582651/149‑1 (MQ=255)

                                                                             cGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGt                                                                         >  2:2712549/1‑149 (MQ=255)

                                                                              ggCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTg                                                                        >  2:4163661/1‑149 (MQ=255)

                                                                              ggCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTg                                                                        >  1:2105579/1‑149 (MQ=255)

                                                                               gCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGa                                                                       >  2:3896337/1‑149 (MQ=255)

                                                                               gCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGa                                                                       >  2:4770076/1‑149 (MQ=255)

                                                                                 aaTTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGaaa                                                                     <  1:179825/149‑1 (MQ=255)

                                                                                 aaTTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGaaa                                                                     >  2:614724/1‑149 (MQ=255)

                                                                                   ttttGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGt                                                                   <  1:2091423/149‑1 (MQ=255)

                                                                                   ttttGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGt                                                                   <  2:4062869/149‑1 (MQ=255)

                                                                                   ttttGCTGCTGAATGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTAGCCGCCAAACGCCGTGTTGTAGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGACGCGAAACGCGAACTGCATGACATCCGTGAAAGt                                                                   >  2:3683004/1‑149 (MQ=255)

                                                                                        ctgctgATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCa                                                              >  1:4308321/1‑149 (MQ=255)

                                                                                          gctgATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGACACGCGAACTGGATGAAATCCGTGAAAGTGTGCAgg                                                            >  1:2949511/1‑149 (MQ=255)

                                                                                           ctgATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGt                                                           <  2:2566036/149‑1 (MQ=255)

                                                                                               ttGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTaaa                                                       >  2:575745/1‑149 (MQ=255)

                                                                                               ttGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTaaa                                                       <  1:3066038/149‑1 (MQ=255)

                                                                                               ttGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTaaa                                                       >  2:2967973/1‑149 (MQ=255)

                                                                                                 ggTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACa                                                     <  1:2771455/149‑1 (MQ=255)

                                                                                                 ggTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACa                                                     >  2:1936953/1‑149 (MQ=255)

                                                                                                  gtgtCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACag                                                    <  2:3075125/149‑1 (MQ=255)

                                                                                                  gtgtCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACag                                                    <  2:684848/149‑1 (MQ=255)

                                                                                                   tgtCTTCGTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACaga                                                   <  1:4560368/149‑1 (MQ=255)

                                                                                                    gtCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACagag                                                  <  2:1448646/149‑1 (MQ=255)

                                                                                                      cttcttCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTg                                                <  1:671951/149‑1 (MQ=255)

                                                                                                          ttcCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTg                                            <  1:1127312/149‑1 (MQ=255)

                                                                                                          ttcCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTg                                            <  1:3506865/149‑1 (MQ=255)

                                                                                                             cTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGgc                                         <  1:284566/149‑1 (MQ=255)

                                                                                                                   gACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGtt                                   <  2:3267126/149‑1 (MQ=255)

                                                                                                                          cACGTTGGTATGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAACTCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAagag                            >  2:884966/1‑149 (MQ=255)

                                                                                                                             gTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAAc                         <  1:4447769/149‑1 (MQ=255)

                                                                                                                              ttGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACa                        <  1:2898224/149‑1 (MQ=255)

                                                                                                                                  tttGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCaa                    >  1:325936/1‑149 (MQ=255)

                                                                                                                                  tttGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCaa                    >  1:2718708/1‑149 (MQ=255)

                                                                                                                                  tttGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCaa                    >  2:2912388/1‑149 (MQ=255)

                                                                                                                                   ttGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAAc                   <  2:507566/149‑1 (MQ=255)

                                                                                                                                         ccAAACGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTccgc             <  1:1423373/149‑1 (MQ=255)

                                                                                                                                          cAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTccgcc            <  1:3408696/149‑1 (MQ=255)

                                                                                                                                           aaaCGCCGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTccgccg           >  1:4100592/1‑149 (MQ=255)

                                                                                                                                                ccGTATTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCgg      <  2:1259890/149‑1 (MQ=255)

                                                                                                                                                 cGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGgt     <  2:26887/149‑1 (MQ=255)

                                                                                                                                                 cGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGgt     >  1:1477620/1‑149 (MQ=255)

                                                                                                                                                  gTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGgtg    <  1:2626782/149‑1 (MQ=255)

                                                                                                                                                  gTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGgtg    >  1:1240188/1‑149 (MQ=255)

                                                                                                                                                    tttGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGtt  <  1:667410/149‑1 (MQ=255)

CCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTT  >  CP009273/3082104‑3082400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`