Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,088,065 C→A 100% V125V (GTG→GTT)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,088,0650CA98.8% 1140.1 / 2.8 338V125V (GTG→GTT) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (173/161);  total (176/162)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 338 total aligned reads displayed.

TCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATC  >  CP009273/3087919‑3088210
                                                                                                                                                  |                                                                                                                                                 
tCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAAc                                                                                                                                                 <  2:2116598/149‑1 (MQ=255)
  ccGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCa                                                                                                                                               >  1:102364/1‑149 (MQ=255)
  ccGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCa                                                                                                                                               >  1:3107173/1‑149 (MQ=255)
  ccGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCa                                                                                                                                               >  1:2494618/1‑149 (MQ=255)
      tgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAAt                                                                                                                                           >  2:4437648/1‑149 (MQ=255)
         tgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAg                                                                                                                                        >  2:2043800/1‑149 (MQ=255)
           gATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGc                                                                                                                                      <  1:590647/149‑1 (MQ=255)
            aTCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGcc                                                                                                                                     >  1:2366836/1‑149 (MQ=255)
             tCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCa                                                                                                                                    <  2:2495080/149‑1 (MQ=255)
              cAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAt                                                                                                                                   >  1:2354437/1‑149 (MQ=255)
              cAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAt                                                                                                                                   >  2:4357393/1‑149 (MQ=255)
               aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAtt                                                                                                                                  >  2:4021336/1‑149 (MQ=255)
               aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAtt                                                                                                                                  <  2:2333607/149‑1 (MQ=255)
                aaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                 >  2:44230/1‑149 (MQ=255)
                  cATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtctc                                                                                                                               <  1:1461706/149‑1 (MQ=255)
                      gctggctgGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGctc                                                                                                                           <  1:3756256/149‑1 (MQ=255)
                            tggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGt                                                                                                                     >  1:3827462/1‑149 (MQ=255)
                               cATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAAt                                                                                                                  <  2:4614741/149‑1 (MQ=255)
                                 tAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTc                                                                                                                >  1:3409396/1‑149 (MQ=255)
                                  agagATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                                                                                               <  2:2465896/149‑1 (MQ=255)
                                  agagATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                                                                                               <  2:4277669/149‑1 (MQ=255)
                                  agagATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                                                                                               >  2:4145578/1‑149 (MQ=255)
                                  agagATATTCAACAGGATCTTCCAGAGTCAGAATATGCGCATAGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                                                                                               <  1:2697074/149‑1 (MQ=255)
                                   gagaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCg                                                                                                              >  2:4685357/1‑149 (MQ=255)
                                    agaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCgg                                                                                                             >  1:1769716/1‑149 (MQ=255)
                                     gaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGc                                                                                                            >  2:1190166/1‑149 (MQ=255)
                                     gaTATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGc                                                                                                            <  2:3997900/149‑1 (MQ=255)
                                      atatTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCa                                                                                                           >  2:3431113/1‑149 (MQ=255)
                                      atatTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCa                                                                                                           <  1:1075261/149‑1 (MQ=255)
                                      atatTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCa                                                                                                           >  2:1640483/1‑149 (MQ=255)
                                       tatTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCaa                                                                                                          <  1:511288/149‑1 (MQ=255)
                                           cAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATAcc                                                                                                      <  1:4591376/149‑1 (MQ=255)
                                            aaCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                                                                                     >  1:1328538/1‑149 (MQ=255)
                                             aCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                                                                                    <  2:1339785/149‑1 (MQ=255)
                                              cAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGtt                                                                                                   <  1:4052998/149‑1 (MQ=255)
                                               aGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCTCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                  <  2:58744/149‑1 (MQ=255)
                                               aGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                  <  1:3618123/149‑1 (MQ=255)
                                                   tCTTCCAGCGTCAGAATATGCGCATCGGCCTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgc                                                                                              <  2:2579174/149‑1 (MQ=255)
                                                       ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCa                                                                                          >  2:2712155/1‑149 (MQ=255)
                                                       ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTGGAGTAATTCCGGCAATACCGTTGGTGCGCCa                                                                                          >  2:4701425/1‑149 (MQ=255)
                                                        cAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCaa                                                                                         <  2:4572977/149‑1 (MQ=255)
                                                              cAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGtt                                                                                   <  2:2961447/149‑1 (MQ=255)
                                                                 aaTATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGa                                                                                <  2:3316880/149‑1 (MQ=255)
                                                                  atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAg                                                                               >  1:4293925/1‑149 (MQ=255)
                                                                    atGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCt                                                                             <  1:94548/149‑1 (MQ=255)
                                                                     tGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTg                                                                            >  1:4747024/1‑149 (MQ=255)
                                                                        gcATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCgg                                                                         <  1:2183575/149‑1 (MQ=255)
                                                                        gcATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCACCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCgg                                                                         <  1:434380/149‑1 (MQ=255)
                                                                           tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCa                                                                      >  1:3322244/1‑149 (MQ=255)
                                                                           tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCa                                                                      >  2:3792934/1‑149 (MQ=255)
                                                                             ggCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGt                                                                    <  1:2252117/149‑1 (MQ=255)
                                                                               cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGc                                                                  <  1:650750/149‑1 (MQ=255)
                                                                                aTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                                                 >  2:1011186/1‑149 (MQ=255)
                                                                                 tGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                >  1:2751161/1‑149 (MQ=255)
                                                                                 tGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                >  1:1908967/1‑149 (MQ=255)
                                                                                  gTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGaa                                                               >  1:2921499/1‑149 (MQ=255)
                                                                                    tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAgg                                                             <  2:3242325/149‑1 (MQ=255)
                                                                                     gattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                            >  2:209363/1‑149 (MQ=255)
                                                                                     gattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                            <  1:3573803/149‑1 (MQ=255)
                                                                                     gattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                            <  2:873018/149‑1 (MQ=255)
                                                                                      attgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTa                                                           >  1:1004652/1‑149 (MQ=255)
                                                                                        tgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                                         <  1:3308672/149‑1 (MQ=255)
                                                                                         gagaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCACCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACa                                                        <  2:2377885/149‑1 (MQ=255)
                                                                                           gaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGc                                                      <  1:762846/149‑1 (MQ=255)
                                                                                           gaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGc                                                      <  1:1268047/149‑1 (MQ=255)
                                                                                            aTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGcc                                                     >  2:3938162/1‑149 (MQ=255)
                                                                                                 cAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTCACAGCCGTAAc                                                >  2:615976/1‑149 (MQ=255)
                                                                                                  aaCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACg                                               >  1:3163684/1‑149 (MQ=255)
                                                                                                   aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGc                                              >  1:1644418/1‑149 (MQ=255)
                                                                                                   aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGc                                              >  1:4700513/1‑149 (MQ=255)
                                                                                                      aTCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAg                                           >  2:2254859/1‑149 (MQ=255)
                                                                                                        cgccgcCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCg                                         >  2:3125808/1‑149 (MQ=255)
                                                                                                         gccgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGa                                        <  1:1655924/149‑1 (MQ=255)
                                                                                                         gccgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGa                                        >  2:1782557/1‑149 (MQ=255)
                                                                                                           cgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaaa                                      <  2:2769015/149‑1 (MQ=255)
                                                                                                           cgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaaa                                      <  2:1545897/149‑1 (MQ=255)
                                                                                                             ccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatg                                    >  1:1706148/1‑149 (MQ=255)
                                                                                                               aGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcc                                  <  2:4338827/149‑1 (MQ=255)
                                                                                                                gCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGACGGTAACAGCCGTAACGCCAGCGAAatgcca                                 >  2:1745338/1‑149 (MQ=255)
                                                                                                                gCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcca                                 >  1:3838890/1‑149 (MQ=255)
                                                                                                                gCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcca                                 >  1:891820/1‑149 (MQ=255)
                                                                                                                gCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcca                                 <  1:2567558/149‑1 (MQ=255)
                                                                                                                 cGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccat                                <  2:2999202/149‑1 (MQ=255)
                                                                                                                  gTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatg                               <  1:2654645/149‑1 (MQ=255)
                                                                                                                   tGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgc                              <  1:1820138/149‑1 (MQ=255)
                                                                                                                      tAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGt                           <  2:2542580/149‑1 (MQ=255)
                                                                                                                      tAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGt                           <  1:262502/149‑1 (MQ=255)
                                                                                                                      tAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGt                           <  2:2823282/149‑1 (MQ=255)
                                                                                                                       aGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGtt                          >  1:1746771/1‑149 (MQ=255)
                                                                                                                          tttGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgcg                       >  1:4170037/1‑149 (MQ=255)
                                                                                                                          tttGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgcg                       >  1:2800864/1‑149 (MQ=255)
                                                                                                                                   ccccGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATgcgc              >  2:3153682/1‑149 (MQ=255)
                                                                                                                                    cccGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCt             <  1:2285492/149‑1 (MQ=255)
                                                                                                                                       gTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGcc          <  1:1413660/149‑1 (MQ=255)
                                                                                                                                       gTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGcc          <  1:44230/149‑1 (MQ=255)
                                                                                                                                         cGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCAc        >  2:3602584/1‑149 (MQ=255)
                                                                                                                                         cGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCAc        <  2:4459213/149‑1 (MQ=255)
                                                                                                                                          gCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACg       >  2:1266759/1‑149 (MQ=255)
                                                                                                                                           cccccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGc      <  1:579390/149‑1 (MQ=255)
                                                                                                                                              ccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAAt   <  2:1487694/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATC  >  CP009273/3087919‑3088210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑