Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,111,434 C→A 100% A346A (GCG→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,111,4340CA100.0% 917.7 / ‑6.7 270A346A (GCG→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (148/121);  total (149/121)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 270 total aligned reads displayed.

GCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAC  >  CP009273/3111289‑3111579
                                                                                                                                                 |                                                                                                                                                 
          gTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACtt                                                                                                                                      >  1:3751586/1‑149 (MQ=255)
           ttGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtc                                                                                                                                     <  2:4181054/149‑1 (MQ=255)
             ggATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTtcat                                                                                                                                   <  2:1970957/149‑1 (MQ=255)
                 aaCGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCAcc                                                                                                                               <  2:4483175/149‑1 (MQ=255)
                   cGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCa                                                                                                                             <  2:2089987/149‑1 (MQ=255)
                    gagagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAg                                                                                                                            >  1:738755/1‑149 (MQ=255)
                    gagagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAg                                                                                                                            >  1:2156971/1‑149 (MQ=255)
                     agagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGt                                                                                                                           >  1:1398611/1‑149 (MQ=255)
                      gagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGtt                                                                                                                          >  2:3930544/1‑149 (MQ=255)
                      gagaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGtt                                                                                                                          >  2:2937910/1‑149 (MQ=255)
                       agaTTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtc                                                                                                                         >  1:1288494/1‑149 (MQ=255)
                          ttGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtcagt                                                                                                                      >  1:4015983/1‑149 (MQ=255)
                          ttGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtcagt                                                                                                                      >  2:2151162/1‑149 (MQ=255)
                          ttGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTtcagt                                                                                                                      >  1:2850843/1‑149 (MQ=255)
                               aaTCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAgcg                                                                                                                 >  1:3431114/1‑149 (MQ=255)
                                   tcGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAAt                                                                                                             <  1:804070/149‑1 (MQ=255)
                                   tcGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAAt                                                                                                             <  2:463636/149‑1 (MQ=255)
                                   tcGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAAt                                                                                                             >  1:3863285/1‑149 (MQ=255)
                                    cGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATg                                                                                                            <  1:2230630/149‑1 (MQ=255)
                                      ttGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGt                                                                                                          >  2:3029719/1‑149 (MQ=255)
                                              gTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTAt                                                                                                  >  2:3560328/1‑149 (MQ=255)
                                                 gggATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGc                                                                                               >  2:1476977/1‑149 (MQ=255)
                                                 gggATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGc                                                                                               >  2:2559732/1‑149 (MQ=255)
                                                   gATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcg                                                                                             <  1:2066327/149‑1 (MQ=255)
                                                    atatTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcgc                                                                                            <  2:4596259/149‑1 (MQ=255)
                                                     tatTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCcgcg                                                                                           >  1:3583517/1‑149 (MQ=255)
                                                          ggCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTg                                                                                      >  2:227388/1‑149 (MQ=255)
                                                          ggCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTg                                                                                      >  2:3489463/1‑149 (MQ=255)
                                                           gCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                     >  2:833277/1‑149 (MQ=255)
                                                           gCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                     >  1:2293282/1‑149 (MQ=255)
                                                           gCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGa                                                                                     >  2:3222065/1‑149 (MQ=255)
                                                             aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAc                                                                                   >  1:4527505/1‑149 (MQ=255)
                                                             aaaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAc                                                                                   >  1:643950/1‑149 (MQ=255)
                                                              aaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                  >  1:2365296/1‑149 (MQ=255)
                                                              aaGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAAcc                                                                                  >  1:1733290/1‑149 (MQ=255)
                                                                gACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCa                                                                                <  1:4707914/149‑1 (MQ=255)
                                                                 aCCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAg                                                                               <  2:1218400/149‑1 (MQ=255)
                                                                   cTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGtt                                                                             >  2:518719/1‑149 (MQ=255)
                                                                       cGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCaaa                                                                         <  1:1200826/149‑1 (MQ=255)
                                                                           cATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTa                                                                     <  1:735676/149‑1 (MQ=255)
                                                                            aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                    <  2:886123/149‑1 (MQ=255)
                                                                            aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                    <  2:569344/149‑1 (MQ=255)
                                                                            aTCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTAt                                                                    <  1:3318113/149‑1 (MQ=255)
                                                                               gTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGa                                                                 <  1:157820/149‑1 (MQ=255)
                                                                                tCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGAt                                                                <  2:4308412/149‑1 (MQ=255)
                                                                                   ggAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATAcc                                                             >  1:1515486/1‑149 (MQ=255)
                                                                                     aacaacACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaa                                                           >  1:1340219/1‑149 (MQ=255)
                                                                                     aacaacACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaa                                                           >  1:1341036/1‑149 (MQ=255)
                                                                                      acaacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaaa                                                          <  1:2270846/149‑1 (MQ=255)
                                                                                      acaacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCaaa                                                          <  2:1128815/149‑1 (MQ=255)
                                                                                        aacaCGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAgg                                                        >  2:1919466/1‑149 (MQ=255)
                                                                                         acacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGga                                                       >  1:4276892/1‑149 (MQ=255)
                                                                                         acacGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGga                                                       <  1:2601563/149‑1 (MQ=255)
                                                                                            cGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGAt                                                    <  1:1856468/149‑1 (MQ=255)
                                                                                             gggTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATg                                                   <  2:2189383/149‑1 (MQ=255)
                                                                                              ggTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATgg                                                  >  1:4351316/1‑149 (MQ=255)
                                                                                                tattatTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCTCCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGtt                                                <  1:421295/149‑1 (MQ=255)
                                                                                                 attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGttt                                               <  1:4090986/149‑1 (MQ=255)
                                                                                                 attattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGttt                                               <  2:1790017/149‑1 (MQ=255)
                                                                                                  ttattTTGCCCGGTCGTCGTATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                              <  1:1014902/149‑1 (MQ=255)
                                                                                                  ttattTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTc                                              <  1:3403361/149‑1 (MQ=255)
                                                                                                      tttGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGccc                                          >  2:2844576/1‑149 (MQ=255)
                                                                                                          cccGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGc                                      <  2:4685965/149‑1 (MQ=255)
                                                                                                          cccGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGc                                      <  2:3964609/149‑1 (MQ=255)
                                                                                                           ccGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCt                                     <  2:850550/149‑1 (MQ=255)
                                                                                                             ggTCGTCGAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTaa                                   <  1:3029893/149‑1 (MQ=255)
                                                                                                               tcgtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAg                                 <  2:1701810/149‑1 (MQ=255)
                                                                                                                 gtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGttt                                               <  2:4120034/133‑1 (MQ=255)
                                                                                                                 gtcgAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGa                               <  2:4394147/149‑1 (MQ=255)
                                                                                                                    gAATAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCGGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGaaaa                            >  1:4267744/1‑149 (MQ=255)
                                                                                                                     aaTAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAAt                           >  1:920853/1‑149 (MQ=255)
                                                                                                                       tAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGACAATTc                         >  2:3240645/1‑149 (MQ=255)
                                                                                                                       tAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTc                         >  2:718166/1‑149 (MQ=255)
                                                                                                                       tAGCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTc                         >  2:3435533/1‑149 (MQ=255)
                                                                                                                         gCGATGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAc                       >  2:2458559/1‑149 (MQ=255)
                                                                                                                          cGATGAATAAGCTGAACAATATTAGCCCCGCGAACTTCAACACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCAcc                      <  2:4251568/149‑1 (MQ=255)
                                                                                                                             tGAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAtt                   >  1:406656/1‑149 (MQ=255)
                                                                                                                              gAATAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCAtat                  >  2:3628617/1‑147 (MQ=255)
                                                                                                                                aTAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTc                <  2:3946086/149‑1 (MQ=255)
                                                                                                                                 tAAGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcc               <  1:4671977/149‑1 (MQ=255)
                                                                                                                                   aGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc             >  1:2897963/1‑149 (MQ=255)
                                                                                                                                   aGCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTcccc             >  2:1185001/1‑149 (MQ=255)
                                                                                                                                    gCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCt            >  2:2200874/1‑149 (MQ=255)
                                                                                                                                    gCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCt            <  2:1734185/149‑1 (MQ=255)
                                                                                                                                    gCTGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCt            <  2:4015983/149‑1 (MQ=255)
                                                                                                                                      tGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGt          <  2:971493/149‑1 (MQ=255)
                                                                                                                                      tGATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGt          >  1:197669/1‑149 (MQ=255)
                                                                                                                                       gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTc         <  1:3238532/149‑1 (MQ=255)
                                                                                                                                       gATCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTc         <  1:2247422/149‑1 (MQ=255)
                                                                                                                                        aTCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATg                                                   <  2:3789249/106‑1 (MQ=255)
                                                                                                                                        aTCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATg                                                   >  1:3789244/1‑106 (MQ=255)
                                                                                                                                         tCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCAc       >  2:2795155/1‑149 (MQ=255)
                                                                                                                                         tCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCAc       >  2:545066/1‑149 (MQ=255)
                                                                                                                                         tCAATATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCAc       >  1:3291858/1‑149 (MQ=255)
                                                                                                                                           aaTATTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGc     >  1:1680123/1‑149 (MQ=255)
                                                                                                                                            atatTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc    >  2:2690433/1‑149 (MQ=255)
                                                                                                                                            atatTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc    >  2:1349008/1‑149 (MQ=255)
                                                                                                                                            atatTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGcc    >  2:1349000/1‑149 (MQ=255)
                                                                                                                                             tatTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTCAAGGAAAATTCACAATTTTCCCCTGTCACGCCa   >  2:2980441/1‑149 (MQ=255)
                                                                                                                                              atTAGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAc  >  2:3644871/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCGTCGCACCGTTGGATAACGAGAGATTGATAATCTCGTTGATCACGTTGGGATATTCGGCAAAGACCTTGCGTACATCGTCCGGAACAACACGGGTATTATTTTGCCCGGTCGTCGAATAGCGATGAATAAGCTGATCAATATTCGCCCCGCGAACTTCATCACCCAGTTCAGTCAGCGCAATGGTCGACTTATTGCCGCGCACTGAACCCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTTTCCCCTGTCACGCCAC  >  CP009273/3111289‑3111579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑