BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,220,448	C→A	7.7%	R286S (CGC→AGC)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,220,448	0	C	A	7.7%	1059.4 / 37.8	289	R286S (CGC→AGC)	ygjI	putative transporter
Reads supporting (aligned to +/- strand): ref base C (132/134); new base A (13/10); total (145/144)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCG  >  CP009273/3220300‑3220596

cGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATc                                                                                                                                                      >  2:3293904/1‑149 (MQ=255)

 gggATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCg                                                                                                                                                     >  1:3777098/1‑149 (MQ=255)

  ggATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGc                                                                                                                                                    <  1:2155781/149‑1 (MQ=255)

  ggATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGc                                                                                                                                                    >  1:4696916/1‑149 (MQ=255)

    aTTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCtt                                                                                                                                                  <  2:1659110/149‑1 (MQ=255)

    aTTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCtt                                                                                                                                                  <  2:3765072/149‑1 (MQ=255)

     tttttATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCttt                                                                                                                                                 >  2:242988/1‑149 (MQ=255)

      ttttATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTg                                                                                                                                                <  2:311596/149‑1 (MQ=255)

      ttttATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTg                                                                                                                                                <  1:1370470/149‑1 (MQ=255)

       tttATCGGCGTACTTTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGt                                                                                                                                               <  1:1835716/149‑1 (MQ=255)

       tttATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGt                                                                                                                                               <  1:415910/149‑1 (MQ=255)

          aTCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTTGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCgg                                                                                                                                            >  2:1512842/1‑149 (MQ=255)

                gTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGgt                                                                                                                                      <  2:2441784/149‑1 (MQ=255)

                 tACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGgtg                                                                                                                                     >  1:773359/1‑149 (MQ=255)

                       aTTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTGTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCatt                                                                                                                               >  2:2633582/1‑149 (MQ=255)

                        ttCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTa                                                                                                                              <  2:4004762/149‑1 (MQ=255)

                        gtCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTa                                                                                                                              <  2:606459/148‑1 (MQ=255)

                         tCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTAc                                                                                                                             <  2:3466812/149‑1 (MQ=255)

                           cGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACcg                                                                                                                           <  2:4357418/149‑1 (MQ=255)

                            gTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACcgc                                                                                                                          >  2:1428179/1‑149 (MQ=255)

                             tctcCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACcgcg                                                                                                                         >  2:3020568/1‑149 (MQ=255)

                                cctcGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATg                                                                                                                      >  1:3522470/1‑149 (MQ=255)

                                 ctcGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGt                                                                                                                     >  2:3627575/1‑149 (MQ=255)

                                  tcGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGtt                                                                                                                    >  1:759265/1‑149 (MQ=255)

                                   cGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTc                                                                                                                   <  1:3450321/149‑1 (MQ=255)

                                     gTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCgg                                                                                                                 >  2:2214461/1‑149 (MQ=255)

                                      tGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGt                                                                                                                >  2:2373238/1‑149 (MQ=255)

                                       gCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGtt                                                                                                               >  2:1792538/1‑149 (MQ=255)

                                         tGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTcc                                                                                                             <  1:4090254/149‑1 (MQ=255)

                                         tGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTcc                                                                                                             <  1:2200532/149‑1 (MQ=255)

                                          gATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTccc                                                                                                            <  1:2729275/149‑1 (MQ=255)

                                               aCGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCctgctg                                                                                                       <  2:2408890/149‑1 (MQ=255)

                                                cGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGa                                                                                                      <  2:1228163/149‑1 (MQ=255)

                                                 gTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAt                                                                                                     <  1:433298/149‑1 (MQ=255)

                                                 gTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAt                                                                                                     >  1:1692086/1‑149 (MQ=255)

                                                  tCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtg                                                                                                    >  2:2103059/1‑149 (MQ=255)

                                                    ttCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtgtg                                                                                                  >  2:40462/1‑149 (MQ=255)

                                                    ttCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtgtg                                                                                                  >  1:693906/1‑149 (MQ=255)

                                                    ttCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtgtg                                                                                                  >  1:2555615/1‑149 (MQ=255)

                                                    ttCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGAtgtg                                                                                                  >  2:1411684/1‑149 (MQ=255)

                                                      cGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGa                                                                                                <  1:3827364/149‑1 (MQ=255)

                                                       gTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGAc                                                                                               <  2:4594055/149‑1 (MQ=255)

                                                          agcagcAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGc                                                                                            >  1:1623525/1‑149 (MQ=255)

                                                           gcagcaAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCGTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCa                                                                                           >  2:4630753/1‑149 (MQ=255)

                                                           gcagcaAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCa                                                                                           >  1:4527680/1‑149 (MQ=255)

                                                           gcagcaAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCa                                                                                           >  1:57086/1‑149 (MQ=255)

                                                               caAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTAATTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGc                                                                                       <  1:2270614/149‑1 (MQ=255)

                                                                 aaGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCg                                                                                     <  2:3805406/149‑1 (MQ=255)

                                                                   gagTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGt                                                                                   <  1:2323259/149‑1 (MQ=255)

                                                                    agTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTg                                                                                  <  1:1524157/149‑1 (MQ=255)

                                                                    agTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTg                                                                                  <  1:3355565/149‑1 (MQ=255)

                                                                    agTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTg                                                                                  <  1:2863184/149‑1 (MQ=255)

                                                                    agTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTg                                                                                  <  2:3217420/149‑1 (MQ=255)

                                                                            tttACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAAtttt                                                                          >  2:4223737/1‑149 (MQ=255)

                                                                              tACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTttct                                                                        >  1:4292717/1‑149 (MQ=255)

                                                                                ccGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTttcttc                                                                      <  2:851316/149‑1 (MQ=255)

                                                                                  ggcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTtctc                                                                    <  1:1495514/149‑1 (MQ=255)

                                                                                   gcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAACATTTTCTTCTcc                                                                   >  2:2962917/1‑149 (MQ=255)

                                                                                   gcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTcc                                                                   <  2:1135680/149‑1 (MQ=255)

                                                                                   gcggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTcc                                                                   <  1:3949586/149‑1 (MQ=255)

                                                                                     ggATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGa                                                                 >  2:264973/1‑149 (MQ=255)

                                                                                      gATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGaa                                                                >  1:2818368/1‑149 (MQ=255)

                                                                                       aTCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGaaa                                                               <  1:1845554/149‑1 (MQ=255)

                                                                                         cGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATc                                                             <  1:1808721/149‑1 (MQ=255)

                                                                                           gTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATccc                                                           >  1:4395816/1‑149 (MQ=255)

                                                                                           gTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATccc                                                           <  2:154313/149‑1 (MQ=255)

                                                                                           gTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATccc                                                           >  2:4780830/1‑149 (MQ=255)

                                                                                             gCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCgg                                                         <  1:1128044/149‑1 (MQ=255)

                                                                                              cAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGa                                                        <  1:3757852/149‑1 (MQ=255)

                                                                                               aGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGaa                                                       <  2:2005109/149‑1 (MQ=255)

                                                                                                  tATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGc                                                    <  1:1475126/149‑1 (MQ=255)

                                                                                                      ccACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCt                                                >  1:1083907/1‑149 (MQ=255)

                                                                                                      ccACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCt                                                >  1:3245901/1‑149 (MQ=255)

                                                                                                       cACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCtt                                               >  1:2605152/1‑149 (MQ=255)

                                                                                                         cGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTg                                             <  2:2554488/149‑1 (MQ=255)

                                                                                                          ggCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTgg                                            <  1:93692/149‑1 (MQ=255)

                                                                                                           gCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGt                                           >  1:1907618/1‑149 (MQ=255)

                                                                                                           gCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGt                                           <  2:689573/149‑1 (MQ=255)

                                                                                                             aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTaa                                         <  1:4236423/149‑1 (MQ=255)

                                                                                                             aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTaa                                         <  1:195463/149‑1 (MQ=255)

                                                                                                             aTGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTaa                                         <  1:867684/149‑1 (MQ=255)

                                                                                                               ggcggcGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGa                                       >  2:2357411/1‑149 (MQ=255)

                                                                                                                       aTTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTc                               <  1:1418185/149‑1 (MQ=255)

                                                                                                                            ggTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACt                          >  1:3578698/1‑149 (MQ=255)

                                                                                                                                tACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAAc                      >  2:1854888/1‑149 (MQ=255)

                                                                                                                                 aCCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACg                     >  2:3702843/1‑149 (MQ=255)

                                                                                                                                 aCCGGAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACg                     >  2:1303282/1‑149 (MQ=255)

                                                                                                                                   cGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGaa                   >  2:1012325/1‑149 (MQ=255)

                                                                                                                                     gAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGaaaa                 >  1:3109587/1‑149 (MQ=255)

                                                                                                                                     gAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGaaaa                 <  2:1602676/149‑1 (MQ=255)

                                                                                                                                     gAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTAACGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAATAAAACCGTCGAACTGAACGaaaa                 >  1:1226334/1‑149 (MQ=255)

                                                                                                                                     gAAGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGaaaa                 >  1:232813/1‑149 (MQ=255)

                                                                                                                                      aaGCGTTGATGAATAGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGACCTGAACGAAAAc                >  1:3884944/1‑149 (MQ=255)

                                                                                                                                         cGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGc             <  2:397394/149‑1 (MQ=255)

                                                                                                                                          gTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCg            >  2:565442/1‑149 (MQ=255)

                                                                                                                                           ttGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGt           <  2:1447721/149‑1 (MQ=255)

                                                                                                                                             gatgaATCGCTTTGTCGGTCTGGTGTCCTTTACCGGGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTc         <  1:1709554/149‑1 (MQ=255)

                                                                                                                                             gatgaATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTc         <  2:3728392/149‑1 (MQ=255)

                                                                                                                                                 aaTCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgc     >  1:2991713/1‑149 (MQ=255)

                                                                                                                                                 aaTCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGgc     <  1:2289653/149‑1 (MQ=255)

CGGGATTTTTATCGGCGTACTGTATTCCGTCTCCTCGGTGCTGATTAACGTCTTCGTCAGCAGCAAAGAGTTGAAATTTACCGGCGGATCGGTGCAGGTATTCCACGGCATGGCGGCGTATTTTGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCG  >  CP009273/3220300‑3220596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`