Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 255,487 A→T 85.4% S109T (TCT→ACT)  phoE ← outer membrane phosphoporin protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273255,4870AT85.4% 734.7 / 106.0 268S109T (TCT→ACT) phoEouter membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base A (19/20);  new base T (110/119);  total (129/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

Only 100 of 271 total aligned reads displayed.

CGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCA  >  CP009273/255340‑255635
                                                                                                                                                   |                                                                                                                                                    
 ggTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAc                                                                                                                                                    <  1:3634357/149‑1 (MQ=255)
  gtgtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAcc                                                                                                                                                   >  2:3357644/1‑149 (MQ=255)
   tgtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAccc                                                                                                                                                  >  1:2247512/1‑149 (MQ=255)
        cGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATc                                                                                                                                             >  1:2986436/1‑149 (MQ=255)
          aTACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCtt                                                                                                                                           <  2:1245896/149‑1 (MQ=255)
           tACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                          <  2:3229442/149‑1 (MQ=255)
           tACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                          <  1:2092131/149‑1 (MQ=255)
            aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTta                                                                                                                                         <  2:2864683/149‑1 (MQ=255)
            aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTta                                                                                                                                         <  2:2295597/149‑1 (MQ=255)
            aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTta                                                                                                                                         <  1:2280147/149‑1 (MQ=255)
             cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtat                                                                                                                                        <  2:3681172/149‑1 (MQ=255)
             cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtat                                                                                                                                        <  1:3034625/149‑1 (MQ=255)
             cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtat                                                                                                                                        <  1:1286941/149‑1 (MQ=255)
                  ccAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                   >  2:3796064/1‑149 (MQ=255)
                      aCCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAcc                                                                                                                               <  2:4388447/149‑1 (MQ=255)
                      aCCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAcc                                                                                                                               <  2:1637765/149‑1 (MQ=255)
                       ccGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAccc                                                                                                                              <  1:327057/149‑1 (MQ=255)
                               gcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCCAAAg                                                                                                                      >  2:4750438/1‑149 (MQ=255)
                               gcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAg                                                                                                                      >  1:4128682/1‑149 (MQ=255)
                                  tttGGTCATATAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCga                                                                                                                   <  2:2563035/149‑1 (MQ=255)
                                  tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCga                                                                                                                   >  2:4415580/1‑149 (MQ=255)
                                      gTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAcg                                                                                                               >  1:715390/1‑149 (MQ=255)
                                       tCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAcgc                                                                                                              <  2:4132736/149‑1 (MQ=255)
                                          tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtt                                                                                                           >  1:1059306/1‑149 (MQ=255)
                                          tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtt                                                                                                           >  1:2620810/1‑149 (MQ=255)
                                           aaaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttt                                                                                                          <  2:647196/149‑1 (MQ=255)
                                           aaaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttt                                                                                                          <  2:4559627/149‑1 (MQ=255)
                                                 gTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                    >  2:1989237/1‑149 (MQ=255)
                                                 gTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                    >  1:998004/1‑149 (MQ=255)
                                                  tctgTCTGCGCCGAGGAATCGCCACCAAATTCCGTGAACATATCGGTCCAGGCTTCCACGTCATACAACGCACCCAGGTTACGAACATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCtg                                                                                                   <  1:2441802/146‑1 (MQ=255)
                                                     gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGc                                                                                                >  2:2474215/1‑149 (MQ=255)
                                                     gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGc                                                                                                <  2:1915894/149‑1 (MQ=255)
                                                     gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGc                                                                                                <  2:2706569/149‑1 (MQ=255)
                                                     gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGc                                                                                                <  2:4306763/149‑1 (MQ=255)
                                                     gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCATTTTTTGCTGTGc                                                                                                >  2:2966419/1‑149 (MQ=255)
                                                       cTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAg                                                                                              <  1:93609/149‑1 (MQ=255)
                                                         gcgcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTa                                                                                            <  1:1655413/149‑1 (MQ=255)
                                                              gAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCAct                                                                                       <  1:4466336/149‑1 (MQ=255)
                                                               aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                      >  1:1730383/1‑149 (MQ=255)
                                                                 gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActctc                                                                                    <  1:555465/149‑1 (MQ=255)
                                                                 gAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActctc                                                                                    >  1:1324336/1‑149 (MQ=255)
                                                                       ccaccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCttt                                                                              >  1:2284534/1‑149 (MQ=255)
                                                                       ccaccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCttt                                                                              <  2:87487/149‑1 (MQ=255)
                                                                        caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTtta                                                                             >  1:1570599/1‑149 (MQ=255)
                                                                        caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTtta                                                                             >  1:3502176/1‑149 (MQ=255)
                                                                        caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTtta                                                                             <  2:831515/149‑1 (MQ=255)
                                                                         accaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttat                                                                            >  2:3552674/1‑149 (MQ=255)
                                                                           caAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttatta                                                                          <  1:3025796/149‑1 (MQ=255)
                                                                             aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAcc                                                                        >  2:4209683/1‑149 (MQ=255)
                                                                             aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAcc                                                                        <  1:1000304/149‑1 (MQ=255)
                                                                              aTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCg                                                                       <  2:1844361/149‑1 (MQ=255)
                                                                                 ccGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCa                                                                    <  1:1590932/149‑1 (MQ=255)
                                                                                   gggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCaaa                                                                  >  2:3071102/1‑149 (MQ=255)
                                                                                   gggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCaaa                                                                  >  2:2451407/1‑149 (MQ=255)
                                                                                   gggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCaaa                                                                  >  1:1937625/1‑149 (MQ=255)
                                                                                       aCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActct                                                              <  1:2024555/149‑1 (MQ=255)
                                                                                         atatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGc                                                            >  2:2042735/1‑149 (MQ=255)
                                                                                          tatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCt                                                           <  1:3463630/149‑1 (MQ=255)
                                                                                           atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCtt                                                          >  2:1267848/1‑149 (MQ=255)
                                                                                           atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCtt                                                          >  2:3022654/1‑149 (MQ=255)
                                                                                            tCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTc                                                         >  2:885196/1‑149 (MQ=255)
                                                                                              ggTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTccc                                                       <  1:2137760/149‑1 (MQ=255)
                                                                                              ggTCCAGCCTACCCCCTCAAACACCGCCCCCATGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCCAACTCTGCGTccc                                                       <  1:3199866/149‑1 (MQ=255)
                                                                                                  cAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACg                                                   >  2:3604865/1‑149 (MQ=255)
                                                                                                   aGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGa                                                  >  1:793816/1‑149 (MQ=255)
                                                                                                   aGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGa                                                  >  2:536821/1‑149 (MQ=255)
                                                                                                     gCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAcc                                                >  2:4057825/1‑149 (MQ=255)
                                                                                                      cTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCa                                               >  1:2763054/1‑149 (MQ=255)
                                                                                                      cTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCa                                               >  1:3348341/1‑149 (MQ=255)
                                                                                                        tCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATa                                             >  1:490224/1‑149 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCACACTCTGCTTCCCAACGACCATAAc                                           >  2:3166961/1‑149 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAc                                           >  1:4259383/1‑149 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAc                                           <  2:1616877/149‑1 (MQ=255)
                                                                                                             gTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcag                                        >  2:3283454/1‑149 (MQ=255)
                                                                                                                        cccccAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCg                             <  2:2327825/149‑1 (MQ=255)
                                                                                                                        cccccAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCg                             >  2:199117/1‑149 (MQ=255)
                                                                                                                          cccAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGtt                           <  2:2763369/149‑1 (MQ=255)
                                                                                                                           ccAGGTTACGACCATAATCGAAAGTACCCCAAGCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCGTTCTTACCGGCAAACTCTGCTTCCCAACGACCCTAACCAGTCAGTTGAGCGGTa                          >  2:753339/1‑149 (MQ=255)
                                                                                                                            cAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTaa                         <  1:3796048/149‑1 (MQ=255)
                                                                                                                            cAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTaa                         <  1:2120130/149‑1 (MQ=255)
                                                                                                                             aGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAt                        >  2:1329131/1‑149 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCACAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATAACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGATAAtat                      >  2:3994850/1‑147 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                      >  1:4577044/1‑149 (MQ=255)
                                                                                                                                ttACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtg                     >  1:2186379/1‑149 (MQ=255)
                                                                                                                                   cGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTgtgt                  >  1:4028146/1‑149 (MQ=255)
                                                                                                                                    gACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGtt                 <  2:57340/149‑1 (MQ=255)
                                                                                                                                     aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt                <  1:2673801/149‑1 (MQ=255)
                                                                                                                                     aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt                >  1:1289494/1‑149 (MQ=255)
                                                                                                                                     aCCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt                >  2:3361235/1‑149 (MQ=255)
                                                                                                                                     aCCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt                <  1:11881/149‑1 (MQ=255)
                                                                                                                                        aTAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGc             <  2:2735562/149‑1 (MQ=255)
                                                                                                                                         tAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGcc            >  1:917695/1‑149 (MQ=255)
                                                                                                                                         tAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGcc            <  1:4368358/149‑1 (MQ=255)
                                                                                                                                          aaTCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGATGTGCAGTATCACTCACTGCTTTATTACCGGCAAACTCTGATTCCCAACGACCATAAAAAGACAGTTGATCGTTAATTTGTGTTTCGCCt           >  1:4047874/1‑149 (MQ=255)
                                                                                                                                           aTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCtt          <  2:3760484/149‑1 (MQ=255)
                                                                                                                                           aTCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCtt          <  1:2526114/149‑1 (MQ=255)
                                                                                                                                            tCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCttt         <  1:2297164/149‑1 (MQ=255)
                                                                                                                                                aaGTACCCAAATCTTTATATTTCACCCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaaa     <  1:2415455/149‑1 (MQ=255)
                                                                                                                                                aaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaaa     <  1:344074/149‑1 (MQ=255)
                                                                                                                                                   aaCCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCa  >  1:483715/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCA  >  CP009273/255340‑255635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑