BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,239,833	G→T	9.5%	A457A (GCG→GCT)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,239,833	0	G	T	9.5%	1027.1 / 52.4	283	A457A (GCG→GCT)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base G (104/152); new base T (11/16); total (115/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 286 total aligned reads displayed.

TGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAT  >  CP009273/3239685‑3239980

tGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCg                                                                                                                                                     >  2:2988307/1‑149 (MQ=255)

tGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCg                                                                                                                                                     >  2:3411040/1‑149 (MQ=255)

  tCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGAt                                                                                                                                                   <  2:2277462/149‑1 (MQ=255)

  tCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGAt                                                                                                                                                   <  1:3063729/149‑1 (MQ=255)

    cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTg                                                                                                                                                 <  1:4565072/149‑1 (MQ=255)

    cGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTg                                                                                                                                                 >  2:800607/1‑149 (MQ=255)

      cATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAg                                                                                                                                               >  1:4209111/1‑149 (MQ=255)

       aTGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAgg                                                                                                                                              <  1:2759378/149‑1 (MQ=255)

        tggctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGt                                                                                                                                             >  2:4633832/1‑149 (MQ=255)

              gcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCa                                                                                                                                       <  1:4564330/149‑1 (MQ=255)

               cAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAg                                                                                                                                      <  2:727583/149‑1 (MQ=255)

                aaGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAgg                                                                                                                                     <  1:2257508/149‑1 (MQ=255)

                 aGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGa                                                                                                                                    <  1:3646294/149‑1 (MQ=255)

                 aGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGa                                                                                                                                    >  1:4279458/1‑149 (MQ=255)

                  gTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGaa                                                                                                                                   <  1:1093553/149‑1 (MQ=255)

                   tACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGaaa                                                                                                                                  <  1:133681/149‑1 (MQ=255)

                     cGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAaca                                                                                                                                <  1:749975/149‑1 (MQ=255)

                       cTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAAcaca                                                                                                                              >  1:1651793/1‑149 (MQ=255)

                       cTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAAcaca                                                                                                                              >  2:1292450/1‑149 (MQ=255)

                       cTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAAcaca                                                                                                                              <  1:440973/149‑1 (MQ=255)

                         gTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATa                                                                                                                            >  1:391518/1‑149 (MQ=255)

                          tttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATaa                                                                                                                           <  1:3827740/149‑1 (MQ=255)

                          ttggcgcTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATaa                                                                                                                           <  2:200442/146‑1 (MQ=255)

                           ttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAAc                                                                                                                          <  1:347227/149‑1 (MQ=255)

                           ttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAAc                                                                                                                          <  2:3463263/149‑1 (MQ=255)

                           ttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAAc                                                                                                                          <  1:65720/149‑1 (MQ=255)

                             gcgcTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGa                                                                                                                        >  2:1738583/1‑149 (MQ=255)

                              cgcTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCTATTGAGGTGGCGCAGGAAACACATAACGAt                                                                                                                       <  2:4080756/149‑1 (MQ=255)

                               gcTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATc                                                                                                                      <  1:699540/149‑1 (MQ=255)

                                   gTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCccgc                                                                                                                  >  1:2820587/1‑149 (MQ=255)

                                    ttGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCccgca                                                                                                                 >  2:216070/1‑149 (MQ=255)

                                       tCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCccgcaccg                                                                                                              >  2:2235523/1‑149 (MQ=255)

                                          gTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCaa                                                                                                           <  2:862916/149‑1 (MQ=255)

                                            gCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGACACACATAACGATCCCGCACCGCAACa                                                                                                         >  2:4221853/1‑149 (MQ=255)

                                               cTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTa                                                                                                      >  1:4347973/1‑149 (MQ=255)

                                                  gCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACg                                                                                                   <  1:377319/149‑1 (MQ=255)

                                                     gACACCATCGGCTTCAGCCCGCTGTTCGCAGGTCTGGCAGTGTTCTACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGTGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGttt                                                                                                <  1:1707500/149‑1 (MQ=255)

                                                        acCATCTGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCgt                                                                                             >  2:4504996/1‑149 (MQ=255)

                                                        acCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCgt                                                                                             <  2:2560160/149‑1 (MQ=255)

                                                        acCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCgt                                                                                             <  2:167945/149‑1 (MQ=255)

                                                           aTCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTa                                                                                          <  1:4226710/149‑1 (MQ=255)

                                                            tCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTaa                                                                                         <  1:1593197/149‑1 (MQ=255)

                                                             cGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCTATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAg                                                                                        <  1:468368/149‑1 (MQ=255)

                                                             cGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAg                                                                                        <  1:2581266/149‑1 (MQ=255)

                                                             cGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAg                                                                                        <  2:3911901/149‑1 (MQ=255)

                                                              ggCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGt                                                                                       >  1:1166624/1‑149 (MQ=255)

                                                              ggCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGt                                                                                       >  1:2334419/1‑149 (MQ=255)

                                                               gCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGtt                                                                                      <  2:3363856/149‑1 (MQ=255)

                                                                 ttCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGtttt                                                                                    >  2:1106179/1‑149 (MQ=255)

                                                                 ttCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGtttt                                                                                    <  1:92448/149‑1 (MQ=255)

                                                                    aGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGt                                                                                 <  2:2431020/149‑1 (MQ=255)

                                                                      cccGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGttt                                                                               <  1:1513062/149‑1 (MQ=255)

                                                                       ccGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTc                                                                              >  2:1938032/1‑149 (MQ=255)

                                                                       ccGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTc                                                                              >  1:189817/1‑149 (MQ=255)

                                                                       ccGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTc                                                                              <  1:3391168/149‑1 (MQ=255)

                                                                         gCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTccc                                                                            >  1:1840306/1‑149 (MQ=255)

                                                                         gCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTccc                                                                            <  2:4140669/149‑1 (MQ=255)

                                                                          cTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCAGGTTATCTGGACCGTGTTGCAGAACAAACCGGCTATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCt                                                                           <  1:1329311/149‑1 (MQ=255)

                                                                             ttCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCa                                                                        >  1:3979362/1‑149 (MQ=255)

                                                                               cGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCaaa                                                                      <  1:4745619/149‑1 (MQ=255)

                                                                                gCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAg                                                                     <  1:2976697/149‑1 (MQ=255)

                                                                                  aGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAgcc                                                                   <  1:1429377/149‑1 (MQ=255)

                                                                                      cTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCt                                                               <  1:577164/149‑1 (MQ=255)

                                                                                         gCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtct                                                            <  1:1054949/149‑1 (MQ=255)

                                                                                         gCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtct                                                            <  2:1548926/149‑1 (MQ=255)

                                                                                         gCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtct                                                            >  1:466117/1‑149 (MQ=255)

                                                                                           aGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCa                                                          <  2:184380/149‑1 (MQ=255)

                                                                                            gtgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAg                                                         >  1:1049512/1‑149 (MQ=255)

                                                                                            gtgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAg                                                         <  2:3873281/149‑1 (MQ=255)

                                                                                             tgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAgg                                                        <  1:1238761/149‑1 (MQ=255)

                                                                                                  gACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCt                                                   <  2:2777872/149‑1 (MQ=255)

                                                                                                   aCCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCtt                                                  <  1:867373/149‑1 (MQ=255)

                                                                                                     cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCtttt                                                >  1:2434831/1‑149 (MQ=255)

                                                                                                       gTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtc                                              <  2:3309443/149‑1 (MQ=255)

                                                                                                        ttAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtca                                             >  1:995514/1‑149 (MQ=255)

                                                                                                         tAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcat                                            <  1:3780628/149‑1 (MQ=255)

                                                                                                          aGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatc                                           <  1:2138990/149‑1 (MQ=255)

                                                                                                          aGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatc                                           >  2:4672374/1‑149 (MQ=255)

                                                                                                           ggTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatca                                          <  1:2749884/149‑1 (MQ=255)

                                                                                                            gTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCAc                                         <  1:1877488/149‑1 (MQ=255)

                                                                                                            gTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCAc                                         <  2:483976/149‑1 (MQ=255)

                                                                                                            gTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCAc                                         <  1:510895/149‑1 (MQ=255)

                                                                                                                  tGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAg                                   >  2:525183/1‑149 (MQ=255)

                                                                                                                  tGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAg                                   >  2:3190839/1‑149 (MQ=255)

                                                                                                                   ggTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCCCCGCAACATTAACGTTGCGTGTAAGTTTTAGGTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGt                                  <  2:3090271/149‑1 (MQ=255)

                                                                                                                    gTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTa                                 >  1:265120/1‑149 (MQ=255)

                                                                                                                    gTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTa                                 >  1:2173288/1‑149 (MQ=255)

                                                                                                                    gTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGAAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTa                                 <  2:3711865/149‑1 (MQ=255)

                                                                                                                        tCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGc                             <  1:3497361/149‑1 (MQ=255)

                                                                                                                        tCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGGGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTCGAGc                             >  2:4361202/1‑149 (MQ=255)

                                                                                                                         cTGGACCGTGTTGCAGAACAAACCGGCTATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCt                            <  1:3194267/149‑1 (MQ=255)

                                                                                                                                  gtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCa                   >  1:2399309/1‑149 (MQ=255)

                                                                                                                                     gCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCaaaa                >  1:3563640/1‑149 (MQ=255)

                                                                                                                                        gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTg             >  1:2047161/1‑149 (MQ=255)

                                                                                                                                        gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTg             >  2:843367/1‑149 (MQ=255)

                                                                                                                                          aCAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGt           <  2:1693937/149‑1 (MQ=255)

                                                                                                                                           cAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGta          <  1:3280036/149‑1 (MQ=255)

                                                                                                                                             aaCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGtata        >  2:2854367/1‑149 (MQ=255)

                                                                                                                                              aCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATaa       <  2:1359099/149‑1 (MQ=255)

                                                                                                                                                cGGCTATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTATAGCTAAACTAGCAAAAGTGGTATAACa     >  1:969851/1‑149 (MQ=255)

TGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAT  >  CP009273/3239685‑3239980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`