Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,248,241 G→A 5.5% G188G (GGG→GGA)  yhaK → redox‑sensitive bicupin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,248,2410GA 5.5% 1014.6 / 30.5 292G188G (GGG→GGA) yhaKredox‑sensitive bicupin
Reads supporting (aligned to +/- strand):  ref base G (141/127);  new base A (9/7);  total (150/142)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 299 total aligned reads displayed.

AGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTAC  >  CP009273/3248096‑3248389
                                                                                                                                                 |                                                                                                                                                    
agcaATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGaaa                                                                                                                                                   <  1:358766/149‑1 (MQ=255)
 gcaATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAAt                                                                                                                                                  <  2:56006/149‑1 (MQ=255)
    aTTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTc                                                                                                                                               >  2:156621/1‑149 (MQ=255)
     ttAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCa                                                                                                                                              <  1:1277889/149‑1 (MQ=255)
      tAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCAt                                                                                                                                             >  2:2458837/1‑149 (MQ=255)
        aTCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGTAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATgc                                                                                                                                           >  2:3719671/1‑149 (MQ=255)
              tcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTAc                                                                                                                                     <  1:3790580/149‑1 (MQ=255)
               cgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTCTTTGCAATCGATTCACGGGAAATTTCATGCGCTTACg                                                                                                                                    >  1:4447306/1‑149 (MQ=255)
                gccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGc                                                                                                                                   <  2:801228/149‑1 (MQ=255)
                 ccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGca                                                                                                                                  >  2:250682/1‑149 (MQ=255)
                 ccAGAGGGTGCGATGGGAAGCCTGCAAGTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGca                                                                                                                                  <  2:3775188/149‑1 (MQ=255)
                 caagagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGca                                                                                                                                  >  1:1487261/3‑149 (MQ=255)
                   agagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGcatc                                                                                                                                <  1:3398323/149‑1 (MQ=255)
                   agagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGcatc                                                                                                                                >  2:2607907/1‑149 (MQ=255)
                    gagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGcatca                                                                                                                               <  1:3339008/149‑1 (MQ=255)
                    gagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCAGGGGCCCCGCGCGTATTTGCAATCGATTCACGGGCAATTTCATGCGCTTACGcatca                                                                                                                               >  1:2531278/1‑149 (MQ=255)
                      gggTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATg                                                                                                                             <  1:4193892/149‑1 (MQ=255)
                             aTGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGaa                                                                                                                      <  1:3627234/149‑1 (MQ=255)
                             aTGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGaa                                                                                                                      <  1:662916/149‑1 (MQ=255)
                             aTGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGaa                                                                                                                      >  2:3563800/1‑149 (MQ=255)
                              tGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGaaa                                                                                                                     >  1:4731819/1‑149 (MQ=255)
                               gggAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCCTATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAg                                                                                                                    >  2:4066846/1‑149 (MQ=255)
                               gggAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAg                                                                                                                    <  2:4745816/149‑1 (MQ=255)
                                ggAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAgc                                                                                                                   >  2:2314319/1‑149 (MQ=255)
                                   aGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAgcggc                                                                                                                <  1:2074528/149‑1 (MQ=255)
                                    gCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAgcggcg                                                                                                               >  1:215422/1‑149 (MQ=255)
                                     ccTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGgcgc                                                                                                              >  2:4737312/1‑149 (MQ=255)
                                     ccTGCAATTACGCCAGCAAGTGTGGCTGAACAATTACGTGCACGACATAGGCTAAAGTGAGTATTTCCTGTTGCATGGGCCACGGGTGTATTTGCAATCGATTCACGTGAAATTTCTTGCGCTTACGCATAATGAAGAGAAAGCGgcgc                                                                                                              <  2:840545/149‑1 (MQ=255)
                                      ccgCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCt                                                                                                             <  2:1372488/147‑1 (MQ=255)
                                        gCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGTAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGa                                                                                                           <  2:2939898/149‑1 (MQ=255)
                                           aTTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCt                                                                                                        <  2:3120481/149‑1 (MQ=255)
                                           aTTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCt                                                                                                        >  1:2140712/1‑149 (MQ=255)
                                             tACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGc                                                                                                      >  1:4344892/1‑149 (MQ=255)
                                                gCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGt                                                                                                   >  2:4598909/1‑149 (MQ=255)
                                                gCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGt                                                                                                   >  2:1460562/1‑149 (MQ=255)
                                                gCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGt                                                                                                   <  2:3955607/149‑1 (MQ=255)
                                                gCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCAGGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGt                                                                                                   <  2:3438736/149‑1 (MQ=255)
                                                 ccAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTg                                                                                                  <  2:3490133/149‑1 (MQ=255)
                                                  cAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCTAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGa                                                                                                 <  2:1317804/149‑1 (MQ=255)
                                                   aGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGAt                                                                                                >  1:4074229/1‑149 (MQ=255)
                                                      aaGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATggg                                                                                             >  1:1056722/1‑149 (MQ=255)
                                                        gtttgtcTGCACAATATGGTTCTATAAAATTGCGAACGTGCGAATTTCCTTTTGATTGGGCCTGGCGCTTTTTTGAATTCGATTCAGGGTAAATTTCAGGCGCTTACGATTCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGc                                                                                           <  1:4613824/143‑1 (MQ=255)
                                                        gtgtGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGc                                                                                           >  2:104579/1‑149 (MQ=255)
                                                           tgGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGtt                                                                                        <  2:3812309/149‑1 (MQ=255)
                                                               tGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTAtt                                                                                    >  2:140517/1‑149 (MQ=255)
                                                                  aCCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTCCGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGt                                                                                 <  2:3213835/149‑1 (MQ=255)
                                                                    aatatCGTGCTCGACAAAGGCTAAAGTGCTAATTTCCTTTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGTAAATTTCATGCGATTACGAATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGa                                                                               <  1:3354397/148‑1 (MQ=255)
                                                                     atatCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGAc                                                                              >  2:1825297/1‑149 (MQ=255)
                                                                        tCGTGCTCGACAAAGGCGAAAGTGCGAATGTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCAGGCGCGTACGCATCCTGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAg                                                                           <  2:4458322/149‑1 (MQ=255)
                                                                          gTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGc                                                                         >  1:4452508/1‑149 (MQ=255)
                                                                           tGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCt                                                                        <  2:3327817/149‑1 (MQ=255)
                                                                                 aCAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACAtt                                                                  >  2:2014631/1‑149 (MQ=255)
                                                                                  cAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTGTATTCGTGACGAGGCTCACATTa                                                                 >  2:3637296/1‑149 (MQ=255)
                                                                                   aaaGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTAc                                                                >  1:313903/1‑149 (MQ=255)
                                                                                    aaGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACg                                                               >  1:2575412/1‑149 (MQ=255)
                                                                                    aaGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACg                                                               >  2:2083388/1‑149 (MQ=255)
                                                                                     aGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGc                                                              >  2:1534780/1‑149 (MQ=255)
                                                                                      ggCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCt                                                             >  1:565891/1‑149 (MQ=255)
                                                                                          aaaGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGtt                                                         >  2:4533313/1‑149 (MQ=255)
                                                                                           aaGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTg                                                        >  1:1929094/1‑149 (MQ=255)
                                                                                              tGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCg                                                     >  1:3030890/1‑149 (MQ=255)
                                                                                                cGAATTTCCAGTTGCTTGGGCCACGCGGGTTTTTGCAATCGATTCACGGGAATTTTCATGCGCTTACGCATCATGAAGAGAACGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGAt                                                   <  1:1249977/149‑1 (MQ=255)
                                                                                                  aaTTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTc                                                 >  2:466926/1‑149 (MQ=255)
                                                                                                    tttCCAGTTGCATGGGCCACGCGCGTTTTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGTGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTccc                                               <  2:428604/149‑1 (MQ=255)
                                                                                                    tttCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTccc                                               >  2:2435907/1‑149 (MQ=255)
                                                                                                      tCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCa                                             >  1:1345498/1‑149 (MQ=255)
                                                                                                       ccAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCAc                                            >  1:2679659/1‑149 (MQ=255)
                                                                                                          gtggctggggCCTCGCGCTTATTTGGATTCGATTGACGGGAAATTTCAGGCGCTTAGGCATCTTGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCCCTgc                                         <  1:2611202/142‑1 (MQ=255)
                                                                                                              cATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTgcgcgc                                     <  1:2375667/149‑1 (MQ=255)
                                                                                                               aTGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCt                                    >  1:2829456/1‑149 (MQ=255)
                                                                                                                tGGGCCACGCGCGTTTTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAACGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCtt                                   <  1:1409195/149‑1 (MQ=255)
                                                                                                                 gggCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCttt                                  >  2:2263884/1‑149 (MQ=255)
                                                                                                                  ggCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTg                                 <  2:582187/149‑1 (MQ=255)
                                                                                                                   gCCACGCGCGTTTTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGc                                <  2:1487261/149‑1 (MQ=255)
                                                                                                                    ccccgcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTGTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCt                               >  1:4571978/4‑149 (MQ=255)
                                                                                                                    ccACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCt                               <  1:3284921/149‑1 (MQ=255)
                                                                                                                       cgcgcgTTTTTGAAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAACGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGAt                            <  1:4038147/149‑1 (MQ=255)
                                                                                                                       cgcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGAt                            <  1:4101634/149‑1 (MQ=255)
                                                                                                                        gcgggTATTTGCAATCGATTCACGGGAATTTTCATGCGATTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATa                           <  1:877092/149‑1 (MQ=255)
                                                                                                                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATa                           >  1:3720341/1‑149 (MQ=255)
                                                                                                                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATa                           <  2:1573146/149‑1 (MQ=255)
                                                                                                                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATa                           >  2:2782688/1‑149 (MQ=255)
                                                                                                                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATa                           >  1:4642857/1‑149 (MQ=255)
                                                                                                                        gcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTGACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGAtc                           <  2:3839370/149‑2 (MQ=255)
                                                                                                                            gTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGAtt                       <  2:2683733/149‑1 (MQ=255)
                                                                                                                            gTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGAtt                       <  2:1776505/149‑1 (MQ=255)
                                                                                                                            atATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGAtt                       <  2:1661564/148‑1 (MQ=255)
                                                                                                                              aTTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTg                     >  2:3610250/1‑149 (MQ=255)
                                                                                                                               tttGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGc                    >  2:869489/1‑149 (MQ=255)
                                                                                                                               tttGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGc                    >  1:590730/1‑149 (MQ=255)
                                                                                                                                  gCAATCGATTCACGTGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGAAGAGGCTAACATTAAGCTGGTTGCCGATTCCCCACTGAGCGCTTTGCTGATATATTTTCCTg                 >  1:2015707/1‑149 (MQ=255)
                                                                                                                                  gCAATCGAGTCACGGGAAATTTCAGGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTg                 <  1:3237073/149‑1 (MQ=255)
                                                                                                                                    aaTCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTc               <  1:4272413/149‑1 (MQ=255)
                                                                                                                                     aTCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCt              >  2:3292453/1‑149 (MQ=255)
                                                                                                                                      tCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTa             >  2:3994738/1‑149 (MQ=255)
                                                                                                                                       cGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAg            <  2:733132/149‑1 (MQ=255)
                                                                                                                                        gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgt           >  1:3485694/1‑149 (MQ=255)
                                                                                                                                          ttCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgttg         >  1:1982164/1‑149 (MQ=255)
                                                                                                                                             aCGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGttt      >  2:4338307/1‑149 (MQ=255)
                                                                                                                                               gggAAAGTTCCGGCGCTTACGCATCATGACGAGAACGCGGCGCTGACCTGCGGTGAGGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCGTTGCTGATAGATTTGCCTGTCTAGTTGttttt    <  1:3387963/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTAC  >  CP009273/3248096‑3248389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑