Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 297,724 A→C 10.4% D187E (GAT→GAG)  paoA ← PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273297,7240AC10.4% 1071.6 / 21.7 371D187E (GAT→GAG) paoAPaoABC aldehyde oxidoreductase, 2Fe‑2S subunit
Reads supporting (aligned to +/- strand):  ref base A (213/109);  new base C (2/47);  total (215/156)
Fisher's exact test for biased strand distribution p-value = 9.95e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

CGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACC  >  CP009273/297576‑297873
                                                                                                                                                    |                                                                                                                                                     
cGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAAt                                                                                                                                                      <  1:2873102/150‑1 (MQ=255)
cGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAAt                                                                                                                                                      <  1:964753/150‑1 (MQ=255)
 gTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCACTGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATc                                                                                                                                                     <  1:5330008/150‑1 (MQ=255)
 gTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATc                                                                                                                                                     <  1:3728519/150‑1 (MQ=255)
     aTAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGAcc                                                                                                                                                 >  2:2149611/1‑150 (MQ=255)
      tAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAACTCGACcg                                                                                                                                                >  2:598126/1‑150 (MQ=255)
      tAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcg                                                                                                                                                >  1:100363/1‑150 (MQ=255)
      tAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcg                                                                                                                                                <  2:2910510/150‑1 (MQ=255)
       aGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcgt                                                                                                                                               >  1:1958075/1‑150 (MQ=255)
         gTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGTTGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCAGGGATCTCATCGGCATTTGTTTCTGGAGCGGAAACCAAATCGACcgtga                                                                                                                                             <  2:4592281/150‑1 (MQ=255)
          tAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcgtgac                                                                                                                                            >  2:2273614/1‑150 (MQ=255)
          tAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcgtgac                                                                                                                                            >  2:5234577/1‑150 (MQ=255)
               gCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCgtgacgtgac                                                                                                                                       >  1:4582720/1‑150 (MQ=255)
                ccTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACt                                                                                                                                      >  2:743406/1‑150 (MQ=255)
                ccTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACt                                                                                                                                      >  1:4388836/1‑150 (MQ=255)
                  ttctttaTTTTATCTCCCCCGCATCATCTTCAAGGGCGGAAAGGTTTTTAGCGTTTTCACCACAGCGACAGATGTTGCCTCTAATACGTACAGGTATCTCTTCGGCTTTTGTTTTTGGAGCGTAAACAAAATCGACCGTGACGTGACTgg                                                                                                                                    <  2:2411312/144‑1 (MQ=255)
                  ttCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTgg                                                                                                                                    >  2:5309954/1‑150 (MQ=255)
                      tGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAAt                                                                                                                                >  2:5180817/1‑150 (MQ=255)
                        aTTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGTCGTGACTGGGAATGc                                                                                                                              >  1:5478942/1‑150 (MQ=255)
                          tttATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCg                                                                                                                            >  2:5149729/1‑150 (MQ=255)
                          tttATCGCCCCCGCAGCATCTTCAATGGCGGCATGGTTGTTCGCGTAGGCACCCCAGCGACTGTTGTTGCCGCTAATACGTTCAGGGATCTCATCGGCTTTTGTTTCGGGAGCGGAAACCAACTCGACCGTGACGTGACTGGGAATGCCg                                                                                                                            <  1:3617937/150‑1 (MQ=255)
                           ttATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGt                                                                                                                           >  2:220592/1‑150 (MQ=255)
                           ttATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGt                                                                                                                           >  2:4901279/1‑150 (MQ=255)
                             aTCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTcc                                                                                                                         >  1:3126385/1‑150 (MQ=255)
                             aTCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTcc                                                                                                                         >  2:5031108/1‑150 (MQ=255)
                             aTCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTcc                                                                                                                         >  2:3147123/1‑150 (MQ=255)
                             aTCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTcc                                                                                                                         >  2:4109541/1‑150 (MQ=255)
                              tcacccccGCAGCATCTTCAATGGCGGAATGTATTTTAGCGTATGCACCACAGCTACTGTTGTTGCCGCTAATACGTTCAGGTATATCATGGGATGTTGTTTCTGGAGCGGAAACAAACTCGACCGTGACGTGACTGGGAATGCCGTCCt                                                                                                                        <  1:1740153/147‑1 (MQ=255)
                               ctcCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTg                                                                                                                       >  2:1195378/1‑150 (MQ=255)
                                tcCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGACACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGa                                                                                                                      >  2:2919241/1‑150 (MQ=255)
                                  ccccGCAGAATCTTCACGGGCGGAAAGGATGTTCGCGTATGCACCACAGCTACAGTTGTTGCCGCTCATACGTTCCGGTATCTCATCGGCTTTTGTTTCTGGAGCGTAAACCAACTCGACCGTGACGTGACTGGGAATGCCGTCCTGAAt                                                                                                                    <  1:3439457/150‑1 (MQ=255)
                                   cccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAAtc                                                                                                                   >  1:3078685/1‑150 (MQ=255)
                                    ccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAAtct                                                                                                                  >  2:180728/1‑150 (MQ=255)
                                        agcaTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCttt                                                                                                              >  1:2529969/1‑150 (MQ=255)
                                        agcaTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCttt                                                                                                              >  1:2402618/1‑150 (MQ=255)
                                        agcaTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCttt                                                                                                              >  1:5523491/1‑150 (MQ=255)
                                        agcaTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCttt                                                                                                              >  1:1506790/1‑150 (MQ=255)
                                           aTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAg                                                                                                           >  2:4868211/1‑150 (MQ=255)
                                            tCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGc                                                                                                          >  2:4552646/1‑150 (MQ=255)
                                            tCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGc                                                                                                          >  2:4298080/1‑150 (MQ=255)
                                             cTTCATTGGCGGAAAGGATGTTAGCTTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCTGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa                                                                                                         <  2:1919230/150‑1 (MQ=255)
                                             cTTCATGGGCGGCATGGATGTTAGCGTATGCACCACCGCGACTGTTGTTGCCGCTCATACGTTCCCGGATCTCATCGGCTGTTGTTTCTGGCGCGTAAACAAACTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa                                                                                                         <  1:595031/150‑1 (MQ=255)
                                             cTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa                                                                                                         >  2:885924/1‑150 (MQ=255)
                                                       ggcAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACtga                                                                                               <  2:301851/150‑1 (MQ=255)
                                                       ggaatggATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCAGGGATCTCATGGGCAGTTGTTTCTGGAGCGGAAACAAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACtga                                                                                               <  1:189265/145‑1 (MQ=255)
                                                         cAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACtgatg                                                                                             >  1:4172869/1‑150 (MQ=255)
                                                          aaGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACtgatga                                                                                            >  2:5534217/1‑150 (MQ=255)
                                                          aaGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACtgatga                                                                                            <  2:752086/150‑1 (MQ=255)
                                                           tggATGTTAGCGTATGCACCACAGCGACAGTTGTGGCCGCTCATACGTTCCGGGATCTCATCGGCATTTTTTTCTGGAGCGTAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAg                                                                                           <  1:723087/149‑1 (MQ=255)
                                                            ggATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGc                                                                                          >  1:2365776/1‑150 (MQ=255)
                                                             gATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCa                                                                                         >  2:2370854/1‑150 (MQ=255)
                                                                gTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGACTGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAt                                                                                      >  2:930177/1‑150 (MQ=255)
                                                                gTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGACTGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAt                                                                                      >  2:2103201/1‑150 (MQ=255)
                                                                gTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAt                                                                                      >  1:4509796/1‑150 (MQ=255)
                                                                gTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTGTTAGCACCGCTCCTGATGAGCACAt                                                                                      >  2:3185474/1‑150 (MQ=255)
                                                                 tttgcttATGCACCACAGCGAATTTTGTTGCCGATAATACTTTCCGGTATCTCTTGGGCAGTTGTTTCTGTAGCGTAAACCAACTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAtt                                                                                     <  1:5374429/144‑1 (MQ=255)
                                                                   aGCGTATGCACCACAGCGACAGTTGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTg                                                                                   <  2:4329054/150‑1 (MQ=255)
                                                                   aGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTg                                                                                   >  2:4756486/1‑150 (MQ=255)
                                                                    gCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGc                                                                                  >  2:3872300/1‑150 (MQ=255)
                                                                       tATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCg                                                                               >  2:4547204/1‑150 (MQ=255)
                                                                          gCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAg                                                                            <  2:4528934/150‑1 (MQ=255)
                                                                            accacAGCGACAGATGTTGCCGCTCATACGTTCACGGATATCATCGGCAGTTGTTTCTGGAGCGGAAACAAACTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGt                                                                          <  2:3297763/150‑1 (MQ=255)
                                                                               acaGCGACTTATGTTGCCGCAAATACGTTCAGGTATCTCTTGGGCTGTTGTTTCTGGAGCGTAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCa                                                                       <  1:2038287/150‑1 (MQ=255)
                                                                                caGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAg                                                                      >  2:1522305/1‑150 (MQ=255)
                                                                                 aGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGt                                                                     >  2:2715681/1‑150 (MQ=255)
                                                                                 aGCGACAGATGTTGCCGCTAATACGTTCAGGGATCTCATGGGCTGTTTTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGt                                                                     <  1:4029160/150‑1 (MQ=255)
                                                                                 aGCGACAGATGTTGCCGCTAATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGt                                                                     <  1:263969/150‑1 (MQ=255)
                                                                                   cGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAg                                                                   >  1:3396932/1‑150 (MQ=255)
                                                                                   cGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAg                                                                   >  1:1826536/1‑150 (MQ=255)
                                                                                    gACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGc                                                                  >  2:5017464/1‑150 (MQ=255)
                                                                                     aCAGATGTTGCCGCTCATACGTTCACGTATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGcc                                                                 <  2:3485412/150‑1 (MQ=255)
                                                                                        gATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCa                                                              >  1:1090965/1‑150 (MQ=255)
                                                                                           gtggCCGCTCATACGTTCCCGTATCTCATCGGCTGTTGTTTCTGGCGCGTAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTg                                                           <  1:1594195/147‑1 (MQ=255)
                                                                                               ccGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAg                                                       >  2:4710745/1‑150 (MQ=255)
                                                                                                     aTACGTTCACGGAACTCATCGGCAGTTGTTACTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca                                                 <  2:2304293/150‑1 (MQ=255)
                                                                                                      tACGTTCACGGATCTCATCGGCTGTTGTTTCGGGAGCGGAAACCAAAGCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatcan                                                <  2:1386551/150‑2 (MQ=255)
                                                                                                         gTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCt                                             >  1:1200832/1‑150 (MQ=255)
                                                                                                           tCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTg                                           >  1:883284/1‑150 (MQ=255)
                                                                                                             aCGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGAt                                         >  2:3746013/1‑150 (MQ=255)
                                                                                                              gggATCTCATCGGCTGTTGTTTCTGGAGCGGAAACAAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATa                                        <  1:1379047/149‑1 (MQ=255)
                                                                                                                taTCTCATCGGCTGTTTTTTCTGGAGCGTAAACAAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATaaa                                      <  1:4736397/149‑1 (MQ=255)
                                                                                                                  tctcATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATaaatg                                    <  2:2529969/150‑3 (MQ=255)
                                                                                                                  tatcATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAgg                                    <  1:1792502/148‑1 (MQ=255)
                                                                                                                   ctcATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgc                                   >  2:1014349/1‑150 (MQ=255)
                                                                                                                     cATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgccg                                 >  1:3218210/1‑150 (MQ=255)
                                                                                                                       tCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgccgcc                               >  1:4208797/1‑150 (MQ=255)
                                                                                                                         ggCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTg                             <  2:5364659/150‑1 (MQ=255)
                                                                                                                          gCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGc                            >  2:1835238/1‑150 (MQ=255)
                                                                                                                           cAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAATGCCGCCTGcc                           >  2:4368127/1‑149 (MQ=255)
                                                                                                                              ttgttTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATgg                        >  1:4626974/1‑150 (MQ=255)
                                                                                                                                gttTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATgggg                      >  2:1345237/1‑150 (MQ=255)
                                                                                                                                   tcgggAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGa                   <  1:4966105/147‑1 (MQ=255)
                                                                                                                                     tGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAg                 <  1:184859/150‑1 (MQ=255)
                                                                                                                                       gAGCGTAAACCAACTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGAt               <  1:5112415/150‑1 (MQ=255)
                                                                                                                                        aGCGTAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGAtt              <  1:2224265/150‑1 (MQ=255)
                                                                                                                                          cGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTAt            <  1:4441748/150‑1 (MQ=255)
                                                                                                                                             aaaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTg         >  1:732175/1‑150 (MQ=255)
                                                                                                                                             aaaCAAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTg         <  1:1671464/150‑1 (MQ=255)
                                                                                                                                              aaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTgg        >  1:2323559/1‑150 (MQ=255)
                                                                                                                                                  aaaTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGaa    >  1:3857232/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGGTGAAGATTATCTGGCGAACC  >  CP009273/297576‑297873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑