Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,333 A→T 9.8% intergenic (+88/‑256) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,3330AT 9.8% 831.7 / 13.7 281intergenic (+88/‑256)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base A (90/149);  new base T (19/2);  total (130/151)
Fisher's exact test for biased strand distribution p-value = 2.17e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

AAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTG  >  CP009273/450184‑450479
                                                                                                                                                       |                                                                                                                                                   
aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGa                                                                                                                                                     <  2:4388280/150‑1 (MQ=255)
aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGa                                                                                                                                                     <  2:4784834/150‑1 (MQ=255)
aaGGGGTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGa                                                                                                                                                     <  2:5084431/150‑1 (MQ=255)
 aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTT‑TCTGGAt                                                                                                                                                    >  2:3580866/1‑150 (MQ=255)
 aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGAt                                                                                                                                                    >  2:2657069/1‑150 (MQ=255)
  gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGAtt                                                                                                                                                   <  1:5406581/150‑1 (MQ=255)
  gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGAtt                                                                                                                                                   <  1:5421923/150‑1 (MQ=255)
   ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGAttt                                                                                                                                                  <  1:1753538/150‑1 (MQ=255)
   ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGAttt                                                                                                                                                  <  1:4336441/150‑1 (MQ=255)
   ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TATGGAttt                                                                                                                                                  >  2:5321940/1‑150 (MQ=255)
   ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TCTGGCttt                                                                                                                                                  >  2:4378242/1‑150 (MQ=255)
      tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTgc                                                                                                                                               <  2:702601/150‑1 (MQ=255)
       gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTgcg                                                                                                                                              <  1:2798318/150‑1 (MQ=255)
       gCAGGACACCGTCTTTGCCTCTCCTCCCGGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGTTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTgcg                                                                                                                                              <  1:1005923/150‑1 (MQ=255)
          ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TGTTGAgttttgggtt                                                                                                                                           >  1:1072329/1‑144 (MQ=255)
          ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TCTGGATTTTtcgcat                                                                                                                                           >  1:1322144/1‑148 (MQ=255)
             cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCttttt                                                                                                                                        <  2:236316/150‑1 (MQ=255)
             cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCttttt                                                                                                                                        <  1:2964074/150‑1 (MQ=255)
               ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTT‑TCTGGTTTTTGGGCttttttc                                                                                                                                      >  2:3767086/1‑148 (MQ=255)
                 gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGccc                                                                                                                                    >  2:392977/1‑150 (MQ=255)
                 gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TGTGGATTTTTGGCTTTTTGccc                                                                                                                                    >  2:1427473/1‑150 (MQ=255)
                  tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCa                                                                                                                                   <  1:4957352/150‑1 (MQ=255)
                  tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCa                                                                                                                                   <  2:1014929/150‑1 (MQ=255)
                     ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TCTGGTTTTTTCGCTTTTTTGCCAGcc                                                                                                                                >  1:4951034/1‑149 (MQ=255)
                     ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TCTGGATTTTTCGCTTTTTTCCCAGCa                                                                                                                                >  1:3081659/1‑150 (MQ=255)
                      tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                                                               <  1:695916/150‑1 (MQ=255)
                       gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTTTGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                                                              <  1:3296190/150‑1 (MQ=255)
                        ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                                             <  1:805023/150‑1 (MQ=255)
                         ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTT‑TGTGGTTTTTTGGCTTTTTGCCCAGCATTCa                                                                                                                            >  1:2727375/1‑150 (MQ=255)
                           ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                          <  1:2115711/150‑1 (MQ=255)
                            tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAc                                                                                                                         <  1:2285905/150‑1 (MQ=255)
                             cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACg                                                                                                                        >  2:3505050/1‑150 (MQ=255)
                                cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCAATCAGTCG‑aaa                                                                                                                    >  1:3685085/1‑150 (MQ=255)
                                   gggcggggAGCCGGAAGCAACGCGTAAAAACGCATTTGCAACTGTCGGCGCATTCCCAGTATGTTGCTAAAGATTTTATGAAACACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAtt                                                                                                                 <  2:1686162/144‑1 (MQ=255)
                                    gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TGTGGATTTTGCGCTTTTTTCCCAGCATTAAGACG‑AAAATTg                                                                                                                >  2:1094884/1‑150 (MQ=255)
                                    gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGA‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTg                                                                                                                <  2:2244682/150‑1 (MQ=255)
                                      cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGcc                                                                                                              <  2:4126206/150‑1 (MQ=255)
                                          gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCggg                                                                                                          <  1:3010812/150‑1 (MQ=255)
                                           aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                                                                         <  1:1107328/150‑1 (MQ=255)
                                             cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAt                                                                                                       <  2:2735410/150‑1 (MQ=255)
                                              aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCAGGATTTTGCGCTATTTGCCCACCATTCACACG‑AAAATTGCCCGGGaagt                                                                                                      >  2:2286878/1‑148 (MQ=255)
                                              aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAATACGGCTTGCGGGCCGT‑TTTTTTTTT‑TGTGGATTTTTGGGTTTTTGCCCAGCATACATACG‑TAAATTTCCCGGGAAtt                                                                                                      >  2:2904138/1‑150 (MQ=255)
                                              aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt                                                                                                      <  1:4892380/150‑1 (MQ=255)
                                               ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtg                                                                                                     <  2:3374089/150‑1 (MQ=255)
                                                 aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtgtg                                                                                                   <  1:5502719/150‑1 (MQ=255)
                                                  aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGa                                                                                                  >  2:5493393/1‑150 (MQ=255)
                                                   gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTGTTTTGGTTTTTGGGTTTTTTGGCAGACATAAGAACG‑AAAATTCCCCGGGAAtgttta                                                                                                  >  1:4316896/1‑145 (MQ=255)
                                                   gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTG‑TCTGGTTTTTGAGCTTTTTTCCCAGCAATCATAAG‑AAAAATGACCCGGCAtttttta                                                                                                 >  2:945500/1‑145 (MQ=255)
                                                    cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTT‑TGGGGATTTTTAGGTTTTTTCCCAGCAATAAGGCG‑AAAAATGCCCGGGAAATGTGtaa                                                                                                >  1:2715956/1‑150 (MQ=255)
                                                       cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTTTTTTT‑TGGGGGTTTTTGGCTTTTTTCCCAGCAATGAGTCG‑GAAATTTCCCGGGGATTTTGaaaaaa                                                                                             >  1:1283321/1‑150 (MQ=255)
                                                         cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATa                                                                                           <  1:3772618/150‑1 (MQ=255)
                                                         cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATa                                                                                           <  2:4779474/150‑1 (MQ=255)
                                                          gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCAATCAGACG‑AAAATTGCCCGGGCATTGTGAAAAAATAc                                                                                          >  2:3062820/1‑150 (MQ=255)
                                                            aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                                        >  2:790434/1‑150 (MQ=255)
                                                            aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TATGGATTTTTCGCTTTTTTGCCAGCAATCAGACG‑AAAATTTCCCGGGCAATTTGAAAAAATAagc                                                                                        >  2:1048438/1‑150 (MQ=255)
                                                                  gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTTT‑TTTGGTTTTTGGGGTTTTTGCCC‑GCAAGAAGAAGCAAAATTGCCCGGGGATGTGGAAAAAAAAcacacaac                                                                                   >  1:2392980/1‑143 (MQ=255)
                                                                   cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTG‑TCTTGATTTTGGGCTTTTTGCCCCGCAATCAGACG‑AAAATTTCCCGGGCAGTGTGAAAAAATAAGCGCCAgcg                                                                                 >  2:3753048/1‑150 (MQ=255)
                                                                      ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACG‑AAAATTTCCCGGGAATTGTGAAAAAATACGCGAAAgcgcgc                                                                              >  2:3143083/1‑150 (MQ=255)
                                                                         cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                                                                           <  1:410019/150‑1 (MQ=255)
                                                                               tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTT‑TATTGATTTTTGGCTTTTTTACCACCAATAAGTAG‑AAAAATTCCCGGGAATTGTTATAAAATAAGCGAAATCGCGCAAAAAccct                                                                     >  1:3674114/1‑148 (MQ=255)
                                                                                 ggCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTT‑TATGGATTTTTAGCTTTTTTCCCAACAATAAGACG‑AAAAATGCCCGGGAATTGTGTAAAAAAAACCGACAGCGCGCAaaaaacatta                                                                   >  1:2632358/1‑144 (MQ=255)
                                                                                   cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                 <  1:5522383/150‑1 (MQ=255)
                                                                                    gcTTTTCAATTTTGTTGATAAAGATTTTATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                                <  1:973573/150‑1 (MQ=255)
                                                                                                tGTTGCTAATGTTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                    <  1:2887192/150‑1 (MQ=255)
                                                                                                tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                    <  1:858570/150‑1 (MQ=255)
                                                                                                tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                    <  1:2579695/150‑1 (MQ=255)
                                                                                                      tAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                              <  2:3703142/150‑1 (MQ=255)
                                                                                                           aTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTTCGCGTTTTGCCCAGCATTCAGACG‑AAAAATGCCCGGGAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCAAAAAATTGATTCCGCgat                                         >  2:163986/1‑150 (MQ=255)
                                                                                                           aTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtat                                         <  2:2563395/150‑1 (MQ=255)
                                                                                                           aTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCACCATTCAGACG‑AAAATTGCCCCGGAAATGTGAAAAAAAACCCGACACCGCGCAAAAACCGTTCACGACTCAAAAAAGTGATGCCGCtat                                         >  2:1283582/1‑150 (MQ=255)
                                                                                                            ttttATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtata                                        <  1:5494350/150‑1 (MQ=255)
                                                                                                            ttttATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGATTTTGGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtata                                        <  1:2072598/150‑1 (MQ=255)
                                                                                                            ttttATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTT‑TGTGGAGTTTGGGCTTTTTTCCCCGCCAACAGACG‑AAAATTGCCCCGGAAATGTGAAAAAAAACGCGACAGCGCGCAAAAAACCTTCTCGACTCATAAAAGTGATGCCGCgata                                        >  2:1977197/1‑150 (MQ=255)
                                                                                                             tttATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCAGTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATaa                                       <  2:3440171/150‑1 (MQ=255)
                                                                                                                aTGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTT‑TCTGGATTTTGCGCATTTTTCCCCGCAATCCGACG‑AAAAATTCCCGGGAATTGTGAAAAAATAAACGACCCCGCGCAAAAAACCTTCTCGACACATAAAATTGAGTCCGCGATAATGc                                    >  2:4189418/1‑150 (MQ=255)
                                                                                                                 tGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTT‑TCTGGATTTTTGGCTTTTTTCCCCGCAATCAGACG‑AAAAATGCCCCGGCATTGTGAAAAAATAAGCGACACCGCGCCAGAACCGTTCTCGACTCATAAAATTGTTGCCGCTATAATGcc                                   >  2:2782051/1‑150 (MQ=255)
                                                                                                                  gAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAACGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                  >  2:4566555/1‑150 (MQ=255)
                                                                                                                    aaaaCGGCCTGCGGGCCGT‑GTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAATGCCCGGGAATTGTGTAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                >  2:890488/1‑150 (MQ=255)
                                                                                                                     aaaCGGCCTGCGGGCCGT‑TTTGTTTTT‑TCTGGATTTTTCGCTTTTTTCCCAGCCATCAGAAG‑AAAAATTCCCGGGCATTGTGAAAAAATAAGCGACAGCCCGCAAAAACCGGTCTCGACTCATAAAATTGATGACGCGAGAATGCcgcgg                               >  2:4808222/1‑149 (MQ=255)
                                                                                                                       aCGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc                             <  2:148587/150‑1 (MQ=255)
                                                                                                                         ggCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTa                           <  2:1870497/150‑1 (MQ=255)
                                                                                                                           ccTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                         <  2:2157360/150‑1 (MQ=255)
                                                                                                                            cTGCGGGCCGT‑TTTGTTTTT‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAAAAACGTTCTCGACACATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                        >  2:1777862/1‑150 (MQ=255)
                                                                                                                                gggCCGT‑TTTGTTTTG‑TCTGGATTTTTCGCTTTTTTCCCAGCATTCAGACG‑AAAAATTCCCGGGAATTGTGAAAAAATAAGCGACCGCGCGCAATAACCGTTATCGACACATAAAAGTGATTCCGCTATAATGCCGCGTCCTATTTTaaa                    >  2:4107404/1‑149 (MQ=255)
                                                                                                                                   ccGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt                 <  1:5352640/150‑1 (MQ=255)
                                                                                                                                    cGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                >  1:1685438/1‑150 (MQ=255)
                                                                                                                                    cGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                <  2:1193111/150‑1 (MQ=255)
                                                                                                                                      t‑tttgttttg‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGACTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc              >  2:2119314/1‑150 (MQ=255)
                                                                                                                                      t‑tttgttttg‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc              >  1:5112599/1‑150 (MQ=255)
                                                                                                                                         ttgttttg‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg            >  1:4991152/1‑150 (MQ=255)
                                                                                                                                           gttttg‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga          >  2:496217/1‑150 (MQ=255)
                                                                                                                                           gttttg‑tCTGGATTGTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga          <  1:3927788/150‑1 (MQ=255)
                                                                                                                                            ttttg‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat         <  1:3767069/150‑1 (MQ=255)
                                                                                                                                             tttg‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg        >  1:3379087/1‑150 (MQ=255)
                                                                                                                                             tttg‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg        <  2:5132214/150‑1 (MQ=255)
                                                                                                                                             tttg‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg        <  1:4566969/150‑1 (MQ=255)
                                                                                                                                                g‑tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt     <  2:2935874/150‑1 (MQ=255)
                                                                                                                                                  tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc    <  2:4877590/150‑1 (MQ=255)
                                                                                                                                                   cTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt   <  2:5158063/150‑1 (MQ=255)
                                                                                                                                                   cTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGCTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt   <  2:3905669/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
AAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTG‑TCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTG  >  CP009273/450184‑450479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑