Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 772,664 A→C 12.5% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273772,6640AC12.5% 509.5 / 13.8 190K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (135/26);  new base C (2/27);  total (137/53)
Fisher's exact test for biased strand distribution p-value = 6.37e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

CAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATA  >  CP009273/772516‑772812
                                                                                                                                                      |                                                                                                                                                     
cagAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑g                                                                                                                                                     >  2:1124967/1‑150 (MQ=255)
 agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑Gc                                                                                                                                                    >  1:467815/1‑150 (MQ=255)
 agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑Gc                                                                                                                                                    >  1:2393399/1‑150 (MQ=255)
 agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGACGCCGCAGACAAAAAAG‑AGGCAGCGGCACAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑Gc                                                                                                                                                    >  1:3228769/1‑150 (MQ=255)
            cAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAgg                                                                                                                                         >  2:175995/1‑150 (MQ=255)
             aGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGc                                                                                                                                        >  1:2659767/1‑150 (MQ=255)
                 gACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAgag                                                                                                                                    >  2:2429221/1‑149 (MQ=255)
                  aCAAAAAAGAATCTGAAAAAGCGGCTGCTGAAAAGGCTGATGCTGATACGACTGCAGCGGCATAAAACGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAC‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAgatg                                                                                                                                   <  2:426312/150‑1 (MQ=255)
                  aCAAAAAACCAGCATAAAAGGGGGGTGATTAAAAGGAAGCTGATTATATTAAACCTGGGTCATAAAAAGCCGCCGAATAAAAAAAAG‑CGGAAGCGGAAAAA‑GCTGAAGCTGAAAAAGCCGCTGCAGAAAAA‑GCGGCCGCAGAGGCAgatg                                                                                                                                   <  2:5247283/150‑1 (MQ=255)
                      aaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGatat                                                                                                                               >  1:3569888/1‑150 (MQ=255)
                       aaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATAtt                                                                                                                              >  1:261819/1‑150 (MQ=255)
                       aaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATAtt                                                                                                                              >  1:4831970/1‑150 (MQ=255)
                       aaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATAtt                                                                                                                              >  1:4049038/1‑150 (MQ=255)
                       aaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGAGAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACACAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATAtt                                                                                                                              >  2:1121989/1‑150 (MQ=255)
                        aaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATAttt                                                                                                                             >  2:4507385/1‑150 (MQ=255)
                        aaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATAttt                                                                                                                             >  2:582171/1‑150 (MQ=255)
                        aaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATAttt                                                                                                                             >  2:389455/1‑150 (MQ=255)
                           cagcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCg                                                                                                                          >  2:1667534/1‑150 (MQ=255)
                            agcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCACAA‑GCGGCCGCAGAGGCAGATGATATTTTCgg                                                                                                                         >  2:4407445/1‑150 (MQ=255)
                                 aaaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCACAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGc                                                                                                                    >  1:3154573/1‑150 (MQ=255)
                                 aaaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGc                                                                                                                    >  1:3154607/1‑150 (MQ=255)
                                  aaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt                                                                                                                   >  2:4414616/1‑150 (MQ=255)
                                  aaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt                                                                                                                   >  2:794976/1‑150 (MQ=255)
                                    aaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                 >  1:2332856/1‑150 (MQ=255)
                                     aGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                >  2:1718148/1‑150 (MQ=255)
                                         gctgctTAAACGGCAGCTGATTATAATCAAGCTGCGGAATAAAAAGCCGCCGCACACAAAAAAG‑CGGCAGCGGAAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGctct                                                                                                            <  2:2191008/150‑1 (MQ=255)
                                           tgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTgg                                                                                                          >  2:190864/1‑150 (MQ=255)
                                             cttaaaaGCCAGCAGCTGATAACAAAGCAGCGGCAGAAAAACCCGCCGCAGACAAAAAAG‑CGGAAGCGGAAAAA‑GCTGCTGCTCAAAACCCCGCTGCAGAAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                        <  2:4032651/147‑1 (MQ=255)
                                             ctgAAAAGGCAGCAGCTGATAAGAAAGTAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                        >  1:2901468/1‑150 (MQ=255)
                                             ctgAAAAGCCAGCATCTGATAAGAACGCAGCGGCAGAAAAAGCCGCCGCAGAAAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                        <  2:1268872/150‑1 (MQ=255)
                                               gAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                      >  1:4186618/1‑150 (MQ=255)
                                               gAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                      >  1:5074578/1‑150 (MQ=255)
                                                 aaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGTTATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                    >  2:4114705/1‑150 (MQ=255)
                                                  aaGGCAGCAGCTGATAAGACACCAGCGGCAGAAAACGCCGCAGCAGACAAAAAAG‑CGCCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAt                                                                                                   <  2:3163885/150‑1 (MQ=255)
                                                  aaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTAGGTGAGCTAAGCTCTGGTAAGAAt                                                                                                   >  2:939648/1‑150 (MQ=255)
                                                   aGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATg                                                                                                  >  1:3872445/1‑150 (MQ=255)
                                                      cagcagCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAc                                                                                               >  1:2228928/1‑150 (MQ=255)
                                                        gcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAAAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAACGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCg                                                                                             <  2:1343433/150‑1 (MQ=255)
                                                          agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                           >  2:2771528/1‑150 (MQ=255)
                                                          agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                           >  1:2987970/1‑150 (MQ=255)
                                                          agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                           >  1:4118064/1‑150 (MQ=255)
                                                           gcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaaa                                                                                          >  2:2439974/1‑150 (MQ=255)
                                                            cTGATAACACAGCCGCGGCAGAAAAAGCCGCCGCACACAAAAACG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaaaa                                                                                         <  2:457588/150‑1 (MQ=255)
                                                             tGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAAc                                                                                        >  2:752211/1‑150 (MQ=255)
                                                             tGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAAc                                                                                        >  2:1426498/1‑150 (MQ=255)
                                                                 aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCACAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                    >  2:3093970/1‑150 (MQ=255)
                                                                 aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                    >  1:5393283/1‑150 (MQ=255)
                                                                  aGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGACAACggggg                                                                                   >  2:4449328/1‑150 (MQ=255)
                                                                   gAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggggg                                                                                  >  2:2853717/1‑150 (MQ=255)
                                                                    aaaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGa                                                                                 >  1:3421646/1‑150 (MQ=255)
                                                                        ctggggCAAAAAACGCGTCCGAACAAAAAAACG‑CGGCAGCGCAAAAA‑GCTGCTGATTAAAAAGCCGGTCCACAAAAC‑GCGGCCGCAGAGGCAGATTATTTTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAgggg                                                                             <  2:1574770/146‑1 (MQ=255)
                                                                        cagcGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAgggg                                                                             >  2:699053/1‑150 (MQ=255)
                                                                         cgcgcCATAAAACGCCGCC‑CAAAAAAAAACGCCGGCCGCGCAAAAA‑GGGGCTTCTCAAAACCCCGGTGCACAAAAC‑GCGGCCGCAGAGGCAGATTATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGc                                                                            <  2:2541073/149‑1 (MQ=255)
                                                                         agcGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGc                                                                            >  1:4924138/1‑150 (MQ=255)
                                                                         agcGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCGGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGc                                                                            >  2:3487247/1‑150 (MQ=255)
                                                                            ggCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaa                                                                         >  1:2720901/1‑150 (MQ=255)
                                                                            ggCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaa                                                                         >  1:991929/1‑150 (MQ=255)
                                                                             gCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaaa                                                                        >  1:986119/1‑150 (MQ=255)
                                                                              cAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTCAGAATGCACCGAAAACGGGGGGAGGGGCGAAAg                                                                       >  2:2918316/1‑150 (MQ=255)
                                                                               aaaaaacGCCCCCGCACAAAAAAACG‑CGGCTGCGCAAAAC‑GCTGCAGCTTAAAACCCCGCTGCACCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                      <  2:2653677/148‑1 (MQ=255)
                                                                               aGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                      >  2:3165356/1‑150 (MQ=255)
                                                                               aGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                      >  2:3500838/1‑150 (MQ=255)
                                                                               aGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                      >  1:3847245/1‑150 (MQ=255)
                                                                                       ccgccgCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATg                                                              >  1:4073317/1‑150 (MQ=255)
                                                                                           cgcAGCAAACAACG‑CGCCAGCGGAAAAC‑GCAGCAGCTTAAAACGCCGCTG‑ACCAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                          <  2:84678/150‑1 (MQ=255)
                                                                                           cgcAGAAAAAAACG‑CGGCAGCGGAAAAA‑GCTGAAGCTTAAAAAGCCGCTGCTCAAAAA‑GCGGCCGCAGCGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                          <  2:1800400/150‑1 (MQ=255)
                                                                                            taacaaaaaaacG‑CGGCTG‑GGCAAAACGCTCCAGTTTAAAACCCCGCTGCAGAAAAC‑GCGGCCGCAGTGGCTGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCg                                                         <  1:346971/144‑1 (MQ=255)
                                                                                            gcAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTCAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCg                                                         >  2:5080058/1‑150 (MQ=255)
                                                                                             cAGACAAAAAAG‑CGGCAGCGGCACAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGAGGATATTTTCGGTGAGCTCAGCTCTGGTAAGACTGCACCGAAAACGGGGGGAGGGGCGACAGGGACCAATGCGTCGc                                                        >  2:4472236/1‑150 (MQ=255)
                                                                                              aGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                       >  1:3637737/1‑150 (MQ=255)
                                                                                              aGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                       >  1:3704753/1‑150 (MQ=255)
                                                                                              aGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                       >  1:3776046/1‑150 (MQ=255)
                                                                                               gaaaaaaacG‑CGGCTGCGGCAACC‑GGTGCAGCTGAAAAAGCCGGTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                      <  2:3392475/147‑1 (MQ=255)
                                                                                                aCAAAAAAG‑CGGCCGCGGCAACC‑GGGGCTGCTTAAACAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTg                                                     <  2:4354321/150‑1 (MQ=255)
                                                                                                  aaaaaaG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGACCAATGCTTCGCCTGcc                                                   >  1:109683/1‑150 (MQ=255)
                                                                                                  aaaaaaG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  1:2061220/1‑150 (MQ=255)
                                                                                                  aaaaaaG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  1:5107806/1‑150 (MQ=255)
                                                                                                   aaaatG‑CGGCCGCGGAAAAA‑GCTGCTGCTTAACACGCCGGTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCg                                                  <  2:798096/150‑1 (MQ=255)
                                                                                                   aaaacG‑CGGCAGCGGCAAAT‑GCTGCAGCTTAAAACGCCGCTGAAGAAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCg                                                  <  2:797925/150‑1 (MQ=255)
                                                                                                      atg‑cggcagctgaaAAA‑GCTCCAGTTGAAAAAGCCGCTGCAGCAACA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                               <  2:2107243/148‑1 (MQ=255)
                                                                                                          cggcagcggcaAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                            >  1:4396854/1‑150 (MQ=255)
                                                                                                          cggcagcggcaAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                            >  1:593769/1‑150 (MQ=255)
                                                                                                           ggcagcggcaAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAgggg                                           >  2:3537690/1‑150 (MQ=255)
                                                                                                                    aaaa‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                  >  2:5269058/1‑150 (MQ=255)
                                                                                                                     aaa‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                 >  2:3230426/1‑150 (MQ=255)
                                                                                                                       aggctgctgctAAAACCGCCGCTGCCGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaa                                <  2:2412326/148‑1 (MQ=255)
                                                                                                                       a‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGTACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                               >  2:3575061/1‑150 (MQ=255)
                                                                                                                       a‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                               >  1:4295126/1‑150 (MQ=255)
                                                                                                                         gCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAAc                              >  1:5284802/1‑150 (MQ=255)
                                                                                                                          cTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACa                             >  1:39489/1‑150 (MQ=255)
                                                                                                                                   aaaaaGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                    >  1:1552377/1‑150 (MQ=255)
                                                                                                                                     aaaGCCGCGGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                  <  2:4131247/150‑1 (MQ=255)
                                                                                                                                      acgCCGCTGCAGAAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAg                 <  2:3421646/148‑1 (MQ=255)
                                                                                                                                       aGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                >  1:1029794/1‑150 (MQ=255)
                                                                                                                                         ccGCTGCAGAAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgggg              <  2:2300102/150‑1 (MQ=255)
                                                                                                                                         ccGCTCCAAAAAAC‑GCGGCCGAAGAGGCATATTTTATTTTCGTTGTCCTAAGATATTGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgggg              <  1:2104503/150‑1 (MQ=255)
                                                                                                                                           gCTGCAGCACAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGcc            >  2:3806407/1‑150 (MQ=255)
                                                                                                                                             tGCAGAAAAC‑GCGGCCGCAGAGGCTGATGATATTGTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGa          <  2:2016913/150‑1 (MQ=255)
                                                                                                                                                   aaaa‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTCAAAACAATGGCGCATCAGGGGCCGATATCaa    >  2:4098085/1‑150 (MQ=255)
                                                                                                                                                   aaaa‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa    >  1:226117/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAG‑CGGCAGCGGCAAAA‑GCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATA  >  CP009273/772516‑772812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑