Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,784,723 A→C 9.6% F260C (TTT→TGT)  ydiU ← UPF0061 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,784,7230AC 9.6% 710.4 / 10.3 249F260C (TTT→TGT) ydiUUPF0061 family protein
Reads supporting (aligned to +/- strand):  ref base A (152/66);  new base C (2/29);  total (154/95)
Fisher's exact test for biased strand distribution p-value = 7.84e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

GCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATC  >  CP009273/1784576‑1784871
                                                                                                                                                   |                                                                                                                                                    
gCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAAc                                                                                                                                                    >  1:3824802/1‑150 (MQ=255)
  aTCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACgg                                                                                                                                                  >  2:5333125/1‑150 (MQ=255)
  aTCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACgg                                                                                                                                                  >  1:2959545/1‑150 (MQ=255)
   tCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGc                                                                                                                                                 >  2:3331184/1‑150 (MQ=255)
    cTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGcc                                                                                                                                                >  2:4568341/1‑150 (MQ=255)
    cTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGcc                                                                                                                                                >  2:3622253/1‑150 (MQ=255)
     tACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGccc                                                                                                                                               >  1:1012301/1‑150 (MQ=255)
          caacaaATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAAt                                                                                                                                          >  2:1574335/1‑150 (MQ=255)
              aatgggCGACACGGTCTGCGCCAGACGCTGTAAATTCCACACCGCGACGGCTGGTTGATTATCAATGCTGTAACGCCCTTGATGATCCGAGTTATTACAAATAAAACCGGGTTCGTAATCATCAATAAAACCAAACGGCCCGTAATCAAg                                                                                                                                      <  2:3567787/146‑1 (MQ=255)
                aTGGCGACATTGTATGCGCCAGACGCTTTAAATTCCACAACGCGAGGGATGGTTGTTTATCAAAGATTTAACGCCCTTTATTATCCTTGTTTTTAAAAATAAAACCGGGTTCGTAAAAATAAAGAAAACCAAACGGCCCGTAATCAAGCg                                                                                                                                    <  2:57168/150‑1 (MQ=255)
                aTGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCg                                                                                                                                    >  2:3983331/1‑150 (MQ=255)
                     gACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGc                                                                                                                               >  1:1630873/1‑150 (MQ=255)
                      aCAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTACTCAAGCGTCAGcc                                                                                                                              >  2:341052/1‑150 (MQ=255)
                      aCAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGcc                                                                                                                              >  2:340347/1‑150 (MQ=255)
                        actGTCTGCGCCAGACGCTTTAAATTCCACACCGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGcccc                                                                                                                            <  2:2254121/148‑1 (MQ=255)
                        aaTGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTCATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGcccc                                                                                                                            >  1:3327149/1‑150 (MQ=255)
                        aaTGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGACAGcccc                                                                                                                            >  1:358123/1‑150 (MQ=255)
                            tCTGCGCCAGACGCTGTAAATTCCACACCGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCcagca                                                                                                                        <  2:307183/150‑1 (MQ=255)
                                cgcCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGa                                                                                                                    >  2:2517368/1‑150 (MQ=255)
                                   cAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAACCCGGGTTCGTAATCATCAATAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAt                                                                                                                 <  2:264427/150‑1 (MQ=255)
                                    aGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAtg                                                                                                                >  1:1005031/1‑150 (MQ=255)
                                       cGCTTTAAATTCCACACCGCGAGGGCTGTTTTTTTATCAATGCTGTAACGCCCTTTTTTATCCGTGTGATTAAAAATAAAACGGGTTTCTTAATAATAAATAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAtgttg                                                                                                             <  1:1704081/150‑1 (MQ=255)
                                       cGCTTTAAATACCACACCGCTACGGCAGGTTTATTAACAACGCTTAACCGCCCTTGATGATCCGTGTTATAAAAAATAAAACGGGTTTCGTAAAAATCAATAAACCCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAtgttg                                                                                                             <  1:1703627/150‑1 (MQ=255)
                                         cTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTc                                                                                                           >  1:2259301/1‑150 (MQ=255)
                                         cTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTc                                                                                                           >  2:5248213/1‑150 (MQ=255)
                                           gTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCgg                                                                                                         >  1:2169091/1‑150 (MQ=255)
                                            tAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTTTAACGCCCTTGATGATCCGAGTGATTAAAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGt                                                                                                        <  2:4820660/150‑1 (MQ=255)
                                             aaaTTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTa                                                                                                       >  2:3874438/1‑150 (MQ=255)
                                               aTTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTAtt                                                                                                     >  1:4040646/1‑150 (MQ=255)
                                                  ccACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTTATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtcat                                                                                                  >  2:1927137/1‑150 (MQ=255)
                                                  ccACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtcat                                                                                                  >  2:5134714/1‑150 (MQ=255)
                                                     caccgcgACGGCTGGTTGATTATCAAAGCTGTACCGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAc                                                                                               <  1:4125575/147‑1 (MQ=255)
                                                         gcgACGGCTGGTTTATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTAAAAATAAAACCGGGTTCGTAATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCa                                                                                           <  1:870393/150‑1 (MQ=255)
                                                          cgACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCAt                                                                                          >  1:2058545/1‑150 (MQ=255)
                                                                cAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaa                                                                                    >  1:2780715/1‑150 (MQ=255)
                                                                 aGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaaa                                                                                   >  2:2997972/1‑150 (MQ=255)
                                                                 aGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaaa                                                                                   >  2:2998223/1‑150 (MQ=255)
                                                                  gttttaTTATCAAAGCTGTAACGCCCTTGATGATCCGAGTTTTTACAAATAAAACCGGTTTCGTAATAATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAg                                                                                  <  1:403592/145‑1 (MQ=255)
                                                                    ttGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGcc                                                                                >  2:1844147/1‑150 (MQ=255)
                                                                       aTTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGAc                                                                             >  2:5484263/1‑150 (MQ=255)
                                                                        ttATCAAAGCTTTAACGCCCTTGTTGATCCTTTTGATTACAAATAAAACCGGTTTCGTAATAATAAATAAAACAAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGAcc                                                                            <  1:1385144/150‑1 (MQ=255)
                                                                           tCAAAGCTTTAACGCCCTTGATGATCCGTGTGATTACAAATAAAACCGGGTTCGTAATCATAAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTc                                                                         <  1:5019269/150‑1 (MQ=255)
                                                                             aacgCTTTAACGCCCTTGATGATCCGAGTTATTACAAATAAAACCGGGTTCGTAAAAATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTg                                                                       <  2:498068/147‑1 (MQ=255)
                                                                                  tGTAACGCCGTTGATGATCCTTGTGATTACAAATAAACCGGGTTTCGTAATCATCAAGAAACCCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCAtt                                                                  <  1:3084906/150‑1 (MQ=255)
                                                                                  tGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACTCCATGAGCAAAGCCGACCGTCTGCCagt                                                                  >  2:1535367/1‑148 (MQ=255)
                                                                                    tAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTgg                                                                >  2:3085020/1‑150 (MQ=255)
                                                                                     aaCGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTggg                                                               >  1:4540884/1‑150 (MQ=255)
                                                                                        gCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCaa                                                            >  1:2855887/1‑150 (MQ=255)
                                                                                          ccTTTATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAtt                                                          <  2:114082/150‑1 (MQ=255)
                                                                                          ccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAtt                                                          >  2:2491759/1‑150 (MQ=255)
                                                                                               ttgatCCGTGTGATTAAAAATAAAACCGGGGTCGTAATCATCAACAAACCCCCACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAacga                                                     <  1:1463623/149‑1 (MQ=255)
                                                                                                tgatCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAacgac                                                    >  1:4899708/1‑150 (MQ=255)
                                                                                                  atCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCACTTAACGAcgc                                                  >  2:1070097/1‑150 (MQ=255)
                                                                                                     cGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGt                                               >  2:3147937/1‑150 (MQ=255)
                                                                                                     cGAGAGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGCCCCGTAATCAATCGTCAGCCCCAGCAGCGACATGATCTCGGTATTCATCACCCCATGAGCAAAGCCGACCGACTGACATACGGCAATAAACGAcgcgtt                                               >  2:2134885/1‑148 (MQ=255)
                                                                                                      gAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTa                                              <  2:1012301/150‑1 (MQ=255)
                                                                                                       aGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTAc                                             >  1:2351352/1‑150 (MQ=255)
                                                                                                       aGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTAc                                             >  2:4171168/1‑150 (MQ=255)
                                                                                                        gTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACg                                            >  2:1810799/1‑150 (MQ=255)
                                                                                                           aTTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGc                                         >  1:2525192/1‑150 (MQ=255)
                                                                                                            ttACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCg                                        >  1:5310770/1‑150 (MQ=255)
                                                                                                               cAAATAAACCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGaca                                     <  2:2278485/150‑1 (MQ=255)
                                                                                                               cAAATAAAACGGGGTTCGTAATCATCAAGAAAACCCCACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGaca                                     <  1:310828/150‑1 (MQ=255)
                                                                                                               cAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGaca                                     >  2:2677611/1‑150 (MQ=255)
                                                                                                                aaaTAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaa                                    >  2:1209690/1‑150 (MQ=255)
                                                                                                                   tAAAACCGGGTTCGTAATAATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACAt                                 <  1:1838595/150‑1 (MQ=255)
                                                                                                                    aaaaCCGGGTTCGTAAACATAAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATc                                <  2:1886532/150‑1 (MQ=255)
                                                                                                                     aaaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCg                               >  2:4412659/1‑150 (MQ=255)
                                                                                                                     aaaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCg                               <  2:5024826/150‑1 (MQ=255)
                                                                                                                       aCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCt                             >  1:4215133/1‑150 (MQ=255)
                                                                                                                       aCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCt                             >  2:1994616/1‑150 (MQ=255)
                                                                                                                       aCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCt                             >  1:4401586/1‑150 (MQ=255)
                                                                                                                        gggggTTCGTAATCATCAAGAAACCCACCCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTa                            <  1:2970065/148‑1 (MQ=255)
                                                                                                                        ccGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTa                            <  2:2259682/150‑1 (MQ=255)
                                                                                                                         cGGGTTCGTAATCATCAATAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTaa                           <  2:5337708/150‑1 (MQ=255)
                                                                                                                             ttCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCa                       >  1:5077480/1‑150 (MQ=255)
                                                                                                                              tCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCag                      >  2:1614491/1‑150 (MQ=255)
                                                                                                                               cGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCaga                     >  1:3235588/1‑150 (MQ=255)
                                                                                                                               cGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCaga                     >  1:1660263/1‑150 (MQ=255)
                                                                                                                                 tAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAgaga                   >  1:3188529/1‑150 (MQ=255)
                                                                                                                                 tAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAgaga                   >  1:504087/1‑150 (MQ=255)
                                                                                                                                   atcatcAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACg                 >  1:4746301/1‑150 (MQ=255)
                                                                                                                                     aataaataaaacaaaacGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGt               <  2:3844956/143‑1 (MQ=255)
                                                                                                                                      atcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGtc              >  2:2979534/1‑149 (MQ=255)
                                                                                                                                      atcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTa              >  2:5090964/1‑150 (MQ=255)
                                                                                                                                      atcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTa              >  2:429789/1‑150 (MQ=255)
                                                                                                                                        caAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt            >  2:17596/1‑150 (MQ=255)
                                                                                                                                        caAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt            >  1:1079162/1‑150 (MQ=255)
                                                                                                                                         aaGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt           >  1:2930033/1‑150 (MQ=255)
                                                                                                                                         aaGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt           >  1:5520298/1‑150 (MQ=255)
                                                                                                                                         aaGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt           <  2:1011203/150‑1 (MQ=255)
                                                                                                                                         aaGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt           <  2:3078847/150‑1 (MQ=255)
                                                                                                                                          aGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTg          >  2:4116088/1‑150 (MQ=255)
                                                                                                                                           gAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGt         >  2:145188/1‑150 (MQ=255)
                                                                                                                                              aaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCt      >  2:4906993/1‑150 (MQ=255)
                                                                                                                                               aCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtc     >  1:4550348/1‑150 (MQ=255)
                                                                                                                                               aCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtc     <  2:689616/150‑1 (MQ=255)
                                                                                                                                                ccAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtca    >  1:2386364/1‑150 (MQ=255)
                                                                                                                                                 cAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcat   >  1:925396/1‑150 (MQ=255)
                                                                                                                                                  aaaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc  >  1:752670/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATC  >  CP009273/1784576‑1784871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑