Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,795,380 A→T 19.2% V483E (GTA→GAA)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,795,3800AT19.2% 784.1 / 174.7 264V483E (GTA→GAA) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (82/131);  new base T (25/26);  total (107/157)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

AAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCAC  >  CP009273/1795233‑1795529
                                                                                                                                                   |                                                                                                                                                     
aaaCCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtact                                                                                                                                                     <  2:4141987/150‑1 (MQ=255)
aaaCCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtact                                                                                                                                                     <  1:3729928/150‑1 (MQ=255)
   ccAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtactgta                                                                                                                                                  >  1:2423535/1‑150 (MQ=255)
        gAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCa                                                                                                                                             >  1:4669756/1‑150 (MQ=255)
         aaCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCAc                                                                                                                                            >  1:2603437/1‑150 (MQ=255)
          aCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACt                                                                                                                                           <  1:1004851/150‑1 (MQ=255)
                 ggTCAGGATGCCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                                                                                                                                    >  2:306167/1‑150 (MQ=255)
                  gTCCGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCAGTACCGGTACTTTACGTGCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCCAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCa                                                                                                                                   >  2:2856260/1‑150 (MQ=255)
                    cAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACg                                                                                                                                 <  1:3351052/150‑1 (MQ=255)
                    cAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACg                                                                                                                                 <  2:482278/150‑1 (MQ=255)
                      ggATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGAt                                                                                                                               >  1:3960835/1‑150 (MQ=255)
                       gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATc                                                                                                                              <  1:1688601/150‑1 (MQ=255)
                       gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATc                                                                                                                              <  1:1238865/150‑1 (MQ=255)
                        aTACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCg                                                                                                                             >  1:2332293/1‑150 (MQ=255)
                         tACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGa                                                                                                                            <  2:4321506/150‑1 (MQ=255)
                         tACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGa                                                                                                                            <  1:3266280/150‑1 (MQ=255)
                          aCCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAg                                                                                                                           >  2:3311895/1‑150 (MQ=255)
                             gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCa                                                                                                                        <  1:3042990/150‑1 (MQ=255)
                             gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCa                                                                                                                        <  2:4587165/150‑1 (MQ=255)
                              aTGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAg                                                                                                                       >  2:2781722/1‑150 (MQ=255)
                              aTGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAg                                                                                                                       <  1:2704887/150‑1 (MQ=255)
                                gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTc                                                                                                                     <  1:589612/150‑1 (MQ=255)
                                 aaaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCa                                                                                                                    >  1:4244905/1‑150 (MQ=255)
                                 aaaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCa                                                                                                                    >  2:1574186/1‑150 (MQ=255)
                                   aCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATa                                                                                                                  <  2:2631751/150‑1 (MQ=255)
                                   aCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATa                                                                                                                  <  1:1971351/150‑1 (MQ=255)
                                    cGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATAc                                                                                                                 <  2:5388465/150‑1 (MQ=255)
                                        ccATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCACAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAggg                                                                                                             >  2:779597/1‑150 (MQ=255)
                                         cATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGt                                                                                                            <  2:4597501/150‑1 (MQ=255)
                                          aTCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTa                                                                                                           >  2:2306807/1‑150 (MQ=255)
                                             gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAt                                                                                                        <  1:2591228/150‑1 (MQ=255)
                                             gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAt                                                                                                        <  2:2505252/150‑1 (MQ=255)
                                                      aTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTc                                                                                               <  2:1733497/150‑1 (MQ=255)
                                                      aTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTc                                                                                               >  2:1061824/1‑150 (MQ=255)
                                                          cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGTTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGAc                                                                                           <  1:3210161/150‑1 (MQ=255)
                                                          cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGAc                                                                                           <  2:2541509/150‑1 (MQ=255)
                                                             ggTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGt                                                                                        >  1:1818488/1‑150 (MQ=255)
                                                                 aaTCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGaa                                                                                    >  2:1213664/1‑150 (MQ=255)
                                                                       tACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                              >  1:5300183/1‑150 (MQ=255)
                                                                       tACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                              <  1:151245/150‑1 (MQ=255)
                                                                         ccGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCtt                                                                            <  1:1557474/150‑1 (MQ=255)
                                                                          cGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTc                                                                           >  2:4399671/1‑150 (MQ=255)
                                                                          cGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTc                                                                           <  1:5202538/150‑1 (MQ=255)
                                                                            gTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAc                                                                         >  2:2628954/1‑150 (MQ=255)
                                                                             tACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAcc                                                                        >  2:4971378/1‑150 (MQ=255)
                                                                                tttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                     <  1:1052576/150‑1 (MQ=255)
                                                                                 ttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGt                                                                    <  1:4840596/150‑1 (MQ=255)
                                                                                  tACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGtt                                                                   <  2:1347078/150‑1 (MQ=255)
                                                                                  tACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGtt                                                                   >  1:3663275/1‑150 (MQ=255)
                                                                                      ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGata                                                               <  1:5161599/150‑1 (MQ=255)
                                                                                       tCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGatat                                                              <  1:2119837/150‑1 (MQ=255)
                                                                                       tCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGACCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCTCCTTCACCCAGTTGatat                                                              >  1:2408094/1‑150 (MQ=255)
                                                                                        cATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATAtt                                                             <  1:611148/150‑1 (MQ=255)
                                                                                        cATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATAtt                                                             <  1:4283749/150‑1 (MQ=255)
                                                                                        cATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATAtt                                                             <  1:978886/150‑1 (MQ=255)
                                                                                         aTTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTc                                                            <  2:2710344/150‑1 (MQ=255)
                                                                                         aTTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTc                                                            >  1:5496409/1‑150 (MQ=255)
                                                                                          ttGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCa                                                           <  2:44472/150‑1 (MQ=255)
                                                                                           tGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCaa                                                          <  1:4852871/150‑1 (MQ=255)
                                                                                                     tACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgtt                                                <  1:5277293/150‑1 (MQ=255)
                                                                                                       cATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                     <  2:598262/127‑1 (MQ=255)
                                                                                                       cATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgttgt                                              <  1:3996825/150‑1 (MQ=255)
                                                                                                        aTAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATgttgtt                                             <  1:1907611/150‑1 (MQ=255)
                                                                                                         tAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGttt                                            <  1:3186732/150‑1 (MQ=255)
                                                                                                           agaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttc                                          <  1:4418780/150‑1 (MQ=255)
                                                                                                           agaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttc                                          >  1:2358114/1‑150 (MQ=255)
                                                                                                            gaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttct                                         <  2:4901081/150‑1 (MQ=255)
                                                                                                               gcgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTcc                                      <  1:413213/150‑1 (MQ=255)
                                                                                                                cgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCa                                     <  1:4509801/150‑1 (MQ=255)
                                                                                                                cgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCa                                     >  2:1450518/1‑150 (MQ=255)
                                                                                                                 gcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAg                                    >  1:1174788/1‑150 (MQ=255)
                                                                                                                  cTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAgc                                   <  1:2815880/150‑1 (MQ=255)
                                                                                                                     agacgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCa                                <  1:3384476/150‑1 (MQ=255)
                                                                                                                       acgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCacc                              >  2:3527306/1‑148 (MQ=255)
                                                                                                                        cgagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAAcc                             <  1:3135783/150‑1 (MQ=255)
                                                                                                                         gagacgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCg                            >  1:3339461/1‑150 (MQ=255)
                                                                                                                          agacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGc                           >  1:827030/1‑150 (MQ=255)
                                                                                                                            acgGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCa                         <  1:3017523/150‑1 (MQ=255)
                                                                                                                              ggCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAgg                       >  2:362898/1‑150 (MQ=255)
                                                                                                                                cAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCACAACCGCCAGGTc                     <  2:1849845/150‑1 (MQ=255)
                                                                                                                                 aaaGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTcc                    <  1:2868139/150‑1 (MQ=255)
                                                                                                                                  aaGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCg                   >  1:127924/1‑150 (MQ=255)
                                                                                                                                    gagaAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGcc                 >  2:3396304/1‑150 (MQ=255)
                                                                                                                                    gagaAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGcc                 >  2:5330941/1‑150 (MQ=255)
                                                                                                                                      gaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTc               <  1:1784003/150‑1 (MQ=255)
                                                                                                                                      gaAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTc               <  2:4850596/150‑1 (MQ=255)
                                                                                                                                       aaGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCa              >  1:5100829/1‑150 (MQ=255)
                                                                                                                                        aGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAg             >  1:5013542/1‑150 (MQ=255)
                                                                                                                                           ccAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTCGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCAc          >  2:5034682/1‑150 (MQ=255)
                                                                                                                                            cAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACg         >  2:2088804/1‑150 (MQ=255)
                                                                                                                                            cAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACg         <  2:4733260/150‑1 (MQ=255)
                                                                                                                                            cAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACg         <  2:4774210/150‑1 (MQ=255)
                                                                                                                                              gCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGAt       <  2:4285076/150‑1 (MQ=255)
                                                                                                                                              gCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGAt       <  2:4886453/150‑1 (MQ=255)
                                                                                                                                               ctgtactgtaCCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATc      <  2:3960835/150‑1 (MQ=255)
                                                                                                                                                tgtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATcc     <  2:5298755/150‑1 (MQ=255)
                                                                                                                                                 gtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATccc    >  1:4045469/1‑150 (MQ=255)
                                                                                                                                                 gtactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATccc    <  2:2962194/150‑1 (MQ=255)
                                                                                                                                                  tactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCca   <  1:4967176/150‑1 (MQ=255)
                                                                                                                                                   actgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCcac  >  2:1185036/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCCAC  >  CP009273/1795233‑1795529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑