Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,428 C→A 12.8% V134F (GTC→TTC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,4280CA12.8% 1019.8 / 118.1 297V134F (GTC→TTC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (125/134);  new base A (17/21);  total (142/155)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 306 total aligned reads displayed.

GCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTT  >  CP009273/1796279‑1796577
                                                                                                                                                     |                                                                                                                                                     
 cgGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAcg                                                                                                                                                      >  1:4868020/1‑150 (MQ=255)
      caTATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTa                                                                                                                                                 <  1:4837473/150‑1 (MQ=255)
      caTATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTa                                                                                                                                                 <  2:2228365/150‑1 (MQ=255)
       atatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAg                                                                                                                                                <  1:4899867/150‑1 (MQ=255)
        tatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGt                                                                                                                                               <  2:564764/150‑1 (MQ=255)
        tatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGt                                                                                                                                               >  1:3087688/1‑150 (MQ=255)
        tatCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGt                                                                                                                                               >  1:3709118/1‑150 (MQ=255)
           cGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGtttt                                                                                                                                            <  1:3182998/150‑1 (MQ=255)
           cGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGtttt                                                                                                                                            <  1:1678682/150‑1 (MQ=255)
            gACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGttttt                                                                                                                                           <  1:4478496/150‑1 (MQ=255)
               atatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTtatc                                                                                                                                        >  1:3905973/1‑150 (MQ=255)
                tatTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCa                                                                                                                                       >  1:500029/1‑150 (MQ=255)
                  ttcttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGc                                                                                                                                     <  1:1120672/150‑1 (MQ=255)
                     ttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAg                                                                                                                                  <  2:508610/150‑1 (MQ=255)
                     ttcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGAAAg                                                                                                                                  >  1:2793555/1‑150 (MQ=255)
                      tcATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGc                                                                                                                                 >  1:73047/1‑150 (MQ=255)
                       cATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCt                                                                                                                                >  1:1736312/1‑150 (MQ=255)
                       cATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCt                                                                                                                                >  2:4026326/1‑150 (MQ=255)
                         tGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCa                                                                                                                              <  2:3964589/150‑1 (MQ=255)
                          ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCAt                                                                                                                             >  1:2260189/1‑150 (MQ=255)
                          ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCAt                                                                                                                             <  2:1107126/150‑1 (MQ=255)
                          ggAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCAt                                                                                                                             <  1:627190/150‑1 (MQ=255)
                            aaGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGc                                                                                                                           <  1:3123242/150‑1 (MQ=255)
                             aGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCa                                                                                                                          >  2:5153071/1‑150 (MQ=255)
                               tACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATc                                                                                                                        >  2:2925014/1‑150 (MQ=255)
                                  aGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc                                                                                                                     >  2:719522/1‑150 (MQ=255)
                                  aGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGc                                                                                                                     >  1:1071817/1‑150 (MQ=255)
                                      cTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtct                                                                                                                 <  2:1806047/150‑1 (MQ=255)
                                       tGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtctc                                                                                                                >  1:2713720/1‑150 (MQ=255)
                                        ggCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCg                                                                                                               <  2:3551621/150‑1 (MQ=255)
                                         gCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGa                                                                                                              >  1:155945/1‑150 (MQ=255)
                                           ttGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGt                                                                                                            <  2:2521618/150‑1 (MQ=255)
                                                    tGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACa                                                                                                   <  1:2028561/150‑1 (MQ=255)
                                                       gCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCt                                                                                                <  2:3725624/150‑1 (MQ=255)
                                                        cGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCtt                                                                                               >  1:5200758/1‑150 (MQ=255)
                                                         gATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCACGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTc                                                                                              >  1:1042184/1‑150 (MQ=255)
                                                           tGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCt                                                                                            <  1:877155/150‑1 (MQ=255)
                                                             ttttCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTgg                                                                                          <  1:2838384/150‑1 (MQ=255)
                                                              tttCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAAGGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTggg                                                                                         <  2:4905083/150‑1 (MQ=255)
                                                                 cGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTa                                                                                      >  2:2086041/1‑150 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                    <  1:4793675/150‑1 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                    <  1:3831262/150‑1 (MQ=255)
                                                                   tCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAAc                                                                                    >  1:1179274/1‑150 (MQ=255)
                                                                    cAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACg                                                                                   <  2:4814160/150‑1 (MQ=255)
                                                                    cAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACg                                                                                   <  2:5534952/150‑1 (MQ=255)
                                                                      aGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTg                                                                                 >  1:2613398/1‑150 (MQ=255)
                                                                      aGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTg                                                                                 >  1:1804355/1‑150 (MQ=255)
                                                                      aGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTg                                                                                 <  1:616094/150‑1 (MQ=255)
                                                                          tGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGt                                                                             <  1:4690027/150‑1 (MQ=255)
                                                                            gagaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCa                                                                           >  1:132159/1‑150 (MQ=255)
                                                                             agaCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCaa                                                                          <  2:151867/150‑1 (MQ=255)
                                                                                cTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGAt                                                                       >  2:4548607/1‑150 (MQ=255)
                                                                                   tGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCaa                                                                    <  2:1325590/150‑1 (MQ=255)
                                                                                    gTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAc                                                                   >  1:3535099/1‑150 (MQ=255)
                                                                                    gTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAc                                                                   >  2:1310521/1‑150 (MQ=255)
                                                                                     tAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcg                                                                  >  1:2183598/1‑150 (MQ=255)
                                                                                       gCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtc                                                                <  2:3032668/150‑1 (MQ=255)
                                                                                        ctctCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgtcg                                                               >  1:545971/1‑150 (MQ=255)
                                                                                           tcCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGtaa                                                            >  1:4219730/1‑150 (MQ=255)
                                                                                               cACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaa                                                        >  2:1957038/1‑150 (MQ=255)
                                                                                                 cGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaaaa                                                      <  2:1447209/150‑1 (MQ=255)
                                                                                                 cGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATaaaaa                                                      >  1:2653650/1‑150 (MQ=255)
                                                                                                  ggTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAc                                                     >  2:2058338/1‑150 (MQ=255)
                                                                                                  ggTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAc                                                     >  2:2934590/1‑150 (MQ=255)
                                                                                                  ggTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAc                                                     >  1:1584904/1‑150 (MQ=255)
                                                                                                   gTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAAcc                                                    <  2:798481/150‑1 (MQ=255)
                                                                                                         gAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTc                                              >  2:5217768/1‑150 (MQ=255)
                                                                                                          aaaGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCa                                             <  2:4845197/150‑1 (MQ=255)
                                                                                                              tttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTTATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTAGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGATGTCAATat                                         <  2:2760941/150‑2 (MQ=255)
                                                                                                               ttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAAc                                        <  1:3233821/150‑1 (MQ=255)
                                                                                                               ttCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAAc                                        <  1:2947993/150‑1 (MQ=255)
                                                                                                                     cgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGcc                                  <  1:5153071/150‑1 (MQ=255)
                                                                                                                     cgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGcc                                  <  2:3179827/150‑1 (MQ=255)
                                                                                                                     cgcTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGcc                                  <  1:4256054/150‑1 (MQ=255)
                                                                                                                       cTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGa                                <  2:4353098/150‑1 (MQ=255)
                                                                                                                        ttCGTGCCAGCTGACTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGAt                               >  2:3615900/1‑150 (MQ=255)
                                                                                                                          cGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTg                             >  2:3360640/1‑150 (MQ=255)
                                                                                                                            tGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGcc                           >  2:321413/1‑150 (MQ=255)
                                                                                                                            tGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATACGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCTGGCCGATTGcc                           <  1:98361/150‑1 (MQ=255)
                                                                                                                              ccAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAt                         >  2:4148814/1‑150 (MQ=255)
                                                                                                                              ccAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAt                         >  2:4066677/1‑150 (MQ=255)
                                                                                                                                aGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAttt                       >  1:4327734/1‑150 (MQ=255)
                                                                                                                                aGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAttt                       >  1:2873001/1‑150 (MQ=255)
                                                                                                                                  cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                     <  2:477855/150‑1 (MQ=255)
                                                                                                                                  cTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                     <  1:4763188/150‑1 (MQ=255)
                                                                                                                                   tGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTgg                    <  1:1768816/150‑1 (MQ=255)
                                                                                                                                   tGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTgg                    <  2:4766417/150‑1 (MQ=255)
                                                                                                                                   tGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTACGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTgg                    <  2:2672019/150‑1 (MQ=255)
                                                                                                                                    gACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGt                   <  1:3490642/150‑1 (MQ=255)
                                                                                                                                      cTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTAt                 <  2:3112160/150‑1 (MQ=255)
                                                                                                                                      cTTTCTTCTTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTAt                 <  1:2537672/150‑1 (MQ=255)
                                                                                                                                        ttcttcTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGc               >  1:846365/1‑150 (MQ=255)
                                                                                                                                        ttcttcTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCAATTTGGTATGc               >  1:5283107/1‑150 (MQ=255)
                                                                                                                                         tcttctTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCg              <  2:5070032/150‑1 (MQ=255)
                                                                                                                                         tcttctTAATGAAGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATATTCCAGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCg              >  1:1052016/1‑150 (MQ=255)
                                                                                                                                           ttcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGc            >  1:4094162/1‑150 (MQ=255)
                                                                                                                                            tcttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGcc           >  2:3850027/1‑150 (MQ=255)
                                                                                                                                             cttAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCa          <  1:1494019/150‑1 (MQ=255)
                                                                                                                                                aaTGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAg       >  2:912048/1‑150 (MQ=255)
                                                                                                                                                    aCGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgttgt   <  1:2433289/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTT  >  CP009273/1796279‑1796577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑