Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,843 A→C 5.6% F50C (TTT→TGT)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8430AC 5.6% 896.0 / 3.9 289F50C (TTT→TGT) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (177/87);  new base C (3/22);  total (180/109)
Fisher's exact test for biased strand distribution p-value = 8.42e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

GTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCAT  >  CP009273/1823696‑1823991
                                                                                                                                                    |                                                                                                                                                    
gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                                    <  1:1193634/150‑1 (MQ=255)
gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                                    >  2:3913627/1‑150 (MQ=255)
 tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcg                                                                                                                                                   >  2:4888502/1‑150 (MQ=255)
 tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcg                                                                                                                                                   >  2:4272409/1‑150 (MQ=255)
 tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcg                                                                                                                                                   >  2:4041505/1‑150 (MQ=255)
 tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcg                                                                                                                                                   >  2:1275265/1‑150 (MQ=255)
   aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                                                                 >  2:912406/1‑150 (MQ=255)
   aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                                                                 >  1:3912879/1‑150 (MQ=255)
   aCTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgcc                                                                                                                                                 >  1:2652737/1‑150 (MQ=255)
    cTTGGGTTGCCGCTTCCCTGCGCGTCTTACCCGTTTCTCTGGAAATAATCGCGTTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCTTTCGACAACGGAATTACGTTCAGAAAATTAAAGCCGCGCCCAGCGCGGAAAcgccg                                                                                                                                                <  2:5329462/150‑1 (MQ=255)
      tCGTTTGCCGCTTCCCTGGGGGTTTTACCCTTTTATGTGGAAATATTCGCGTTTATTTCGGCTTTTTTGATTTCATGAATTGCGGCAAA‑GGGTTCGAAAACGGATTGACTTTAATAAATTTAAAGCCGCGCCCAGCGCGGAAACGCCGCa                                                                                                                                              <  2:1295771/150‑1 (MQ=255)
        ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                                                            >  1:4802758/1‑150 (MQ=255)
        ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                                                            >  1:3604833/1‑150 (MQ=255)
        ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACg                                                                                                                                            >  1:3519029/1‑150 (MQ=255)
         gTTGCCGCTTCCCCGCGGGGCTTACCCTTTTCTCTGGCAATATTCGGGGTTATTTCGGCTTTTTGGCTTTCCTGCAGTGCGGCAAA‑GCTTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACgg                                                                                                                                           <  2:3311283/150‑1 (MQ=255)
           tGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGc                                                                                                                                         >  2:1738737/1‑150 (MQ=255)
           tGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGc                                                                                                                                         >  1:4412691/1‑150 (MQ=255)
                gttCCCTGCGGGGCTTACCCGTTTCTCTGGCAATAATCGCGTTTAATTCGGCTTTATTGCTTTCCTGCAGTGCGGATAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGccc                                                                                                                                    <  2:433615/149‑1 (MQ=255)
                cTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGccc                                                                                                                                    <  2:1233245/150‑1 (MQ=255)
                 tgccccGCGCGGCTTACCCGTTTCTCGGGCAATAATCGGGGTTATTTCGGCTTTTTTGCTTTCCCGCCGGGCGGAAAC‑GCGTTCGACAACGGAATGAGGTTCAGCAAATTAAAGCCGCGCCCTGCGCGGACACGCCGCACGGGCTGCCCg                                                                                                                                   <  2:43049/148‑1 (MQ=255)
                  tCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGa                                                                                                                                  >  2:4452931/1‑150 (MQ=255)
                      aGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAg                                                                                                                              <  2:3396737/150‑1 (MQ=255)
                         gcgGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCt                                                                                                                           >  2:4576335/1‑150 (MQ=255)
                           ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                         >  1:4767996/1‑150 (MQ=255)
                           ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                         >  2:5098777/1‑150 (MQ=255)
                              ttACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCg                                                                                                                      >  2:2227470/1‑150 (MQ=255)
                                  ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                                  >  2:4639898/1‑150 (MQ=255)
                                        ctctGGCAATAATCGCGGTTAATTCGGCTTTTTTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGAATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcgg                                                                                                            <  2:4521095/150‑1 (MQ=255)
                                        ctctGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcgg                                                                                                            >  2:688188/1‑150 (MQ=255)
                                           tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                                                                         >  2:1656706/1‑150 (MQ=255)
                                            ggCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                        >  1:851972/1‑150 (MQ=255)
                                              cAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcgg                                                                                                      >  1:1597716/1‑150 (MQ=255)
                                                 taatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                                   >  1:4490503/1‑150 (MQ=255)
                                                 taatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                                   >  1:1447464/1‑150 (MQ=255)
                                                   atCGCGGTTATTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatca                                                                                                 <  2:1530915/150‑1 (MQ=255)
                                                     cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                               >  2:2775252/1‑150 (MQ=255)
                                                      gcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                                                                              >  1:2420598/1‑150 (MQ=255)
                                                             aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                                       <  2:2948365/150‑1 (MQ=255)
                                                             aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                                       >  1:4916472/1‑150 (MQ=255)
                                                             aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                                       >  1:3421963/1‑150 (MQ=255)
                                                              ttCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                                                                      >  1:5149818/1‑150 (MQ=255)
                                                              ttCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCa                                                                                      >  2:3790027/1‑150 (MQ=255)
                                                               tCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCAt                                                                                     >  2:2403408/1‑150 (MQ=255)
                                                                 ggCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                                                                   >  2:871630/1‑150 (MQ=255)
                                                                 ggCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                                                                   >  1:4415839/1‑150 (MQ=255)
                                                                  gtttttttGTTTTCCAGCTGTG‑GGCAAACGGTTTCGACACCGGCTTGAGGTCCAGAAAATCAAAGCCGCGCCCCGCGCGTACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                                  <  1:4293555/148‑1 (MQ=255)
                                                                     ttATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                                                               >  1:4214277/1‑150 (MQ=255)
                                                                      tATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                              >  2:641214/1‑150 (MQ=255)
                                                                      tATTGCTTACCAGCTGGGCGGAAAT‑GCGTTCGACACCGGATTGACGTTCCGCAAATGACCGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                              <  1:2555328/150‑1 (MQ=255)
                                                                       aTTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTc                                                                             >  2:1091679/1‑150 (MQ=255)
                                                                          gCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGcc                                                                          >  1:3827626/1‑150 (MQ=255)
                                                                               ccAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                                                                     >  2:2426902/1‑150 (MQ=255)
                                                                                   cagTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCgg                                                                 >  2:393401/1‑150 (MQ=255)
                                                                                    agTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa                                                                >  1:4684535/1‑150 (MQ=255)
                                                                                    agTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGa                                                                >  1:4684700/1‑150 (MQ=255)
                                                                                      tGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAtt                                                              >  2:5425701/1‑150 (MQ=255)
                                                                                      tGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAtt                                                              <  1:4064700/150‑1 (MQ=255)
                                                                                        cGGCAAA‑GCGTACGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAc                                                            <  2:1176083/150‑1 (MQ=255)
                                                                                         ggCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACg                                                           >  1:285199/1‑150 (MQ=255)
                                                                                         ggCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACg                                                           >  2:5028324/1‑150 (MQ=255)
                                                                                                 gtttCGACAACGGAATTACGTTCAGAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                                    <  1:5405962/148‑1 (MQ=255)
                                                                                                  tttCGAAAACGGCATGACTTTCAGCAAATCAAAGCCGCGCCCAGCGCGTACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCacac                                                   <  1:1611561/149‑1 (MQ=255)
                                                                                                   ttCGACAACGGATTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAcg                                                  <  1:5245925/150‑1 (MQ=255)
                                                                                                   tgcGACAACGGCATGACGTTCAGAAAATTAAAGCCGCGCCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAcg                                                  <  1:1970901/148‑1 (MQ=255)
                                                                                                       acacCGGAATGACTTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                              <  2:1996837/150‑1 (MQ=255)
                                                                                                       aCAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                              >  2:618066/1‑150 (MQ=255)
                                                                                                       aCAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                              <  2:4793553/150‑1 (MQ=255)
                                                                                                        cAACGGCATGACGTTCAGCAATTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACCCGCGATg                                             <  2:5519739/150‑1 (MQ=255)
                                                                                                            ggaaTGACGTTCACCAAATGAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGcc                                         <  1:5346313/147‑1 (MQ=255)
                                                                                                              cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCt                                       >  2:208538/1‑150 (MQ=255)
                                                                                                               aTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTg                                      >  2:3157957/1‑150 (MQ=255)
                                                                                                               aTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTg                                      >  1:3983746/1‑150 (MQ=255)
                                                                                                               aTGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTg                                      <  2:361628/150‑1 (MQ=255)
                                                                                                                tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTgg                                     <  2:2870687/150‑1 (MQ=255)
                                                                                                                tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTgg                                     >  2:1774761/1‑150 (MQ=255)
                                                                                                                   ctttCAGCAAATGAACGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGccc                                  <  1:148958/148‑1 (MQ=255)
                                                                                                                   cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGccc                                  <  1:1795398/150‑1 (MQ=255)
                                                                                                                   cGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGccc                                  >  1:1177117/1‑150 (MQ=255)
                                                                                                                    gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCg                                 >  2:3011296/1‑150 (MQ=255)
                                                                                                                       cAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                              >  2:1690612/1‑150 (MQ=255)
                                                                                                                        aGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTAt                             >  2:3055410/1‑150 (MQ=255)
                                                                                                                         gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATc                            <  2:3089136/150‑1 (MQ=255)
                                                                                                                          cAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATcc                           >  1:1685028/1‑150 (MQ=255)
                                                                                                                          aaatcgAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATcc                           <  1:5288203/145‑1 (MQ=255)
                                                                                                                           aaaTGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                          <  1:2896746/150‑1 (MQ=255)
                                                                                                                           aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                          <  2:2550253/150‑1 (MQ=255)
                                                                                                                            aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                         <  2:1556620/150‑1 (MQ=255)
                                                                                                                            aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                         >  1:1362674/1‑150 (MQ=255)
                                                                                                                             aTGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                        <  2:5161314/150‑1 (MQ=255)
                                                                                                                              tGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc                       <  2:3329047/150‑1 (MQ=255)
                                                                                                                                aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTACACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc                     >  2:1532997/1‑150 (MQ=255)
                                                                                                                                 aaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAcc                    <  1:309532/150‑1 (MQ=255)
                                                                                                                                     cgcgCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTa                <  1:871630/150‑1 (MQ=255)
                                                                                                                                     cgcgCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTa                <  1:3637765/150‑1 (MQ=255)
                                                                                                                                        gcCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATc             <  2:4801369/150‑1 (MQ=255)
                                                                                                                                          ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCa           >  1:4562407/1‑150 (MQ=255)
                                                                                                                                           cAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt          >  1:596067/1‑150 (MQ=255)
                                                                                                                                           cAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt          <  2:1429669/150‑1 (MQ=255)
                                                                                                                                           cAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt          >  1:2513168/1‑150 (MQ=255)
                                                                                                                                                 ggAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTc    >  2:530934/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAA‑GCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCAT  >  CP009273/1823696‑1823991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑