Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,673 A→C 6.3% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,6730AC 6.3% 882.5 / 5.0 283V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (188/68);  new base C (2/25);  total (190/93)
Fisher's exact test for biased strand distribution p-value = 1.38e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA  >  CP009273/2711525‑2711821
                                                                                                                                                     |                                                                                                                                                    
   gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                  >  1:3722148/1‑150 (MQ=255)
   gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                  <  2:47622/150‑1 (MQ=255)
    aaaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTg                                                                                                                                                 >  2:3346213/1‑150 (MQ=255)
          gCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc                                                                                                                                           >  1:3626825/1‑150 (MQ=255)
          gCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc                                                                                                                                           >  2:4807525/1‑150 (MQ=255)
           ccTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACg                                                                                                                                          >  2:329102/1‑150 (MQ=255)
           ccTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACg                                                                                                                                          <  1:3220918/150‑1 (MQ=255)
            cTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACgg                                                                                                                                         >  2:3012187/1‑150 (MQ=255)
            cTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACgg                                                                                                                                         >  2:4074769/1‑150 (MQ=255)
             tGACATGCATTGTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACAATTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCATTTTCGGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGc                                                                                                                                        <  2:4398427/150‑1 (MQ=255)
                 atgatttttCGCCTTAGACGGGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGTATAAATAAAACTTTTACCAATTATGCCACG‑GTGTTCGGCTTTTTCCGGGGTCTAATCACCCGGCGGGGGGTAAACGGTGCGCCACGGCGAAt                                                                                                                                    <  2:3525140/145‑1 (MQ=255)
                    cATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCtt                                                                                                                                 >  1:1704993/1‑150 (MQ=255)
                    cATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCtt                                                                                                                                 >  1:2052492/1‑150 (MQ=255)
                     aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                                                                                                                >  2:1750989/1‑150 (MQ=255)
                     aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                                                                                                                >  1:1363380/1‑150 (MQ=255)
                          cGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACt                                                                                                                           >  2:1276411/1‑150 (MQ=255)
                          cGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACt                                                                                                                           >  2:937476/1‑150 (MQ=255)
                                        gTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGc                                                                                                             >  1:1982478/1‑150 (MQ=255)
                                          ttcttcCGCACGCTGACGACGAAACACTGCCGGATCAATAAAACTTTTCCCACTGATGCCACG‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGGGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg                                                                                                           <  2:3980385/150‑1 (MQ=255)
                                          ttctccCGCACGGTCCCGCCGCAACCCTACCGGAACAAAAAAAATTTTCCCCCTCATCCCACC‑GTGAGCGGCGTGGCCGGCCTTCTCTTCACCCGGCGCGCGGTAACCCGTGCGCCACGGCGAATCTTCCCACGTGCGGTCACGGGGCTg                                                                                                           <  2:2696612/150‑1 (MQ=255)
                                            ctgccGCACGCTGACGACGCAACACTGCCGGAACAATAAAACTTTTCCCACTG‑TGCCCCCGGTGAGGGGCCTTTGCCGGCGTCTCAACCCCCGGCGCGGGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                         <  2:3877547/147‑1 (MQ=255)
                                              tcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActc                                                                                                       >  2:362748/1‑150 (MQ=255)
                                                    cgatcacgacgCAACACTACCGCATAAATAAAACTTTTCCCACTGAGCCCACC‑GTGATCGGCATTTCCGGGCGTCACAACACCCGGCGCGCGGCAAACCGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg                                                                                                 <  2:1289156/145‑1 (MQ=255)
                                                        gacgacGCACCACTTCCGGATCAATAAAACTTTTACCACGGATGCCACC‑GTTATCGGCCTTTCCCGGCGTCACACCACCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCAt                                                                                             <  2:1770594/150‑1 (MQ=255)
                                                         acgacgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATc                                                                                            >  1:1038559/1‑150 (MQ=255)
                                                          cgacgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcg                                                                                           >  2:3396297/1‑150 (MQ=255)
                                                             cgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgg                                                                                        >  2:4297227/1‑150 (MQ=255)
                                                             ccaaaCACTCCCGGATCAATAAAACTTTTACCACTTATGCCACC‑GTGATCGGCATTTCCCGGCGTCTAATCACCCGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgg                                                                                        <  2:4498626/147‑1 (MQ=255)
                                                              gCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcggg                                                                                       >  1:119701/1‑150 (MQ=255)
                                                              gCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcggg                                                                                       >  2:2328987/1‑150 (MQ=255)
                                                              gCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGGTTGTCATCgcggg                                                                                       >  1:3488871/1‑150 (MQ=255)
                                                                aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                     >  2:958114/1‑150 (MQ=255)
                                                                aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                     >  2:2113431/1‑150 (MQ=255)
                                                                  cacTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg                                                                                   >  2:2880497/1‑150 (MQ=255)
                                                                      tCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGa                                                                               >  2:2928296/1‑150 (MQ=255)
                                                                       ccGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGAc                                                                              >  1:5386600/1‑150 (MQ=255)
                                                                         ggATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                            >  1:2531966/1‑150 (MQ=255)
                                                                          gATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGc                                                                           >  2:1145347/1‑150 (MQ=255)
                                                                           aacAATAAACCTTTTACCACTGATGCCACC‑GTGATCGGCATTTGCCGGCGTCTCAACCCCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGcc                                                                          <  2:1254594/148‑1 (MQ=255)
                                                                           aTCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGcc                                                                          >  2:256699/1‑150 (MQ=255)
                                                                            tCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCa                                                                         >  1:1112469/1‑150 (MQ=255)
                                                                             cAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  2:1891964/1‑150 (MQ=255)
                                                                             cAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  2:865735/1‑150 (MQ=255)
                                                                             cAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                        >  1:2684749/1‑150 (MQ=255)
                                                                              aaTAAAACTTTAACCACTGATCCCACG‑GTTATCGGCTTTTTCCGGCGTCACATCACCGGGCGCGGGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                       <  1:4976559/150‑1 (MQ=255)
                                                                               aTAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                      >  1:323810/1‑150 (MQ=255)
                                                                                 aaaaCTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGACTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTc                                                                    >  1:551671/1‑150 (MQ=255)
                                                                                        ttACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACga                                                             >  2:3691331/1‑150 (MQ=255)
                                                                                         tACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGACACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgag                                                            >  1:1006096/1‑150 (MQ=255)
                                                                                         tACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgag                                                            >  1:1006307/1‑150 (MQ=255)
                                                                                         tACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgag                                                            >  1:3984322/1‑150 (MQ=255)
                                                                                           ccACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAt                                                          >  2:5147977/1‑150 (MQ=255)
                                                                                            cACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                                                         >  1:1124774/1‑150 (MQ=255)
                                                                                             aCTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAttt                                                        >  1:533719/1‑150 (MQ=255)
                                                                                              cggATGCCACC‑GTGAGCGGCCTTTTCCGGCGTCCCATCACCCGGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                       <  2:3656870/148‑1 (MQ=255)
                                                                                              cTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                       >  2:275378/1‑150 (MQ=255)
                                                                                               tGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                      >  2:1014540/1‑150 (MQ=255)
                                                                                                 aTGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                    >  1:1896684/1‑150 (MQ=255)
                                                                                                 aTGCCACC‑GTGATCGGCCTTTTCCGGCGTATCATCACCGGGCGCGGGGCAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                    <  2:5057508/150‑1 (MQ=255)
                                                                                                  tGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTg                                                   >  1:817556/1‑150 (MQ=255)
                                                                                                      cacgtggATCGGCATTTTCCGGGTTCTCATCCCCGGGCGCGGGGGAAACGGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAc                                                <  2:494283/144‑1 (MQ=255)
                                                                                                          gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                            >  1:240536/1‑150 (MQ=255)
                                                                                                           tGATCGGCCTTTACGGGCGTATCACCACCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg                                           <  1:900238/150‑1 (MQ=255)
                                                                                                            gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                          >  2:661778/1‑150 (MQ=255)
                                                                                                                ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                      >  1:1081967/1‑150 (MQ=255)
                                                                                                                ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                      >  2:948227/1‑150 (MQ=255)
                                                                                                                  ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                    >  2:2092253/1‑150 (MQ=255)
                                                                                                                     ttcccGGCTTCTCAACCCCCGGCGCGCGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTcc                                 <  1:3588592/147‑1 (MQ=255)
                                                                                                                      ttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGTCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                >  1:4432105/1‑150 (MQ=255)
                                                                                                                      ttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGTCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                >  1:2914830/1‑150 (MQ=255)
                                                                                                                          ggCGTCTCATCACCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                            <  2:2657992/150‑1 (MQ=255)
                                                                                                                             gTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                         >  2:5397650/1‑150 (MQ=255)
                                                                                                                              tcacatccCCCGGGGGGCGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgcg                        <  1:4879171/147‑1 (MQ=255)
                                                                                                                               ctcATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCgg                       >  2:1861304/1‑150 (MQ=255)
                                                                                                                                 catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                     >  1:304131/1‑150 (MQ=255)
                                                                                                                                 catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                     >  1:3673522/1‑150 (MQ=255)
                                                                                                                                 catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                     >  2:2847202/1‑150 (MQ=255)
                                                                                                                                 catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                     >  1:546616/1‑150 (MQ=255)
                                                                                                                                  accaCCCGGCGCGCGGGAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                    <  2:2532829/148‑1 (MQ=255)
                                                                                                                                   tccccgggCGCGGGGGACCCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                   <  2:462259/144‑1 (MQ=255)
                                                                                                                                   tcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                   >  1:4559745/1‑150 (MQ=255)
                                                                                                                                   tcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                   >  1:1798246/1‑150 (MQ=255)
                                                                                                                                   cccccgggCGCGGGGCAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                   <  1:4622329/144‑1 (MQ=255)
                                                                                                                                    cccccGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                  <  2:518390/148‑1 (MQ=255)
                                                                                                                                    caCCCGGCGCGGGGCAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                  <  2:1623884/150‑1 (MQ=255)
                                                                                                                                    caCCCGGCGCGCGGTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                  <  2:774546/150‑1 (MQ=255)
                                                                                                                                    caCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                  >  1:850935/1‑150 (MQ=255)
                                                                                                                                          gcgcgcGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTAc            <  1:4054982/150‑1 (MQ=255)
                                                                                                                                           cgcgcgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACg           <  2:344643/150‑1 (MQ=255)
                                                                                                                                           cgcgcgGGAAACCGGGCGCCACGGCGAATCTTCCCACGTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACg           <  2:139294/150‑1 (MQ=255)
                                                                                                                                           cgcgcgGCAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACg           <  1:2164389/150‑1 (MQ=255)
                                                                                                                                               cgGGACCCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa       <  2:203249/150‑1 (MQ=255)
                                                                                                                                               cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa       >  2:925818/1‑150 (MQ=255)
                                                                                                                                               cgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa       >  1:5164907/1‑150 (MQ=255)
                                                                                                                                                gggAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt      <  2:2290177/150‑1 (MQ=255)
                                                                                                                                                  gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa    >  2:4893798/1‑150 (MQ=255)
                                                                                                                                                  gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa    >  2:1735786/1‑150 (MQ=255)
                                                                                                                                                   aaaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg   >  2:59005/1‑150 (MQ=255)
                                                                                                                                                   aaaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg   >  1:3542574/1‑150 (MQ=255)
                                                                                                                                                    aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATGTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTCCGTTTATCAGa  >  1:3170759/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACC‑GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA  >  CP009273/2711525‑2711821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑