BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,385,493	G→T	8.2%	intergenic (‑106/+324)	tldD ← / ← yhdP	putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,385,493	0	G	T	8.2%	971.2 / 3.6	342	intergenic (‑106/+324)	tldD/yhdP	putative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand): ref base G (184/105); new base T (5/45); total (191/151)
Fisher's exact test for biased strand distribution p-value = 3.85e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 345 total aligned reads displayed.

CCGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTT  >  CP009273/3385344‑3385640

 cGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCATCCTACGGTTATGTTCCGGATTGTAGGCCTGa                                                                                                                                                    >  1:433173/1‑150 (MQ=255)

  gTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGAt                                                                                                                                                   <  2:2482577/150‑1 (MQ=255)

   ttCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATa                                                                                                                                                  >  2:1191981/1‑150 (MQ=255)

    tCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATaa                                                                                                                                                 <  1:3455614/150‑1 (MQ=255)

       ccgcTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGAc                                                                                                                                              <  2:5243526/150‑1 (MQ=255)

          cTAGCAATTGTTCACTTACCAGGTTAAGACTTATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGGTATGTTCCGGTTTGTAGGCCTGATAAGACGca                                                                                                                                           >  1:2322276/1‑150 (MQ=255)

          cTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACTca                                                                                                                                           >  1:486485/1‑150 (MQ=255)

          cTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGca                                                                                                                                           >  1:692466/1‑150 (MQ=255)

          cTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGca                                                                                                                                           >  1:3031246/1‑150 (MQ=255)

           tAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGcac                                                                                                                                          >  1:1043650/1‑150 (MQ=255)

             gCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAg                                                                                                                                        <  2:2155076/150‑1 (MQ=255)

              cAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGc                                                                                                                                       >  1:379360/1‑150 (MQ=255)

               aaTTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCg                                                                                                                                      <  2:3171324/150‑1 (MQ=255)

               aaTTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCg                                                                                                                                      <  2:4026009/150‑1 (MQ=255)

               aaTTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCg                                                                                                                                      <  2:3726474/150‑1 (MQ=255)

                aTTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTATCCCTGAAAAGACGCACcgcga                                                                                                                                     >  1:4030728/1‑149 (MQ=255)

                 ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                    <  2:1706947/150‑1 (MQ=255)

                    gtCACTGACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                                                                 <  1:499908/149‑1 (MQ=255)

                      cACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                                                                               <  1:5339302/150‑1 (MQ=255)

                      cACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGATAGTTGGCCTGATAATACGAAAATCGTCTCAta                                                                                                                               >  2:4290395/1‑149 (MQ=37)

                       aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCTTCa                                                                                                                              >  1:5434835/1‑150 (MQ=255)

                       aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                                                              >  1:1301515/1‑150 (MQ=255)

                        cTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                                             <  1:2612052/150‑1 (MQ=255)

                               ggTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACgg                                                                                                                      >  2:5372089/1‑150 (MQ=255)

                                gTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTTTAGCCCTAATAAGACCCACACCGACCCAACAGCAAACgca                                                                                                                     >  1:3675072/1‑148 (MQ=37)

                                gTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGc                                                                                                                     >  1:1846680/1‑150 (MQ=255)

                                gTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGc                                                                                                                     >  1:5194561/1‑150 (MQ=255)

                                 ttAAGACTCATGTTTTTTGCTACTCGTTAGTTACTGCAGTTGTGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGTTTTTGTTCCGTTTTGTTGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCt                                                                                                                    <  1:2716428/150‑1 (MQ=21)

                                  tAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                                                   >  1:3337098/1‑150 (MQ=255)

                                     gACTCATGGTTTTTGCTACTCTTTTGTTACTGCTGTTGTGGATTTTTTACGGCTGCGGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATTTTCCGTTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCg                                                                                                                <  2:2561594/150‑1 (MQ=21)

                                         caaggTTTTTGCTACTCTTTAGTTACTGCAGTAGTGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTAGGTTCCGGTTTGTAGGCATGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATg                                                                                                            <  2:918317/146‑1 (MQ=21)

                                         cATCGTTTTTGCTCCTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATg                                                                                                            <  2:3551528/150‑1 (MQ=255)

                                          aTCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgc                                                                                                           >  1:4456127/1‑150 (MQ=255)

                                          aTCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgc                                                                                                           >  2:876566/1‑150 (MQ=255)

                                          aTCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCATACGGTTAACATCCGGTTAGTAGGCCTGAAAATACTCACAGCGTCGCATCAGGCAACGGCTGTCGGATgc                                                                                                           >  1:4426861/1‑150 (MQ=25)

                                                    cTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACg                                                                                                 >  1:5506300/1‑150 (MQ=255)

                                                    cTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTATCGGATGCGGCGTAAACg                                                                                                 >  1:793933/1‑150 (MQ=255)

                                                     tACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAATACGAACAGCGTCGCATAAGGCAAAGGCTGTCGGATCCGGCTTAAACGc                                                                                                >  2:1915651/1‑150 (MQ=21)

                                                         cggtagttaCTGCTGTCGAGTATTTTTTCGGGCTGCCGTTTGCGGCGTGAACGCCCTATCCAGCATAGGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTa                                                                                            <  2:4875447/147‑1 (MQ=18)

                                                         cGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTa                                                                                            >  1:4617359/1‑150 (MQ=255)

                                                          gttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTAt                                                                                           >  1:629093/1‑150 (MQ=255)

                                                           ttagttaCTGCAGTAGAGGTTTTTTTCCGGCGGCCGGAGGCGGCGTGAACGCCCTAACCAGCCTACGGTTATGTTCCGGTTTGTTGGCCTGATAAGCCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                          <  2:2162621/150‑1 (MQ=18)

                                                            tagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                         >  1:4169427/1‑150 (MQ=255)

                                                             agttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTAATCCg                                                                                        >  1:1447492/1‑150 (MQ=255)

                                                              gttaCTGCATTAGAGTATTTTTTCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGTTTTGTAGGCCTGATAAGACGCAAAGCGTCGAATCCGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGa                                                                                       <  2:1071520/150‑1 (MQ=18)

                                                               ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACTGCTGACGGATCCGGAGTAAACGCCTTATCCGAc                                                                                      >  2:4821052/1‑150 (MQ=255)

                                                               ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                      >  2:4713003/1‑150 (MQ=255)

                                                               ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                      >  1:3299340/1‑150 (MQ=255)

                                                                 aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGGCCTACGGTTATGTTCTGGATTGTTGGCCTGTTGATAGGAACAGCGTCGATTAAGGCAAGTGGTTTCGGATGCGGCGTAATCTCATTGTCCGagca                                                                                    >  2:2371222/1‑147 (MQ=12)

                                                                  cTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGATGCGGCGTGAACGCCCTCTCCGACCTa                                                                                   >  2:674672/1‑150 (MQ=17)

                                                                      aGTAGAGTATTTTTTCCGGCTGCCGGTTGGGGCGTGAACGCCCTATCCAGCCTACGGTTTTGTTCCGGTTTGTAGGCCTTATAAGCCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                               <  2:1527674/150‑1 (MQ=17)

                                                                      aGTAGAGTATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                               <  2:2750281/150‑1 (MQ=255)

                                                                      aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCCGGGTTGTAGGCCTGATACGACGCACAGCCACGCATCACGCAACGGCACTCGGAGGCGGCGTAAACGCCTTAACCGACCTACGGt                                                                               >  1:2124612/1‑150 (MQ=18)

                                                                          gagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                                                           >  1:4062616/1‑150 (MQ=255)

                                                                              aTTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCATCCTACGGTTATGTTCCGGTTTTTAGGCATGATAAGACGCACAGCGTGGCATAAGGCAACGGATCACTGATGCGGCGTAAACGCCTTATCCGACCTACGGTTTTGTTcc                                                                       >  1:2813291/1‑150 (MQ=17)

                                                                                  tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTAGGGTTATGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                   <  2:5534071/150‑1 (MQ=255)

                                                                                    tACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGt                                                                 >  1:1700385/1‑150 (MQ=255)

                                                                                        gCTGCCGGATGCGGCGTGAACGCCCTACCCAGCCTACGGTTATGTTCCGGTTGGTAGGCCTTATAAGACGCACCGCGTCGAAACAGCCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                             <  1:1786695/150‑1 (MQ=17)

                                                                                          tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCCGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGGTATGTTCCGTTTGTAGGCCt                                                           >  2:2075395/1‑150 (MQ=255)

                                                                                           gcggtttGCGGCGTTAACGCCCTACCCCGCCTAGGGTTAGGTTCGGGTTTGTCGGCCTTATACGCCGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                          <  1:1342241/144‑1 (MQ=14)

                                                                                            ccGGTTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCGGGTTTGTCGGCCTTAAAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                         <  1:5515970/150‑1 (MQ=14)

                                                                                            ccGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                         >  2:3279481/1‑150 (MQ=255)

                                                                                            ccGGAGGCGGCGTGAACGCCCTATCCAGCCTCCGGTTATGTTCCGGTTGGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                         <  2:5079903/150‑1 (MQ=255)

                                                                                             cGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGAt                                                        >  1:3161982/1‑150 (MQ=255)

                                                                                             cGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                        >  2:4447951/1‑150 (MQ=255)

                                                                                             cGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                        >  1:4755971/1‑150 (MQ=255)

                                                                                              ggATGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCAGGGTAGTAGGCCTGATAACACGAAAACCCACGAATCACCAACCGTCACACGAATCCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGaga                                                       >  1:5275151/1‑148 (MQ=9)

                                                                                              ggATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCAGATa                                                       >  1:3118318/1‑150 (MQ=255)

                                                                                              ggATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTGGTAGGCCTGATAAGACGCACAGCGTCGCCTCCGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                                                       >  1:3958238/1‑150 (MQ=255)

                                                                                               ggtGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                                      >  1:225605/3‑150 (MQ=255)

                                                                                               gATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                                      <  2:5030156/150‑1 (MQ=255)

                                                                                               gATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGATGTAGGCCTGATaa                                                      >  2:4593520/1‑150 (MQ=255)

                                                                                               gATGCGGCGTGAACGCCCTAGCCAGCCTACGGTTATGTACCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTCTCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                                      <  2:2268311/150‑1 (MQ=255)

                                                                                                agggggggTGCACGCCCAAACCAGCATAGGGTTATGTGCCGGTTTTTAGGCCTGATAAGACCCACCGCGTCGCATCAGGCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAg                                                     <  2:2322276/143‑1 (MQ=14)

                                                                                                  gcggcgTTAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTGGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                                                   <  2:82147/150‑1 (MQ=255)

                                                                                                  gcggcgTGAACGCCATAACCAGCCTAGGTTTATGTTCCGTTTTTTTGGCCTTATAATACCCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                                                   <  1:1177276/150‑1 (MQ=14)

                                                                                                     gcgTGACCGCCCTATCCAGCCTACGGTTTTGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                <  1:3620643/150‑1 (MQ=255)

                                                                                                     gcgTGAACGCCCTACCCAGCCTACGTTTTTGTTCCGTTTTGTAGGCCTTAAAAGACGCACCGCGTCGCATCCGCCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                <  1:855664/150‑1 (MQ=14)

                                                                                                       gTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                              >  2:3506434/1‑150 (MQ=255)

                                                                                                       gTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                              >  1:2509968/1‑150 (MQ=255)

                                                                                                        tGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAg                                             <  2:3337098/150‑1 (MQ=255)

                                                                                                        tGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGGAGGCCTGATAAGACGCACAGCGTCGCATCACGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAg                                             <  2:4137241/150‑1 (MQ=255)

                                                                                                         gAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGc                                            >  2:4646035/1‑150 (MQ=255)

                                                                                                           aCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGt                                          >  1:424322/1‑150 (MQ=255)

                                                                                                            cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                         <  2:3916676/150‑1 (MQ=255)

                                                                                                             gCCCTATCCAGCCGAGGGTTAGGTTGCGTTTTGTCGGCGTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                        <  2:3827678/150‑1 (MQ=14)

                                                                                                              cccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                       >  2:8001/1‑150 (MQ=255)

                                                                                                                cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTTGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                     >  1:4181082/1‑150 (MQ=255)

                                                                                                                 tATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                    >  1:3871616/1‑150 (MQ=255)

                                                                                                                 tATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGAGTCGCATc                                    >  2:3387546/1‑150 (MQ=255)

                                                                                                                  aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                   <  2:607162/150‑1 (MQ=255)

                                                                                                                   cccAGCCTAGGGTTATGTTCCGGTTTGTCGGCCTTATAAGACGCACCGCGTCGCATCCGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                  <  1:5144015/149‑1 (MQ=14)

                                                                                                                    cccgCCTAGGTTTTTGTTCGGGTTTGTGGGCGTGATACGACGCCCCGCGTCGCATCCGGCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                 <  1:5186991/147‑1 (MQ=11)

                                                                                                                     cAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGc                                >  2:1274201/1‑150 (MQ=39)

                                                                                                                      aGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCa                               <  1:3631569/150‑1 (MQ=38)

                                                                                                                       gCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGGTCCGTATGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCaa                              >  2:2945034/1‑150 (MQ=255)

                                                                                                                                 aTGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCgg                    >  2:3151167/1‑150 (MQ=37)

                                                                                                                                        ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc             >  1:709276/1‑150 (MQ=32)

                                                                                                                                        ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc             >  1:5400678/1‑150 (MQ=32)

                                                                                                                                              tAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAAc       >  2:417495/1‑150 (MQ=34)

CCGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTT  >  CP009273/3385344‑3385640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`