BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,339	A→C	9.7%	Q12P (CAA→CCA) ‡	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,339	0	A	C	9.7%	676.5 / 7.5	282	Q12P (CAA→CCA) ‡	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (154/91); new base C (31/6); total (185/97)
Fisher's exact test for biased strand distribution p-value = 1.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488

tatataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCa                                                                                                                                                       >  1:2978040/1‑150 (MQ=255)

tatataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                                                                                                                       <  1:636632/150‑1 (MQ=255)

  tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                     >  2:5105356/1‑150 (MQ=255)

   ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  1:1240892/1‑150 (MQ=255)

     aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGACAATGCGCAACCgg                                                                                                                                                  >  2:1287865/1‑150 (MQ=255)

      aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  2:3692613/150‑1 (MQ=255)

      aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 >  2:2265360/1‑150 (MQ=255)

      aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGt                                                                                                                                                 >  1:924167/1‑150 (MQ=255)

        aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTg                                                                                                                                               >  2:5357910/1‑150 (MQ=255)

        aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                               >  1:3574309/1‑150 (MQ=255)

          aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgcg                                                                                                                                             >  2:2703989/1‑150 (MQ=255)

             gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAg                                                                                                                                          <  2:1275785/150‑1 (MQ=255)

               aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGAc                                                                                                                                        >  1:1573330/1‑150 (MQ=255)

                 ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTc                                                                                                                                      >  2:1420605/1‑150 (MQ=255)

                  tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                     >  2:5219741/1‑150 (MQ=255)

                      tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                 >  2:4266068/1‑150 (MQ=255)

                       cATTTAAATATTTAGTTGAGGTTGTGGCAAACGTTTGCTTTTCTCTTGCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                <  2:312644/150‑1 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  2:2055450/1‑150 (MQ=255)

                         tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACaaaaaacc                                                                                                                              >  1:301363/1‑146 (MQ=255)

                         tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACacc                                                                                                                              >  1:4994581/1‑148 (MQ=255)

                         tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACacc                                                                                                                              >  1:3068035/1‑148 (MQ=255)

                           tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAACACCGa                                                                                                                            >  1:3876333/1‑150 (MQ=255)

                           tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                            >  2:5347332/1‑150 (MQ=255)

                           tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                            >  2:2431091/1‑150 (MQ=255)

                           tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACaaaaaaacca                                                                                                                            >  1:3712762/1‑144 (MQ=255)

                            aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACACCGaa                                                                                                                           >  1:200356/1‑150 (MQ=255)

                             aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          >  2:851602/1‑150 (MQ=255)

                              atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAACCGAACt                                                                                                                         >  1:1670466/1‑150 (MQ=255)

                              atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGAGCAGACACAAAACaaccaaaa                                                                                                                         >  1:2870796/1‑143 (MQ=255)

                               tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCAACCGGTTGCGCCGAAACAAAAAAACGaaaat                                                                                                                        >  1:5154915/1‑147 (MQ=255)

                                atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTgg                                                                                                                       >  1:4917978/1‑149 (MQ=255)

                                  ttAGATGTGTTTGTGGAAACGGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCCCGGGATTGTTTTCGTTGTCTTTTTCCACCCTCGAGTCAGAAAAGGCGCAAGGGGTTGCGCAGACTCAAAACAGCGAACTGAtt                                                                                                                     <  1:349053/150‑1 (MQ=255)

                                  ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAtt                                                                                                                     >  2:3948524/1‑150 (MQ=255)

                                     gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCACAAAACCGAACAGATTTaa                                                                                                                  >  1:2777009/1‑149 (MQ=255)

                                       tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACTCAAAACAGCGAACTGATTTACCg                                                                                                                >  2:1022739/1‑150 (MQ=255)

                                        gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                               <  2:3410516/150‑1 (MQ=255)

                                         aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCCGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTGTCGCTGTCTGTTTCCACCCTCGAGTCTGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  2:2519996/150‑1 (MQ=255)

                                          ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                             >  2:4788673/1‑150 (MQ=255)

                                            ttgtgGAAATCGTTGGCTTTTCTCTTGCGGACAGATAAATGGCCCGATTGGCTACCAGGGTTTTGTTTTCTTGGTGTGTTCCCACCGGCGTGCCAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTg                                                                                                           <  1:1707446/149‑1 (MQ=255)

                                             tgtgGAAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                          <  2:4095171/150‑1 (MQ=255)

                                                   aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    >  2:5019613/1‑150 (MQ=255)

                                                       gTTTGCTTTTCTCTTGCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                                <  2:233823/150‑1 (MQ=255)

                                                          tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAGCGAACAGATTTAACGTCTTGAAGATAGTacgccg                                                                                             >  1:3809492/1‑150 (MQ=255)

                                                          tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCCCAGACTCAAAACACCGAACAGATTTAACCTCTTGAAGATCGTccgccc                                                                                             >  1:3488151/1‑149 (MQ=255)

                                                            cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                           >  2:567185/1‑150 (MQ=255)

                                                            cTTTTCTCTGTCGGTCAGCTAAACTGCCCGCTTGGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                           <  2:2411414/150‑1 (MQ=255)

                                                             ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                          <  2:4039076/150‑1 (MQ=255)

                                                             ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGCACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                          >  1:5026973/1‑150 (MQ=255)

                                                                tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACTCAAAAAAGCGGACTGATTTAACGTCTTTAATATAGTGCGCCCCGTcca                                                                                       >  1:2789268/1‑149 (MQ=255)

                                                                 ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCCGACACAAAAAAGCGAACAGATTTAACGTCATTAATATAGTCCGCCCCGTccac                                                                                      >  1:2145723/1‑148 (MQ=255)

                                                                  tgtttCGGTCAGCTAAAATGCCCGCTTTGATACCACGGGATTGTTTTCGTTGCCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                     <  2:943230/148‑1 (MQ=255)

                                                                       cGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTGTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCAGACTCAAAACAGCGAACGGAGGTACCGTCGTGAAGATCCTCCGCCGCGTCCGCAAAccc                                                                                >  2:4210880/1‑150 (MQ=255)

                                                                         gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcccag                                                                              >  1:133399/1‑148 (MQ=255)

                                                                         gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                              >  2:1850005/1‑150 (MQ=255)

                                                                           cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACACAAAACACCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAAcccgggt                                                                            >  1:3290114/1‑146 (MQ=255)

                                                                               tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAAACCTGTTTgcc                                                                        >  1:1521738/1‑150 (MQ=255)

                                                                                aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTCCCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGGTgccg                                                                       >  2:4257301/1‑150 (MQ=255)

                                                                                    tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTg                                                                   >  2:2988498/1‑150 (MQ=255)

                                                                                         gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                              <  2:2889647/150‑1 (MQ=255)

                                                                                         gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACACAAAAAAGCGAACTGATTTAACGTCTTTAAGATCGTCCGCCGCGTCCACAAACCCAGTTTTCCGCCAGTCAGc                                                              >  1:242432/1‑150 (MQ=255)

                                                                                          cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTACGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCa                                                             >  2:5275360/1‑150 (MQ=255)

                                                                                           tttGCTACCACGGGATTGTTTTCGTTGTCTGTGTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAt                                                            <  2:4003414/150‑1 (MQ=255)

                                                                                            ttGCTACCACGGGAGTGTTTTCGATGTCTGGTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGCACTGATTTACCGTCGTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGGCGCCTGTCAGCATc                                                           >  2:2911561/1‑150 (MQ=255)

                                                                                             tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  2:1937998/150‑1 (MQ=255)

                                                                                                tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAAATGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCACAAACCCTGTTTGCCGCCGGTCAGCAAatgct                                                       >  1:2283064/1‑150 (MQ=255)

                                                                                                 aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                      >  2:2660223/1‑150 (MQ=255)

                                                                                                      gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                 >  2:1700427/1‑150 (MQ=255)

                                                                                                       ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACACAAAAAAGCGAACAGATTTACCGTCATGAAGATCGTCCGCCCCGTCCGCAAAACCGGGTTGGCGCCGGTCAGCAACATCTGGCgcag                                                >  1:5378485/1‑147 (MQ=255)

                                                                                                        ttttGTTTTCGATGTCTGTTACCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                               <  1:5404513/148‑1 (MQ=255)

                                                                                                         aTTGTTTTCGATGTCTGTTTCCACCCGCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                              <  2:737553/150‑1 (MQ=255)

                                                                                                           tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACAAAAAACAGAGAACTGATTTACAGGCATGAAGATCGTCCGCCGCTTCCTCAAAACCAGTTTTGCGCCTGTCAGCATCAGCTGGCGATGTTCg                                            >  1:439635/1‑150 (MQ=255)

                                                                                                              tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                         <  2:2846311/150‑1 (MQ=255)

                                                                                                                tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                       <  2:5418719/150‑1 (MQ=255)

                                                                                                                tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                       <  2:4949040/150‑1 (MQ=255)

                                                                                                                 cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACAAAAAACAACGAAATGATTTACCGGCTTGAAGATCGGCCCCCGCGTCCTACAACCCAGTTTGGCGCCATACAGCAAAAGCTGGCGCAGGTCGTTGCgg                                      >  1:4034500/1‑150 (MQ=255)

                                                                                                                    tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   >  2:4157139/1‑150 (MQ=255)

                                                                                                                       cTGTTACCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  1:5328009/150‑1 (MQ=255)

                                                                                                                        tGTTACCACCCTCTATTCATAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  1:2055450/150‑1 (MQ=255)

                                                                                                                         gTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACACCGAACAGATTTAAAGGCTTTAAGATAGTCCGCCCCGTCCTCAAAACCAGTTTTGCGCCAGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGATAACg                              >  1:4872620/1‑150 (MQ=255)

                                                                                                                          tttCCACCCTCGAGTCATAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  1:1007515/150‑1 (MQ=255)

                                                                                                                          tttCCACCCTCGAGTCACAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  1:1007437/150‑1 (MQ=255)

                                                                                                                            tCCCCCCGCGAGTCAGAACAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  1:3105221/150‑1 (MQ=255)

                                                                                                                            tCCACCCTCCAGTCAGAAAATGCGCCCCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  2:4386052/150‑1 (MQ=255)

                                                                                                                             ccccccTCGAGTCATAAATGGCGCACCGGGGTCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                          <  1:4266068/147‑1 (MQ=255)

                                                                                                                               aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCGGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGATAACGCCAgcg                        >  1:2309282/1‑150 (MQ=255)

                                                                                                                               aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCCGCATCTGCTGGCGATGTTCGTTGCGGTGCTCACGCCAgcg                        >  2:5519732/1‑150 (MQ=255)

                                                                                                                               aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAAAAGCGAACTGATTTAACGTATTTAATATCGTCCGCCGCTTCCTCAAAACCAGTTTTCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTTCGGTGATAAcccccccg                        >  1:2845943/1‑146 (MQ=255)

                                                                                                                                  atctaGTCATAAAAGGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                     <  1:4658192/146‑1 (MQ=255)

                                                                                                                                    cGAGTCATAAAAGGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  1:4822318/150‑1 (MQ=255)

                                                                                                                                    cGAGTCAGAAAATGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  1:3335633/150‑1 (MQ=255)

                                                                                                                                      aGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa                 >  2:3546474/1‑150 (MQ=255)

                                                                                                                                       gTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCGTTAAGATCGTCCGCCGCGTCCTCAAAACCAGTTTGCCGCCAGTCAGCAACTGCTGGCGATGTTCGTTGCGGTGATCACGCCACCGCTATTaaa                >  1:3422634/1‑149 (MQ=255)

                                                                                                                                        tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc               <  2:3793714/150‑1 (MQ=255)

                                                                                                                                          aGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             <  1:842929/150‑1 (MQ=255)

                                                                                                                                          aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             >  2:4490564/1‑150 (MQ=255)

                                                                                                                                            aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  2:5497627/1‑150 (MQ=255)

                                                                                                                                            aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  2:3293954/150‑1 (MQ=255)

                                                                                                                                            aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  2:1546470/1‑150 (MQ=255)

                                                                                                                                                   gcACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt    <  2:21967/150‑1 (MQ=255)

                                                                                                                                                     aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:1131406/1‑150 (MQ=255)

TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`