Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,346 T→G 6.5% V14V (GTT→GTG) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3460TG 6.5% 850.9 / 8.0 281V14V (GTT→GTG) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base T (180/73);  new base G (3/23);  total (184/97)
Fisher's exact test for biased strand distribution p-value = 3.16e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

AAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTACC  >  CP009273/3822198‑3822495
                                                                                                                                                    |                                                                                                                                                     
 aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                                                                                                                                     <  1:944637/150‑1 (MQ=255)
  aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                                    >  2:4876976/1‑150 (MQ=255)
     gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAg                                                                                                                                                 >  1:5215096/1‑150 (MQ=255)
     gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAg                                                                                                                                                 <  2:1276163/150‑1 (MQ=255)
       aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGAc                                                                                                                                               >  2:4093524/1‑150 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                            >  2:5219741/1‑150 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                            >  2:2401581/1‑150 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCa                                                                                                                                            >  1:3865527/1‑150 (MQ=255)
            tctcATTAAAATATTTAGATGAGGATGTGGCAAACCTTTGCTAATCTCTTTCGCTCAGCTAAAATGCACGCTTTGCTACAACGCGAAAGTATTCGATGTCTGTTTCCACCCTCGATTCCGAAAATGCTCAACCGGAAGCGCACACTCaaa                                                                                                                                          >  2:4513589/1‑150 (MQ=255)
              tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                        >  2:4266068/1‑150 (MQ=255)
               cATTTAAATATTTTGATGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCTGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACa                                                                                                                                       <  2:311417/150‑1 (MQ=255)
                aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAg                                                                                                                                      >  2:2055450/1‑150 (MQ=255)
                   tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAACACCGa                                                                                                                                   >  1:3876333/1‑150 (MQ=255)
                   tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                                   >  2:5347332/1‑150 (MQ=255)
                    aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACACCGaa                                                                                                                                  >  1:200356/1‑150 (MQ=255)
                     aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                                 >  2:851602/1‑150 (MQ=255)
                      atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAACCGAACt                                                                                                                                >  1:1670466/1‑150 (MQ=255)
                       tatTTTGAGGAGGATGTGGAAAACGTTGGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                               <  2:3954784/150‑1 (MQ=255)
                        atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCACACTCAAAACACCGAACAGa                                                                                                                              >  1:3012272/1‑150 (MQ=255)
                        atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAGCGAACAGa                                                                                                                              >  1:4918136/1‑150 (MQ=255)
                          ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAtt                                                                                                                            >  2:3948524/1‑150 (MQ=255)
                             gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCACAAAACCGAACAGATTTaa                                                                                                                         >  1:2777009/1‑149 (MQ=255)
                                 aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                                     >  2:1081762/1‑150 (MQ=255)
                                    ttgtgGAAATCGTTGGCTTTTCTCTTGCGGACAGATAAATGGCCCGATTGGCTACCAGGGTTTTGTTTTCTTGGTGTGTTCCCACCGGCGTGCCAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTg                                                                                                                  <  1:1707446/149‑1 (MQ=255)
                                      gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                                >  2:2724416/1‑150 (MQ=255)
                                        ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACACCGACCGGATTTACCGTCTGGAAGa                                                                                                              >  1:3181331/1‑150 (MQ=255)
                                         gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACAGACTTGAatat                                                                                                             >  2:538366/1‑150 (MQ=255)
                                          cAACCGTTTGCTTTTATCTTTCGGTCAGATAAAAGGCCCGCTTTGCTACCAGGGTATTTTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                            <  1:3615013/150‑1 (MQ=255)
                                           aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTCCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACGCAAAACAGCGAACTGATTTACCGTCTTGAATATCg                                                                                                           >  2:33475/1‑150 (MQ=255)
                                           aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                           >  2:1631033/1‑150 (MQ=255)
                                           aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCg                                                                                                           >  2:5019624/1‑150 (MQ=255)
                                            aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGACGCAAAAAAGCGAACTGATTTAACGTATTTAAGATAGt                                                                                                          >  1:35068/1‑150 (MQ=255)
                                                  tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCCCAGACTCAAAACACCGAACAGATTTAACCTCTTGAAGATCGTccgccc                                                                                                    >  1:3488151/1‑149 (MQ=255)
                                                    cgtggcgctTGCGGTCAGATAAATGGCCCGGTTTCCTACCTGGGGTTTTTTTTCTTTGTGTGTTACCACCCACGAGTCATAAATTGCGCCCCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                                  <  1:5378719/143‑1 (MQ=255)
                                                    cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                                  <  2:2412903/150‑1 (MQ=255)
                                                    cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                                  >  2:567185/1‑150 (MQ=255)
                                                     ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGGCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACATCGAACGGAGTTAACGTCTTGTAGAGAGTCCGCCGCtt                                                                                                 >  1:5026456/1‑150 (MQ=255)
                                                        tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACTCAAAAAAGCGGACTGATTTAACGTCTTTAATATAGTGCGCCCCGTcca                                                                                              >  1:2789268/1‑149 (MQ=255)
                                                        tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGACGATCGTCCGCCGCTTCCt                                                                                              >  2:1204318/1‑150 (MQ=255)
                                                         ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGAGCAGACTAAAAAAAGCGAACATATTTAACGTCTTGAATATAGTCAGCCGCTTCCTc                                                                                             >  1:2951897/1‑150 (MQ=255)
                                                          tgtttCGGTCAGCTAAAATGCCCGCTTTGATACCACGGGATTGTTTTCGTTGCCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                            <  2:943230/148‑1 (MQ=255)
                                                          tgtgtCGGTCAGATAAATTGCCCGTTTTCATACCTGGTTTTTGTTTTCTTTTTGTGTTTCCCCCCTCGAGAAATAAAATGCGCCCGCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                            <  1:3328840/146‑1 (MQ=255)
                                                               cGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTGTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCAGACTCAAAACAGCGAACGGAGGTACCGTCGTGAAGATCCTCCGCCGCGTCCGCAAAccc                                                                                       >  2:4210880/1‑150 (MQ=255)
                                                                ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCTTCCTCAAACCCt                                                                                      >  2:2332813/1‑150 (MQ=255)
                                                                  tCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCGGGGGCGCCGACCCAAAAAAGCGCACTGATTTTCCGTCTTGAAGATCGTCCGCCGCGTCCACAAAaccggg                                                                                    >  1:1649415/1‑147 (MQ=255)
                                                                       tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCCGACACAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCGCAAAACCGGTTTgcc                                                                               >  1:4822410/1‑150 (MQ=255)
                                                                        aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGTTCGTCCGCCGCGTCCTCAAACCCTGTTTgccg                                                                              >  2:4257369/1‑150 (MQ=255)
                                                                           aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCt                                                                           >  2:4980876/1‑150 (MQ=255)
                                                                           aTGCCCGCTGTGCTACCCCGGGCGTGGTGGCGAGGTCTGTGTCCACCCTCGAGGCAGACAATGCGCAACCGGTTGCGCAGACTCTAAACCGCGAGCTGCGGTCCCGGCTTGCAGCTCGTCCGCCGCGGCCTCAAACCCGGTGTgccgccg                                                                           >  2:1450051/1‑149 (MQ=255)
                                                                                 gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                                     <  2:2889647/150‑1 (MQ=255)
                                                                                 gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTTTAGATAGTCCGCCGCGTCCTCAAAACCAGTTTGCCGCCGGTCAGc                                                                     >  1:5047322/1‑150 (MQ=255)
                                                                                 gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACACAAAAAAGCGAACTGATTTAACGTCTTTAAGATCGTCCGCCGCGTCCACAAACCCAGTTTTCCGCCAGTCAGc                                                                     >  1:242432/1‑150 (MQ=255)
                                                                                  cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTACGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCa                                                                    >  2:5275360/1‑150 (MQ=255)
                                                                                    gtGCTACCTGGGGTTTGTTTTCGTTGTCTGTTTCCCCCCTCGAGTCATAAACTGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                                  <  1:4309128/149‑1 (MQ=255)
                                                                                         aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                             >  2:2660572/1‑150 (MQ=255)
                                                                                          cctgggtaTTGTTTTCGTTGTGTGTTCCCACCCTCGAGTAATAAACTGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                            <  1:488866/143‑1 (MQ=255)
                                                                                              gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGAGCAGACTCAAAAAAGCGAACAGATTTAACCTCTTGAATATAGTCCGCCGCTTCCACAAAACCAGTTTGCCGCCAGTCAGCAACTGCTGGCGAt                                                        >  1:3074223/1‑150 (MQ=255)
                                                                                              gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                        >  2:2564022/1‑150 (MQ=255)
                                                                                               gtttttgtttCGTTGTCTTTTCCCACCCCCGAGTCATAACATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                       <  1:4811786/143‑1 (MQ=255)
                                                                                                gATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTAAAAACAACGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCATCCACAAACCCAGTTTGCCGCCAGTCAGCAAAAGCAGGCGATGt                                                      >  1:2296577/1‑150 (MQ=255)
                                                                                                 aTTGTTTTCGATGTCTGTTTCCACCCGCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                                     <  2:737553/150‑1 (MQ=255)
                                                                                                   tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTTCGCAGACTCAAAACAGCGAACTGATTTACCGTATTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                                   >  1:439657/1‑150 (MQ=255)
                                                                                                   tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGTTTCGCCGACACAAAACAGCGAACATATTTAACCGCATTTAGATAGTCCGCCGCGTCCTCAAAACCTGGTTTCCGCCGGTGAGCATCTGCAGGCGATGTTCg                                                   >  1:55403/1‑150 (MQ=255)
                                                                                                    gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAAAACCGAACTGATTTACCGTCTTTAAGATCGTCCGCCGCTTCCACAAAACCTGTTTGCCGCCGGTCCGCATCAGCTGGCGATGTTCGt                                                  >  1:706680/1‑150 (MQ=255)
                                                                                                      tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                                >  2:2503549/1‑150 (MQ=255)
                                                                                                      tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                                <  2:2846311/150‑1 (MQ=255)
                                                                                                         cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTTAAGATAGTCCGCCGCTTCCTCAAACCCAGTTTTCCGCCTGTCAGCAACTGCTGGCGATGTTAGTTGCgg                                             >  1:3059469/1‑150 (MQ=255)
                                                                                                         cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACAAAAAACAACGAAATGATTTACCGGCTTGAAGATCGGCCCCCGCGTCCTACAACCCAGTTTGGCGCCATACAGCAAAAGCTGGCGCAGGTCGTTGCgg                                             >  1:4034500/1‑150 (MQ=255)
                                                                                                           ttttgtGTTTCCCCCCTCGTGTAAGAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGATTCTGCTGGCGATGTTCGTTGCtgtg                                           <  2:292475/145‑1 (MQ=255)
                                                                                                            tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                          >  2:4157139/1‑150 (MQ=255)
                                                                                                             gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGAAACAAAAAAACGAACAGATTTACCGTATTGTATATAGTCCGCCCCTTCCTCAAAAACAGTTTTGCGCCGGTCATCAACTGATGGCGATTTTAGTTGGGGTGAt                                         >  1:1421274/1‑150 (MQ=255)
                                                                                                             gTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                         <  2:1837850/150‑1 (MQ=255)
                                                                                                               cTGTTACCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                       <  1:5328009/150‑1 (MQ=255)
                                                                                                                tGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGAAACAAAACATCGAAAAGATTTAAACACTTGAAGAAAGTCCCCCCCTTCCACAAACCCAGGTTTCCGCCAGTCAGCAACAGCTGGCGATGTTCGTTTCGGTGATCaa                                      >  1:3392260/1‑149 (MQ=255)
                                                                                                                  tttCCACCCTCGAGTCATAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                                    <  1:1007515/150‑1 (MQ=255)
                                                                                                                  tttCCACCCTCGAGTCACAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                                    <  1:1007437/150‑1 (MQ=255)
                                                                                                                  ttaccACCCTCGAGTCACAAAATGCGCCACGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                                    <  1:2272196/147‑1 (MQ=255)
                                                                                                                   tcccACCCTCGAGTCAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                                   <  1:1529019/148‑1 (MQ=255)
                                                                                                                    tCCACCCTCCAGTCAGAAAATGCGCCCCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                                  <  2:4386052/150‑1 (MQ=255)
                                                                                                                      cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACAGATTTAACGTCTTGAAGATAGTCCGCCCCTTACTCAAACCCAGTTTTCCGCCTGTCAGCATAAGCTGGCGCTGTTAGTTGCGGTGATAACCCCAgc                                >  1:1269648/1‑150 (MQ=255)
                                                                                                                       aCCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                               <  2:3265315/150‑1 (MQ=255)
                                                                                                                       aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCGGTTTTCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGATAACGCCAgcg                               >  1:2309282/1‑150 (MQ=255)
                                                                                                                       aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCCGCATCTGCTGGCGATGTTCGTTGCGGTGCTCACGCCAgcg                               >  2:5519732/1‑150 (MQ=255)
                                                                                                                       aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAAAAGCGAACTGATTTAACGTATTTAATATCGTCCGCCGCTTCCTCAAAACCAGTTTTCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTTCGGTGATAAcccccccg                               >  1:2845943/1‑146 (MQ=255)
                                                                                                                              aGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa                        >  2:3546474/1‑150 (MQ=255)
                                                                                                                               gTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCGTTAAGATCGTCCGCCGCGTCCTCAAAACCAGTTTGCCGCCAGTCAGCAACTGCTGGCGATGTTCGTTGCGGTGATCACGCCACCGCTATTaaa                       >  1:3422634/1‑149 (MQ=255)
                                                                                                                                tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                      <  2:3793714/150‑1 (MQ=255)
                                                                                                                                 cAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTACTCAAACCCTGTTTGCCGCCTGTCAGCATCAGCTGGCGCTGTTCGTTGCGGTGATCACGCCCGCGCTATTAATCt                     >  2:1916677/1‑150 (MQ=255)
                                                                                                                                 cAGAAAAGGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt                     <  1:3752538/150‑1 (MQ=255)
                                                                                                                                    aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc                  >  2:1546470/1‑150 (MQ=255)
                                                                                                                                    aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc                  >  2:5497731/1‑150 (MQ=255)
                                                                                                                                      actGCGCAACCGGTTGCGCAGACTCAAAACCGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTGCGTTGCGGTGATCACGCCAGCGCTATTCATCGGcccg                >  2:4017380/3‑148 (MQ=255)
                                                                                                                                          cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc            >  1:2073229/1‑150 (MQ=255)
                                                                                                                                           gcACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt           <  2:21967/150‑1 (MQ=255)
                                                                                                                                           gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt           <  2:5474081/150‑1 (MQ=255)
                                                                                                                                             accgggTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg         <  1:4135623/146‑1 (MQ=255)
                                                                                                                                             aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg         >  2:1131406/1‑150 (MQ=255)
                                                                                                                                                 ggTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGttt     <  2:2978084/150‑1 (MQ=255)
                                                                                                                                                  gTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa    <  2:3412732/150‑1 (MQ=255)
                                                                                                                                                    tGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTAcc  <  2:3712762/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTACC  >  CP009273/3822198‑3822495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑