Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,511 A→C 5.6% V323V (GTT→GTG)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,5110AC 5.6% 994.9 / 12.6 328V323V (GTT→GTG) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base A (154/147);  new base C (4/23);  total (158/170)
Fisher's exact test for biased strand distribution p-value = 2.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

CAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGG  >  CP009273/3872363‑3872660
                                                                                                                                                    |                                                                                                                                                     
cAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGaaa                                                                                                                                                      >  2:4670687/1‑150 (MQ=255)
cAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGaaa                                                                                                                                                      >  1:4271575/1‑150 (MQ=255)
  aCCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACg                                                                                                                                                    >  2:4427840/1‑150 (MQ=255)
  aCCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACg                                                                                                                                                    >  2:2390882/1‑150 (MQ=255)
  aCCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTAATCTGCTGTTCAACCGCCGATTTCACCTCAGAATAAACCAGTTTGTCTTTGGTATGGGAGGAGAATTTAGGGTCCGGCACATTCACGGAAACg                                                                                                                                                    >  1:1011230/1‑150 (MQ=255)
    cACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGAc                                                                                                                                                  <  1:4225577/150‑1 (MQ=255)
       gATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGc                                                                                                                                               >  1:5304342/1‑150 (MQ=255)
        aTTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGTGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCa                                                                                                                                              <  2:446131/150‑1 (MQ=255)
          tttCGCGTCGGTTGGTTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGATGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGTTGGATATTTTCGGGTCGGGCACTTTCACGGAAACGACCGCAAt                                                                                                                                            <  2:755405/150‑1 (MQ=255)
           ttCGCGTCGGTTGGGTTTTACAGCAGGTATTATGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTAACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCACTc                                                                                                                                           >  1:4727794/1‑150 (MQ=255)
             cgcgTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCACAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAg                                                                                                                                         >  1:2077564/1‑150 (MQ=255)
              gcgTCGGTTGTGTTTTCCAGCAGTTATTCGGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGTTTTCACCTCAGAAGAAACCATTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGAACTTTCACGTAAACGACCGCAATCAgg                                                                                                                                        <  2:639616/150‑1 (MQ=255)
              gcgTCGGTTGGGTTTTCCAGCAGGTATTCGGCCAGCAGTTGGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGACGAAACCAGTTTGTCTTGGGTCGGGGAGGAGATTTTGGGGTCCGGCACTTTCACGGACACGACCGCAATCAgg                                                                                                                                        <  2:1011596/150‑1 (MQ=255)
                 tCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCt                                                                                                                                     >  1:3021143/1‑150 (MQ=255)
                  cGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCtt                                                                                                                                    >  2:2831736/1‑150 (MQ=255)
                     ttGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCAc                                                                                                                                 >  2:1842729/1‑150 (MQ=255)
                           tttCCAGCAGGTATTCTGCCAGCTGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCATAAGAAACCAGTTTGTCTTTGGTCTGGGAGTATAATTTCGGGTCCGGAACTTTCACGTAAACGACCGCAATCAGGCCTTCACGCGCAt                                                                                                                           <  2:557916/150‑1 (MQ=255)
                              ccAGCCGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCACCCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGt                                                                                                                        <  2:400984/150‑1 (MQ=255)
                                  cagGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCAcc                                                                                                                    >  2:2633877/1‑150 (MQ=255)
                                  cagGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCAcc                                                                                                                    >  1:1079613/1‑150 (MQ=255)
                                   agGTATTCTGCCCGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCCGAATAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTGGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCg                                                                                                                   <  1:5006744/150‑1 (MQ=255)
                                   agGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCg                                                                                                                   >  2:72145/1‑150 (MQ=255)
                                    ggTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGACGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTGCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCgg                                                                                                                  <  2:1371563/150‑1 (MQ=255)
                                     gTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGt                                                                                                                 >  1:634355/1‑150 (MQ=255)
                                       aTTCTGCCAGAAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTgg                                                                                                               <  2:2743186/150‑1 (MQ=255)
                                        ttCTGCCAGCAGTTCGTTCATCTGCTGTTCACCCGCCTTTTTCACCTCATAATAAACCTGTTTGTCTTTGGTCTGGGAGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGgc                                                                                                              <  2:4718540/150‑1 (MQ=255)
                                           tGCCAGCAGGTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCCGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTACACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCt                                                                                                           <  2:3106585/150‑1 (MQ=255)
                                            gCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTg                                                                                                          >  2:3487566/1‑150 (MQ=255)
                                              cagcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGAc                                                                                                        >  1:5113255/1‑150 (MQ=255)
                                              cagcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGAc                                                                                                        <  2:5506814/150‑1 (MQ=255)
                                               agcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACt                                                                                                       >  1:4066214/1‑150 (MQ=255)
                                                 cagTTCGTTCATCTGCTGTTCAAACGCCGATTTCACAACAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTACGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGAGGCGCTGACttt                                                                                                     >  2:303137/1‑150 (MQ=255)
                                                 acgttctttcTTCTGGTGTAAACCCGCCTATTTCACCTCAGAACAAACCTTTTGTTCTTTGGTCTGGGTGTATATTTTCGGGTCCGGCAATTTCACGTAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACttt                                                                                                     <  1:4794626/148‑1 (MQ=255)
                                                  agTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACGTTg                                                                                                    >  1:2423016/1‑150 (MQ=255)
                                                   gttcgttcATCTGCTTTTCACCCGCCTATTTCACCTCAGAATAAACCATTTTGTCTTTGGTCTGGGAGGATAATTTCGGGGCCGGCAATTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTgg                                                                                                   <  1:2705471/150‑1 (MQ=255)
                                                      cgttcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtt                                                                                                >  2:2323520/1‑150 (MQ=255)
                                                         tcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCttttt                                                                                             >  2:5207009/1‑150 (MQ=255)
                                                          cATCTGCTGTTCACCCGCCGATTTCACCTCCGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtttttt                                                                                            <  2:5346483/150‑1 (MQ=255)
                                                          cATCTGCTGTTCAACCGCCTATTTCACCTCAGAAGAAACCAGTTTGTCTTGGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtttttt                                                                                            <  1:2167108/150‑1 (MQ=255)
                                                           aTCTGCTGTACACCCGCCTATTTCACCTCATAAGAAACCAGTTTGTCTTGGGTCTGGGTGTAGAATTTCGGGTCCGGAACGTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTg                                                                                           <  1:3440627/150‑1 (MQ=255)
                                                            tCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGc                                                                                          >  1:5278439/1‑150 (MQ=255)
                                                             ctggtgTACACCCGCCTATTCCCCCTCAGAAGAACCAATTTTTTCTTGGGTCTGGGAGTATATTTGGGGGTCCGCCACTTCCCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCt                                                                                         <  1:3781653/150‑1 (MQ=255)
                                                             ctgctgTTCAACCGCCGATTTCACCACAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGTAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCt                                                                                         <  1:922448/150‑1 (MQ=255)
                                                                ctgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTa                                                                                      <  2:431907/150‑1 (MQ=255)
                                                                 tgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGATAATTTCGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAg                                                                                     <  2:166334/150‑1 (MQ=255)
                                                                   ttCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGcc                                                                                   >  2:1466761/1‑150 (MQ=255)
                                                                       aCCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttct                                                                               <  2:1126010/150‑1 (MQ=255)
                                                                        ccgccgATTTCACCTCATAATAAACCTTTTTGTATTTGGTCTGGGTGTTTAATTTCGGGTCCGGCAATTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttctt                                                                              <  1:4564603/150‑1 (MQ=255)
                                                                         cgcctATGTCACCTCCTAAGAAACCAGTTTGTCTTTGGTCTGGGTGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                             <  1:3083351/150‑1 (MQ=255)
                                                                         cgccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTGGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                             <  1:3486495/150‑1 (MQ=255)
                                                                         cgccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                             >  1:3541927/1‑150 (MQ=255)
                                                                         cgccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                             <  2:5436914/150‑1 (MQ=255)
                                                                           cctaTTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGATTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGt                                                                           <  1:5436977/147‑1 (MQ=255)
                                                                           ccgATTTCACCTCAGAATAAACCATTTTGTCTTGGGTCTGGGTGGAGAATTTGGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGt                                                                           <  1:3937287/150‑1 (MQ=255)
                                                                            cgttttCACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGATATTGTGGGGTCGGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTc                                                                          <  1:4477359/147‑1 (MQ=255)
                                                                             gATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTcc                                                                         <  2:5471748/150‑1 (MQ=255)
                                                                                  cACCTCAGAAGAAACCAGTTTGTCTTTGGTCGGGGAGGAGATTTTCGGGTCCGGCACTTACACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                    <  1:1524133/150‑1 (MQ=255)
                                                                                    ccTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAgg                                                                  >  2:790397/1‑150 (MQ=255)
                                                                                     cTCAGAATAAACCAGTTTTTCTTTGGTGTGGGTGGATATTTTCGGGTCCGGCACTTACCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGc                                                                 <  1:3293484/150‑1 (MQ=255)
                                                                                      tCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCg                                                                >  1:3715805/1‑150 (MQ=255)
                                                                                       cAGAAGAAACCTGTTTGTCTTTGGTCTGGGTGGATAATTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGt                                                               <  1:1705298/150‑1 (MQ=255)
                                                                                        cgaagaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGtt                                                              <  1:4976601/149‑1 (MQ=255)
                                                                                         gaagaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTc                                                             <  2:3969662/150‑1 (MQ=255)
                                                                                          aagaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCa                                                            >  2:4204078/1‑150 (MQ=255)
                                                                                          aagaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCa                                                            >  2:3454246/1‑150 (MQ=255)
                                                                                          aagaaACCAGTTTGTCTTGGGTCTGGGAGGATAATTTCGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCa                                                            <  2:5083054/150‑1 (MQ=255)
                                                                                                  aGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACgg                                                    >  1:1924675/1‑150 (MQ=255)
                                                                                                   gTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAggg                                                         >  2:3649510/1‑144 (MQ=255)
                                                                                                      tGTCTTTGGTCGGGGAGGAGAATTTCGGGTCCGGCACTTTCACGTAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCa                                                <  2:2528020/150‑1 (MQ=255)
                                                                                                         cTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATcg                                             >  1:5372912/1‑150 (MQ=255)
                                                                                                               tCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCAc                                       >  2:3282358/1‑150 (MQ=255)
                                                                                                                 tGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACgg                                     >  1:1344823/1‑150 (MQ=255)
                                                                                                                 tGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACgg                                     >  1:3721706/1‑150 (MQ=255)
                                                                                                                   ggaggaGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGaa                                   >  1:5040792/1‑150 (MQ=255)
                                                                                                                     aggagAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGc                                 >  2:1420484/1‑150 (MQ=255)
                                                                                                                       gagaTTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCt                               <  2:561918/150‑1 (MQ=255)
                                                                                                                       gagaATTTCGGGTCCGGCCCTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCt                               <  2:4242238/150‑1 (MQ=255)
                                                                                                                       gagaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCt                               <  2:1476248/150‑1 (MQ=255)
                                                                                                                        agaTTTTCGGGTCCGGCAATTTCACGGAAACGACCGCAATCAGGACTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                              <  1:3876637/150‑1 (MQ=255)
                                                                                                                        agaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                              >  1:1495500/1‑150 (MQ=255)
                                                                                                                        agaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                              >  1:3598467/1‑150 (MQ=255)
                                                                                                                        agaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                              >  1:832496/1‑150 (MQ=255)
                                                                                                                         gaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTTGCGCTGACTTTGGCTTTTTTGCTGTAGCCATCTTTGTCCATGTAGGCGATCAGGGTACGGGTCATCGCCGCACGGAAGCCTGc                             >  2:53724/1‑150 (MQ=255)
                                                                                                                           aTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCa                           >  2:2237068/1‑150 (MQ=255)
                                                                                                                            tttCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAg                          <  1:3209885/150‑1 (MQ=255)
                                                                                                                             ttCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAgg                         <  1:4079340/150‑1 (MQ=255)
                                                                                                                              tCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGt                        <  2:4430541/150‑1 (MQ=255)
                                                                                                                               cGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTg                       >  2:2064128/1‑150 (MQ=255)
                                                                                                                                 ggTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAg                     >  2:5480272/1‑150 (MQ=255)
                                                                                                                                  gTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGt                    >  2:1202489/1‑150 (MQ=255)
                                                                                                                                  gTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGt                    <  2:4207431/150‑1 (MQ=255)
                                                                                                                                    ccGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAc                  <  2:2961356/150‑1 (MQ=255)
                                                                                                                                     cGGCACTTACACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAcc                 <  1:1644064/150‑1 (MQ=255)
                                                                                                                                      ggCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccg                >  2:3051686/1‑150 (MQ=255)
                                                                                                                                      ggCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccg                >  2:4870236/1‑150 (MQ=255)
                                                                                                                                        cACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCGGCCAGGTGAGTAccgcc              >  1:3361070/1‑150 (MQ=255)
                                                                                                                                         aCTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccgccg             <  1:2595065/150‑1 (MQ=255)
                                                                                                                                                cGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCt      >  1:3393067/1‑150 (MQ=255)
                                                                                                                                                cGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCt      <  2:2461358/150‑1 (MQ=255)
                                                                                                                                                    aaCGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCgg  >  1:3834455/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGG  >  CP009273/3872363‑3872660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑