Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	4,362,446	+GGCGGTATG	11.2%	coding (1605/1647 nt)	groL →	Cpn60 chaperonin GroEL, large subunit of GroESL

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4362446	180 (1.070)	23 (0.130) +GGCGGTATG	4/228	NT	11.2%	coding (1605/1647 nt)	groL	Cpn60 chaperonin GroEL, large subunit of GroESL
	?	CP009273	4362447 =	190 (1.010)					coding (1606/1647 nt)	groL	Cpn60 chaperonin GroEL, large subunit of GroESL

ACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/4362301‑4362446 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAA  >  CP009273/4362447‑4362570                                                                                                                                                   |||||||||                                                                                                                             ACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGG                                                                                                                                     >  1:1256931/1‑148    TGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGG                                                                                                                                  >  2:802190/1‑148     GATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGT                                                                                                                                 >  1:2470822/1‑148      ATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTA                                                                                                                                >  2:2425616/1‑148      ATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTA                                                                                                                                <  2:2288207/148‑1      ATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTA                                                                                                                                <  2:1663804/148‑1      ATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTA                                                                                                                                <  2:1663643/148‑1       TCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTAT                                                                                                                               <  2:1224829/148‑1       TCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTAT                                                                                                                               >  2:2251433/1‑148       TCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTAT                                                                                                                               >  2:216754/1‑148       TCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTAT                                                                                                                               >  1:2220871/1‑148       TCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTAT                                                                                                                               >  2:87970/1‑148        CGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATG                                                                                                                              <  2:2595471/148‑1        CGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATG                                                                                                                              <  2:802869/148‑1        CGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATG                                                                                                                              <  2:803725/148‑1        CGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATG                                                                                                                              <  2:811684/148‑1         GACATGGGTCTCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGG                                                                                                                             >  2:1726152/1‑148         GACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGG                                                                                                                             >  2:1726093/1‑148           CATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGC                                                                                                                           >  1:2301979/1‑148                                                         TACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCT                                                                             >  2:2596856/1‑148                                                                                                  CATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCT                                    >  1:35917/1‑148                                                                                                  CATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCT                                    >  1:1715349/1‑148                                                                                                       TTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGG                               >  2:2515038/1‑148                                                                                                                                    GACTTAGGCGCTGCTGGCGGTATGGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAA  >  1:716248/1‑148                                                                                                                                    GACTTAGGCGCTGCTGGCGGTATGGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAA  >  1:3022411/1‑148                                                                                                                                                   |||||||||                                                                                                                             ACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/4362301‑4362446 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCAGAAATGTCTGGGGGTTTTTCTTTTGGTCATCTTTCTAGTATAA  >  CP009273/4362447‑4362570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.