Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 31,710 C→A 88.0% I298I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7100CA88.0% 347.0 / 41.7 153I298I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (11/8);  new base A (73/61);  total (84/69)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

CGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCC  >  CP009273/31566‑31854
                                                                                                                                                |                                                                                                                                                
cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaa                                                                                                                                               <  2:2666847/148‑1 (MQ=255)
 gcgcCAGCCCAAACGCTGACCGACAAATAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaaa                                                                                                                                              <  1:1399156/148‑1 (MQ=255)
 gcgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaaa                                                                                                                                              <  1:731801/148‑1 (MQ=255)
 gcgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaaa                                                                                                                                              <  1:731480/148‑1 (MQ=255)
 gcgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaaa                                                                                                                                              <  1:1382074/148‑1 (MQ=255)
  cgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAAt                                                                                                                                             <  1:2322402/148‑1 (MQ=255)
      aGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAc                                                                                                                                         >  2:879981/1‑148 (MQ=255)
        cccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAccc                                                                                                                                       >  2:2467720/1‑148 (MQ=255)
           aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                    >  1:1187387/1‑148 (MQ=255)
           aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                    <  1:444015/148‑1 (MQ=255)
           aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                    >  2:2683916/1‑148 (MQ=255)
             aCGCTGACCGACAACGAATATCAAATCATGCGTAACGCCTCGAGGGCGGTGCTGCGTGAAATCGGCGTTGAATCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                  <  2:2766102/148‑1 (MQ=255)
                cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                               <  2:633983/148‑1 (MQ=255)
                cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                               <  1:528839/148‑1 (MQ=255)
                 tGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTc                                                                                                                              <  1:378725/148‑1 (MQ=255)
                  gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTcc                                                                                                                             >  1:1881330/1‑148 (MQ=255)
                   aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTccc                                                                                                                            >  2:2223006/1‑148 (MQ=255)
                      gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGtt                                                                                                                         <  2:454317/148‑1 (MQ=255)
                        cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                       >  1:686920/1‑148 (MQ=255)
                           aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                    >  1:3086009/1‑148 (MQ=255)
                           aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                    >  1:3086026/1‑148 (MQ=255)
                            gAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                                                   <  2:2911942/148‑1 (MQ=255)
                            gAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                                                   >  2:847734/1‑148 (MQ=255)
                              atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGTTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcg                                                                                                                 <  1:897474/148‑1 (MQ=255)
                              atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcg                                                                                                                 <  1:2107322/148‑1 (MQ=255)
                                 tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTg                                                                                                              >  2:2405237/1‑148 (MQ=255)
                                  cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                             >  1:1491626/1‑148 (MQ=255)
                                  cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                             >  1:2608758/1‑148 (MQ=255)
                                   aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc                                                                                                            >  1:2573824/1‑148 (MQ=255)
                                       cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                        >  1:2308246/1‑148 (MQ=255)
                                        aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGa                                                                                                       >  2:1735296/1‑148 (MQ=255)
                                        aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGa                                                                                                       >  2:1747435/1‑148 (MQ=255)
                                         tGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaa                                                                                                      <  2:2351203/148‑1 (MQ=255)
                                         tGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaa                                                                                                      >  2:3268958/1‑148 (MQ=255)
                                           cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAg                                                                                                    <  1:1030196/148‑1 (MQ=255)
                                           cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAg                                                                                                    <  1:1048456/148‑1 (MQ=255)
                                              aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                 >  2:1119350/1‑148 (MQ=255)
                                              aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                 >  1:914758/1‑148 (MQ=255)
                                                cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                               >  1:2418315/1‑148 (MQ=255)
                                                cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAGTGTTATAGACATGAACCCACGCGTGTCCCGGTCTTCGGCGCTGGCGTCGAAGGCGAcc                                                                                               >  1:1908384/1‑148 (MQ=255)
                                                   cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                            >  2:926454/1‑148 (MQ=255)
                                                   cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAGTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                            >  2:2543588/1‑148 (MQ=255)
                                                     cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                          >  2:676505/1‑148 (MQ=255)
                                                     cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                          >  2:676185/1‑148 (MQ=255)
                                                       aTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTcc                                                                                        >  2:1031777/1‑148 (MQ=255)
                                                                 tgcGTGAAATCGGCTTTGAAACGGTTGGTTCCACCGTGCTGTTGGCGTTGAACCCGAAAAAGGGTCGTCTGATTGTTATAGAAATGAACCCCCGCGTGGCCCGTTCTGCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTaa                                                                              <  2:1200782/148‑1 (MQ=255)
                                                                   cGTGAAATCGGCGTTGAAACGGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTTATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAg                                                                            <  2:2179245/148‑1 (MQ=255)
                                                                    gTGAAATCGGCGTTGAAACGGTTGGTTCCAACGTTCAGTTTGCGGTGAACCCTAAAAACGGTCGTCTGTTTGTTATAGAAATCAACCCACGCGTGGCCGGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGt                                                                           <  2:763454/148‑1 (MQ=255)
                                                                           cGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTTCGGTGAACCCCAAAAAAGGTCGTCTGATTGTTATAGAAATTAACCCACGCCTGTCCCGTTCTTAGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGgcggcg                                                                    >  1:1503455/1‑148 (MQ=255)
                                                                             gCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGACCCCGAAAAACGGTCGTCTGTTTGTTATCGAAATGAACCCACGGGGGGCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaa                                                                  <  2:2423661/148‑1 (MQ=255)
                                                                             gCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaa                                                                  >  1:3258382/1‑148 (MQ=255)
                                                                             gCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaa                                                                  >  1:3222533/1‑148 (MQ=255)
                                                                              cGTTGAACCGGGTGGTACCAACGTAGTGTTGGGGGTGAACCCGAAAAAGGTTCGTTTGTTTTTTATAGAAATCAACCCCCGCGGGGCCGGTTCTTCGGGGCTGGCGTCTAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                 <  2:560639/148‑1 (MQ=255)
                                                                              cGTTGAAACCGGTGGTTCAAAGGTTCTGTTTGGGGTGAACCCGAAAAACGGTCGTCTGATTTTTATAGAAATGAACCCCCGCGTGTCCCGTTCTTCGGCGCTGGGGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                 <  2:776753/148‑1 (MQ=255)
                                                                               gTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAc                                                                >  1:2015785/1‑148 (MQ=255)
                                                                                   aaaCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGc                                                            >  2:2846833/1‑148 (MQ=255)
                                                                                     cccGGTGGTACCAACGTACATTTTGCGTTCAACCCGAAAAACGGTCGTCTGATTTTTATATAAATGACCCCCCGCGTGGCCGGTTCTGGGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                          <  2:1153444/147‑1 (MQ=255)
                                                                                     aCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                          >  1:2250391/1‑148 (MQ=255)
                                                                                     aCCGGTGGTACAACCGTTCAGTTTGCGGTCACCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                          <  2:2851087/148‑1 (MQ=255)
                                                                                       cGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTg                                                        >  2:3085182/1‑148 (MQ=255)
                                                                                         gtggtgCCACCGTTCAGTGGGCGGTGACCCCCAAAAAGGGACGTCTTATTGTTATCGAAATCACCCCCCGGGGGGCCCGTTGTTCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                      <  2:868964/148‑1 (MQ=255)
                                                                                           ggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGtt                                                    >  1:2481223/1‑148 (MQ=255)
                                                                                             ttCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTac                                                  >  1:196493/1‑148 (MQ=255)
                                                                                             ttCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTac                                                  >  1:196550/1‑148 (MQ=255)
                                                                                              tCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTaca                                                 >  1:2205409/1‑148 (MQ=255)
                                                                                               ccACCGTTCAGTTGGCGGTCAACCCGAAAAACGGTCGTCTTATTTTTATCGAAATGACCCCACGCGTGGCCCGTTCTGCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTacac                                                <  2:1491626/148‑1 (MQ=255)
                                                                                               caaaggTTCAGTTGGCGGTGAACCCCAAAAACGGTCGTGTGATTGTTATCGAAATGACCCCCCGGGGGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTacac                                                <  2:2608758/143‑1 (MQ=255)
                                                                                                cAACGTTCAGTTGGCGGTTAACCCGAAAACCGGTCGTCTTATTGTTATAGAAATGAACCCAGGCGTGGCCCGTTCTTCGGCGCGGGCGCCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAcc                                               <  2:1587075/148‑1 (MQ=255)
                                                                                                 aaCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                              >  1:1831968/1‑148 (MQ=255)
                                                                                                 aaCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                              >  1:1919993/1‑148 (MQ=255)
                                                                                                    tttCTGTTTGCGGTCACCCCCAAAAAGGGTGGGTTGTTTGTTATAAAAATCACCCCCCGCGGGACCGGTTGTGCGGGGGGGGCGACCACCGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcg                                           <  1:1531704/147‑1 (MQ=255)
                                                                                                     ttCTGTTTGCGGTGACCCCGAAAACCGGTCGTCTGTTTGTTATAGAAATGAACCCCCGCGTGACCCGTTCTTCGGCGCTGGCGCCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcga                                          <  2:2461993/148‑1 (MQ=255)
                                                                                                     ttCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCCCGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcga                                          <  2:2701973/148‑1 (MQ=255)
                                                                                                         gTTTGCGGTGAACCCGAAAAACGGTCGTCTGTTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa                                      >  1:89108/1‑148 (MQ=255)
                                                                                                         gTTTGCGGTCAACCCCAAAACGGGTCGTCTTTTTTTAATACAAACCACCCCCCGCGGGGCCGGTCCGGCGGCGCTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa                                      <  1:188950/148‑1 (MQ=255)
                                                                                                             gCGGTGAACCCGAAAAACGGTGGTCTTTTTTTTATATAAATGAACCCACGCGTGTCCCGTTCTTCGGCGGTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtga                                  <  2:2573824/148‑1 (MQ=255)
                                                                                                                 tGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                              >  1:1624279/1‑148 (MQ=255)
                                                                                                                 tGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                              >  1:1624100/1‑148 (MQ=255)
                                                                                                                    aCCCTAAAAAGGGTCTTCTTTTTTTTATAGAAATCACCCCCCGGGGGGCCGGTTCTGGGGGGGTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                           <  1:1031777/148‑1 (MQ=255)
                                                                                                                    aCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                           >  1:3269995/1‑148 (MQ=255)
                                                                                                                    aCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                           >  1:1000683/1‑148 (MQ=255)
                                                                                                                         aaaacCGGTCGTCTGATTGTTATAGAAATGAACCCCCGCGGGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCa                      <  2:786781/148‑1 (MQ=255)
                                                                                                                         aaaaaCGGTCGTCTGATTGTTATATAAATGACCCCCCGCGTGTCCCGTTATTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCa                      <  2:1856734/148‑1 (MQ=255)
                                                                                                                          aaaaGGGTCGTCTGATTGTTATATAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCAc                     <  1:1925666/148‑1 (MQ=255)
                                                                                                                           aaaGGGTCGTCTGATTTTTATCCAAATGACCCCCCGCGTGCCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                    <  1:2467720/148‑1 (MQ=255)
                                                                                                                           aaaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                    >  2:1065610/1‑148 (MQ=255)
                                                                                                                           aaaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                    >  1:1226243/1‑148 (MQ=255)
                                                                                                                              cGGTCGTCTGATTGTTATAGAAAAGAAACCAAGCGTTTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAATGATGAACGACATCACTGGc                 >  2:1120256/1‑148 (MQ=255)
                                                                                                                                ttcgtcTTTTTTTTTTAGAAATCAACCCCCGGGGGGCCGGTTGTTCGGCGCGGGCGCCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCgg               <  1:1735296/147‑1 (MQ=255)
                                                                                                                                gtcgtcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCgg               >  1:2257197/1‑148 (MQ=255)
                                                                                                                                  cgtcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc             >  2:1573721/1‑148 (MQ=255)
                                                                                                                                  cgtcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc             >  2:817871/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt           <  2:332814/148‑1 (MQ=255)
                                                                                                                                     ttttttGTTATACAAATCACCCCCCGGGGGGCCGGTTCTGGGGCGGGGGCGACGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa          <  1:356035/144‑1 (MQ=255)
                                                                                                                                       gATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACt        >  2:474065/1‑148 (MQ=255)
                                                                                                                                        aTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc       >  1:3189118/1‑148 (MQ=255)
                                                                                                                                            ttATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc   >  2:1722565/1‑148 (MQ=255)
                                                                                                                                             tataGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc  >  1:2801723/1‑148 (MQ=255)
                                                                                                                                             tataGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc  >  2:3021268/1‑148 (MQ=255)
                                                                                                                                             tataGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc  >  1:2801894/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCC  >  CP009273/31566‑31854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑