Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,893,446 T→A 92.0% K63* (AAA→TAA)  ygcW ← putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,893,4460TA92.0% 686.1 / 39.5 226K63* (AAA→TAA) ygcWputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (10/8);  new base A (113/95);  total (123/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 227 total aligned reads displayed.

AGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGA  >  CP009273/2893301‑2893590
                                                                                                                                                 |                                                                                                                                                
atcACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGttttt                                                                                                                                                <  2:1995783/146‑1 (MQ=255)
 gCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTc                                                                                                                                               >  1:2094240/1‑148 (MQ=255)
 gCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTc                                                                                                                                               >  1:429402/1‑148 (MQ=255)
  cACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCa                                                                                                                                              <  1:2611783/148‑1 (MQ=255)
    ccTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAAt                                                                                                                                            <  1:2388130/148‑1 (MQ=255)
    ccTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAAt                                                                                                                                            >  1:1918361/1‑148 (MQ=255)
     cgtATTCAGCTTACAAAGACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATc                                                                                                                                           <  2:2202870/146‑1 (MQ=255)
     cTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAAta                                                                                                                                           <  1:384735/148‑2 (MQ=255)
       tATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAt                                                                                                                                         <  2:1733786/148‑1 (MQ=255)
        aTTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAtt                                                                                                                                        <  2:2071245/148‑1 (MQ=255)
         ttCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAttt                                                                                                                                       >  2:898143/1‑148 (MQ=255)
         ttCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAttt                                                                                                                                       >  2:3093555/1‑148 (MQ=255)
         ttCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAttt                                                                                                                                       >  2:774832/1‑148 (MQ=255)
         ttCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCAttt                                                                                                                                       >  2:3093604/1‑148 (MQ=255)
          tCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTc                                                                                                                                      >  2:1624864/1‑148 (MQ=255)
          tCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTc                                                                                                                                      >  2:1210197/1‑148 (MQ=255)
          tCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTc                                                                                                                                      >  2:879676/1‑148 (MQ=255)
            aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCt                                                                                                                                    <  1:1651132/148‑1 (MQ=255)
            aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCt                                                                                                                                    <  2:2723000/148‑1 (MQ=255)
            aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCt                                                                                                                                    <  1:2502622/148‑1 (MQ=255)
            aGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCt                                                                                                                                    >  1:2216690/1‑148 (MQ=255)
            aGCTTACAAATACCGGCAGTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCt                                                                                                                                    <  1:1482591/148‑1 (MQ=255)
              cTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCttt                                                                                                                                  >  2:2897746/1‑148 (MQ=255)
               ttACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTg                                                                                                                                 >  2:688977/1‑148 (MQ=255)
                  cAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGttt                                                                                                                              >  1:799211/1‑148 (MQ=255)
                   aaaTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTc                                                                                                                             >  1:1698611/1‑148 (MQ=255)
                   aaaTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTc                                                                                                                             >  2:384845/1‑148 (MQ=255)
                     aTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGc                                                                                                                           <  2:2649508/148‑1 (MQ=255)
                     aTACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGc                                                                                                                           <  1:1253534/148‑1 (MQ=255)
                      tACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGcc                                                                                                                          >  1:2805299/1‑148 (MQ=255)
                       aCCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCg                                                                                                                         >  2:2985973/1‑148 (MQ=255)
                       aCCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCg                                                                                                                         >  2:460986/1‑148 (MQ=255)
                        ccGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGt                                                                                                                        <  1:1316061/148‑1 (MQ=255)
                         cGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGtt                                                                                                                       <  2:1889299/148‑1 (MQ=255)
                         cGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGtt                                                                                                                       <  2:576787/148‑1 (MQ=255)
                           gCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTAt                                                                                                                     <  1:2000804/148‑1 (MQ=255)
                              ttGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCtt                                                                                                                  >  2:1906394/1‑148 (MQ=255)
                                gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTg                                                                                                                <  1:588940/148‑1 (MQ=255)
                                gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTg                                                                                                                <  1:1522052/148‑1 (MQ=255)
                                gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTg                                                                                                                <  2:793523/148‑1 (MQ=255)
                                 ttAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGa                                                                                                               <  1:2748985/148‑1 (MQ=255)
                                  tAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGAc                                                                                                              >  2:1228522/1‑148 (MQ=255)
                                     ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTgaagaa                                                                                                           >  1:2161725/1‑148 (MQ=255)
                                     ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaa                                                                                                           >  2:1711113/1‑148 (MQ=255)
                                     ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaa                                                                                                           >  2:34780/1‑148 (MQ=255)
                                      cAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaaa                                                                                                          <  1:819852/148‑1 (MQ=255)
                                      cAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaaa                                                                                                          <  2:259038/148‑1 (MQ=255)
                                                  tGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAg                                                                                              >  1:2290367/1‑148 (MQ=255)
                                                    tACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGat                                                                                            <  2:3229719/148‑1 (MQ=255)
                                                     aCCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGata                                                                                           <  1:2554129/148‑1 (MQ=255)
                                                       cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATAtt                                                                                         <  2:1831011/148‑1 (MQ=255)
                                                         aaaCGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                                       <  2:1449414/148‑1 (MQ=255)
                                                           aCGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgcg                                                                                     >  1:3147781/1‑148 (MQ=255)
                                                            cGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgcgc                                                                                    <  1:2418214/148‑1 (MQ=255)
                                                            cGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgcgc                                                                                    <  1:758469/148‑1 (MQ=255)
                                                                cacaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGc                                                                                >  1:1417536/1‑148 (MQ=255)
                                                                 acaGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCt                                                                               >  2:540197/1‑148 (MQ=255)
                                                                  caGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTTTCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCtt                                                                              <  2:1934102/148‑1 (MQ=255)
                                                                    gcaagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTg                                                                            >  1:54064/1‑148 (MQ=255)
                                                                    gcaagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACAAGGAATAAAGATATTTGCGCCAGCTTTg                                                                            >  1:3093473/1‑148 (MQ=255)
                                                                     caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                           >  1:1805612/1‑148 (MQ=255)
                                                                     caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                           <  1:1157010/148‑1 (MQ=255)
                                                                     caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                           >  1:1259486/1‑148 (MQ=255)
                                                                     caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                           >  2:938089/1‑148 (MQ=255)
                                                                     caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                                           >  2:1545001/1‑148 (MQ=255)
                                                                         cagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCaa                                                                       >  1:1001100/1‑148 (MQ=255)
                                                                            cGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGc                                                                    >  2:127823/1‑148 (MQ=255)
                                                                              aTAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCa                                                                  <  1:2872560/148‑1 (MQ=255)
                                                                               tAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCAt                                                                 >  1:2753298/1‑148 (MQ=255)
                                                                                aaTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATg                                                                <  2:2761370/148‑1 (MQ=255)
                                                                                aaTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATg                                                                >  2:1048171/1‑148 (MQ=255)
                                                                                aaTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATg                                                                >  2:229822/1‑148 (MQ=255)
                                                                                 aTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATgg                                                               >  1:1065746/1‑148 (MQ=255)
                                                                                  tcttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGc                                                              >  2:1102011/1‑148 (MQ=255)
                                                                                     tctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCaaa                                                           >  1:2950535/1‑148 (MQ=255)
                                                                                        gcggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGc                                                        <  2:3171133/148‑1 (MQ=255)
                                                                                        gcggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGc                                                        <  1:2605873/148‑1 (MQ=255)
                                                                                          ggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCt                                                      <  1:460986/148‑1 (MQ=255)
                                                                                               gcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCt                                                 <  2:1255554/148‑1 (MQ=255)
                                                                                                          tGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTAtt                                      <  2:2669204/148‑1 (MQ=255)
                                                                                                            aTACCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTcc                                    >  2:2225473/1‑148 (MQ=255)
                                                                                                              aCCCACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCa                                  >  2:2871142/1‑148 (MQ=255)
                                                                                                                 cACCTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCg                               >  1:3208482/1‑148 (MQ=255)
                                                                                                                   ccTGCATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGt                             <  1:1876830/148‑1 (MQ=255)
                                                                                                                       cATGAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACa                         >  1:1567042/1‑148 (MQ=255)
                                                                                                                         tGAAGGCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAt                       <  2:2104746/148‑1 (MQ=255)
                                                                                                                          gAAGTCCACCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAtt                      >  2:1050859/1‑148 (MQ=255)
                                                                                                                              tctaCCTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCgg                  >  1:1347785/4‑148 (MQ=255)
                                                                                                                                  ccTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGtttt              >  1:712701/1‑148 (MQ=255)
                                                                                                                                  ccTCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGtttt              <  1:3311948/148‑1 (MQ=255)
                                                                                                                                   cTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTa             >  1:2600237/1‑148 (MQ=255)
                                                                                                                                    tCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc            >  2:861007/1‑148 (MQ=255)
                                                                                                                                    tCAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc            >  2:144508/1‑148 (MQ=255)
                                                                                                                                     cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAcc           >  2:637096/1‑148 (MQ=255)
                                                                                                                                     cAACACCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAcc           <  2:1686611/148‑1 (MQ=255)
                                                                                                                                       acacCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCtt         <  1:1620836/148‑1 (MQ=255)
                                                                                                                                       acacCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCtt         <  1:127823/148‑1 (MQ=255)
                                                                                                                                         acCCTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTc       >  1:3110582/1‑148 (MQ=255)
                                                                                                                                            cTGTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAgg    >  2:2543047/1‑148 (MQ=255)
                                                                                                                                              gTTATTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGga  >  2:365621/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGA  >  CP009273/2893301‑2893590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑