Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,902,302 C→A 29.9% D242Y (GAT→TAT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,902,3020CA29.9% 365.1 / 140.0 194D242Y (GAT→TAT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (66/70);  new base A (23/35);  total (89/105)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 197 total aligned reads displayed.

GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTG  >  CP009273/2902156‑2902449
                                                                                                                                                  |                                                                                                                                                   
gACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCg                                                                                                                                                    <  1:2072953/148‑1 (MQ=255)
 aCTTCACTTACCGTTTTCGATTCTTCGAAGATAACCTGTTCCCATTCGGACAGTTTCGCTTCCGGGCAGTTTAATCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGa                                                                                                                                                   <  1:3029871/148‑1 (MQ=255)
    tCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGt                                                                                                                                                <  1:22723/148‑1 (MQ=255)
       cTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCtt                                                                                                                                             >  2:705216/1‑148 (MQ=255)
       cTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCtt                                                                                                                                             >  1:54624/1‑148 (MQ=255)
        ttACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCttt                                                                                                                                            >  1:2154916/1‑148 (MQ=255)
        ttACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCttt                                                                                                                                            <  1:199275/148‑1 (MQ=255)
         tACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTc                                                                                                                                           >  1:127951/1‑148 (MQ=255)
         tACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTc                                                                                                                                           <  2:735557/148‑1 (MQ=255)
         tACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTc                                                                                                                                           >  1:446131/1‑148 (MQ=255)
               gTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATACTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGaa                                                                                                                                     <  1:1411976/148‑1 (MQ=255)
               gTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGaa                                                                                                                                     <  1:1859292/148‑1 (MQ=255)
                ttCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGaaa                                                                                                                                    <  1:236873/148‑1 (MQ=255)
                    cttcttCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAc                                                                                                                                >  1:523891/1‑148 (MQ=255)
                     ttcttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAcc                                                                                                                               >  1:892768/1‑148 (MQ=255)
                       cttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGc                                                                                                                             <  1:275912/148‑1 (MQ=255)
                       cttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGc                                                                                                                             <  1:1684727/148‑1 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCACCAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                           >  2:2860576/1‑148 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                           >  1:2820085/1‑148 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                           <  1:1252849/148‑1 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                           >  2:3031119/1‑148 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                           <  1:466764/148‑1 (MQ=255)
                          cGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCttt                                                                                                                          <  2:1135569/148‑1 (MQ=255)
                            aaGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCttttt                                                                                                                        <  1:1365570/148‑1 (MQ=255)
                            aaGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCttttt                                                                                                                        >  2:226186/1‑148 (MQ=255)
                                  aCCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGaa                                                                                                                  <  2:2781264/148‑1 (MQ=255)
                                  aCCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGaa                                                                                                                  >  2:42941/1‑148 (MQ=255)
                                   ccTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATATATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAAc                                                                                                                 >  1:2771781/1‑148 (MQ=255)
                                    cTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACa                                                                                                                >  2:810491/1‑148 (MQ=255)
                                     tGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt                                                                                                               <  1:1668442/148‑1 (MQ=255)
                                       ttCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTa                                                                                                             >  2:2668103/1‑148 (MQ=255)
                                         cccATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACa                                                                                                           >  1:2851504/1‑148 (MQ=255)
                                           cATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAGCAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGa                                                                                                         <  1:2078303/148‑1 (MQ=255)
                                           cATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGa                                                                                                         <  1:442027/148‑1 (MQ=255)
                                           cATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATAGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATAGACGTCTTTAAGAGAAATAACCGCTTTTTCCGGAACATTACAGa                                                                                                         >  1:850448/1‑148 (MQ=255)
                                              tCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACa                                                                                                      <  2:119306/148‑1 (MQ=255)
                                                ggACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAAt                                                                                                    <  1:2338949/148‑1 (MQ=255)
                                                      aTTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATATATGGAATCGACGTCTTTCAGAGAAATAAACCCTTTTTACGGAACATTACAGAAAAAAGCAATc                                                                                              >  1:1265124/1‑148 (MQ=255)
                                                      aTTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATc                                                                                              >  2:2332226/1‑148 (MQ=255)
                                                      aTTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATc                                                                                              >  1:1925689/1‑148 (MQ=255)
                                                        tCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCACCAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCtt                                                                                            >  2:2734946/1‑148 (MQ=255)
                                                           cTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGc                                                                                         <  2:2977633/148‑1 (MQ=255)
                                                              ccGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcg                                                                                      <  2:1781018/148‑1 (MQ=255)
                                                                  gCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgttcg                                                                                  <  1:1492285/148‑1 (MQ=255)
                                                                      tttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGttcgttcg                                                                              >  1:2414435/1‑148 (MQ=255)
                                                                       ttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGc                                                                             >  1:1876006/1‑148 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAg                                                                     <  1:2172047/148‑1 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAg                                                                     <  2:2157125/148‑1 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAg                                                                     >  1:570545/1‑148 (MQ=255)
                                                                                aTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTGCGCCGGAACAgc                                                                    >  2:1886860/1‑148 (MQ=255)
                                                                                aTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgc                                                                    >  2:2923514/1‑148 (MQ=255)
                                                                                   gTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGcgcg                                                                 <  1:1027539/148‑1 (MQ=255)
                                                                                    tttACAAATATAATCGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGa                                                                >  1:100591/1‑148 (MQ=255)
                                                                                    tttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGa                                                                >  1:1079988/1‑148 (MQ=255)
                                                                                     ttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGAt                                                               >  2:1871232/1‑148 (MQ=255)
                                                                                       aCAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCt                                                             <  1:1067011/148‑1 (MQ=255)
                                                                                       aCAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCt                                                             <  1:1093850/148‑1 (MQ=255)
                                                                                         aaaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGa                                                           >  2:1718730/1‑148 (MQ=255)
                                                                                            tataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACg                                                        >  1:1600403/1‑148 (MQ=255)
                                                                                            tataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACg                                                        >  2:56727/1‑148 (MQ=255)
                                                                                             ataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGa                                                       <  2:3234240/148‑1 (MQ=255)
                                                                                              taATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGAc                                                      >  2:3013006/1‑148 (MQ=255)
                                                                                               aaTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACa                                                     >  2:1720353/1‑148 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                    <  1:2406129/148‑1 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                    >  2:1734034/1‑148 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                    <  2:2083588/148‑1 (MQ=255)
                                                                                                      cAGCCCCTTAGATTTCAACAGGCCCTGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAgg                                              <  2:1312770/148‑1 (MQ=255)
                                                                                                      cAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAgg                                              <  1:3305436/148‑1 (MQ=255)
                                                                                                      cAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAgg                                              <  2:523658/148‑1 (MQ=255)
                                                                                                        gCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGAt                                            <  2:1361342/148‑1 (MQ=255)
                                                                                                        gCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGAt                                            >  2:2814271/1‑148 (MQ=255)
                                                                                                         ccccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATg                                           >  2:1645788/1‑148 (MQ=255)
                                                                                                         ccccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATg                                           <  2:756102/148‑1 (MQ=255)
                                                                                                          cccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGt                                          <  2:159740/148‑1 (MQ=255)
                                                                                                              gagaTTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAgg                                      <  2:368056/148‑1 (MQ=255)
                                                                                                              gagaTTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAgg                                      <  2:1105759/148‑1 (MQ=255)
                                                                                                                  tttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTgg                                  <  2:2414435/148‑1 (MQ=255)
                                                                                                                  tttCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTgg                                  <  2:1226801/148‑1 (MQ=255)
                                                                                                                   ttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                 <  1:2734946/148‑1 (MQ=255)
                                                                                                                   ttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                 <  1:3197510/148‑1 (MQ=255)
                                                                                                                     cAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATa                               <  1:819139/148‑1 (MQ=255)
                                                                                                                     cAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATa                               >  1:1719215/1‑148 (MQ=255)
                                                                                                                     cAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATa                               <  2:1468615/148‑1 (MQ=255)
                                                                                                                     cAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTATTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATa                               <  2:1091525/148‑1 (MQ=255)
                                                                                                                       aCAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATAcc                             >  1:2938208/1‑148 (MQ=255)
                                                                                                                         aGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGa                           <  1:988192/148‑1 (MQ=255)
                                                                                                                         aGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGa                           <  1:1893121/148‑1 (MQ=255)
                                                                                                                          ggCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGAt                          >  2:2354522/1‑148 (MQ=255)
                                                                                                                           gCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATg                         >  1:2691117/1‑148 (MQ=255)
                                                                                                                              cGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGgag                      >  1:1814866/1‑148 (MQ=255)
                                                                                                                                gAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGagag                    <  2:3159114/148‑1 (MQ=255)
                                                                                                                                       ataGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGctct             <  1:2596260/148‑1 (MQ=255)
                                                                                                                                         aGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCttt           >  1:1355381/1‑148 (MQ=255)
                                                                                                                                          gATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGAAGCTCtttt          >  1:3031252/1‑148 (MQ=255)
                                                                                                                                            tGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTAc        >  2:1961105/1‑148 (MQ=255)
                                                                                                                                             ggAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACa       <  2:1850044/148‑1 (MQ=255)
                                                                                                                                             ggAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATATGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACa       >  1:3135638/1‑148 (MQ=255)
                                                                                                                                              gAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACag      <  1:3259629/148‑1 (MQ=255)
                                                                                                                                                 tCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGt   >  1:1840547/1‑148 (MQ=255)
                                                                                                                                                  cGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTg  >  2:2627001/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTG  >  CP009273/2902156‑2902449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑