BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,983,568	C→A	7.2%	R51I (AGA→ATA)	yqeK ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,983,568	0	C	A	7.2%	825.9 / 26.4	235	R51I (AGA→ATA)	yqeK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (103/115); new base A (8/9); total (111/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

Only 100 of 240 total aligned reads displayed.

TCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGT  >  CP009273/2983421‑2983715

tCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATc                                                                                                                                                     <  1:1064490/148‑1 (MQ=255)

 cAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCt                                                                                                                                                    <  2:1532635/148‑1 (MQ=255)

 cAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCt                                                                                                                                                    >  1:1447742/1‑148 (MQ=255)

      tagGAAAGTACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAAATCATAATAGACAGATCGTGAGATATAACTGTTTTTAACTTAACTCTTTAAGTTGACTATATTGACAGAATTAACATTCACATAACTTTAAt                                                                                                                                               >  2:2778880/2‑148 (MQ=255)

      gagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAAt                                                                                                                                               >  1:2319156/1‑148 (MQ=255)

         gAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTa                                                                                                                                            <  1:1983716/148‑1 (MQ=255)

         gAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTa                                                                                                                                            <  1:1286279/148‑1 (MQ=255)

          aaaGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTaa                                                                                                                                           >  1:2237674/1‑148 (MQ=255)

          aaaGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTaa                                                                                                                                           >  1:575218/1‑148 (MQ=255)

             ggACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGt                                                                                                                                        <  1:3056929/148‑1 (MQ=255)

             ggACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGt                                                                                                                                        <  1:1123773/148‑1 (MQ=255)

             ggACTAAAGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGt                                                                                                                                        <  1:1722711/148‑1 (MQ=255)

                  aaCGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatg                                                                                                                                   <  1:1403463/148‑1 (MQ=255)

                     gTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatgatg                                                                                                                                <  1:2694431/148‑1 (MQ=255)

                          tttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATgttgtt                                                                                                                           <  2:549110/148‑1 (MQ=255)

                          tttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATgttgtt                                                                                                                           >  2:2770371/1‑148 (MQ=255)

                           ttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGttt                                                                                                                          >  2:1662266/1‑148 (MQ=255)

                              aaaGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCg                                                                                                                       <  1:2111985/148‑1 (MQ=255)

                                aGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTg                                                                                                                     <  2:500656/148‑1 (MQ=255)

                                  aaTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTgag                                                                                                                   >  2:1820590/1‑148 (MQ=255)

                                  aaTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTgag                                                                                                                   >  1:1046577/1‑148 (MQ=255)

                                   aTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTgaga                                                                                                                  <  1:1299722/148‑1 (MQ=255)

                                   aTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTgaga                                                                                                                  <  1:1419559/148‑1 (MQ=255)

                                       aTTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACAt                                                                                                              >  1:471057/1‑148 (MQ=255)

                                       aTTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACAt                                                                                                              <  2:2625514/148‑1 (MQ=255)

                                        tttCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACAtt                                                                                                             <  1:1056737/148‑1 (MQ=255)

                                         ttCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTACATGATGTTGTTTGCGTGAGAACATtc                                                                                                            >  1:2818132/1‑148 (MQ=255)

                                          tcatcaTCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATtct                                                                                                           <  2:266855/148‑1 (MQ=255)

                                             tcatcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAg                                                                                                        >  2:1417764/1‑148 (MQ=255)

                                              catcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGc                                                                                                       >  1:1755730/1‑148 (MQ=255)

                                               atcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCa                                                                                                      <  1:936427/148‑1 (MQ=255)

                                                tcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCAt                                                                                                     <  2:1342496/148‑1 (MQ=255)

                                                tcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCAt                                                                                                     >  1:68136/1‑148 (MQ=255)

                                                 cTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCAtt                                                                                                    >  2:2288295/1‑148 (MQ=255)

                                                   gTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTaa                                                                                                  <  1:2022135/148‑1 (MQ=255)

                                                    ttAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAg                                                                                                 >  2:3233136/1‑148 (MQ=255)

                                                     taaactaaacTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAgg                                                                                                <  1:943963/148‑1 (MQ=255)

                                                     taaactaaacTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAgg                                                                                                <  2:1274220/148‑1 (MQ=255)

                                                      aaactaaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGa                                                                                               >  2:313554/1‑148 (MQ=255)

                                                      aaactaaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGa                                                                                               >  2:2410076/1‑148 (MQ=255)

                                                              ctCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAttttttt                                                                                       <  1:1224980/148‑1 (MQ=255)

                                                               tcatcaTTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTa                                                                                      <  1:1534533/148‑1 (MQ=255)

                                                                catcatTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTaa                                                                                     <  1:823190/148‑1 (MQ=255)

                                                                catcatTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTaa                                                                                     <  1:2482377/148‑1 (MQ=255)

                                                                catcatTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTaa                                                                                     >  2:2490028/1‑148 (MQ=255)

                                                                catcatTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTaa                                                                                     <  2:2948192/148‑1 (MQ=255)

                                                                cagcatGGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTaa                                                                                     <  2:3136176/145‑1 (MQ=255)

                                                                          aGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCtt                                                                           >  2:2147379/1‑148 (MQ=255)

                                                                          aGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCtt                                                                           <  1:583444/148‑1 (MQ=255)

                                                                           gATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTg                                                                          <  2:410840/148‑1 (MQ=255)

                                                                               gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGttt                                                                      <  1:2250814/148‑1 (MQ=255)

                                                                                 gagaTATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCt                                                                    >  2:1522150/1‑148 (MQ=255)

                                                                                  agaTATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCtt                                                                   <  2:1777225/148‑1 (MQ=255)

                                                                                     tataACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCttttt                                                                <  2:73374/148‑1 (MQ=255)

                                                                                     tataACTGATTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCttttt                                                                <  2:2895509/148‑1 (MQ=255)

                                                                                          cTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGt                                                           >  2:2130955/1‑148 (MQ=255)

                                                                                          cTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGt                                                           <  1:3158758/148‑1 (MQ=255)

                                                                                           tGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTc                                                          <  2:317752/148‑1 (MQ=255)

                                                                                           tGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTc                                                          >  1:1506592/1‑148 (MQ=255)

                                                                                            gTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTcc                                                         >  1:1039487/1‑148 (MQ=255)

                                                                                              ttttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGa                                                       <  1:557404/148‑1 (MQ=255)

                                                                                               tttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGaa                                                      <  2:2103270/148‑1 (MQ=255)

                                                                                               tttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCAGaa                                                      >  2:1996125/1‑148 (MQ=255)

                                                                                                ttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAt                                                     >  1:1895765/1‑148 (MQ=255)

                                                                                                     tttACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACa                                                <  1:2116576/148‑1 (MQ=255)

                                                                                                      ttACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACaa                                               >  1:1025265/1‑148 (MQ=255)

                                                                                                      ttACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACaa                                               <  1:373055/148‑1 (MQ=255)

                                                                                                      ttACTATTTACTTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACaa                                               <  2:2272010/148‑1 (MQ=255)

                                                                                                         ctctTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAatat                                            <  2:1442875/148‑1 (MQ=255)

                                                                                                             ttACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATg                                        >  1:1883080/1‑148 (MQ=255)

                                                                                                             ttACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATg                                        <  2:758083/148‑1 (MQ=255)

                                                                                                             ttACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATg                                        <  1:2957337/148‑1 (MQ=255)

                                                                                                                  ttGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCatat                                   >  2:3048673/1‑148 (MQ=255)

                                                                                                                   tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCAtata                                  >  1:1972372/1‑148 (MQ=255)

                                                                                                                    gACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAg                                 <  1:1662266/148‑1 (MQ=255)

                                                                                                                        ttATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                             >  1:2968726/1‑148 (MQ=255)

                                                                                                                          aTTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAtt                           <  2:575218/148‑1 (MQ=255)

                                                                                                                           ttGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAttt                          <  1:1820590/148‑1 (MQ=255)

                                                                                                                           ttGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAttt                          <  1:1008991/148‑1 (MQ=255)

                                                                                                                            tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         >  1:2587119/1‑148 (MQ=255)

                                                                                                                            tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         <  1:2511476/148‑1 (MQ=255)

                                                                                                                            tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         <  2:1036212/148‑1 (MQ=255)

                                                                                                                            tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         <  1:152327/148‑1 (MQ=255)

                                                                                                                            tGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         <  2:68136/148‑1 (MQ=255)

                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  2:2862303/148‑1 (MQ=255)

                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        >  1:3299832/1‑148 (MQ=255)

                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        >  1:3300029/1‑148 (MQ=255)

                                                                                                                             gACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  1:3007680/148‑1 (MQ=255)

                                                                                                                             gACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  1:1180491/148‑1 (MQ=255)

                                                                                                                               cAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATg                      >  2:537566/1‑148 (MQ=255)

                                                                                                                                aGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGa                     >  1:262059/1‑148 (MQ=255)

                                                                                                                                  aaTTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAAt                   <  1:1770012/148‑1 (MQ=255)

                                                                                                                                   aTTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATc                  <  1:3136849/148‑1 (MQ=255)

                                                                                                                                       aCATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCg              >  2:315969/1‑148 (MQ=255)

                                                                                                                                        cATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATATCATGAATCTGCGa             >  2:1973118/1‑148 (MQ=255)

                                                                                                                                            cacaTATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGGGAAAAt         <  1:1473684/148‑1 (MQ=255)

                                                                                                                                            cacaTATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAAt         <  1:2538730/148‑1 (MQ=255)

                                                                                                                                               atatCTTTAATTTAATGTCCATGATGGTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTTGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      <  2:843591/148‑1 (MQ=255)

                                                                                                                                                 atCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAAt    >  2:1129826/1‑148 (MQ=255)

                                                                                                                                                   cTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt  <  2:2572111/148‑1 (MQ=255)

TCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGT  >  CP009273/2983421‑2983715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`