BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,018,544	G→T	89.5%	A279S (GCC→TCC)	xanQ →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,018,544	0	G	T	89.5%	604.8 / 64.7	236	A279S (GCC→TCC)	xanQ	xanthine permease
Reads supporting (aligned to +/- strand): ref base G (13/12); new base T (105/106); total (118/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGG  >  CP009273/3018400‑3018690

gtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCa                                                                                                                                                 >  1:936551/1‑148 (MQ=255)

      ttCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTGTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCaa                                                                                                                                           >  2:858187/1‑148 (MQ=255)

          gcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGt                                                                                                                                       >  1:3110086/1‑148 (MQ=255)

          gcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGt                                                                                                                                       >  1:1147165/1‑148 (MQ=255)

             gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTc                                                                                                                                    <  1:1440700/148‑1 (MQ=255)

              tATGCGCAATATGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                   >  1:912001/1‑148 (MQ=255)

                 gcgcAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCccgc                                                                                                                                >  1:233243/1‑148 (MQ=255)

                 gcgcAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCccgc                                                                                                                                >  1:1808748/1‑148 (MQ=255)

                 gcgcAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCccgc                                                                                                                                >  1:2716594/1‑148 (MQ=255)

                       tttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGcccc                                                                                                                          >  1:2829757/1‑148 (MQ=255)

                       tttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGcccc                                                                                                                          >  2:229886/1‑148 (MQ=255)

                        ttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCa                                                                                                                         <  1:3023611/148‑1 (MQ=255)

                         tGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCAt                                                                                                                        <  1:2140403/148‑1 (MQ=255)

                             gTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAg                                                                                                                    >  1:1056987/1‑148 (MQ=255)

                              ttAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTGCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCCgg                                                                                                                   >  2:1108891/1‑148 (MQ=255)

                               tAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggg                                                                                                                  <  1:586198/148‑1 (MQ=255)

                               tAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggg                                                                                                                  <  1:388309/148‑1 (MQ=255)

                               tAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggg                                                                                                                  <  1:136985/148‑1 (MQ=255)

                                 aTCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggggg                                                                                                                >  1:1781380/1‑148 (MQ=255)

                                  tCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGa                                                                                                               <  1:2028976/148‑1 (MQ=255)

                                   cACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGaa                                                                                                              <  1:1978009/148‑1 (MQ=255)

                                        tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                         <  2:339321/148‑1 (MQ=255)

                                         cccGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTAt                                                                                                        <  1:1837657/148‑1 (MQ=255)

                                          ccGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATc                                                                                                       <  1:2441625/148‑1 (MQ=255)

                                           cGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCa                                                                                                      >  1:3206374/1‑148 (MQ=255)

                                            gCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAg                                                                                                     >  1:805607/1‑148 (MQ=255)

                                             cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                    <  1:2815425/148‑1 (MQ=255)

                                             cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                    <  1:1162012/148‑1 (MQ=255)

                                             cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                    >  1:259652/1‑148 (MQ=255)

                                             cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                    <  1:1463242/148‑1 (MQ=255)

                                              aTCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCTACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTc                                                                                                   <  2:221950/148‑1 (MQ=255)

                                               tCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTcc                                                                                                  >  1:3309279/1‑148 (MQ=255)

                                                ccGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTccc                                                                                                 >  2:1878661/1‑148 (MQ=255)

                                                ccGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTccc                                                                                                 >  2:489716/1‑148 (MQ=255)

                                                       aaTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGaa                                                                                          <  1:3239216/148‑1 (MQ=255)

                                                        aTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGaaa                                                                                         >  2:3051537/1‑148 (MQ=255)

                                                          aCGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAgg                                                                                       >  2:2766383/1‑148 (MQ=255)

                                                          aCGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAgg                                                                                       <  1:3286975/148‑1 (MQ=255)

                                                          aCGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAgg                                                                                       >  2:942852/1‑148 (MQ=255)

                                                          aCGGCTTTAGAATTAGCTTCCATAACTTCCTCGTGTATGTCACGATTTAACTGCTTATCGTGCTGGAATCAGTCGGCGATAACACCTCCACGGCAATGGTTTCCCCCCGCCACATTCAGGGGGAAGAGTATCAGTACCGGATGAAAgg                                                                                       >  2:2142854/1‑148 (MQ=255)

                                                             gCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcgg                                                                                    >  1:993429/1‑148 (MQ=255)

                                                                ttAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGt                                                                                 >  1:2021855/1‑148 (MQ=255)

                                                                 tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTg                                                                                >  2:1421383/1‑148 (MQ=255)

                                                                 tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTg                                                                                <  2:718035/148‑1 (MQ=255)

                                                                 tAGTTTTAGCGTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTg                                                                                <  2:2378700/148‑1 (MQ=255)

                                                                     tttAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCACTGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTgg                                                                            >  2:3154442/1‑148 (MQ=255)

                                                                        aGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAg                                                                         <  1:2197188/148‑1 (MQ=255)

                                                                        aGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAg                                                                         <  2:1455694/148‑1 (MQ=255)

                                                                            tCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATgg                                                                     <  2:1692980/148‑1 (MQ=255)

                                                                            tCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATgg                                                                     <  2:1223968/148‑1 (MQ=255)

                                                                            tCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATgg                                                                     >  1:2904623/1‑148 (MQ=255)

                                                                              cATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTc                                                                   >  2:867839/1‑148 (MQ=255)

                                                                                 cAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTgg                                                                >  2:2955627/1‑148 (MQ=255)

                                                                                  aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGt                                                               >  1:3258106/1‑148 (MQ=255)

                                                                                  aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGt                                                               <  2:2994898/148‑1 (MQ=255)

                                                                                  aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGt                                                               <  2:1046744/148‑1 (MQ=255)

                                                                                    ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                             <  1:2586571/148‑1 (MQ=255)

                                                                                    ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                             >  2:71112/1‑148 (MQ=255)

                                                                                    ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                             >  1:2616581/1‑148 (MQ=255)

                                                                                     tCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTc                                                            <  2:912001/148‑1 (MQ=255)

                                                                                     tCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTc                                                            <  1:2916089/148‑1 (MQ=255)

                                                                                        tggtggTTGGATCGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGt                                                         <  1:2761634/148‑1 (MQ=255)

                                                                                         ggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                        <  2:2479112/148‑1 (MQ=255)

                                                                                         ggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                        >  2:162699/1‑148 (MQ=255)

                                                                                         ggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                        <  1:1840493/148‑1 (MQ=255)

                                                                                           tggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTAt                                                      <  1:229886/148‑1 (MQ=255)

                                                                                               tGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGcc                                                  <  2:2027032/148‑1 (MQ=255)

                                                                                                 gCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                                >  1:2267558/1‑148 (MQ=255)

                                                                                                      aTTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTg                                           >  2:2881794/1‑148 (MQ=255)

                                                                                                      aTTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTg                                           >  1:508507/1‑148 (MQ=255)

                                                                                                        ttATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTc                                         >  2:1768891/1‑148 (MQ=255)

                                                                                                        ttATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTc                                         >  2:1768740/1‑148 (MQ=255)

                                                                                                         tATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCg                                        <  1:1415938/148‑1 (MQ=255)

                                                                                                           tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATTGCCTCCGCTGTCGGt                                      <  2:2433404/148‑1 (MQ=255)

                                                                                                           tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGt                                      >  2:886722/1‑148 (MQ=255)

                                                                                                            cTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGtt                                     <  2:3232930/148‑1 (MQ=255)

                                                                                                             tGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTc                                    <  2:1171547/148‑1 (MQ=255)

                                                                                                              gCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCa                                   <  1:1011409/148‑1 (MQ=255)

                                                                                                                ttAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAtt                                 >  1:1555105/1‑148 (MQ=255)

                                                                                                                ttAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAtt                                 >  2:3079763/1‑148 (MQ=255)

                                                                                                                 tAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTa                                >  1:317493/1‑148 (MQ=255)

                                                                                                                 tAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTa                                <  2:1681673/148‑1 (MQ=255)

                                                                                                                    cGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCa                             <  2:548659/148‑1 (MQ=255)

                                                                                                                     gTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCAt                            <  2:2344884/148‑1 (MQ=255)

                                                                                                                            aaGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCAc                     >  2:1465738/1‑148 (MQ=255)

                                                                                                                              gCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGt                   >  1:1474447/1‑148 (MQ=255)

                                                                                                                              gCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGt                   <  1:1407673/148‑1 (MQ=255)

                                                                                                                                 gTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTg                >  1:733342/1‑148 (MQ=255)

                                                                                                                                 gTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTg                >  1:2319996/1‑148 (MQ=255)

                                                                                                                                      cGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaa           >  1:3151543/1‑148 (MQ=255)

                                                                                                                                      cGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaa           <  2:2616581/148‑1 (MQ=255)

                                                                                                                                       gATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaaa          <  1:1431429/148‑1 (MQ=255)

                                                                                                                                        atatCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaaaa         <  1:1032512/148‑1 (MQ=255)

                                                                                                                                          atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaata       >  2:286085/1‑148 (MQ=255)

                                                                                                                                          atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaata       <  2:134788/148‑1 (MQ=255)

                                                                                                                                          atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaata       <  2:183028/148‑1 (MQ=255)

                                                                                                                                          atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCAGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACAATTAACCACGTTTGCGCAAaata       >  2:671487/1‑148 (MQ=255)

                                                                                                                                           tCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataa      <  2:3104201/148‑1 (MQ=255)

                                                                                                                                            cACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataat     <  2:397284/148‑1 (MQ=255)

                                                                                                                                            cACCTCAACGGCAATTGTTTCCCGCCGCCCCATTCAGGGGGATGTGTATCAGTCCCGGGTGAAATGCGTCGTGCTGGCAGATGTTCTTGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCATattaat     <  1:2985703/148‑1 (MQ=255)

GTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGG  >  CP009273/3018400‑3018690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`