Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,024,081 A→C 92.3% V70V (GTT→GTG)  ygfT ← putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,024,0810AC92.3% 618.6 / 19.3 169V70V (GTT→GTG) ygfTputative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (10/3);  new base C (97/59);  total (107/62)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 169 total aligned reads displayed.

GCGCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACC  >  CP009273/3023937‑3024222
                                                                                                                                                |                                                                                                                                             
gcgcTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCAcc                                                                                                                                            >  2:212027/1‑148 (MQ=255)
  gcTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCgg                                                                                                                                          <  1:2204884/148‑1 (MQ=255)
     ggTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACa                                                                                                                                       >  2:1501198/1‑148 (MQ=255)
           aaaGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGt                                                                                                                                 <  2:1311726/148‑1 (MQ=255)
            aaGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTa                                                                                                                                >  1:470042/1‑148 (MQ=255)
             aGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTaa                                                                                                                               >  1:1143329/1‑148 (MQ=255)
             aGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTaa                                                                                                                               <  2:2993063/148‑1 (MQ=255)
              ggTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAAc                                                                                                                              >  2:1443059/1‑148 (MQ=255)
              ggTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAAc                                                                                                                              >  2:1081246/1‑148 (MQ=255)
                 cGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCa                                                                                                                           >  1:2804883/1‑148 (MQ=255)
                 cGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCa                                                                                                                           <  1:1632033/148‑1 (MQ=255)
                    aTTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgg                                                                                                                        <  2:3227950/148‑1 (MQ=255)
                    aTTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgg                                                                                                                        <  1:2544646/148‑1 (MQ=255)
                     tttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAggg                                                                                                                       >  1:2463770/1‑148 (MQ=255)
                      ttCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAgggg                                                                                                                      >  1:3113822/1‑148 (MQ=255)
                         tGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCACGGGGCAt                                                                                                                   >  2:1083713/1‑148 (MQ=255)
                           cgcAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCAttg                                                                                                                 <  2:2997712/148‑1 (MQ=255)
                                tCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCa                                                                                                            <  1:2403886/148‑1 (MQ=255)
                                tCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCa                                                                                                            <  1:2403766/148‑1 (MQ=255)
                                 cGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAg                                                                                                           >  1:1665136/1‑148 (MQ=255)
                                  gTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt                                                                                                          <  1:2778945/148‑1 (MQ=255)
                                   tATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg                                                                                                         >  2:206136/1‑148 (MQ=255)
                                   tATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg                                                                                                         >  2:2550373/1‑148 (MQ=255)
                                   tATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg                                                                                                         >  2:1226829/1‑148 (MQ=255)
                                   tATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg                                                                                                         <  1:3147459/148‑1 (MQ=255)
                                    aTCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGa                                                                                                        >  1:2572309/1‑148 (MQ=255)
                                      cGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATg                                                                                                      >  1:3007096/1‑148 (MQ=255)
                                      cGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATg                                                                                                      <  2:514687/148‑1 (MQ=255)
                                      cGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATg                                                                                                      >  2:1310698/1‑148 (MQ=255)
                                        aCCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGc                                                                                                    <  1:3104843/148‑1 (MQ=255)
                                            tctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGc                                                                                                >  1:3120361/1‑148 (MQ=255)
                                               cAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCAc                                                                                             >  2:1080061/1‑148 (MQ=255)
                                               cAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCAc                                                                                             >  2:3056502/1‑148 (MQ=255)
                                               cAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCAc                                                                                             >  2:1116596/1‑148 (MQ=255)
                                                   gacgCCACAGGGGCAAGCGATTGCGCATCATTTACAACCAATACCTTTGTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGa                                                                                         <  2:2108615/148‑1 (MQ=255)
                                                   gacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGa                                                                                         <  2:2107747/148‑1 (MQ=255)
                                                    acgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGAt                                                                                        >  2:285406/1‑148 (MQ=255)
                                                       ccAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCg                                                                                     <  1:212027/148‑1 (MQ=255)
                                                           aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                 >  2:1302213/1‑148 (MQ=255)
                                                           aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                 >  2:196065/1‑148 (MQ=255)
                                                           aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                 >  1:1417703/1‑148 (MQ=255)
                                                           aGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGc                                                                                 <  2:394059/148‑1 (MQ=255)
                                                            ggggCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGcc                                                                                >  2:3076390/1‑148 (MQ=255)
                                                                  aGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCt                                                                          >  1:1844543/1‑148 (MQ=255)
                                                                   gCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCtt                                                                         >  2:3287606/1‑148 (MQ=255)
                                                                   gCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCtt                                                                         <  1:2062904/148‑1 (MQ=255)
                                                                    cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCttt                                                                        >  1:3152342/1‑148 (MQ=255)
                                                                    cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCttt                                                                        >  1:1511905/1‑148 (MQ=255)
                                                                      aTTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTcc                                                                      >  1:1001335/1‑148 (MQ=255)
                                                                      aTTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTcc                                                                      >  1:1001342/1‑148 (MQ=255)
                                                                        tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCt                                                                    >  1:733767/1‑148 (MQ=255)
                                                                        tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCt                                                                    <  1:214554/148‑1 (MQ=255)
                                                                        tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCt                                                                    >  2:1851358/1‑148 (MQ=255)
                                                                           gcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACa                                                                 <  1:1051931/148‑1 (MQ=255)
                                                                            cATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACaa                                                                >  1:2813549/1‑148 (MQ=255)
                                                                              tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACg                                                              <  2:1621726/148‑1 (MQ=255)
                                                                               cTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGt                                                             >  2:320814/1‑148 (MQ=255)
                                                                                 tttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTgg                                                           >  1:494028/1‑148 (MQ=255)
                                                                                 tttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTgg                                                           <  1:1501198/148‑1 (MQ=255)
                                                                                     cAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATAc                                                       >  1:324748/1‑148 (MQ=255)
                                                                                         cAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCgg                                                   >  2:2643341/1‑148 (MQ=255)
                                                                                               aTTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaaa                                             <  1:285406/148‑1 (MQ=255)
                                                                                                tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGACAg                                            >  2:1839371/1‑148 (MQ=255)
                                                                                                 ttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGt                                           >  2:2465102/1‑148 (MQ=255)
                                                                                                  tttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTc                                          <  1:1080061/148‑1 (MQ=255)
                                                                                                  tttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTc                                          <  2:597276/148‑1 (MQ=255)
                                                                                                   gtGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCa                                         <  1:326419/147‑1 (MQ=255)
                                                                                                    tGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCAc                                        >  1:3218397/1‑148 (MQ=255)
                                                                                                       tCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACtgt                                     >  2:1774019/1‑148 (MQ=255)
                                                                                                        cGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACtgtg                                    >  1:69595/1‑148 (MQ=255)
                                                                                                           ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACt                                 >  1:2074247/1‑148 (MQ=255)
                                                                                                           ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACt                                 <  1:285497/148‑1 (MQ=255)
                                                                                                           ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACt                                 >  1:2916853/1‑148 (MQ=255)
                                                                                                               ttGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGc                             >  2:1329455/1‑148 (MQ=255)
                                                                                                                tGTACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCg                            >  1:1602268/1‑148 (MQ=255)
                                                                                                                 gTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCgg                           <  1:1969576/148‑1 (MQ=255)
                                                                                                                  tACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGc                          >  1:582486/1‑148 (MQ=255)
                                                                                                                  tACGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGc                          >  1:3044791/1‑148 (MQ=255)
                                                                                                                   aCGCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGcc                         >  1:1181989/1‑148 (MQ=255)
                                                                                                                     gCTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAg                       <  1:1076633/148‑1 (MQ=255)
                                                                                                                      cTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGt                      >  2:878996/1‑148 (MQ=255)
                                                                                                                      cTATCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGt                      >  2:2914695/1‑148 (MQ=255)
                                                                                                                        ctCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGttt                    <  2:1700521/147‑1 (MQ=255)
                                                                                                                        aTCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGttt                    <  2:2583887/148‑1 (MQ=255)
                                                                                                                         tCGGACTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGtttt                   >  2:1016388/1‑148 (MQ=255)
                                                                                                                             aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTg               <  2:2289289/148‑1 (MQ=255)
                                                                                                                             aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTg               <  2:2289268/148‑1 (MQ=255)
                                                                                                                             aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTg               <  2:1467320/148‑1 (MQ=255)
                                                                                                                             aCTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTGTTCTTg               >  2:1602260/1‑148 (MQ=255)
                                                                                                                              cTGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa              <  2:733767/148‑1 (MQ=255)
                                                                                                                               tGGAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAt             >  1:80655/1‑148 (MQ=255)
                                                                                                                                ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt            <  1:2381825/148‑1 (MQ=255)
                                                                                                                                ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt            <  1:2381736/148‑1 (MQ=255)
                                                                                                                                ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt            >  2:465589/1‑148 (MQ=255)
                                                                                                                                ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt            >  2:465736/1‑148 (MQ=255)
                                                                                                                                ggAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGAtt            >  2:775197/1‑148 (MQ=255)
                                                                                                                                 gAAAGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtg           >  1:3195222/1‑148 (MQ=255)
                                                                                                                                    aGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtgt        >  1:1801972/1‑148 (MQ=255)
                                                                                                                                    aGTCAGAGCATTCACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtgt        >  1:130795/1‑148 (MQ=255)
                                                                                                                                          agCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCAcc  >  1:3013599/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GCGCTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACC  >  CP009273/3023937‑3024222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑