Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,592 T→A 30.2% N292Y (AAT→TAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,5920TA30.2% 280.0 / 167.4 179N292Y (AAT→TAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base T (62/63);  new base A (27/27);  total (89/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 188 total aligned reads displayed.

GGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTAT  >  CP009273/3030446‑3030738
                                                                                                                                                  |                                                                                                                                                  
ggCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTg                                                                                                                                                   >  2:3073445/1‑148 (MQ=255)
ggCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTg                                                                                                                                                   >  2:3154499/1‑148 (MQ=255)
  cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTgag                                                                                                                                                 >  1:1021437/1‑148 (MQ=255)
  cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTgag                                                                                                                                                 <  1:3269101/148‑1 (MQ=255)
     cAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACg                                                                                                                                              <  2:1539449/148‑1 (MQ=255)
      aaTTTGCATTCCTTGTTCGATCTCTTTTAGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATCTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGt                                                                                                                                             >  2:857308/1‑148 (MQ=255)
        tttGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTgg                                                                                                                                           >  1:2689038/1‑148 (MQ=255)
        tttGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTgg                                                                                                                                           >  1:2098949/1‑148 (MQ=255)
         ttGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGc                                                                                                                                          >  1:205963/1‑148 (MQ=255)
            cATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcccc                                                                                                                                       >  2:1463315/1‑148 (MQ=255)
              ttCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAg                                                                                                                                     >  2:306987/1‑148 (MQ=255)
                    gTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCaaa                                                                                                                               <  2:1323515/148‑1 (MQ=255)
                     ttCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCaaaa                                                                                                                              >  2:1283686/1‑148 (MQ=255)
                      tCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAAc                                                                                                                             >  1:1071613/1‑148 (MQ=255)
                         aTCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTa                                                                                                                          >  1:1282002/1‑148 (MQ=255)
                          tctcTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTaa                                                                                                                         >  1:2962563/1‑148 (MQ=255)
                           ctctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTaaa                                                                                                                        >  1:903440/1‑148 (MQ=255)
                            tctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAAt                                                                                                                       <  2:2069821/148‑1 (MQ=255)
                            tctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAAt                                                                                                                       <  2:2070178/148‑1 (MQ=255)
                              ttttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCg                                                                                                                     <  2:1105697/148‑1 (MQ=255)
                              ttttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCg                                                                                                                     <  2:3275181/148‑1 (MQ=255)
                               tttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGc                                                                                                                    >  1:1575488/1‑148 (MQ=255)
                                      tCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcggc                                                                                                             >  2:2195621/1‑148 (MQ=255)
                                       cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                            <  2:594413/148‑1 (MQ=255)
                                       cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                            >  1:1601333/1‑148 (MQ=255)
                                        tGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGa                                                                                                           <  2:1631783/148‑1 (MQ=255)
                                        tGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGa                                                                                                           <  2:1631683/148‑1 (MQ=255)
                                         ggTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCAAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAg                                                                                                          <  1:2267473/148‑1 (MQ=255)
                                          ttttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGAACAACATCAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                         <  1:2150045/147‑1 (MQ=255)
                                          gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                         <  2:2245981/148‑1 (MQ=255)
                                          gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTACATCGCTGGCGGCGAGt                                                                                                         <  2:379203/148‑1 (MQ=255)
                                           tttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGtt                                                                                                        <  2:516318/148‑1 (MQ=255)
                                            ttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttt                                                                                                       <  1:1921881/148‑1 (MQ=255)
                                               gcgcATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTtg                                                                                                    <  1:779775/148‑1 (MQ=255)
                                                cgcATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTtgt                                                                                                   >  2:747925/1‑148 (MQ=255)
                                                 gcATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTtgtg                                                                                                  <  1:631557/148‑1 (MQ=255)
                                                  cATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGc                                                                                                 >  1:67054/1‑148 (MQ=255)
                                                    tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCAt                                                                                               <  1:1008696/148‑1 (MQ=255)
                                                    tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCAt                                                                                               >  2:795543/1‑148 (MQ=255)
                                                    tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCAt                                                                                               <  1:1008726/148‑1 (MQ=255)
                                                     cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                              >  1:302638/1‑148 (MQ=255)
                                                     cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                              <  1:131516/148‑1 (MQ=255)
                                                        gTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACg                                                                                           >  2:502670/1‑148 (MQ=255)
                                                         ttCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACgg                                                                                          >  2:1177947/1‑148 (MQ=255)
                                                         ttCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACgg                                                                                          >  1:1464015/1‑148 (MQ=255)
                                                          tCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGt                                                                                         <  1:38795/148‑1 (MQ=255)
                                                             tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                      >  2:1875689/1‑148 (MQ=255)
                                                              gtGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGc                                                                                     <  2:1776890/147‑1 (MQ=255)
                                                                gCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCa                                                                                   <  1:105461/148‑1 (MQ=255)
                                                                 ccAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCAc                                                                                  >  2:2742886/1‑148 (MQ=255)
                                                                   aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                                >  2:3254574/1‑148 (MQ=255)
                                                                   aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                                >  2:1759152/1‑148 (MQ=255)
                                                                   aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                                >  2:3255812/1‑148 (MQ=255)
                                                                    gCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTc                                                                               >  2:867801/1‑148 (MQ=255)
                                                                     cACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGTGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCa                                                                              <  1:3174608/148‑1 (MQ=255)
                                                                     cACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCa                                                                              >  2:2297019/1‑148 (MQ=255)
                                                                      aCGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCaa                                                                             <  2:2510185/148‑1 (MQ=255)
                                                                        gcgcgcTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAagc                                                                           <  2:1282002/148‑1 (MQ=255)
                                                                              ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                     <  2:59014/148‑1 (MQ=255)
                                                                              ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                     >  2:476452/1‑148 (MQ=255)
                                                                               tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                    <  1:810943/148‑1 (MQ=255)
                                                                                 gCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTa                                                                  <  1:1471624/148‑1 (MQ=255)
                                                                                     aTGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcc                                                              >  1:182315/1‑148 (MQ=255)
                                                                                     aTGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcc                                                              >  2:252684/1‑148 (MQ=255)
                                                                                     aTGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcc                                                              >  1:1415381/1‑148 (MQ=255)
                                                                                        ttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCg                                                           <  1:668907/148‑1 (MQ=255)
                                                                                         tgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCgg                                                          <  2:273878/148‑1 (MQ=255)
                                                                                             gCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACgg                                                      <  1:1177947/148‑1 (MQ=255)
                                                                                                 aacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACt                                                  <  2:2136481/148‑1 (MQ=255)
                                                                                                       aGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCgg                                            <  1:2720146/148‑1 (MQ=255)
                                                                                                         cgcCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCt                                          <  2:2972109/148‑1 (MQ=255)
                                                                                                            cacaCCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgg                                       <  1:1342513/148‑1 (MQ=255)
                                                                                                            cacaCCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgg                                       <  1:506439/148‑1 (MQ=255)
                                                                                                            cacaCCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgg                                       <  1:857308/148‑1 (MQ=255)
                                                                                                              cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGAt                                     >  1:694374/1‑148 (MQ=255)
                                                                                                                ccGACGGACATATCGTCCAGTCGTCCGGCAGCAATGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGc                                   >  1:2707998/1‑148 (MQ=255)
                                                                                                                  gACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGa                                 <  2:693942/148‑1 (MQ=255)
                                                                                                                   aCGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGAc                                >  2:1390967/1‑148 (MQ=255)
                                                                                                                    cGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                               >  2:233442/1‑148 (MQ=255)
                                                                                                                       aCATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAt                            >  2:560636/1‑148 (MQ=255)
                                                                                                                       aCATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAt                            >  1:1445598/1‑148 (MQ=255)
                                                                                                                          tatCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATccc                         <  1:2331390/148‑1 (MQ=255)
                                                                                                                          tatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATccc                         <  1:476443/148‑1 (MQ=255)
                                                                                                                           atCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                        <  1:762651/148‑1 (MQ=255)
                                                                                                                           atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                        <  2:380896/148‑1 (MQ=255)
                                                                                                                           atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                        <  2:380916/148‑1 (MQ=255)
                                                                                                                           atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                        <  2:2045906/148‑1 (MQ=255)
                                                                                                                              gTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGc                     <  1:2582829/148‑1 (MQ=255)
                                                                                                                                ccAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCa                   >  1:1306161/1‑148 (MQ=255)
                                                                                                                                ccAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCa                   >  2:2570124/1‑148 (MQ=255)
                                                                                                                                        ccGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCTTCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGaa           <  2:1179005/148‑1 (MQ=255)
                                                                                                                                            cagcaTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCg       >  2:2414649/1‑148 (MQ=255)
                                                                                                                                            cagcaTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCg       >  1:595978/1‑148 (MQ=255)
                                                                                                                                               caTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGatt    >  2:1575496/1‑148 (MQ=255)
                                                                                                                                               caTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGatt    <  2:1575488/148‑1 (MQ=255)
                                                                                                                                                 ttGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGattat  >  1:2913687/1‑148 (MQ=255)
                                                                                                                                                 ttGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGattat  <  1:3254574/148‑1 (MQ=255)
                                                                                                                                                 ttGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGattat  <  1:1759152/148‑1 (MQ=255)
                                                                                                                                                 ttGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGattat  <  1:1759175/148‑1 (MQ=255)
                                                                                                                                                 ttGAGACGTGGCCCCAGCGCAAAACCTAAATCGCCGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGattat  <  1:307224/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTAT  >  CP009273/3030446‑3030738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑